Abstract:
:In recent years, several plasmids harbouring genes encoding phosphoethanolamine transferases conferring colistin resistance have been described in multiple Enterobacteriaceae species. Avian Pathogenic E. coli (APEC) causes colibacillosis and is responsible for a considerable proportion of the disease burden in commercial poultry flocks, and may be linked to zoonotic infections in humans. Here, we describe the genotypic and phenotypic characteristics of a multidrug-resistant APEC ST69 isolate (APECA2), recovered in 2016 from a diseased broiler at post-mortem examination in Germany. The isolate was resistant to several antibiotics of human and veterinary importance, including colistin. The mcr-1 gene was detected on a mobile genetic element located on an IncHI2/ST4 plasmid, which was characterized using long-read Nanopore and short-read Illumina sequencing of purified plasmid. Isolate APECA2 displayed resistance to chicken serum and harbours numerous virulence genes. This study highlights the public health importance of enhanced antimicrobial resistance surveillance and strict antimicrobial stewardship in human and veterinary healthcare.
journal_name
J Med Microbioljournal_title
Journal of medical microbiologyauthors
Hornsey M,Betts JW,Mehat JW,Wareham DW,van Vliet AHM,Woodward MJ,La Ragione RMdoi
10.1099/jmm.0.000882subject
Has Abstractpub_date
2019-01-01 00:00:00pages
111-114issue
1eissn
0022-2615issn
1473-5644journal_volume
68pub_type
杂志文章abstract:PURPOSE:Despite hundreds of reports on the isolation of Nocardia from clinical samples, the presence and diversity of Nocardia species that are capable of survival in a harsh and adverse condition, such as a hospital environment, have not been comprehensively studied. The aim of this study was to assess Nocardia specie...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000436
更新日期:2017-03-01 00:00:00
abstract::The relationship between vaginal carriage and subsequent neonatal acquisition of Clostridium difficile was investigated. Vaginal carriage of C. difficile was detected in 11% of women attending the Department of Genital Medicine Clinic. C. difficile was isolated from the vagina in 18% of 50 mothers before delivery, and...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-18-1-47
更新日期:1984-08-01 00:00:00
abstract:PURPOSE:This study investigated the efficacy of the essential mineral, selenium (sodium selenite), in reducing the toxin production, spore outgrowth and antibiotic resistance of Clostridium difficile in vitro. METHODOLOGY:Two hypervirulent C. difficile isolates were cultured in brain heart infusion broth with and with...
journal_title:Journal of medical microbiology
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doi:10.1099/jmm.0.001008
更新日期:2019-07-01 00:00:00
abstract::The opsonic requirements of Helicobacter pylori were investigated in a series of experiments with human polymorphonuclear leucocytes (PMNL). Pre-incubation of H. pylori with pooled normal human serum (NHS) in concentrations of 5-20% significantly increased the uptake of radiolabelled bacteria by PMNL. Treatment of the...
journal_title:Journal of medical microbiology
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doi:10.1099/00222615-38-3-209
更新日期:1993-03-01 00:00:00
abstract::To analyse the characteristics of infections caused by Streptococcus dysgalactiae subsp. equisimilis, clinical isolates (n=145) were collected at 11 medical institutions between September 2003 and October 2005. These isolates belonged to Lancefield group A (n=5), group C (n=18) or group G (n=122). Among all isolates, ...
journal_title:Journal of medical microbiology
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doi:10.1099/jmm.0.013201-0
更新日期:2010-01-01 00:00:00
abstract::The distribution of hepatitis C virus (HCV) genotypes among Egyptian patients positive for anti-HCV was determined and their influence, when combined with neu-oncoprotein overexpression, on the development of hepatocellular carcinoma (HCC) was examined. The study groups included asymptomatic carriers (ASC) and patient...
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abstract::Mycobacterium bovis is a pathogen of cattle. The unpasteurized milk of affected cattle is a source of infection in humans. Despite the screening of cattle and the pasteurization of milk, M bovis has not been eradicated. A high index of clinical suspicion is needed in symptomatic patients with a history of possible exp...
journal_title:Journal of medical microbiology
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doi:10.1099/jmm.0.020511-0
更新日期:2010-11-01 00:00:00
abstract::In this study, 250 vaginal samples from patients with vulvovaginal candidosis were inoculated onto two chromogenic media, Albicans ID(2) and Biggy agar, as well as onto Sabouraud chloramphenicol agar, yielding a total of 63 yeast (25.2 %) on all three media. These strains were identified as Candida glabrata in 20 (31....
journal_title:Journal of medical microbiology
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更新日期:2007-06-01 00:00:00
abstract::Providencia alcalifaciens is an invasive enteric pathogen. The present study determined the prevalence of invasive ability in P. alcalifaciens strains isolated in São Paulo, Brazil, mainly from patients with diarrhoea. Invasion of HeLa cells was found in 17 (42%) of 41 strains studied. Most (88%) of the invasive strai...
journal_title:Journal of medical microbiology
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journal_title:Journal of medical microbiology
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doi:10.1099/jmm.0.46123-0
更新日期:2005-09-01 00:00:00
abstract::The major risk factor for Clostridium difficile infection (CDI) is the use of antibiotics owing to the disruption of the equilibrium of the host gut microbiota. To preserve the beneficial resident probiotic bacteria during infection treatment, the use of molecules with selective antibacterial activity enhances the eff...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.080796-0
更新日期:2014-12-01 00:00:00
abstract::Encapsulation of purified tuberculoproteins in liposomes augmented their ability to elicit delayed hypersensitivity reactions in BCG-immune rats. The effect was most marked with a low-molecular-weight tuberculopeptide that was relatively poor at eliciting reactions when in free form. These findings indicate that, in a...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-18-3-429
更新日期:1984-12-01 00:00:00
abstract::In the present study, polyphasic analysis [cultivation, combined with the fingerprinting of individual isolates, and denaturing gradient gel electrophoresis (DGGE)] was applied to study whether similar features concerning the diversity and temporal stability of selected bacterial groups could be detected intra-individ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.47352-0
更新日期:2008-12-01 00:00:00
abstract::A longitudinal analysis was carried out of the colonization by four potential respiratory pathogens - Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis and Staphylococcus aureus - in 165 healthy children (aged 3-7 years) attending three kindergartens and 417 healthy children (aged 7-10 years) att...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.040584-0
更新日期:2012-05-01 00:00:00
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journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-11-4-541
更新日期:1978-11-01 00:00:00
abstract::The objectives of the present study were to evaluate in vitro the production of antagonistic compounds against Gardnerella vaginalis by Lactobacillus strains isolated from women with or without bacterial vaginosis (BV), and to select one of the better Lactobacillus producers of such a substance to be tested in vivo us...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.041962-0
更新日期:2012-08-01 00:00:00
abstract::Pyrolysis mass spectrometry (PyMS), a highly discriminatory method for comparison of isolates, was used to assess the homogeneity of colonies taken from apparently pure cultures of Escherichia coli in 10 urine specimens. Ten randomly selected colonies were subcultured from each urine for comparison. For six urines, th...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-45-5-349
更新日期:1996-11-01 00:00:00
abstract::Staphylococcus aureus produces numerous bi-component toxins, e.g., Panton-Valentine leukocidin (Luk-PVL) and gamma-haemolysin, which consist of type S and F proteins. Previous studies showed that Luk-PVL induces inflammatory mediator release from human granulocytes that might reflect the in-vivo effects, e.g., dermone...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-46-6-479
更新日期:1997-06-01 00:00:00
abstract::Fusobacterium necrophorum, a Gram-negative anaerobe, causes a variety of necrotic infections in humans and animals. There are two subspecies: subsp. necrophorum and subsp. funduliforme. In cattle, subsp. necrophorum is more prevalent and production of leukotoxin is a major virulence factor. The leukotoxin operon (lktB...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.47598-0
更新日期:2008-02-01 00:00:00
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journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000150
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abstract::Early reports of Candida krusei in man describe the organism as a transient, infrequent isolate of minor clinical significance inhabiting the mucosal surfaces. More recently it has emerged as a notable pathogen with a spectrum of clinical manifestations such as fungaemia, endophthalmitis, arthritis and endocarditis, m...
journal_title:Journal of medical microbiology
pub_type: 杂志文章,评审
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更新日期:1994-11-01 00:00:00
abstract::Motile curved rods seen in vaginal secretions have been isolated on Columbia agar supplemented with 5% human blood and vitamin K. Growth occurred anaerobically and in 5% oxygen but not in more aerobic conditions. There were two distinct groups of these organisms, distinguishable by morphology, biochemical activity and...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-16-2-175
更新日期:1983-05-01 00:00:00
abstract::The ability of rabbits to clear an intraperitoneal injection of Pseudomonas aeruginosa in the presence or absence of a surgically implanted peritoneal device was investigated. Sham-operated rabbits without an implant eliminated a P. aeruginosa challenge of 5 x 10(6) cfu/ml; lavage fluid and peritoneal tissues became c...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-36-6-406
更新日期:1992-06-01 00:00:00
abstract::The purpose of this study was to evaluate the anti-chlamydial activities in vitro of liposome-encapsulated doxycycline (Dox) and tetracycline (Tet) in comparison with free Dox and Tet. Dox and Tet encapsulated in cationic (CAL), anionic (ANL) and neutral (NTL) liposomes by sonication, were quantified by high-performan...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-48-7-689
更新日期:1999-07-01 00:00:00
abstract::A multiplex PCR was developed to detect 15 serotypes of Streptococcus suis. This multiplex PCR was separated into two reaction sets. The first set identified nine serotypes (serotypes 1/2, 1, 2, 3, 4, 7, 9, 14 and 16) and the second set identified six serotypes (serotypes 5, 8, 10, 19, 23 and 25). This assay correctly...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.048587-0
更新日期:2012-12-01 00:00:00
abstract::Enteroaggregative Escherichia coli (EAEC), a highly heterogeneous pathotype of E. coli classified as typical and atypical, are an emerging cause of acute and persistent diarrhea. We aimed to investigate whether population living in rural geographic areas, impacts in the heterogeneity, dissemination and antimicrobial s...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.001120
更新日期:2020-01-01 00:00:00
abstract::Carnobacterium species have been isolated from the environment and are not regarded as human pathogens, although they are known to cause disease in fish. Only two reports describing isolation of Carnobacterium species from human pus were found in the literature. We report what we believe to be the first isolation of C...
journal_title:Journal of medical microbiology
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更新日期:2010-04-01 00:00:00
abstract::In Mexico, there is a lack of up-to-date published data that show viruses to be the main cause of acute respiratory infection (ARI). The objective of this study was to estimate the comparative viral frequency between children under the age of 5 years with and without ARI (n = 179 in each group) in a suburban community...
journal_title:Journal of medical microbiology
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doi:10.1099/jmm.0.05007-0
更新日期:2003-07-01 00:00:00
abstract::The aim of this study was to explore the epidemiological and molecular characteristics of Streptococcus pyogenes in children from different cities in mainland China who were diagnosed with scarlet fever, impetigo and pharyngitis, as well as to detect asymptomatic carriers, between 2005 and 2008, and to compare the res...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.042309-0
更新日期:2012-07-01 00:00:00
abstract::Three strains of Plesiomonas shigelloides isolated from patients with diarrhoea were agglutinated with Shigella flexneri 6 antiserum in slide and tube tests. All the strains were also agglutinated with a monoclonal antibody to the common group 1 antigen shared between S. flexneri serotypes and S. dysenteriae type 1. F...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
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更新日期:1993-09-01 00:00:00