Identification of an immunogenic 18-kDa protein of Helicobacter pylori by alkaline phosphatase gene fusions.

abstract：:Rapid identification of the causative bacteria of sepsis in patients can contribute to the selection of appropriate antibiotics and improvement of patients' prognosis. Genotypic identification is an emerging technology that may provide an alternative method to, or complement, established phenotypic identification proc...

journal_title：Journal of medical microbiology

authors： Motoshima M,Yanagihara K,Morinaga Y,Matsuda J,Hasegawa H,Kohno S,Kamihira S

更新日期：2012-11-01 00:00:00

abstract：:Klebsiella pneumoniae meningitis has recently become an increasingly common cause of central nervous system infection. The invasion of bacteria within the subarachnoid space stimulates the release of pro-inflammatory cytokines and chemokines, triggering a host immune response. The aim of the present study was to evalu...

journal_title：Journal of medical microbiology

authors： Barichello T,Simões LR,Valvassori SS,Generoso JS,Aveline PEDV,Dominguini D,Elias SG,Vilela MC,Quevedo J,Teixeira AL

更新日期：2014-01-01 00:00:00

abstract：:The gingival sulcus is the shallow crevice around the tooth, and its epithelium is a gateway for initial bacterial infection in periodontal disease. Recent studies have shown that Actinobacillus actinomycetemcomitans invades an epithelial cell line, KB cells, in vitro. The aim of the present study was to clarify the c...

journal_title：Journal of medical microbiology

authors： Kato S,Nakashima K,Inoue M,Tomioka J,Nonaka K,Nishihara T,Kowashi Y

更新日期：2000-08-01 00:00:00

abstract：PURPOSE:Strains of type emm89 Streptococcus pyogenes have recently increased in frequency as a cause of human infections in several countries in Europe and North America. This increase has been molecular epidemiologically linked with the emergence of a new genetically distinct clone, designated clade 3. We sought to ex...

journal_title：Journal of medical microbiology

authors： Beres SB,Olsen RJ,Ojeda Saavedra M,Ure R,Reynolds A,Lindsay DSJ,Smith AJ,Musser JM

更新日期：2017-12-01 00:00:00

abstract：:Conventional culture and drug susceptibility testing (DST) methods for Mycobacterium tuberculosis are laborious and time consuming. For this reason alternative rapid culture and DST techniques are urgently needed to shorten the time for drug-resistance detection. A total of 222 smear-positive sputum samples were evalu...

journal_title：Journal of medical microbiology

authors： Imperiale BR,Morcillo NS,Palomino JC,Vandamme P,Martin A

更新日期：2014-04-01 00:00:00

abstract：:The production of toxins A and B by Clostridium difficile was greatly enhanced under biotin-limited conditions, in which a 140-kDa protein was expressed strongly. Gene cloning revealed that this protein was a homologue of formylglycinamidine ribonucleotide synthetase (FGAM synthetase, EC 6.3.5.3), which is known as Pu...

journal_title：Journal of medical microbiology

authors： Maegawa T,Karasawa T,Ohta T,Wang X,Kato H,Hayashi H,Nakamura S

更新日期：2002-01-01 00:00:00

abstract：:The recently-introduced semi-automated bacterial identification system, the Quantum II BID, is designed to identify fermentative and non-fermentative gram-negative bacilli in 4-5 h. The system was evaluated independently by the two participating laboratories. Inter-laboratory reproducibility was determined by testing ...

journal_title：Journal of medical microbiology

authors： Stevens M,Henrichsen C,Smith M

更新日期：1986-03-01 00:00:00

abstract：:Antigen participating in cell-mediated immunity was isolated from a sonic extract of Mycobacterium tuberculosis H37Rv. The purified antigen had a mol. wt 150 000-200 000, based upon its Rf in 7.5% polyacrylamide gel in the presence of sodium dodecyl sulphate. In simple agarose gel electrophoresis it had an Rf of 0.85....

journal_title：Journal of medical microbiology

authors： Hattikudur S,Kamat RS

更新日期：1984-08-01 00:00:00

abstract：:The surface structures of the cell envelopes of 16 clinical isolates of Pseudomonas aeruginosa were examined by electronmicroscopy with the new fixation technique of freeze-substitution. Two types of structures were observed among the organisms. In one group of strains, mostly isolated from blood, a dense fibrous laye...

journal_title：Journal of medical microbiology

authors： Misumi H,Umeda A,Eguchi K,Okada K,Sawae Y,Niho Y,Amako K

更新日期：1994-04-01 00:00:00

abstract：PURPOSE:Membrane fluidity to a large extent is governed by the presence of branched-chain fatty acids (BCFAs). Branched-chain α-keto acid dehydrogenase (BKD) is the key enzyme in BCFA synthesis. A Staphylococcus aureus BKD-deficient strain still produced substantial levels of BCFAs. Pyruvate dehydrogenase (PDH) with st...

journal_title：Journal of medical microbiology

authors： Singh VK,Sirobhushanam S,Ring RP,Singh S,Gatto C,Wilkinson BJ

更新日期：2018-04-01 00:00:00

abstract：:In 2002, a Scottish bat conservationist developed a rabies-like disease and subsequently died. This was caused by infection with European bat lyssavirus 2 (EBLV-2), a virus closely related to Rabies virus (RABV). The source of this infection and the means of transmission have not yet been confirmed. In this study, the...

journal_title：Journal of medical microbiology

authors： Johnson N,Phillpotts R,Fooks AR

更新日期：2006-06-01 00:00:00

abstract：:The Gen-Probe APTIMA Combo 2 assay has previously been reported to have a high sensitivity. The end point of this assay was evaluated using highly purified chlamydial elementary bodies (EBs). The performance of the APTIMA Combo 2 assay was compared with a commercially available PCR kit, AMPLICOR Chlamydia trachomatis....

journal_title：Journal of medical microbiology

authors： Ikeda-Dantsuji Y,Konomi I,Nagayama A

更新日期：2005-04-01 00:00:00

abstract：PURPOSE:Meticillin-resistant Staphylococcus aureus (MRSA) biofilm formation in humans is of serious clinical concern. Previous in vitro studies have been performed with biofilms grown only on inorganic substrates; therefore, we investigated the vancomycin (VCM) resistance of MRSA biofilms grown on skin tissue. METHODO...

journal_title：Journal of medical microbiology

authors： Jimi S,Miyazaki M,Takata T,Ohjimi H,Akita S,Hara S

更新日期：2017-04-01 00:00:00

abstract：:Helicobacter pylori infection represents a key factor in the aetiology of various gastrointestinal diseases. H. pylori infection diagnosis is generally achieved using both invasive (e.g. biopsy of the gastric epithelium) and non-invasive methods. Therefore, cultivation on a growth medium becomes complex. Trypsin is a ...

journal_title：Journal of medical microbiology

authors： Peretz A,Paritsky M,Pastukh N,Koifman A,Brodsky D,Glyatman T,On A

更新日期：2015-06-01 00:00:00

abstract：:A porous inorganic material (Anopore) was employed as a microbial culture and microcolony imaging support. Rapid Anopore-based antibiotic sensitivity testing (AST) methods were developed to assess the growth of clinical isolates, with the primary focus on testing the response of the Enterobacteriaceae to trimethoprim,...

journal_title：Journal of medical microbiology

authors： Ingham CJ,van den Ende M,Wever PC,Schneeberger PM

更新日期：2006-11-01 00:00:00

abstract：:A collection of 72 strains of catalase-negative gram-positive, -negative and -variable cocco-bacilli isolated from samples of vaginal discharge from women with non-specific vaginal infection was examined in an attempt to develop an identification system for Gardnerella vaginalis that could be used in a diagnostic labo...

journal_title：Journal of medical microbiology

authors： Taylor E,Phillips I

更新日期：1983-02-01 00:00:00

abstract：:A 29-year-old pregnant woman was admitted to hospital with signs of sepsis and threatened pre-term labour. The premature neonate also showed signs of sepsis. Haemophilus influenzae biotype III was cultured from a midstream urine sample taken from the mother, maternal placental swabs and neonatal blood cultures. The pl...

journal_title：Journal of medical microbiology

authors： Warren S,Tristram S,Bradbury RS

更新日期：2010-03-01 00:00:00

abstract：:This study investigated the influence of the carbon source of the growth medium, strains of Candida albicans and source of epithelial cells, and the influence of smoking and gender, on the adhesion of C. albicans to epithelial cells from insulin-using diabetic patients. Adhesion was determined by an autologous adhesio...

journal_title：Journal of medical microbiology

authors： Willis AM,Coulter WA,Hayes JR,Bell P,Lamey PJ

更新日期：2000-03-01 00:00:00

abstract：:Intratracheal administration of purified Pseudomonas aeruginosa exoenzyme S elicited extensive, grossly observable damage in the rat lung within 2 h. Light and electronmicroscopy revealed injury and necrosis of bronchial epithelium, type I pneumocytes and capillary endothelial cells after 1 h; associated haemorrhage, ...

journal_title：Journal of medical microbiology

authors： Woods DE,Hwang WS,Shahrabadi MS,Que JU

更新日期：1988-06-01 00:00:00

abstract：PURPOSE:Listeria monocytogenes is a genetically heterogeneous species, which is divided into evolutionary lineages and clonal complexes (CCs). Not all L. monocytogenes isolates are equally likely to cause disease, with CC1, and in particular sequence type (ST) 1, being the most prevalent complex in human and ruminant i...

journal_title：Journal of medical microbiology

authors： Rupp S,Bärtschi M,Frey J,Oevermann A

更新日期：2017-07-01 00:00:00

abstract：:The rapid identification of pathogenic yeasts is a crucial step in ensuring that effective antifungal treatment is started as early as possible. CandiSelect 4 (CS4; Bio-Rad) is a new chromogenic medium for the isolation of fungi, the direct identification of Candida albicans and the presumptive identification of the m...

journal_title：Journal of medical microbiology

authors： Sendid B,François N,Standaert A,Dehecq E,Zerimech F,Camus D,Poulain D

更新日期：2007-04-01 00:00:00

abstract：:Tuberculosis is a major public health problem in India and other developing countries and has formed a lethal partnership with AIDS. It often presents a diagnostic challenge especially when clinical presentation is suggestive but bacteriological proof is lacking. The objective of this study was to compare the various ...

journal_title：Journal of medical microbiology

authors： Pahwa R,Hedau S,Jain S,Jain N,Arora VM,Kumar N,Das BC

更新日期：2005-09-01 00:00:00

abstract：:Two cases of severe community-acquired pneumococcal pneumonia (CAP) admitted to our hospital within 24 h are described, both in young males. These two cases illustrate the usefulness of the British Thoracic Society severity criteria and serve to emphasize the importance of early recognition of adverse physiology in cr...

journal_title：Journal of medical microbiology

authors： Kelly MG,Sharkey RA,Moles KW,Daly JG

更新日期：2004-01-01 00:00:00

abstract：:The purpose of this study was to verify whether the presence of any of the Helicobacter pylori cagPAI genes or segments--cagA, cagA promoter, cagE, cagM, tnpB, tnpA, cagT and the left end of the cag II (LEC) region--would be a useful marker for the risk of peptic ulcer disease development. H. pylori DNA extracted from...

journal_title：Journal of medical microbiology

authors： Mattar R,Marques SB,Monteiro MDS,Dos Santos AF,Iriya K,Carrilho FJ

更新日期：2007-01-01 00:00:00

abstract：:In recent years, hospitals in southeastern Europe have faced dramatically high rates of antibiotic-resistant Acinetobacter baumannii. We analysed the evolution of resistance among clinical isolates of A. baumannii group obtained from nine tertiary hospitals throughout Greece over 6 years (2010-2015). Identification an...

journal_title：Journal of medical microbiology

authors： Dafopoulou K,Tsakris A,Pournaras S

更新日期：2018-04-01 00:00:00

abstract：:Intrapartum chemoprophylaxis for pregnant group B streptococcus (GBS) carriers reduces vertical transmission, with a resultant decrease in neonatal as well as maternal morbidity from invasive GBS infection. Current Australian guidelines recommend penicillin for intrapartum prophylaxis of GBS carriers, with erythromyci...

journal_title：Journal of medical microbiology

authors： Garland SM,Cottrill E,Markowski L,Pearce C,Clifford V,Ndisang D,Kelly N,Daley AJ

更新日期：2011-02-01 00:00:00

abstract：PURPOSE:The current study has been designed to delineate the efficacy of geraniol (GE) on biofilm formation in Staphylococcus epidermidis as well as the effect of subinhibitory concentrations of GE on the development of adaptive resistance. METHODOLOGY:Biofilm biomass quantification assay was performed to evaluate the...

journal_title：Journal of medical microbiology

authors： Kannappan A,Sivaranjani M,Srinivasan R,Rathna J,Pandian SK,Ravi AV

更新日期：2017-10-01 00:00:00

abstract：:A solid-phase radioimmunoassay was used to detect IgG antibodies against herpes-simplex virus antigens (capsid, envelope and excreted) and against measles virus antigen in serum and cerebrospinal fluid (CSF) specimens of 61 patients with no evidence of infectious or demyelinating disease of the central nervous system....

journal_title：Journal of medical microbiology

authors： Kalimo KO,Marttila RJ,Ziola BR,Matikainen MT,Panelius M

更新日期：1977-11-01 00:00:00

abstract：:A novel drug delivery system for osteomyelitis was developed in which a porous hydroxyapatite block (HAB) is loaded with antibiotic by a centrifugation method. In this study, implantation of HABs loaded with the aminoglycoside antibiotic, arbekacin, were tested in established Staphylococcus aureus osteomyelitis in the...

journal_title：Journal of medical microbiology

authors： Itokazu M,Ohno T,Tanemori T,Wada E,Kato N,Watanabe K

更新日期：1997-09-01 00:00:00

abstract：:Streptococcus pneumoniae remains a leading cause of serious paediatric disease. However, there are few published epidemiological data regarding invasive pneumococcal disease (IPD) in many countries in South East Asia, including Singapore. Baseline data for IPD are essential to inform policy regarding pneumococcal conj...

journal_title：Journal of medical microbiology

authors： Jefferies JMC,Tee WSN,Clarke SC

更新日期：2011-06-01 00:00:00