Successful substitution of fetal calf serum by human plasma for bulk cultivation of Leishmania donovani promastigotes.

abstract：:Nucleic acid amplification techniques (NAATs) have been demonstrated to make significant improvements in the diagnosis of tuberculosis (TB), particularly in the time to diagnosis and the diagnosis of smear-negative TB. The BD ProbeTec strand displacement amplification (SDA) system for the diagnosis of pulmonary and no...

journal_title：Journal of medical microbiology

authors： McHugh TD,Pope CF,Ling CL,Patel S,Billington OJ,Gosling RD,Lipman MC,Gillespie SH

更新日期：2004-12-01 00:00:00

abstract：:Detection of latent Mycobacterium tuberculosis is a challenge in the diagnosis of asymptomatic, subclinical tuberculosis. We report the development of an immunofluorescence technique to visualize and enumerate M. tuberculosis in latently infected rabbit lungs where no acid-fast-stained organisms were seen and no culti...

journal_title：Journal of medical microbiology

authors： Subbian S,Eugenin E,Kaplan G

更新日期：2014-11-01 00:00:00

abstract：:The obligate intracellular pathogen Anaplasma phagocytophilum is transmitted by ticks and causes human granulocytic anaplasmosis, tick-borne fever of ruminants, and equine and canine granulocytic anaplasmosis. In a previous study, the perilipin (PLIN) gene was identified as one of the genes differentially expressed in...

journal_title：Journal of medical microbiology

authors： Manzano-Roman R,Almazán C,Naranjo V,Bloui EF,Kocan KM,de la Fuente J

更新日期：2008-02-01 00:00:00

abstract：:The use of alkaline phosphatase fusion methodology to identify Helicobacter pylori exported proteins enabled the identification of an open reading frame (ORF) encoding a highly immunogenic, previously uncharacterised exported protein. The predicted aminoacid sequence displays a typical N-terminal signal peptide and co...

journal_title：Journal of medical microbiology

authors： Oliaro J,Johnson RD,Chen W,Chadwick VS,Murray A

更新日期：2000-07-01 00:00:00

abstract：:The object of this study was to develop and characterize experimental Burkholderia mallei aerosol infection in BALB/c mice. Sixty-five mice were infected with 5000 [approx. 2.5 median lethal doses (MLD)] B. mallei strain ATCC 23344(T) bacteria by the aerosol route. Bacterial counts within lung, liver, spleen, brain, k...

journal_title：Journal of medical microbiology

authors： Lever MS,Nelson M,Ireland PI,Stagg AJ,Beedham RJ,Hall GA,Knight G,Titball RW

更新日期：2003-12-01 00:00:00

abstract：:Francisella tularensis is a highly virulent intracellular bacterium capable of rapid multiplication in phagocytic cells. Previous studies have revealed that activation of F. tularensis-infected macrophages leads to control of infection and reactive nitrogen and oxygen species make important contributions to the bacter...

journal_title：Journal of medical microbiology

authors： Tancred L,Telepnev MV,Golovliov I,Andersson B,Andersson H,Lindgren H,Sjöstedt A

更新日期：2011-11-01 00:00:00

abstract：:Burkholderia pseudomallei is isolated frequently from the soil in regions where the disease melioidosis occurs. However, recent surveys in Thailand have shown that the frequency of isolation of the organism from soil samples is not directly related to the incidence of melioidosis in an area. To determine whether strai...

journal_title：Journal of medical microbiology

authors： Trakulsomboon S,Dance DA,Smith MD,White NJ,Pitt TL

更新日期：1997-07-01 00:00:00

abstract：:Urinary isolates of Escherichia coli that were resistant to trimethoprim were collected in Glasgow Royal Infirmary during 1979 and 1980. Eighty-eight were resistant to trimethoprim 1024 micrograms/ml and 80 (92%) were also resistant to sulphamethoxazole 1024 micrograms/ml; 73% were multiresistant. Plasmids were detect...

journal_title：Journal of medical microbiology

authors： Kraft CA,Platt DJ,Timbury MC

更新日期：1983-11-01 00:00:00

abstract：:We report what we believe is the first reported case of Streptococcus mutans endocarditis complicated by vertebral discitis. The case is particularly interesting and topical as it occurred in a patient with pre-existing cardiac valvular disease who had recently had a dental procedure without antibiotic prophylaxis fol...

journal_title：Journal of medical microbiology

authors： Biswas S,Bowler ICJW,Bunch C,Prendergast B,Webster DP

更新日期：2010-10-01 00:00:00

abstract：:Lactulose exerts a beneficial effect on hepatic encephalopathy by decreasing toxic short-chain (iC4-nC6) fatty acid (isobutyrate, butyrate, isovalerate, valerate, isocaproate and caproate) production. However, the precise mechanism by which lactulose exerts this effect remains uncertain. This study investigated the ef...

journal_title：Journal of medical microbiology

authors： Ito Y,Moriwaki H,Muto Y,Kato N,Watanabe K,Ueno K

更新日期：1997-01-01 00:00:00

abstract：:Effects of four mucopolysaccharides and dextran sulphate on penicillin-induced lysis of Staphylococcus aureus FDA 209P were studied. Heparin and dextran sulphate inhibited lysis, whereas hyaluronic acid enhanced it. Chondroitin sulphates A and C had no effect. Incubation of S. aureus suspended in 0.03 M phosphate buff...

journal_title：Journal of medical microbiology

authors： Kiriyama T,Miyake Y,Sugai M,Kobayashi K,Yoshiga K,Takada K,Suginaka H

更新日期：1987-12-01 00:00:00

abstract：:In order to develop a model for the study of gastric spiral bacteria, and based on the observation that Wistar rats do not carry urease-positive spiral bacteria in their gastric mucosa, mucus from a pig naturally colonised by 'Gastrospirillum suis' (an organism with I6S rDNA 99.5% similar to that of 'G. hominis' type ...

journal_title：Journal of medical microbiology

authors： Mendes EN,Queiroz DM,Coimbra RS,Moura SB,Barbosa AJ,Rocha GA

更新日期：1996-02-01 00:00:00

abstract：:Intestinal absorption of arbutin (p-hydroxyphenyl-beta-glucoside), a non-metabolised analogue of d-glucose, and net flux of water out of the small intestinal lumen were found to be decreased in vitro in rats fed with Candida albicans. In rats on a protein-deficient diet and fed C. albicans there was net secretion of w...

journal_title：Journal of medical microbiology

authors： Burke V,Gracey M

更新日期：1980-02-01 00:00:00

abstract：:The mcr-1 gene encodes a phosphoethanolamine transferase, which confers resistance to colistin by transferring phosphoethanolamine to lipid A. This study describes the emergence of a colistin- and carbapenem-resistant clinical isolate of Escherichia coli harbouring mcr-1 and blaNDM-5 genes, located on 90 and 150 Kb pl...

journal_title：Journal of medical microbiology

authors： Uchida H,Tada T,Sugahara Y,Kato A,Miyairi I,Kirikae T

更新日期：2018-08-01 00:00:00

abstract：PURPOSE:The aim of this study was to assess the biotope of the Cryptococcus neoformans/Cryptococcus gattii species complex from Ivory Coast, and clarify the possible epidemiological relationship between environmental and clinical strains. METHODOLOGY:Samples from Eucalyptus camaldulensis (n=136), Mangifera indica (n=1...

journal_title：Journal of medical microbiology

authors： Kassi FK,Bellet V,Drakulovski P,Krasteva D,Roger F,Valérie BA,Aboubakar T,Doumbia A,Kouakou GA,Delaporte E,Reynes J,Yavo W,Menan HIE,Bertout S

更新日期：2018-01-01 00:00:00

abstract：:Nasal polyp cells in primary culture from cystic fibrosis (CF) and non-CF patients were compared for the ability to bind Pseudomonas aeruginosa cells and for the presence of sulphated glycoconjugates at the epithelial cell surface. Quantitation of bacterial adhesion, by scanning electronmicroscopy, showed no significa...

journal_title：Journal of medical microbiology

authors： Plotkowski MC,Chevillard M,Pierrot D,Altemayer D,Puchelle E

更新日期：1992-02-01 00:00:00

abstract：:Pulsed-field gel electrophoresis typing was performed on a retrospective set of 129 Moraxella catarrhalis isolates obtained over a 20 month period from 70 children admitted to, or presenting at, the Erasmus University Medical Center, Rotterdam, The Netherlands. The mean age of the children (at the end of the study) wa...

journal_title：Journal of medical microbiology

authors： Hays JP,Eadie K,Verduin CM,Hazelzet J,Verbrugh H,van Belkum A

更新日期：2003-09-01 00:00:00

abstract：:Rickettsia conorii is endemic in Greece, though only a few cases of infection have been published to date. The case of a 58-year-old man from northern Greece with a severe form of Mediterranean spotted fever and rapid neurological deterioration is presented here. The patient received antibiotic treatment with doxycycl...

journal_title：Journal of medical microbiology

authors： Tzavella K,Hatzizisis IS,Vakali A,Mandraveli K,Zioutas D,Alexiou-Daniel S

更新日期：2006-03-01 00:00:00

abstract：:A study was undertaken to quantify the expression of prostaglandin (PG) receptors and find the effect of gestational age on expression of PG receptor genes in Chlamydia trachomatis-infected recurrent spontaneous aborters (RSA). Endometrial curettage tissue (ECT) was collected from 130 RSA (Group I) and 100 age-matched...

journal_title：Journal of medical microbiology

authors： Singh N,Prasad P,Singh LC,Das B,Rastogi S

更新日期：2016-06-01 00:00:00

abstract：:Alpha protoxin of Staphylococcus aureus "Wood 46" was activated by trypsin which had been coupled to carboxymethylcellulose, as indicated by the toxin's ability to hydrolyse tosyl-arginine methylester (TAME). A Lineweaver-Burk plot of the degradation of TAME by toxin and trypsin showed that toxin had a greater affinit...

journal_title：Journal of medical microbiology

authors： Wiseman GM,Caird JD,Fackrell HB

更新日期：1975-02-01 00:00:00

abstract：:The relationship between Streptococcus constellatus, one of the species of the "Streptococcus milleri group", and Prevotella intermedia was studied in a model of pneumonia in mice and in vitro to elucidate mechanisms of pathogenicity in "S. milleri group"-associated pulmonary infection. Acute pneumonia with or without...

journal_title：Journal of medical microbiology

authors： Shinzato T,Saito A

更新日期：1994-02-01 00:00:00

abstract：:The toxic effects of the haemorrhagic toxin of Clostridium sporogenes were studied in mice, rats, guinea-pigs and rabbits, and in various cultured cells. In rabbits, but not in the other animals, intradermal injection with crude toxin and its injection into a ligated intestinal loop caused haemorrhage in both the skin...

journal_title：Journal of medical microbiology

authors： Hara-Kudo Y,Ogura A,Noguchi Y,Kumagai S

更新日期：1997-04-01 00:00:00

abstract：:A commercial method (RAPIDEC ur; bioMérieux UK Ltd, Basingstoke) and an in-house scheme based on conventional tests were compared for the identification of 179 commonly encountered gram-negative urinary pathogens. The methods identified correctly to genus level 97% and 84% of organisms, respectively. RAPIDEC ur was su...

journal_title：Journal of medical microbiology

authors： Batchelor BI

更新日期：1995-07-01 00:00:00

abstract：:Leclercia adecarboxylata is a rarely reported human pathogen, most commonly affecting immunocompromised individuals. In reported cases of immunocompetent patients infected with this organism, it is seen exclusively in the context of polymicrobial infections. We report here the case of an abscess in an immunocompetent ...

journal_title：Journal of medical microbiology

authors： Hess B,Burchett A,Huntington MK

更新日期：2008-07-01 00:00:00

abstract：PURPOSE:We previously identified an association between CC22 meticillin-resistant Staphylococcus aureus (MRSA) bloodstream infection isolates with an elevated vancomycin MIC (V-MIC) in the susceptible range (1.5-2 mg l-1) and endocarditis. This study explores whether these isolates have a specific phenotype consistent ...

journal_title：Journal of medical microbiology

authors： Marbach H,Boakes E,Lynham S,Ward M,Otter JA,Edgeworth JD

更新日期：2017-05-01 00:00:00

abstract：PURPOSE:An observational study was performed to investigate the carriage rate and serotypes of Streptococcus pneumoniae in the 13-valent pneumococcal conjugate vaccine (PCV13) era in Taiwan. METHODOLOGY:From March 2014 to March 2015 a total of 500 healthy children and their households (631 adults) were enrolled from t...

journal_title：Journal of medical microbiology

authors： Janapatla RP,Su LH,Chen HH,Chang HJ,Tsai TC,Chen PY,Chen CL,Chiu CH

更新日期：2017-06-01 00:00:00

abstract：:Monoclonal antibodies (MAbs) N-59 and RU-1 were produced by immunisation of mice with streptokinase secreted by Streptococcus group A, type 12, strain A374 isolated from a patient with post-streptococcal glomerulonephritis (PSGN) and were characterised by Western blot analysis. MAb N-59 recognised antigenic determinan...

journal_title：Journal of medical microbiology

authors： Ohkuni H,Todome Y,Yoshimura K,Yamamoto T,Suzuki H,Yokomuro K,Johnston KH,Zabriskie JB

更新日期：1991-07-01 00:00:00

abstract：:Aspiration of oropharyngeal bacteria and fungi is occasionally suspected in patients with pneumonia. A patient with oral carcinoma underwent chemoradioimmunotherapy and, about 4 weeks from the start of the therapy, the patient suffered from severe oral mucositis induced by chemoradiotherapy, and candidal pneumonia was...

journal_title：Journal of medical microbiology

authors： Yamamoto T,Ueta E,Kamatani T,Osaki T

更新日期：2005-05-01 00:00:00

abstract：:The prevalence of community-acquired meticillin-resistant Staphylococcus aureus (CA-MRSA) strains has become a serious problem worldwide. The aim of this study was to investigate the annual transitions of MRSA strains with the CA-MRSA feature, which were identified as SCCmec type IV or V, in a hospital setting in Japa...

journal_title：Journal of medical microbiology

authors： Ito A,Nakaminami H,Fujii T,Utsumi K,Noguchi N

更新日期：2015-07-01 00:00:00

abstract：:Diagnosis of human immunodeficiency virus (HIV) infection by antibody-based testing allows for some recently infected individuals to be falsely assessed as non-infected. Since such individuals often have high viral loads and are capable of transmitting HIV, it is an imperative public health need to identify these indi...

journal_title：Journal of medical microbiology

authors： Ren A,Louie B,Rauch L,Castro L,Liska S,Klausner JD,Pandori MW

更新日期：2008-10-01 00:00:00