Abstract:
:Fifty-eight isolates of Shigella sonnei from three outbreaks in school children and eight control isolates from epidemiologically unrelated sporadic clinical infections in Taiwan were compared by antibiotic susceptibility testing and molecular typing. Antibiotic susceptibility testing showed that all strains except one sporadic isolate were multi-resistant. Ribotyping after restriction endonuclease digestion with SalI, PvuII and HindII generated the same ribosomal pattern in 65 of the 66 isolates. Plasmid profile analysis and pulsed-field gel electrophoresis (PFGE) produced eight and nine distinct patterns, respectively, and were in agreement with the epidemiological relationship of the outbreak strains. Nevertheless, some of the sporadic isolates could be discriminated only by a combination of these two methods. This study showed that plasmid profiling in combination with PFGE may be superior to ribotyping in molecular epidemiological investigations of S. sonnei.
journal_name
J Med Microbioljournal_title
Journal of medical microbiologyauthors
Lee TM,Chang LL,Chang CY,Wang JC,Pan TM,Wang TK,Chang SFdoi
10.1099/0022-1317-49-4-355keywords:
subject
Has Abstractpub_date
2000-04-01 00:00:00pages
355-360issue
4eissn
0022-2615issn
1473-5644journal_volume
49pub_type
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