Effect of antimycotic agents on the activity of aspartyl proteinases secreted by Candida albicans.

Abstract:

:The inhibitory effect of human immunodeficiency virus (HIV) proteinase inhibitors amprenavir and saquinavir and antifungal agents terbinafine, ketoconazole, amphotericin B and ciclopiroxolamine on aspartyl proteinases (Saps) secreted by Candida albicans was tested in an in vitro spectophotometric assay. As expected, both HIV proteinase inhibitors showed a significant inhibitory effect on Sap activity, which was comparable to that of the classical aspartyl proteinase inhibitor pepstatin A (P < 0.001). Antifungal drugs such as ketoconazole, terbinafine and amphotericin B had no, or only minor, inhibitory effects on proteolytic activity. In contrast, a significant reduction in Sap activity could be demonstrated during treatment with the antifungal agent ciclopiroxolamine (P < 0.001). These results point to a multiple effect of this antimycotic agent and might explain the reduced adherence of C. albicans to human epithelial cells at subinhibitory doses.

journal_name

J Med Microbiol

authors

Schaller M,Krnjaic N,Niewerth M,Hamm G,Hube B,Korting HC

doi

10.1099/jmm.0.05048-0

keywords:

subject

Has Abstract

pub_date

2003-03-01 00:00:00

pages

247-249

issue

Pt 3

eissn

0022-2615

issn

1473-5644

journal_volume

52

pub_type

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