Abstract:
:A total of 3429 isolations of verocytotoxin-producing Escherichia coli O157 (VTEC O157) was confirmed from human sources in England and Wales during the period 1995-1998. The largest annual total was 1087 in 1997. Most infections occurred in the third quarter of each year. The overall rate of infection ranged from 1.28 to 2.10/100,000 population and showed regional variation. The highest incidence was in children aged 1-4 years. Annually, between 5% and 11% of strains were from patients who had travelled abroad. There were 67 general outbreaks of infection represented by 407 (11.9%) VTEC O157 isolates. Outbreaks involved transmission by contaminated food or water, person-to-person spread and direct or indirect animal contact, and five were associated with foreign travel. The majority (76%) of strains carried verocytotoxin (VT) 2 genes and 23.3% were VT1+VT2. Most strains had the flagellar antigen H7, but c. 14% were non-motile. Approximately 20% of isolates were resistant to antimicrobial agents, predominantly streptomycin, sulphonamides and tetracycline. In addition to VTEC O157, strains of serogroup O157 that did not possess VT genes were identified. These were either derivatives of VTEC O157 that had lost VT genes or were strains with H antigens other than H7 that have never been associated with VT production. Strains of VTEC other than O157 were characterised. Most were associated with diarrhoea, bloody diarrhoea or haemolytic uraemic syndrome and had virulence markers in addition to VT.
journal_name
J Med Microbioljournal_title
Journal of medical microbiologyauthors
Willshaw GA,Cheasty T,Smith HR,O'Brien SJ,Adak GKdoi
10.1099/0022-1317-50-2-135keywords:
subject
Has Abstractpub_date
2001-02-01 00:00:00pages
135-142issue
2eissn
0022-2615issn
1473-5644journal_volume
50pub_type
杂志文章abstract::Helicobacter pylori is classified as a class I carcinogenic factor and its persistent colonization in the stomach induces gastric cancer. Cancerous Inhibitor of PP2A (CIP2A) is a newly identified oncoprotein overexpressed in gastric cancer. Serving as a key oncoprotein, CIP2A also participates in regulation of senesce...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.014704-0
更新日期:2010-03-01 00:00:00
abstract::Clostridium difficile-associated diarrhoea (CDAD) is the most common hospital-acquired diarrhoea, and is a major type of gastroenteritis infection in nursing homes and facilities for the elderly. In this study the antimicrobial activity of the two-component lantibiotic, lacticin 3,147, against a range of genetically d...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.47085-0
更新日期:2007-07-01 00:00:00
abstract::Campylobacter jejuni, when left on blood agar for prolonged periods, was found to survive better in air than under micro-aerobic conditions. After a period of 2-3 days in air, all strains of C. jejuni examined grew freely in air on subculture, and could be further subcultured apparently indefinitely in air. This adapt...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-38-2-145
更新日期:1993-02-01 00:00:00
abstract::In a prospective study between February 2003 and June 2004, stool specimens of children less than 2 years of age with diarrhoea (n=218) and without diarrhoea (n=86), living in Vitória, Espírito Santo, Brazil, were examined for the presence of diarrhoeagenic Escherichia coli. E. coli isolates were tested by colony blot...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.47660-0
更新日期:2008-03-01 00:00:00
abstract:PURPOSE:Non-host-adapted Salmonella serovars, including the common human food-borne pathogen Salmonella enterica serovar Typhimurium (S. Typhimurium), are opportunistic pathogens that can colonize food-producing animals without causing overt disease. Interventions against Salmonella are needed to enhance food safety, p...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000482
更新日期:2017-05-01 00:00:00
abstract::Brevundimonas diminuta is rarely associated with invasive infections. We report the case of a previously healthy young man with pleural effusion, in which B. diminuta was recovered but incorrectly identified as Kingella kingae when it was freshly isolated. Consequently, the misidentification resulted in initial treatm...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.045013-0
更新日期:2013-03-01 00:00:00
abstract::The aim of this study was to conduct a comparative analysis of the 16S rRNA gene fragment nucleotide sequences for Anaplasma phagocytophilum strains detected in the blood of horses from various parts of Europe. The study comprised 234 horses that had had contact with ticks. Using PCR, the genetic material of A. phagoc...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.058636-0
更新日期:2013-12-01 00:00:00
abstract::Purpose. Acute bacterial meningitis continues to be a potentially life threatening condition. Hospital-acquired meningitis is rapidly increasing and adding an immense burden to the health system due to the emergence of multidrug resistance isolates. The purpose of this study is to find the antibiotic susceptibility pa...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.001072
更新日期:2019-11-01 00:00:00
abstract::Clostridium difficile is the most common cause of antibiotic-associated diarrhoea. Antibiotics are presumed to disturb the normal intestinal microbiota, leading to depletion of the barrier effect and colonization by pathogenic bacteria. This first step of infection includes adherence to epithelial cells. We investigat...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.47676-0
更新日期:2008-06-01 00:00:00
abstract::Accurate susceptibility results on antibiotic-resistant bacteria are essential for proper treatment of infections. In this study, 100 metallo-β-lactamase (MBL)-producing strains and 95 isolates with Klebsiella pneumoniae carbapenemase (KPC) were tested for carbapenem susceptibility using two automated platforms, the P...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000067
更新日期:2015-06-01 00:00:00
abstract::Erysipelothrix rhusiopathiae is a zoonotic pathogen that causes erysipelas in a variety of animals. In humans, in contrast to the cutaneous form called erysipeloid, which is an occupational disease and common in individuals who handle raw meat and fish, invasive systemic infections are unusual. E. rhusiopathiae expres...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.001252
更新日期:2020-10-01 00:00:00
abstract::The aim of this study was the analysis of the cytokine response in BALB/c mice infected with the highly virulent RH or the weakly virulent Beverley strains of Toxoplasma gondii. Analysis of cytokine messages showed increased expression of IL12, IFN-gamma and TNF-alpha, but not IL4 mRNAs in spleen cells after infection...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.04860-0
更新日期:2003-10-01 00:00:00
abstract:PURPOSE:Despite hundreds of reports on the isolation of Nocardia from clinical samples, the presence and diversity of Nocardia species that are capable of survival in a harsh and adverse condition, such as a hospital environment, have not been comprehensively studied. The aim of this study was to assess Nocardia specie...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000436
更新日期:2017-03-01 00:00:00
abstract::Toxoplasma gondii infection in humans is routinely assessed by serological means. Here, the authors attempted to compare the response of different Toxoplasma strains to serological tests and to evaluate the antigenic profiles of the RH and RH Ankara (TRH) strains with Western blotting. Anti-Toxoplasma IgG antibodies o...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.46712-0
更新日期:2006-10-01 00:00:00
abstract::Mice immunised by the oral or intraperitoneal route with a live aromatic-dependent strain of Salmonella typhimurium exhibited significantly less protection against oral challenge with 50 LD50 of an ovine isolate of S. typhimurium (12313) than when a bovine isolate with the same O antigens and phage-type as strain 1231...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-24-1-11
更新日期:1987-08-01 00:00:00
abstract::The fungal pathogen Cryptococcus neoformans has a predilection for the central nervous system (CNS), resulting in devastating meningoencephalitis. At present, it is unclear how C. neoformans traverses the blood-brain barrier (BBB) and causes CNS infection. The present study has examined and characterized the interacti...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.05230-0
更新日期:2003-11-01 00:00:00
abstract::In-vitro proteinase production by oral Candida albicans isolates from patients with and without HIV infection (18 isolates from each group) was assessed by image analysis of a plate assay, with bovine serum albumin (BSA) as a substrate. The effect of sub-minimal inhibitory concentrations (sub-MICs) of nystatin, amphot...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-44-4-311
更新日期:1996-04-01 00:00:00
abstract::Enteropathogenic Escherichia coli (EPEC) is an important aetiological agent of persistent infantile diarrhoea. EPEC pathogenicity is not mediated through known toxins and the role played by outer-membrane proteins (OMPs) in the initial adherence of the bacterium, intimate attachment to epithelial cells and ultimately ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/0022-1317-50-7-602
更新日期:2001-07-01 00:00:00
abstract::Chickens were immunised with a preparation of purified 14-kDa fimbriae of Salmonella serotype Enteritidis (SEF 14) to raise egg-yolk antibodies for protection trials in mice against subsequent challenge-exposure with the homologous strain of Enteritidis. A pronounced specificity of egg-yolk antibodies against the 14-k...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-41-1-29
更新日期:1994-07-01 00:00:00
abstract::Rickettsia rickettsii has limited adverse effects on its arthropod vector, but causes severe disease in man. To model differences in host-parasite interaction, R. rickettsii growth and protein expression were examined at temperatures reflective of host environment in the tick cell lines DALBE3 and IDE2, the human endo...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-46-10-839
更新日期:1997-10-01 00:00:00
abstract::Invasive aspergillosis is a significant cause of morbidity and mortality within at-risk groups. Directed antifungal chemotherapy, guided by effective screening algorithms that incorporate reliable and validated molecular assays, reduces the morbidity associated with empirical administration and allows earlier diagnosi...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.076315-0
更新日期:2014-09-01 00:00:00
abstract::Bovine lactoferricin, a pepsin-generated antimicrobial peptide from bovine lactoferrin active against a wide range of bacteria, was tested for its ability to influence the adhesion and invasion of Yersinia enterocolitica and Yersinia pseudotuberculosis in HEp-2 cells. The addition of non-cytotoxic and non-bactericidal...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.05410-0
更新日期:2004-05-01 00:00:00
abstract::Since 2007, there has been a re-emergence of cholera outbreaks in northern Vietnam. To understand the molecular epidemiological relatedness and determine the antibiotic susceptibility profiles of responsible V. cholerae O1 outbreak strains, a representative collection of 100 V. cholerae O1 strains was characterized. V...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.034744-0
更新日期:2012-03-01 00:00:00
abstract::The prevalence of chlamydial DNA determined by PCR and in-situ hybridisation (ISH) in fresh tissue specimens (endometrium, fallopian tube and ovary) was investigated in 33 women presenting with ectopic pregnancy (EP), 14 women with tubal factor infertility (TFI) and 50 control patients from the UK and the West Indies....
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/0022-1317-50-10-902
更新日期:2001-10-01 00:00:00
abstract::The acquisition of Klebsiella aerogenes by neonates born in hospital has been studied by sero- and klebecin typing. This organism was more commonly carried in the bowel of breast-fed babies than of bottle-fed babies. Only very rarely did babies acquire strains of K. aerogenes from their mothers. K. aerogenes was widel...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-12-2-201
更新日期:1979-05-01 00:00:00
abstract::Leucocyte-egested material was harvested after the quantitative in-vitro phagocytosis of Neisseria meningitidis by rabbit or mouse polymorphonuclear leucocytes. The egested material was injected subcutaneously into rabbits and followed 24 h later with an intravenous injection of what would by itself have been a non-le...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-9-4-451
更新日期:1976-11-01 00:00:00
abstract::Recent studies have shown that the intestinal microbiota is essential for the pathogenicity but not for the multiplication of Giardia duodenalis in the intestinal lumen. The microbial components responsible for this phenomenon are not known. Twenty-eight facultative and three strictly anaerobic micro-organisms were is...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/0022-1317-49-3-209
更新日期:2000-03-01 00:00:00
abstract::The in vitro activity of voriconazole was compared with those of fluconazole and itraconazole against 270 clinical isolates of yeasts from the mouths of patients receiving palliative care for advanced cancer. A broth micro-dilution assay as described by the National Committee for Clinical Laboratory Standards was empl...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.45720-0
更新日期:2005-10-01 00:00:00
abstract::The agglutination of 218 clinical isolates and three ATCC type strains of "Streptococcus milleri" was tested with 25 different lectins from plants and fungi. An agglutination reaction with one or more lectins was observed with 42 isolates when the cells were untreated. After trypsinisation of the bacteria, 109 strains...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-41-1-14
更新日期:1994-07-01 00:00:00
abstract::Gardnerella vaginalis is an important component of the human vaginal microflora. It is proposed to play a key role in the pathogenesis of bacterial vaginosis (BV), the most common vaginal condition. Here we describe the development, validation and comparative analysis of a novel molecular approach capable of G. vagina...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.066407-0
更新日期:2014-02-01 00:00:00