Utility of real-time amplification of selected 16S rRNA gene sequences as a tool for detection and identification of microbial signatures directly from clinical samples.

abstract：:In order to develop novel host/pathogen real-time PCR assays for routine diagnostic use, early gene expression patterns from both Epstein-Barr virus (EBV) and Raji cells were examined after inducing the lytic life cycle using 12-O-tetradecanoyl-13-phorbol ester and sodium butyrate. Real-time PCR identified several hig...

journal_title：Journal of medical microbiology

authors： Auburn H,Zuckerman M,Smith M

更新日期：2016-11-01 00:00:00

abstract：PURPOSE:The current study has been designed to delineate the efficacy of geraniol (GE) on biofilm formation in Staphylococcus epidermidis as well as the effect of subinhibitory concentrations of GE on the development of adaptive resistance. METHODOLOGY:Biofilm biomass quantification assay was performed to evaluate the...

journal_title：Journal of medical microbiology

authors： Kannappan A,Sivaranjani M,Srinivasan R,Rathna J,Pandian SK,Ravi AV

更新日期：2017-10-01 00:00:00

abstract：:Motile curved rods seen in vaginal secretions have been isolated on Columbia agar supplemented with 5% human blood and vitamin K. Growth occurred anaerobically and in 5% oxygen but not in more aerobic conditions. There were two distinct groups of these organisms, distinguishable by morphology, biochemical activity and...

journal_title：Journal of medical microbiology

authors： Sprott MS,Ingham HR,Pattman RS,Eisenstadt RL,Short GR,Narang HK,Sisson PR,Selkon JB

更新日期：1983-05-01 00:00:00

abstract：:Campylobacter fetus subsp. fetus infection can occur in female sheep, causing infertility or abortion. Despite extensive research on the effect of these bacteria on female fertility, little research has been done on the influence of C. fetus subsp. fetus on the male factor. Our objective was to examine the influence o...

journal_title：Journal of medical microbiology

authors： Zan Bar T,Yehuda R,Hacham T,Krupnik S,Bartoov B

更新日期：2008-11-01 00:00:00

abstract：:The effect of passive immunotherapy with antisera against heat-killed Pseudomonas aeruginosa and three of its exo-enzymes (elastase, alkaline protease and exotoxin A) in gut-derived P. aeruginosa sepsis was evaluated. Mice were given a suspension of P. aeruginosa strain D4 in their drinking water, together with ampici...

journal_title：Journal of medical microbiology

authors： Matsumoto T,Furuya N,Tateda K,Miyazaki S,Ohno A,Ishii Y,Hirakata Y,Yamaguchi K

更新日期：1999-08-01 00:00:00

abstract：:The potential of human plasma (HP) or serum (HS) as a replacement for fetal calf serum (FCS) was evaluated in a liver infusion tryptose (LIT) medium for bulk cultivation of Leishmania donovani promastigotes. The promastigote yield with the LIT-FCS standard medium was 0.4-1.8×10(7) ml(-1), and yields of 0.5-3.4×10(7) (...

journal_title：Journal of medical microbiology

authors： Mahamoud A,Osman HA,Mansour D,El Harith A

更新日期：2013-08-01 00:00:00

abstract：:Infection of humans by Abiotrophia defectiva, a nutritionally variant streptococcus, most commonly takes the form of endocarditis, though a variety of other manifestations ranging from central nervous system abscesses to orthopaedic infections have been seen. We report here what we believe is the first case of bullous...

journal_title：Journal of medical microbiology

authors： Anderson HM,Miller C,Kemp E,Huntington MK

更新日期：2012-07-01 00:00:00

abstract：:The resistance of Balamuthia mandrillaris to physical, chemical and radiological conditions was tested. Following treatments, viability was determined by culturing amoebae on human brain microvascular endothelial cells for up to 12 days. B. mandrillaris cysts were resistant to repeated freeze-thawing (five times), tem...

journal_title：Journal of medical microbiology

authors： Siddiqui R,Ortega-Rivas A,Khan NA

更新日期：2008-04-01 00:00:00

abstract：:Phenotypic loss of protein A production was tested in six methicillin-resistant (McR) Staphylococcus aureus (MRSA) isolates and their isogenic methicillin-sensitive (McS) variants by a radiolabelled IgG-binding assay with washed cells and by Western blotting of supernates prepared from lysed washed cells. Genomic DNA ...

journal_title：Journal of medical microbiology

authors： Poston SM,Glancey GR,Wyatt JE,Hogan T,Foster TJ

更新日期：1993-12-01 00:00:00

abstract：:Antibody (AMI) and cell-mediated (CMI) immunity to rubella virus (RV) were evaluated in healthy adolescent males (n = 11) and females (n = 28) undergoing routine reimmunisation with RA27/3 strain RV as a component of measles-mumps-rubella (MMR) vaccine. Blood samples were collected just before and at 2, 4 and 10 weeks...

journal_title：Journal of medical microbiology

authors： Mitchell LA

更新日期：1999-12-01 00:00:00

abstract：:The present study aimed to characterize cell damage caused by vaginal Candida albicans isolates from women with different symptomatologies. It was evaluated 12 clinical isolates of C. albicans from vaginal samples: 4 from asymptomatic women (AS), 4 from women with a single episode of vulvovaginal candidiasis (VVC) and...

journal_title：Journal of medical microbiology

authors： Faria DR,Sakita KM,Akimoto-Gunther LS,Kioshima ÉS,Svidzinski TIE,Bonfim-Mendonça PS

更新日期：2017-08-01 00:00:00

abstract：:Erythrocyte-sensitising antigens (AE) were prepared from Vibrio cholerae serotypes, from EL-Tor vibrio, Escherichia coli and Salmonella enteritidis by digesting the organisms with NaOH followed by precipitation with alcohol. When AE was used in indirect haemagglutination (IHA) tests, the results in a number of cases w...

journal_title：Journal of medical microbiology

authors： Garabedian GA,Barsumian EL,Malakian A

更新日期：1975-02-01 00:00:00

abstract：:Microcirculation plays a crucial role in mucosal physiological function as well as repair of gastric mucosal damage. Endothelial cell damage is known to disturb microcirculation and suppress angiogenesis. Therefore, the direct effect of Helicobacter pylori on endothelial cells in vitro was investigated with H. pylori ...

journal_title：Journal of medical microbiology

authors： Kurosawa A,Miwa H,Hirose M,Tsune I,Nagahara A,Sato N

更新日期：2002-05-01 00:00:00

abstract：:Catalase (KatA) is known to play an important role in oxidative stress resistance in many bacterial species and a homologue exists in Helicobacter hepaticus, a member of the enterohepatic Helicobacter species. Here, a katA mutant was constructed by insertional mutagenesis and its oxidative stress phenotype was investi...

journal_title：Journal of medical microbiology

authors： Hong Y,Wang G,Maier RJ

更新日期：2007-04-01 00:00:00

abstract：:Experiments were performed to determine whether a modern flow cytometer could be used to study bacterial populations in suspension, with particular reference to their morphological characteristics and their responses to antibiotics. The FACScan, a commercial benchtop flow cytometer fitted with an air-cooled laser, des...

journal_title：Journal of medical microbiology

authors： Gant VA,Warnes G,Phillips I,Savidge GF

更新日期：1993-08-01 00:00:00

abstract：:A porous inorganic material (Anopore) was employed as a microbial culture and microcolony imaging support. Rapid Anopore-based antibiotic sensitivity testing (AST) methods were developed to assess the growth of clinical isolates, with the primary focus on testing the response of the Enterobacteriaceae to trimethoprim,...

journal_title：Journal of medical microbiology

authors： Ingham CJ,van den Ende M,Wever PC,Schneeberger PM

更新日期：2006-11-01 00:00:00

abstract：:The carriage on skin lesions, on healthy skin and in the nose, of micro-organisms that produce antibiotics active against Staphylococcus aureus has been studied. About 5% of sites (10% of all patients) yielded such organisms. No evidence could be found that the presence of producer organisms in skin lesions had a prot...

journal_title：Journal of medical microbiology

authors： Noble WC,Willie JA

更新日期：1980-05-01 00:00:00

abstract：:Community-associated meticillin-resistant Staphylococcus aureus (CA-MRSA) have emerged globally and have been associated with more severe disease than healthcare-associated MRSA (HA-MRSA). The purpose of this study was to determine whether laboratory measures of virulence can distinguish dominant CA-MRSA clones from H...

journal_title：Journal of medical microbiology

authors： Boakes E,Marbach H,Lynham S,Ward M,Edgeworth JD,Otter JA

更新日期：2016-12-01 00:00:00

abstract：:This study investigated the influence of the carbon source of the growth medium, strains of Candida albicans and source of epithelial cells, and the influence of smoking and gender, on the adhesion of C. albicans to epithelial cells from insulin-using diabetic patients. Adhesion was determined by an autologous adhesio...

journal_title：Journal of medical microbiology

authors： Willis AM,Coulter WA,Hayes JR,Bell P,Lamey PJ

更新日期：2000-03-01 00:00:00

abstract：:Over the last 30 years, Serratia marcescens has become an important cause of nosocomial infection. There have been many reports concerning the identification, antibiotic susceptibility, pathogenicity, epidemiological investigations and typing of this organism. Accurate identification is important in defining outbreaks...

journal_title：Journal of medical microbiology

authors： Hejazi A,Falkiner FR

更新日期：1997-11-01 00:00:00

abstract：:Biofilms are colonies of microbial cells encased in a self-produced organic polymeric matrix and represent a common mode of microbial growth. Microbes growing as biofilm are highly resistant to commonly used antimicrobial drugs. We aimed to screen and characterize biofilm formation by different isolates of Candida on ...

journal_title：Journal of medical microbiology

authors： Zahran KM,Agban MN,Ahmed SH,Hassan EA,Sabet MA

更新日期：2015-04-01 00:00:00

abstract：:A new 1,8-naphthyridine quinolone antimicrobial, PD131628, was found to be as, or more active than ciprofloxacin or ofloxacin against Escherichia coli and considerably more active than the two 4-quinolone agents against staphylococci in terms of both MIC and OBC (the optimum bactericidal concentration, at which the ra...

journal_title：Journal of medical microbiology

authors： Lewin CS

更新日期：1992-05-01 00:00:00

abstract：:The eubacterial population was studied in faecal samples of related and unrelated children. Temporal temperature gradient gel electrophoresis (TTGE) provided a snapshot of the bacterial population and allowed calculation of the degree of similarity in the predominant faecal microflora of identical twin pairs, fraterna...

journal_title：Journal of medical microbiology

authors： Stewart JA,Chadwick VS,Murray A

更新日期：2005-12-01 00:00:00

abstract：PURPOSE:Nodal may play an important role in the development of cancers. The present study was designed to determine the effects of Nodal induced by respiratory syncytial virus (RSV) infection on the occurrence and development of lung cancer and the underlying mechanisms. METHODOLOGY:After verification of RSV infection...

journal_title：Journal of medical microbiology

authors： Xiang Z,Liang Z,Yanfeng H,Leitao K

更新日期：2017-10-01 00:00:00

abstract：:The aim of the present study was to investigate the colonization status of Clostridium difficile in healthy individuals. In total, 139 healthy adults from two study groups were examined at intervals of 3 months. Among the 18 positive subjects, the number of subjects from whom C. difficile was isolated once, twice, thr...

journal_title：Journal of medical microbiology

authors： Ozaki E,Kato H,Kita H,Karasawa T,Maegawa T,Koino Y,Matsumoto K,Takada T,Nomoto K,Tanaka R,Nakamura S

更新日期：2004-02-01 00:00:00

abstract：:Multidrug-resistant organisms (MDROs) pose significant infection-control challenges in settings with high prevalence and limited isolation facilities. This observational study in an 800-bed hospital determined the prevalence, bacterial density and genetic relatedness of MDROs isolated from ward surfaces, medical devic...

journal_title：Journal of medical microbiology

authors： Tan TY,Tan JSM,Tay H,Chua GH,Ng LSY,Syahidah N

更新日期：2013-05-01 00:00:00

abstract：:This study investigated the comparative performance of the Amplicor assay and an in-house semi-automated, multiplex real-time PCR for the diagnosis of genital chlamydial infection. Four different assays, the COBAS Amplicor CT test (Amplicor PCR), in-house real-time PCR (IHRT-PCR), in-house nested cryptic plasmid PCR a...

journal_title：Journal of medical microbiology

authors： Jalal H,Al-Suwaine A,Stephen H,Carne C,Sonnex C

更新日期：2007-03-01 00:00:00

abstract：:Bacterial invasion of host epithelial cells plays an important role in the pathogenesis of periodontal diseases; however, the interactions between subgingival species and the gingival crevice cells are not fully understood. This study determined the prevalence of a group of oral bacterial species on or in epithelial c...

journal_title：Journal of medical microbiology

authors： Colombo AV,Silva CM,Haffajee A,Colombo APV

更新日期：2006-05-01 00:00:00

abstract：:The family of leptospiral immunoglobulin-like (lig) genes comprises ligA, ligB and ligC. This study used PCR to demonstrate the presence of lig genes among serovars from a collection of leptospiral strains and clinical isolates. Whilst ligA and ligC appeared to be present in a limited number of pathogenic serovars, th...

journal_title：Journal of medical microbiology

authors： Cerqueira GM,McBride AJA,Picardeau M,Ribeiro SG,Moreira ÂN,Morel V,Reis MG,Ko AI,Dellagostin OA

更新日期：2009-09-01 00:00:00

abstract：:A polymerase chain reaction (PCR) assay with oligonucleotide primers homologous to a portion of the urease C gene of Helicobacter pylori was evaluated for specificity with pure DNA and biopsy material. The assay was used to test for the presence of the organism in dental plaque. The species specificity of detection wa...

journal_title：Journal of medical microbiology

authors： Bickley J,Owen RJ,Fraser AG,Pounder RE

更新日期：1993-11-01 00:00:00