Abstract:
:Invasive aspergillosis is a significant cause of morbidity and mortality within at-risk groups. Directed antifungal chemotherapy, guided by effective screening algorithms that incorporate reliable and validated molecular assays, reduces the morbidity associated with empirical administration and allows earlier diagnosis. The efficient extraction of nucleic acid from Aspergillus fumigatus is the main limiting factor for successful Aspergillus PCR from clinical specimens. With the integration of automated extraction platforms, assessment of the suitability of these platforms for specific targets is of paramount importance. In this study, four extraction robots (Applied Biosystems MagMAX, bioMérieux easyMAG, Qiagen EZ1 and Roche MagNA Pure LC) were evaluated for their ability to extract clinically significant levels of A. fumigatus from blood. All of the platforms could detect 10(1) c.f.u. ml(-1) from EDTA whole blood, although only the easyMAG, EZ1 and MagNA Pure had 100 % reproducibility at this level. Despite good analytical sensitivity, contamination associated with the easyMAG platform excluded its use for diagnostic Aspergillus PCR. The EZ1 and MagNA Pure platforms demonstrated equivalent high sensitivity and negative predictive values (97.4-100 %), essential for screening assays.
journal_name
J Med Microbioljournal_title
Journal of medical microbiologyauthors
Perry MD,White PL,Barnes RAdoi
10.1099/jmm.0.076315-0subject
Has Abstractpub_date
2014-09-01 00:00:00pages
1160-1166issue
Pt 9eissn
0022-2615issn
1473-5644journal_volume
63pub_type
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更新日期:2019-08-01 00:00:00
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更新日期:1998-07-01 00:00:00
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更新日期:2018-08-01 00:00:00
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doi:10.1099/jmm.0.034892-0
更新日期:2012-03-01 00:00:00
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更新日期:2008-01-01 00:00:00
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doi:10.1099/jmm.0.000581
更新日期:2017-10-01 00:00:00
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journal_title:Journal of medical microbiology
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doi:10.1099/jmm.0.45819-0
更新日期:2005-01-01 00:00:00
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journal_title:Journal of medical microbiology
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doi:10.1099/jmm.0.000311
更新日期:2016-09-01 00:00:00
abstract:PURPOSE:From May 2015 to March 2016, an outbreak due to Listeria monocytogenes serotype 1/2a and clinical pulsotype never previously isolated in Europe occurred in central Italy, involving 24 confirmed clinical cases. The article provides a description of the outbreak and the investigation carried out by a multidiscipl...
journal_title:Journal of medical microbiology
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doi:10.1099/jmm.0.000785
更新日期:2018-09-01 00:00:00
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journal_title:Journal of medical microbiology
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更新日期:2017-07-01 00:00:00
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更新日期:2010-01-01 00:00:00
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