A paradigm for the molecular identification of Mycobacterium species in a routine diagnostic laboratory.

Abstract:

:The aim of this study was to improve the identification of Mycobacterium species in the context of a UK teaching hospital. Real-time PCR assays were established to enable the rapid differentiation between Mycobacterium tuberculosis (MTB) complex and Mycobacterium species other than tuberculosis (MOTT), followed by 16S rRNA gene sequencing for the speciation of MOTT. Real-time PCR assays gave comparable results to those from the reference laboratory. The implementation of these PCR assays using an improved bead extraction method has enhanced the mycobacterial diagnostic service at the Royal Free Hospital by providing a rapid means of differentiating between MTB complex and MOTT, and would be simple to implement in similar laboratories. Sequence analysis successfully identified a range of Mycobacterium spp. representative of those encountered in the clinical setting of the authors, including Mycobacterium avium complex, Mycobacterium fortuitum group, Mycobacterium chelonae-Mycobacterium abscessus group, Mycobacterium xenopi and Mycobacterium gordonae. It provides a useful tool for the identification of MOTT when clinically indicated.

journal_name

J Med Microbiol

authors

Williams KJ,Ling CL,Jenkins C,Gillespie SH,McHugh TD

doi

10.1099/jmm.0.46855-0

subject

Has Abstract

pub_date

2007-05-01 00:00:00

pages

598-602

issue

Pt 5

eissn

0022-2615

issn

1473-5644

journal_volume

56

pub_type

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