Laboratory challenges in the diagnosis of hepatitis E virus.

abstract：:Burkholderia pseudomallei is the causative agent of human and animal melioidosis. The role of quorum sensing (QS) in the in vivo pathogenicity of B. pseudomallei via inhalational exposure of BALB/c mice and intraperitoneal challenge of Syrian hamsters has not been reported. This investigation demonstrates that B. pseu...

journal_title：Journal of medical microbiology

authors： Ulrich RL,DeShazer D,Brueggemann EE,Hines HB,Oyston PC,Jeddeloh JA

更新日期：2004-11-01 00:00:00

abstract：:To clarify further the role of chemotaxis in Helicobacter pylori colonization, the in vitro bacterium response to human plasma and bile (secretions containing chemoeffector compounds that are present in the gastric mucus layer) was examined. Human plasma, after dilution to 1 % (v/v) with buffer, was found to be a chem...

journal_title：Journal of medical microbiology

authors： Worku ML,Karim QN,Spencer J,Sidebotham RL

更新日期：2004-08-01 00:00:00

abstract：:Acinetobacter baumannii is an emerging multidrug-resistant pathogen and very little information is available regarding its imipenem resistance in Latin American countries such as Bolivia. This study investigated the antimicrobial resistance profile of 46 clinical strains from different hospitals in Cochabamba, Bolivia...

journal_title：Journal of medical microbiology

authors： Sevillano E,Fernández E,Bustamante Z,Zabalaga S,Rosales I,Umaran A,Gallego L

更新日期：2012-01-01 00:00:00

abstract：:In recent years, several plasmids harbouring genes encoding phosphoethanolamine transferases conferring colistin resistance have been described in multiple Enterobacteriaceae species. Avian Pathogenic E. coli (APEC) causes colibacillosis and is responsible for a considerable proportion of the disease burden in commerc...

journal_title：Journal of medical microbiology

authors： Hornsey M,Betts JW,Mehat JW,Wareham DW,van Vliet AHM,Woodward MJ,La Ragione RM

更新日期：2019-01-01 00:00:00

abstract：:Subtractive hybridisation was used to screen for and identify conserved DNA sequences associated with clinical, clonal populations of Staphylococcus aureus. DNA from S. aureus strain ISP8 (a clinical isolate) was digested with TaqI and hybridised with randomly fragmented pooled DNA from 10 non-clinical (community) iso...

journal_title：Journal of medical microbiology

authors： el-Adhami WA,Stewart PR,Matthaei KI

更新日期：1997-12-01 00:00:00

abstract：:The prevalence of community-acquired meticillin-resistant Staphylococcus aureus (CA-MRSA) strains has become a serious problem worldwide. The aim of this study was to investigate the annual transitions of MRSA strains with the CA-MRSA feature, which were identified as SCCmec type IV or V, in a hospital setting in Japa...

journal_title：Journal of medical microbiology

authors： Ito A,Nakaminami H,Fujii T,Utsumi K,Noguchi N

更新日期：2015-07-01 00:00:00

abstract：:Campylobacter fetus subsp. fetus infection can occur in female sheep, causing infertility or abortion. Despite extensive research on the effect of these bacteria on female fertility, little research has been done on the influence of C. fetus subsp. fetus on the male factor. Our objective was to examine the influence o...

journal_title：Journal of medical microbiology

authors： Zan Bar T,Yehuda R,Hacham T,Krupnik S,Bartoov B

更新日期：2008-11-01 00:00:00

abstract：:During a 3-year period, 771 rectal swabs were taken from abacteriuric school-children. Out of 709 E. coli strains, each isolated from one faecal specimen, 102 were found to be resistant to one or more antibacterial agents, and 607 to be fully sensitive. Another 204 resistant strains were found by selection for antibio...

journal_title：Journal of medical microbiology

authors： Lidin-Janson G,Falsen E,Jodal U,Kaijser B,Lincoln K

更新日期：1977-08-01 00:00:00

abstract：:A series of 178 strains of Escherichia coli, highly resistant to trimethoprim, isolated from hospital patients and patients in the community between 1979 and 1983, was examined for the presence of Tn7 on a plasmid or on the chromosome only, by transposition to RP4 and restriction endonuclease digestion with Hind III. ...

journal_title：Journal of medical microbiology

authors： Kraft CA,Timbury MC,Platt DJ

更新日期：1986-09-01 00:00:00

abstract：:Intratracheal administration of purified Pseudomonas aeruginosa exoenzyme S elicited extensive, grossly observable damage in the rat lung within 2 h. Light and electronmicroscopy revealed injury and necrosis of bronchial epithelium, type I pneumocytes and capillary endothelial cells after 1 h; associated haemorrhage, ...

journal_title：Journal of medical microbiology

authors： Woods DE,Hwang WS,Shahrabadi MS,Que JU

更新日期：1988-06-01 00:00:00

abstract：:Fifty-seven strains of acinetobacter-like organisms were isolated over a period of 26 months from the ears of 55 patients with acute or chronic otitis media, or otitis externa, and one strain was isolated in a survey of 50 normal ears. After comparison with eight reference strains, 32 of the isolates were identified a...

journal_title：Journal of medical microbiology

authors： Dadswell JV

更新日期：1976-08-01 00:00:00

abstract：:Bacterial activity and growth were monitored by following the changes of electrical impedance of cultures in liquid media. The signal is expressed automatically as a curve similar to grwoth curves produced by other methods. The technique offers a new, rapid and sensitive means of detecting active micro-organisms and i...

journal_title：Journal of medical microbiology

authors： Ur A,Brown DF

更新日期：1975-02-01 00:00:00

abstract：:A total of 1878 non-duplicate clinical Escherichia coli isolates (comprising 1711 urinary isolates and 167 blood-culture isolates), which were collected from multiple centres in Hong Kong during 1996-2008, were used to investigate the prevalence and molecular epidemiology of plasmid-mediated fosfomycin (fos) resistanc...

journal_title：Journal of medical microbiology

authors： Ho PL,Chan J,Lo WU,Lai EL,Cheung YY,Lau TCK,Chow KH

更新日期：2013-11-01 00:00:00

abstract：:Motile curved rods seen in vaginal secretions have been isolated on Columbia agar supplemented with 5% human blood and vitamin K. Growth occurred anaerobically and in 5% oxygen but not in more aerobic conditions. There were two distinct groups of these organisms, distinguishable by morphology, biochemical activity and...

journal_title：Journal of medical microbiology

authors： Sprott MS,Ingham HR,Pattman RS,Eisenstadt RL,Short GR,Narang HK,Sisson PR,Selkon JB

更新日期：1983-05-01 00:00:00

abstract：:Fusobacterium necrophorum, a Gram-negative anaerobe, causes a variety of necrotic infections in humans and animals. There are two subspecies: subsp. necrophorum and subsp. funduliforme. In cattle, subsp. necrophorum is more prevalent and production of leukotoxin is a major virulence factor. The leukotoxin operon (lktB...

journal_title：Journal of medical microbiology

authors： Tadepalli S,Stewart GC,Nagaraja TG,Narayanan SK

更新日期：2008-02-01 00:00:00

abstract：:The toxic effects of the haemorrhagic toxin of Clostridium sporogenes were studied in mice, rats, guinea-pigs and rabbits, and in various cultured cells. In rabbits, but not in the other animals, intradermal injection with crude toxin and its injection into a ligated intestinal loop caused haemorrhage in both the skin...

journal_title：Journal of medical microbiology

authors： Hara-Kudo Y,Ogura A,Noguchi Y,Kumagai S

更新日期：1997-04-01 00:00:00

abstract：:Breakthrough contamination of tuberculosis (TB) cultures is a problem in that it allows the overgrowth of another bacterium present in the sputum specimen, which can potentially mask the presence of Mycobacterium tuberculosis. The aim of this study was to isolate and characterize the bacterial organisms responsible fo...

journal_title：Journal of medical microbiology

authors： McClean M,Stanley T,Stanley S,Maeda Y,Goldsmith CE,Shepherd R,Millar BC,Dooley JS,Moore JE

更新日期：2011-09-01 00:00:00

abstract：:Introduction. Pseudomonas aeruginosa is an important Gram-negative pathogen that is intrinsically multidrug-resistant (MDR) and frequently associated with healthcare-associated outbreaks. With increasing resistance to antibiotics and with very few novel drugs under development, clinicians often use combinations to tre...

journal_title：Journal of medical microbiology

authors： Betts JW,Hornsey M,Higgins PG,Lucassen K,Wille J,Salguero FJ,Seifert H,La Ragione RM

更新日期：2019-10-01 00:00:00

abstract：:Bacteriophages in different stages of maturation were found in thin sections of a clinical isolate of Helicobacter (Campylobacter) pylori. Mature phage heads measured 70 x 60 nm and the tail at least 120 nm. Lysogeny was maintained during subculture on blood agar for more than 3 months after isolation from a gastric b...

journal_title：Journal of medical microbiology

authors： Schmid EN,von Recklinghausen G,Ansorg R

更新日期：1990-06-01 00:00:00

abstract：:The opsonic and protective capacities of rabbit antisera against Escherichia coli O, K and core-glycolipid cell-wall antigens were compared with specific antibody titres as measured by agglutination and enzyme-linked immunosorbent assay. Anti-O antisera were opsonic and protective against two noncapsulate strains. Onl...

journal_title：Journal of medical microbiology

authors： van Dijk WC,Verbrugh HA,van Erne-van der Tol ME,Peters R,Verhoef J

更新日期：1981-11-01 00:00:00

abstract：:The morbidity and mortality rates in patients with cystic fibrosis (CF) are significantly affected by infections with Burkholderia cepacia complex. In a Czech CF Centre, the prevalence of the infection reached up to 30 %, with the majority of patients found to be infected with Burkholderia cenocepacia (formerly genomo...

journal_title：Journal of medical microbiology

authors： Drevinek P,Vosahlikova S,Cinek O,Vavrova V,Bartosova J,Pohunek P,Mahenthiralingam E

更新日期：2005-07-01 00:00:00

abstract：:As well as conventional methods such as immunodiffusion, ELISA, or agglutination for the detection of toxin production in Staphylococcus aureus, amplification techniques like PCR allow a very sensitive and specific identification of the genes responsible for enterotoxin B and C, and TSST-1 production. These toxins mig...

journal_title：Journal of medical microbiology

authors： Schmitz FJ,Steiert M,Hofmann B,Verhoef J,Hadding U,Heinz HP,Köhrer K

更新日期：1998-04-01 00:00:00

abstract：:Mice were vaccinated with lipopolysaccharide (LPS) from Leptospira interrogans serovar pomona or hardjo, or with the polysaccharide (PS) fraction of the LPS, or with an immunoconjugate of PS and diphtheria toxoid (DT). Maximum agglutinin titres were found 6-10 weeks after vaccination with LPS or PS-DT conjugate; the l...

journal_title：Journal of medical microbiology

authors： Midwinter A,Faine S,Adler B

更新日期：1990-11-01 00:00:00

abstract：:Conventional culture and drug susceptibility testing (DST) methods for Mycobacterium tuberculosis are laborious and time consuming. For this reason alternative rapid culture and DST techniques are urgently needed to shorten the time for drug-resistance detection. A total of 222 smear-positive sputum samples were evalu...

journal_title：Journal of medical microbiology

authors： Imperiale BR,Morcillo NS,Palomino JC,Vandamme P,Martin A

更新日期：2014-04-01 00:00:00

abstract：:The heparin-binding properties of six different species of coagulase-negative staphylococci were examined by a particle agglutination assay. Heparin (mol. wt 4000-6000), mildly treated with sodium periodate, was covalently coupled to amino-modified latex beads (0.72 micron diameter). The particle agglutination assay w...

journal_title：Journal of medical microbiology

authors： Pascu C,Hirmo S,Ljungh A,Wadström T

更新日期：1996-10-01 00:00:00

abstract：:The aim of this work was to determine whether diazepam could induce the multiple antibiotic resistance (Mar) phenotype in Klebsiella pneumoniae and Escherichia coli strains. The Mar phenotype is characterized by decreased susceptibility to multiple antibiotics due to the loss of porins and/or increased expression of a...

journal_title：Journal of medical microbiology

authors： Tavío MM,Vila J,Perilli M,Casañas LT,Maciá L,Amicosante G,Jiménez de Anta MT

更新日期：2004-11-01 00:00:00

abstract：:A novel aminopeptidase was purified by high performance liquid chromatography from a cytosoluble 100,000 g extract of Candida albicans on the basis of its ability to cleave L-arginine 7-amino-4-methylcoumarin. The purification factor was 36 and the yield was 20%. The native enzyme had a mol. wt of 52 kDa as demonstrat...

journal_title：Journal of medical microbiology

authors： el Moudni B,Rodier MH,Barrault C,Ghazali M,Jacquemin JL

更新日期：1995-10-01 00:00:00

abstract：:Monoclonal antibodies (MAbs) N-59 and RU-1 were produced by immunisation of mice with streptokinase secreted by Streptococcus group A, type 12, strain A374 isolated from a patient with post-streptococcal glomerulonephritis (PSGN) and were characterised by Western blot analysis. MAb N-59 recognised antigenic determinan...

journal_title：Journal of medical microbiology

authors： Ohkuni H,Todome Y,Yoshimura K,Yamamoto T,Suzuki H,Yokomuro K,Johnston KH,Zabriskie JB

更新日期：1991-07-01 00:00:00

abstract：:Growth of Candida albicans biofilms and production of extracellular matrix were monitored by dry weight, colorimetric and radioisotope assays, and by scanning electron microscopy. Under static incubation conditions synthesis of matrix material was minimal, but increased dramatically when developing biofilms were subje...

journal_title：Journal of medical microbiology

authors： Hawser SP,Baillie GS,Douglas LJ

更新日期：1998-03-01 00:00:00

abstract：:The microbiota of the human oropharynx plays an important role in health through involvement in the aetiology of infection and the carriage of adventitious pathogens. Despite this, there are few models available for the preclinical assessment of novel antimicrobials directed to the human throat. We have profiled bacte...

journal_title：Journal of medical microbiology

authors： Humphreys GJ,McBain AJ

更新日期：2013-06-01 00:00:00