Abstract:
:Clinical and bacteriological findings in seven cases of urinary tract infection with cysteine-requiring strains of Klebsiella are described. The organisms were isolated from patients with long-standing urinary tract abnormalities and grew as small (c. 1 mm) colonies on MacConkey agar. The organisms failed to grow in a minimal medium supplemented with sodium sulphate but grew when the medium was supplemented with cysteine sulphinic acid, sodium sulphide or L-cysteine. The smallest amount of cysteine required for optimal growth in a chemically defined medium was 20 mg/L. Cysteine-requiring strains of Escherichia coli had previously been shown to require a similar amount of cysteine and to be unable to reduce sulphate to sulphite; this suggests a common influence in the selection of cysteine auxotrophs in vivo. However, the amino acid inhibited the growth of E. coli at concentrations which only slightly altered growth of the Klebsiella strains. Problems with the isolation, identification and sensitivity testing of cysteine-requiring Klebsiella were also observed and methods by which these may be minimised are suggested.
journal_name
J Med Microbioljournal_title
Journal of medical microbiologyauthors
McIver CJ,Tapsall JWdoi
10.1099/00222615-26-3-211subject
Has Abstractpub_date
1988-07-01 00:00:00pages
211-5issue
3eissn
0022-2615issn
1473-5644journal_volume
26pub_type
杂志文章abstract::Two carbapenem-non-susceptible Klebsiella pneumoniae isolates, Z2554 and Z2110, were collected from a hospital in China and analysed by PFGE. K. pneumoniae Z2554 and Z2110 were genetically unrelated and showed resistance to ertapenem, and reduced susceptibility to imipenem and meropenem. Analysis of their beta-lactama...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.008094-0
更新日期:2009-09-01 00:00:00
abstract::Fifty-eight isolates of Shigella sonnei from three outbreaks in school children and eight control isolates from epidemiologically unrelated sporadic clinical infections in Taiwan were compared by antibiotic susceptibility testing and molecular typing. Antibiotic susceptibility testing showed that all strains except on...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/0022-1317-49-4-355
更新日期:2000-04-01 00:00:00
abstract::Clostridium difficile is the most common cause of antibiotic-associated diarrhoea. Antibiotics are presumed to disturb the normal intestinal microbiota, leading to depletion of the barrier effect and colonization by pathogenic bacteria. This first step of infection includes adherence to epithelial cells. We investigat...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.47676-0
更新日期:2008-06-01 00:00:00
abstract::The carriage on skin lesions, on healthy skin and in the nose, of micro-organisms that produce antibiotics active against Staphylococcus aureus has been studied. About 5% of sites (10% of all patients) yielded such organisms. No evidence could be found that the presence of producer organisms in skin lesions had a prot...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-13-2-329
更新日期:1980-05-01 00:00:00
abstract::Pulsed-field gel electrophoresis typing was performed on a retrospective set of 129 Moraxella catarrhalis isolates obtained over a 20 month period from 70 children admitted to, or presenting at, the Erasmus University Medical Center, Rotterdam, The Netherlands. The mean age of the children (at the end of the study) wa...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.05251-0
更新日期:2003-09-01 00:00:00
abstract::An outer-membrane protein (OMP) was isolated from a clinical strain of Bacteroides distasonis. Changes in growth media did not appreciably affect the appearance of this protein in crude outer-membrane preparations examined by SDS-PAGE. However, the proportion of the protein relative to other OMPs was greater in 24-h c...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-37-3-165
更新日期:1992-09-01 00:00:00
abstract::A novel combination of culturing and DNA-based terminal restriction fragment length polymorphism (TRFLP) analysis was used to investigate the effect of probiotics on antibiotic-induced gut microbiota alterations to determine if a probiotic preparation containing bifidobacteria and lactobacilli, taken during and after ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.47615-0
更新日期:2009-05-01 00:00:00
abstract::The family Adenoviridae consists of five genera of which the genus Mastadenovirus includes human viruses classified into 57 serotypes clustered into seven subgroups (A-G). Serotypes 40 and 41 (subgroup F) are specifically associated with childhood gastroenteritis and are the most common cause of acute gastroenteritis ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.072413-0
更新日期:2014-06-01 00:00:00
abstract::Corynebacterium jeikeium, a member of the non-diphtheria corynebacteria, has been rarely reported as being responsible for cardiovascular-device infection. Here, we report what is believed to be the first case of C. jeikeium pacemaker infection associated with the presence of proteinase-3 antineutrophil cytoplasmic an...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.023283-0
更新日期:2011-02-01 00:00:00
abstract::In a prospective study between February 2003 and June 2004, stool specimens of children less than 2 years of age with diarrhoea (n=218) and without diarrhoea (n=86), living in Vitória, Espírito Santo, Brazil, were examined for the presence of diarrhoeagenic Escherichia coli. E. coli isolates were tested by colony blot...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.47660-0
更新日期:2008-03-01 00:00:00
abstract::The toxic effects of the haemorrhagic toxin of Clostridium sporogenes were studied in mice, rats, guinea-pigs and rabbits, and in various cultured cells. In rabbits, but not in the other animals, intradermal injection with crude toxin and its injection into a ligated intestinal loop caused haemorrhage in both the skin...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-46-4-270
更新日期:1997-04-01 00:00:00
abstract::The aim of this retrospective study was to determine the clinical spectrum of group B streptococcal (GBS) bacteraemia in patients over 70 years old. Sixty-six adults with GBS bacteraemia were reviewed over a 5-year period. Disease characteristics, clinical diagnoses and underlying disease were compared in 33 older pat...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-47-7-649
更新日期:1998-07-01 00:00:00
abstract::In Mexico, there is a lack of up-to-date published data that show viruses to be the main cause of acute respiratory infection (ARI). The objective of this study was to estimate the comparative viral frequency between children under the age of 5 years with and without ARI (n = 179 in each group) in a suburban community...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.05007-0
更新日期:2003-07-01 00:00:00
abstract::A polymerase chain reaction (PCR) assay with oligonucleotide primers homologous to a portion of the urease C gene of Helicobacter pylori was evaluated for specificity with pure DNA and biopsy material. The assay was used to test for the presence of the organism in dental plaque. The species specificity of detection wa...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-39-5-338
更新日期:1993-11-01 00:00:00
abstract::Clostridium difficile is a frequent cause of severe, recurrent, post-antibiotic diarrhoea and pseudomembranous colitis. Its pathogenicity is mediated mainly by two toxins, TcdA and TcdB. However, different adhesins have also been described as important colonization factors which are implicated in the first step of the...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.029553-0
更新日期:2011-08-01 00:00:00
abstract::Recent molecular studies have led to the recognition of three distinct species within the Candida parapsilosis complex, namely Candida parapsilosis, Candida orthopsilosis and Candida metapsilosis. As currently available yeast identification systems fail to differentiate these species, there is a paucity of information...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.008235-0
更新日期:2009-06-01 00:00:00
abstract::The results of this study demonstrate that saline extracted surface-associated material (SAM) of Actinobacillus actinomycetemcomitans and Eikenella corrodens stimulates bone resorption at picomolar concentrations. Various inhibitors of known osteolytic mediators--indomethacin, interleukin-1 receptor antagonist (IL-1ra...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-41-3-197
更新日期:1994-09-01 00:00:00
abstract::Dermatophytes are a highly specialized group of keratinophilic and keratinolytic filamentous fungi causing a ringworm disease called dermatophytosis or superficial mycoses. Although dermatophyte infections do not threaten the host's life, they lower its quality in humans by causing discomfort related to cosmetic probl...
journal_title:Journal of medical microbiology
pub_type: 杂志文章,评审
doi:10.1099/jmm.0.000982
更新日期:2019-06-01 00:00:00
abstract::Five Bordetella pertussis strains of phase I were grown in conventional casamino-acid medium and in media modified by adding high concentrations of MgSO4 or nicotinic acid. Cells grown in high-magnesium media (in the C-mode) had only about 4% of the protective antigen (PA) and 6% of the histamine-sensitising factor (H...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-9-1-89
更新日期:1976-02-01 00:00:00
abstract::The distribution of hepatitis C virus (HCV) genotypes among Egyptian patients positive for anti-HCV was determined and their influence, when combined with neu-oncoprotein overexpression, on the development of hepatocellular carcinoma (HCC) was examined. The study groups included asymptomatic carriers (ASC) and patient...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/0022-1317-49-1-89
更新日期:2000-01-01 00:00:00
abstract::The possession of any of eight different plasmids by Staphylococcus aureus strain 649--either singly or simultaneously (in no. 649MR)--caused changes in growth kinetics. Six of the plasmids caused an increase in exponential doubling time (by 8-25%), and most also altered the duration of the lag period. Strain 649MR wa...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-8-1-137
更新日期:1975-02-01 00:00:00
abstract::The acid end-products of 185 isolates from the family Bacteroidaceae were separated and analysed by gas-liquid chromatography on broth cultures. Different media were evaluated and definitive studies were performed in a fully supplemented complex medium. The limitations of this approach to the identification of a wide ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-11-2-81
更新日期:1978-05-01 00:00:00
abstract::The acute dental abscess is frequently underestimated in terms of its morbidity and mortality. The risk of potential serious consequences arising from the spread of a dental abscess is still relevant today with many hospital admissions for dental sepsis. The acute dental abscess is usually polymicrobial comprising fac...
journal_title:Journal of medical microbiology
pub_type: 杂志文章,评审
doi:10.1099/jmm.0.003517-0
更新日期:2009-02-01 00:00:00
abstract::Hepatitis E virus (HEV) is an RNA virus that is an important cause of both acute and chronic hepatitis worldwide. To date, there are eight HEV genotypes that can infect mammals. HEV-1 and HEV-2 infect exclusively humans, while HEV-3 and HEV-4 infect humans and various animals, mainly pigs and deer. Additionally, two n...
journal_title:Journal of medical microbiology
pub_type: 杂志文章,评审
doi:10.1099/jmm.0.000706
更新日期:2018-04-01 00:00:00
abstract::Escherichia coli isolates (n=2629) were collected between 1996 and 2000 from 2100 Thai children less than 12 years of age with acute diarrhoea. Enterotoxigenic (ETEC), enteroinvasive (EIEC), Shiga-toxin-producing (STEC), enteropathogenic (EPEC) and enteroaggregative (EAEC) E. coli were identified by their virulence ma...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.05413-0
更新日期:2004-03-01 00:00:00
abstract::The use of alkaline phosphatase fusion methodology to identify Helicobacter pylori exported proteins enabled the identification of an open reading frame (ORF) encoding a highly immunogenic, previously uncharacterised exported protein. The predicted aminoacid sequence displays a typical N-terminal signal peptide and co...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/0022-1317-49-7-643
更新日期:2000-07-01 00:00:00
abstract::Sequence variations located at the signal sequence and mid-region within the vacA gene, the 3'-end of the cagA gene, the indel motifs at the 3'-end of the cag pathogenicity island and the regions upstream of the vacA and ribA genes were determined by PCR in 19 paired antral or antrum and corpus Helicobacter pylori iso...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.05440-0
更新日期:2004-07-01 00:00:00
abstract::The family of leptospiral immunoglobulin-like (lig) genes comprises ligA, ligB and ligC. This study used PCR to demonstrate the presence of lig genes among serovars from a collection of leptospiral strains and clinical isolates. Whilst ligA and ligC appeared to be present in a limited number of pathogenic serovars, th...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.009175-0
更新日期:2009-09-01 00:00:00
abstract::Pseudomonas aeruginosa is an opportunistic pathogen that poses a threat in clinical settings due to its intrinsic and acquired resistance to a wide spectrum of antibiotics. Additionally, the presence of a subpopulation of cells surviving high concentrations of antibiotics, called persisters, makes it virtually impossi...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.019703-0
更新日期:2011-03-01 00:00:00
abstract::Cell-surface hydrophobicity of Helicobacter (formerly Campylobacter) pylori was tested by aqueous two-phase partitioning and hydrophobic interaction chromatography. The hydrophobicity of H. pylori greatly exceeded that of Campylobacter fetus subsp. fetus, C. jejuni and Bacillus subtilis. A partition coefficient (PC) o...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-32-2-93
更新日期:1990-06-01 00:00:00