Abstract:
:Episodes of cholera stemming from indigenous Vibrio cholerae strains in Australia are mainly associated with environmental sources. In the present study, 10 V. cholerae O1 strains of Australian origin were characterized. All of the strains were serogroup O1 and their conventional phenotypic traits categorized them as belonging to the El Tor biotype. Genetic screening of 12 genomic regions that are associated with virulence in V. cholerae showed variable results. Analysis of the ctxAB gene showed that the Australian environmental reservoir contains both toxigenic and non-toxigenic V. cholerae strains. DNA sequencing revealed that all of the toxigenic V. cholerae strains examined were of ctxB genotype 2. Whole genome PFGE analysis revealed that the environmental toxigenic V. cholerae O1 strains were more diverse than the non-toxigenic environmental O1 strains, and the absence of genes that make up the Vibrio seventh pandemic island-I and -II in all of the strains indicates their pre-seventh pandemic ancestry.
journal_name
J Med Microbioljournal_title
Journal of medical microbiologyauthors
Safa A,Bhuiyan NA,Murphy D,Bates J,Nusrin S,Kong RYC,Chongsanguan M,Chaicumpa W,Nair GBdoi
10.1099/jmm.0.004333-0subject
Has Abstractpub_date
2009-01-01 00:00:00pages
105-111issue
Pt 1eissn
0022-2615issn
1473-5644journal_volume
58pub_type
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