Distribution and transferability of plasmids in trimethoprim resistant urinary Escherichia coli.

Abstract:

:Urinary isolates of Escherichia coli that were resistant to trimethoprim were collected in Glasgow Royal Infirmary during 1979 and 1980. Eighty-eight were resistant to trimethoprim 1024 micrograms/ml and 80 (92%) were also resistant to sulphamethoxazole 1024 micrograms/ml; 73% were multiresistant. Plasmids were detected in 98% of strains and 60% carried two or more. Half of the isolates transferred trimethoprim resistance to E. coli K12 and 70% of these cotransferred resistance to sulphonamide although these markers were often not linked. Trimethoprim resistance was carried on 12 different plasmids, four of which also conferred sulphonamide resistance. All except two carried streptomycin resistance which suggests that Tn7 was probably present. The results are discussed in relation to current prescribing policy.

journal_name

J Med Microbiol

authors

Kraft CA,Platt DJ,Timbury MC

doi

10.1099/00222615-16-4-433

subject

Has Abstract

pub_date

1983-11-01 00:00:00

pages

433-41

issue

4

eissn

0022-2615

issn

1473-5644

journal_volume

16

pub_type

杂志文章
  • Characterisation of Eubacterium-like strains isolated from oral infections.

    abstract::The genus Eubacterium currently includes a heterogeneous group of gram-positive, non-spore-forming anaerobic bacilli, many of which are slow growing, fastidious and generally unreactive in biochemical tests. As a consequence, cultivation and identification of isolates are difficult and the taxonomy of the group remain...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-50-11-947

    authors: Downes J,Munson MA,Spratt DA,Kononen E,Tarkka E,Jousimies-Somer H,Wade WG

    更新日期:2001-11-01 00:00:00

  • Contact-haemolysin production by entero-invasive Escherichia coli and shigellae.

    abstract::Entero-invasive Escherichia coli (EIEC) and shigellae were tested for contact-haemolysin (CH) with red blood cells (RBCs) of guinea-pig, rabbit, rat, mouse, monkey, man, sheep and chicken; all bacteria showed the best lysis with guinea-pig RBCs. The best culture medium for CH activity of shigellae was tryptic soy brot...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-35-6-330

    authors: Haider K,Albert MJ,Hossain A,Nahar S

    更新日期:1991-12-01 00:00:00

  • Mycotic aneurysm of the abdominal aorta caused by Haemophilus influenzae type f.

    abstract::Prior to the advent of the Haemophilus influenzae type b vaccine, invasive infections due to H. influenzae type f were rarely described. However, the epidemiology of H. influenzae is changing. While the incidence of invasive infections due to H. influenzae is declining in children, such infections are becoming more co...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.055228-0

    authors: Suarez CJ,Glover WA,Cowan J,Smith A,Clarridge JE

    更新日期:2013-04-01 00:00:00

  • Experimental aerogenic Burkholderia mallei (glanders) infection in the BALB/c mouse.

    abstract::The object of this study was to develop and characterize experimental Burkholderia mallei aerosol infection in BALB/c mice. Sixty-five mice were infected with 5000 [approx. 2.5 median lethal doses (MLD)] B. mallei strain ATCC 23344(T) bacteria by the aerosol route. Bacterial counts within lung, liver, spleen, brain, k...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.05180-0

    authors: Lever MS,Nelson M,Ireland PI,Stagg AJ,Beedham RJ,Hall GA,Knight G,Titball RW

    更新日期:2003-12-01 00:00:00

  • A paradigm for the molecular identification of Mycobacterium species in a routine diagnostic laboratory.

    abstract::The aim of this study was to improve the identification of Mycobacterium species in the context of a UK teaching hospital. Real-time PCR assays were established to enable the rapid differentiation between Mycobacterium tuberculosis (MTB) complex and Mycobacterium species other than tuberculosis (MOTT), followed by 16S...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.46855-0

    authors: Williams KJ,Ling CL,Jenkins C,Gillespie SH,McHugh TD

    更新日期:2007-05-01 00:00:00

  • Prevalence of Vibrio cholerae with heat-stable enterotoxin (NAG-ST) and cholera toxin genes; restriction fragment length polymorphisms of NAG-ST genes among V. cholerae O serogroups from a major shrimp production area in Thailand.

    abstract::A total of 148 Vibrio cholerae isolates from a major shrimp production area in Southern Thailand were examined by colony hybridisation for genes encoding heat-stable enterotoxin (NAG-ST) and cholera toxin (CT). Only non-O1 V. cholerae strains were found to harbour NAG-ST (14 of 146) whereas no strains hybridised with ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-43-3-216

    authors: Dalsgaard A,Serichantalergs O,Shimada T,Sethabutr O,Echeverria P

    更新日期:1995-09-01 00:00:00

  • Rapid antibiotic sensitivity testing and trimethoprim-mediated filamentation of clinical isolates of the Enterobacteriaceae assayed on a novel porous culture support.

    abstract::A porous inorganic material (Anopore) was employed as a microbial culture and microcolony imaging support. Rapid Anopore-based antibiotic sensitivity testing (AST) methods were developed to assess the growth of clinical isolates, with the primary focus on testing the response of the Enterobacteriaceae to trimethoprim,...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.46585-0

    authors: Ingham CJ,van den Ende M,Wever PC,Schneeberger PM

    更新日期:2006-11-01 00:00:00

  • Heterogeneity within apparently pure cultures of Escherichia coli freshly isolated from significant bacteriuria.

    abstract::Pyrolysis mass spectrometry (PyMS), a highly discriminatory method for comparison of isolates, was used to assess the homogeneity of colonies taken from apparently pure cultures of Escherichia coli in 10 urine specimens. Ten randomly selected colonies were subcultured from each urine for comparison. For six urines, th...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-45-5-349

    authors: Freeman R,Sisson PR,Burdess D

    更新日期:1996-11-01 00:00:00

  • Predictive value of direct nitrate reductase assay and its clinical performance in the detection of multi- and extensively drug-resistant tuberculosis.

    abstract::Conventional culture and drug susceptibility testing (DST) methods for Mycobacterium tuberculosis are laborious and time consuming. For this reason alternative rapid culture and DST techniques are urgently needed to shorten the time for drug-resistance detection. A total of 222 smear-positive sputum samples were evalu...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.070219-0

    authors: Imperiale BR,Morcillo NS,Palomino JC,Vandamme P,Martin A

    更新日期:2014-04-01 00:00:00

  • Radiometric studies on the use of selective inhibitors in the identification of Mycobacterium spp.

    abstract::Radiometric selective inhibition tests were developed and evaluated for the rapid differentiation of Mycobacterium spp. Both a p-nitrobenzoic acid (PNB) test and a commercially-prepared p-nitro-alpha-acetylamino-beta-hydroxypropiophenone (NAP) test successfully differentiated M. tuberculosis and M. bovis from "atypica...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-30-3-175

    authors: Collins T,Levett PN

    更新日期:1989-11-01 00:00:00

  • Comparative genome and evolution analysis of the locus of enterocyte effacement from enteropathogenic Escherichia coli Deng and its transcriptional response to ciprofloxacin.

    abstract:PURPOSE:In this study, we aimed to investigate the genomic characteristics and evolution of pathogenicity islands of an enteropathogenic Escherichia coli (EPEC) strain, and to obtain a transcriptional profile of EPEC under different concentrations of ciprofloxacin using microarray analysis. METHODOLOGY:The complete EP...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000790

    authors: Chen Z,Cheng H,Pan W,Zheng J,Li D,Lin F,Yu Z,Deng Q

    更新日期:2018-09-01 00:00:00

  • The value of LYM-1 cells for examining vacuole formation and loss of cell viability induced by culture supernates of Helicobacter pylori.

    abstract::Some strains of Helicobacter pylori are known to produce an extracellular cytotoxin that causes vacuolation in cultured mammalian cells. Screening for such strains makes use of HeLa cells which may not be sensitive enough to detect minimal changes. The aim of this study was to develop a more sensitive cell line. Vacuo...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-46-8-705

    authors: Wang XM,Kojima T,Satoh K,Taniguchi Y,Tokumaru K,Saifuku K,Seki M,Kihira K,Ido K,Uchida JY,Ohmori C,Takaoka T,Kimura K

    更新日期:1997-08-01 00:00:00

  • Biochemical characteristics, enterotoxigenicity and susceptibility to antimicrobial agents of clinical isolates of Aeromonas species encountered in the western region of Saudi Arabia.

    abstract::The biochemical characteristics, enterotoxigenicity and susceptibility to antibiotics are reported for 22 strains of Aeromonas species isolated from clinical specimens in the Western Region of Saudi Arabia. Aeromonas caviae was the species most frequently observed; a high proportion of these strains fermented lactose,...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-22-1-51

    authors: Gosling PJ

    更新日期:1986-08-01 00:00:00

  • A simple blood-culture bacterial pellet preparation for faster accurate direct bacterial identification and antibiotic susceptibility testing with the VITEK 2 system.

    abstract::An ammonium chloride procedure was used to prepare a bacterial pellet from positive blood cultures, which was used for direct inoculation of VITEK 2 cards. Correct identification reached 99% for Enterobacteriaceae and 74% for staphylococci. For antibiotic susceptibility testing, very major and major errors were 0.1 an...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.049361-0

    authors: Prod'hom G,Durussel C,Greub G

    更新日期:2013-05-01 00:00:00

  • Comparative growth of Bacteroides species in various anaerobic culture media.

    abstract::The growth of five species of Bacteroides in four anaerobic culture media was continuously monitored turbidimetrically. Interspecies differences were observed in the growth of Bacteroides spp. in the various media, but growth in Brain Heart Infusion broth supplemented with yeast extract, haemin and menadione, was cons...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-19-2-195

    authors: Eley A,Greenwood D,O'Grady F

    更新日期:1985-04-01 00:00:00

  • Mapping the genetic diversity within major clonal complexes of meticillin-resistant Staphylococcus aureus utilizing genome-wide fluorescent amplified fragment length polymorphism markers.

    abstract::The genetic diversity between major meticillin-resistant Staphylococcus aureus (MRSA) lineages was probed using fluorescent amplified fragment length polymorphism (FAFLP) as a random genome sampling tool. Genomic DNA was digested with endonucleases BglII and Csp6I and a subset of the restricted fragments were amplifie...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.049429-0

    authors: Desai D,Ellington MJ,Arnold C,Desai M

    更新日期:2012-12-01 00:00:00

  • Acquisition of Klebsiella aerogenes by neonates.

    abstract::The acquisition of Klebsiella aerogenes by neonates born in hospital has been studied by sero- and klebecin typing. This organism was more commonly carried in the bowel of breast-fed babies than of bottle-fed babies. Only very rarely did babies acquire strains of K. aerogenes from their mothers. K. aerogenes was widel...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-12-2-201

    authors: Shinebaum R,Cooke EM,Brayson JC

    更新日期:1979-05-01 00:00:00

  • Analysis of methods commonly used for glycopeptide and oxazolidinone susceptibility testing in Enterococcus faecium isolates.

    abstract::The susceptibility to teicoplanin, vancomycin and linezolid of 30 clinical isolates of Enterococcus faecium was tested by Vitek 2, Phoenix, Etest, broth microdilution and disc diffusion tests. The vanA and vanB resistance genes and the 23S rRNA gene G2576T mutation were detected by PCR and PCR-RFLP, respectively. Resi...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.016444-0

    authors: Raponi G,Ghezzi MC,Gherardi G,Lorino G,Dicuonzo G

    更新日期:2010-06-01 00:00:00

  • Antifungal susceptibilities, biofilms, phospholipase and proteinase activities in the Candida rugosa complex and Candida pararugosa isolated from tertiary teaching hospitals.

    abstract:PURPOSE:Non-albicansCandida species have emerged as fungal pathogens that cause invasive infections, with many of these species displaying resistance to commonly used antifungal agents. This study was confined to studying the characteristics of clinical isolates of the C. rugosa complex and C. pararugosa species. METH...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000940

    authors: Peremalo T,Madhavan P,Hamzah S,Than L,Wong EH,Nasir MDM,Chong PP,Ng KP

    更新日期:2019-03-01 00:00:00

  • The special affinity of particular types of Proteus mirabilis for the urinary tract.

    abstract::The strains of Proteus species found in significant numbers and as pure cultures in urine from 217 individuals were isolated, identified to species level and typed for proticine production (P type) and proticine sensitivity (S type) to give their P/S type. Urinary-tract infections with Proteus, principally P. mirabili...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-12-1-1

    authors: Senior BW

    更新日期:1979-02-01 00:00:00

  • Influence of normal microbiota on some aspects of the immune response during experimental infection with Trypanosoma cruzi in mice.

    abstract::To study the influence of normal associated microbiota on systemic immunological responses during experimental Chagas' disease, germ-free and conventional NIH Swiss mice were infected with Y strain of Trypanosoma cruzi. Although no statistical differences in mortality and parasitaemia were found, conventional mice sho...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.45657-0

    authors: Duarte R,Silva AM,Vieira LQ,Afonso LCC,Nicoli JR

    更新日期:2004-08-01 00:00:00

  • Molecular analysis of Streptococcus pneumoniae clones causing invasive disease in children in Singapore.

    abstract::Streptococcus pneumoniae remains a leading cause of serious paediatric disease. However, there are few published epidemiological data regarding invasive pneumococcal disease (IPD) in many countries in South East Asia, including Singapore. Baseline data for IPD are essential to inform policy regarding pneumococcal conj...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.030007-0

    authors: Jefferies JMC,Tee WSN,Clarke SC

    更新日期:2011-06-01 00:00:00

  • Successfully treated spondylodiscitis due to a previously unreported mycobacterium.

    abstract::A non-tuberculous mycobacterium was isolated, following a vertebral needle aspiration, from the blood of a patient with severe spondylodiscitis. The strain turned out to be different from any known mycobacterial species and was quite drug-susceptible in vitro. The patient improved markedly following treatment with mer...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.46256-0

    authors: Tortoli E,Mantella A,Mariottini A,Mazzarelli G,Pecile P,Rogasi PG,Sterrantino G,Fantoni E,Leoncini F

    更新日期:2006-01-01 00:00:00

  • Plasmid associated virulence properties of environmental isolates of Aeromonas hydrophila.

    abstract::The plasmid profiles, and their association with antimicrobial resistance, of 60 strains of Aeromonas hydrophila isolated from fish, shellfish and water were investigated. Only two strains were susceptible to all the antimicrobial agents tested; the highest incidences of resistance were to tetracycline (96.7%), prysta...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-35-5-264

    authors: Borrego JJ,Moriñigo MA,Martinez-Manzanares E,Bosca M,Castro D,Barja JL,Toranzo AE

    更新日期:1991-11-01 00:00:00

  • Identification and evaluation of LPS antigen for serodiagnosis of uveitis associated with leptospirosis.

    abstract::Leptospirosis is a widespread zoonotic disease that affects all mammals in different parts of the world. Though there are many commercial kits available for the diagnosis of systemic leptospirosis, the nature of the antigen has not been described. Therefore, identification of a specific antigen is important. Since ocu...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.05120-0

    authors: Priya CG,Bhavani K,Rathinam SR,Muthukkaruppan VR

    更新日期:2003-08-01 00:00:00

  • Manual curation and reannotation of the genomes of Clostridium difficile 630Δerm and C. difficile 630.

    abstract:PURPOSE:We resequenced the genome of Clostridium difficile 630Δerm (DSM 28645), a model strain commonly used for the generation of insertion mutants. METHODOLOGY:The genome sequence was obtained by a combination of single-molecule real-timeand Illumina sequencing technology. RESULTS:Detailed manual curation and compa...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000427

    authors: Dannheim H,Riedel T,Neumann-Schaal M,Bunk B,Schober I,Spröer C,Chibani CM,Gronow S,Liesegang H,Overmann J,Schomburg D

    更新日期:2017-03-01 00:00:00

  • Comparison of conventional culture with SeptiFast real-time PCR for microbial pathogen detection in clinical specimens other than blood.

    abstract::Early detection of aetiological agents is pivotal for adequate therapy for bacterial infections. Although culture is still considered the mainstay for laboratory diagnosis, it often lacks sensitivity, especially in patients already treated with antibiotics. The present study investigated the potential clinical utility...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.034280-0

    authors: Mencacci A,Leli C,Cardaccia A,Montagna P,Moretti A,Bietolini C,Meucci M,Perito S,Cenci E,Bistoni F

    更新日期:2011-12-01 00:00:00

  • A large food-borne outbreak of campylobacteriosis in kindergartens and primary schools in Pescara, Italy, May-June 2018.

    abstract::Introduction. In May-June 2018, an outbreak of campylobacteriosis involved students and school staff from kindergartens and primary schools in Pescara, southern Italy.Aim. We present details of the epidemiological and microbiological investigation, and the findings of the analytical study, as well as the implemented c...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.001262

    authors: Sorgentone S,Busani L,Calistri P,Robuffo G,Bellino S,Acciari V,Ferri M,Graziani C,Antoci S,Lodi F,Alfonsi V,Cammà C,Fazii P,Andrianou X,Cito F,Lombardi G,Centorotola G,D'Amario M,D'Alterio N,Savini V,De Massis F,

    更新日期:2021-01-21 00:00:00

  • Effects of sub-MIC concentrations of antibiotics on growth of and toxin production by Clostridium difficile.

    abstract::Effects on growth and toxin A production of sub-MIC concentrations of six different antibiotics were investigated in three strains of Clostridium difficile: reference strain NCTC 11223, a fully sequenced strain (630) and a locally endemic isolate (strain 338a). The antibiotics chosen for investigation were the agents ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.05387-0

    authors: Drummond LJ,Smith DGE,Poxton IR

    更新日期:2003-12-01 00:00:00

  • Factors determining the susceptibility of mice to experimental phycomycosis.

    abstract::Various factors influencing the susceptibility of C3H mice to lethal infection by the phycomycete Absidia ramosa were examined. Mice 19-21 days old were exposed to graded doses of A. ramosa spores by various routes. After intravenous inoculation with doses in excess of 10(3) viable units, a variable proportion of mice...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-8-4-551

    authors: Corbel MJ,Eades SM

    更新日期:1975-11-01 00:00:00