Abstract：
:Human gastric epithelial immortalized GES-1 cells were infected with spiral and coccoid Helicobacter pylori. Scanning electron microscopy was used to determine the ability of the two forms of H. pylori to adhere to GES-1 cells. GES-1 cell apoptosis induced by coccoid and spiral H. pylori was analysed using flow cytometry. A cDNA microarray for 22,000 human genes was used to identify the gene-expression differences in GES-1 cells infected with the two forms of H. pylori, and the gene expression identified by the cDNA microarray was confirmed by RT-PCR. Scanning electron microscope observation showed that both coccoid and spiral bacteria can adhere to GES-1 cells. After 4 h infection, apoptosis induction was 27.4% for spiral-form infection and 10.2% for coccoid-form infection. Of 268 differentially expressed genes identified by cDNA microarray, 166 showed higher expression with the spiral H. pylori infection than with the coccoid H. pylori infection. To the best of the authors' knowledge, this is the first report that GES-1 cells infected with spiral H. pylori have higher expression of cxcl10, ccl11, ccl5, groalpha, TLR5, ATF3, fos, fosl2, gadd45a and myc. The cells infected with coccoid H. pylori had higher expression of survivin. The global profile of gene expression in GES-1 cells infected with coccoid and spiral H. pylori is described for the first time.
journal_name
J Med Microbioljournal_title
Journal of medical microbiologyauthors
Liu ZF,Chen CY,Tang W,Zhang JY,Gong YQ,Jia JHdoi
10.1099/jmm.0.46456-0subject
Has Abstractpub_date
2006-08-01 00:00:00pages
1009-1015issue
Pt 8eissn
0022-2615issn
1473-5644journal_volume
55pub_type
杂志文章abstract：:Human granulocytic anaplasmosis (HGA) and Lyme borreliosis (LB) are tick-borne infectious diseases caused by Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato species, respectively. In this study, p44/msp2 paralogues specific to A. phagocytophilum and 5S-23S rRNA gene-intergenic spacers specific to B. burg...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.47721-0
更新日期：2008-08-01 00:00:00
abstract：:To study the influence of normal associated microbiota on systemic immunological responses during experimental Chagas' disease, germ-free and conventional NIH Swiss mice were infected with Y strain of Trypanosoma cruzi. Although no statistical differences in mortality and parasitaemia were found, conventional mice sho...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.45657-0
更新日期：2004-08-01 00:00:00
abstract：:A colony hybridisation method with enzyme-labelled oligonucleotide probes was developed to detect the gene for toxic shock syndrome toxin 1 (tst). For rapid identification, bacterial colonies were transferred from agar plates directly on to nylon membranes. These procedures took only 3 h. Results obtained by this test...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-44-3-215
更新日期：1996-03-01 00:00:00
abstract：:Anaplasma phagocytophilum, an agent of human granulocytic anaplasmosis, infects neutrophils and causes an emerging tickborne febrile disease. The genome of this bacterium contains a large number of p44/msp2-related genes encoding 44 kDa major outer-membrane proteins, and it is known that a specific p44/msp2 gene is pr...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.011775-0
更新日期：2009-12-01 00:00:00
abstract：:Entero-invasive Escherichia coli (EIEC) and shigellae were tested for contact-haemolysin (CH) with red blood cells (RBCs) of guinea-pig, rabbit, rat, mouse, monkey, man, sheep and chicken; all bacteria showed the best lysis with guinea-pig RBCs. The best culture medium for CH activity of shigellae was tryptic soy brot...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-35-6-330
更新日期：1991-12-01 00:00:00
abstract：:The heparin-binding properties of six different species of coagulase-negative staphylococci were examined by a particle agglutination assay. Heparin (mol. wt 4000-6000), mildly treated with sodium periodate, was covalently coupled to amino-modified latex beads (0.72 micron diameter). The particle agglutination assay w...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-45-4-263
更新日期：1996-10-01 00:00:00
abstract：:Enterovirus 71 (EV71) and coxsackievirus A16 (CVA16) are members of the species Human enterovirus A, and are both major and independent aetiological agents of hand-foot-and-mouth disease. The human enterovirus (HEV) 5' untranslated region (UTR) is fundamentally important for efficient virus replication and for virulen...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.025056-0
更新日期：2011-03-01 00:00:00
abstract：:A collection of 820 isolates of streptococci and aerococci from human blood or internal organs was classified by means of a set of cultural and biochemical tests. Of these, 719 (712 streptococci and 7 aerococci) were from patients believed to be suffering from a systemic infection: endocarditis 317; a purulent lesion ...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-9-3-275
更新日期：1976-08-01 00:00:00
abstract：:A total of 110 clinical isolates of Vibrio cholerae O1 biotype El Tor serotype Ogawa isolated in a recent outbreak from different districts of Teheran, Iran, was subjected to 99 carbon source utilisation tests, ribotyping and toxinogenotyping. PCR showed that the genes encoding cholera toxin (ctxA), toxin co-regulated...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/0022-1317-49-12-1085
更新日期：2000-12-01 00:00:00
abstract：:Forty carbapenem-resistant Klebsiella pneumoniae isolates were recovered from 28 patients from various sites in an intensive care unit in Zhejiang Provincial People's Hospital, China, over a 6 month period. PFGE analysis indicated that the 40 strains were all closely related. The MICs of carbapenems varied from 16 to ...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.015826-0
更新日期：2011-07-01 00:00:00
abstract：:A collection of 198 clinical isolates of strains belonging to the tribe Proteeae was examined for haemolytic activity on blood agar and in Brain Heart Infusion Broth. The strains were of diverse bacteriocin and O-antigenic types and from a wide variety of sources. They included representatives of all species of Morgan...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-25-1-17
更新日期：1988-01-01 00:00:00
abstract：:Three strains of Plesiomonas shigelloides isolated from patients with diarrhoea were agglutinated with Shigella flexneri 6 antiserum in slide and tube tests. All the strains were also agglutinated with a monoclonal antibody to the common group 1 antigen shared between S. flexneri serotypes and S. dysenteriae type 1. F...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-39-3-211
更新日期：1993-09-01 00:00:00
abstract：:The prevalence of chlamydial DNA determined by PCR and in-situ hybridisation (ISH) in fresh tissue specimens (endometrium, fallopian tube and ovary) was investigated in 33 women presenting with ectopic pregnancy (EP), 14 women with tubal factor infertility (TFI) and 50 control patients from the UK and the West Indies....
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/0022-1317-50-10-902
更新日期：2001-10-01 00:00:00
abstract：:The Gen-Probe APTIMA Combo 2 assay has previously been reported to have a high sensitivity. The end point of this assay was evaluated using highly purified chlamydial elementary bodies (EBs). The performance of the APTIMA Combo 2 assay was compared with a commercially available PCR kit, AMPLICOR Chlamydia trachomatis....
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.45911-0
更新日期：2005-04-01 00:00:00
abstract：:Antibiotic resistance in Staphylococcus aureus is a major public health concern, and methicillin-resistant S. aureus has emerged as an important pathogen. We characterized S. aureus isolates from monomicrobial and polymicrobial wound infections from 200 diabetic individuals with foot ulcers to understand their underly...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.000370
更新日期：2016-12-01 00:00:00
abstract：:Simulated throat swabs were prepared with known numbers of Streptococcus pyogenes; some were suspended in pasteurised human saliva and an equal number in saline. Two types of commercially available swabs were tested; these were composed of (1) plain, buffered, cotton wool, and (2) albumen-coated cotton wool. The mean ...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-10-1-69
更新日期：1977-02-01 00:00:00
abstract：:Human herpesvirus 6 (HHV-6) exists as distinct variants HHV-6A and HHV-6B. The complete genomes of HHV-6A and HHV-6B have been sequenced. HHV-6B contains 97 unique genes. CD46 is the cell receptor for HHV-6, explaining its broad tissue tropism but its restricted host-species range. HHV-6 utilizes a number of strategie...
journal_title：Journal of medical microbiology
pub_type： 杂志文章,评审
doi：10.1099/jmm.0.05074-0
更新日期：2003-01-01 00:00:00
abstract：:Recent studies have shown that the intestinal microbiota is essential for the pathogenicity but not for the multiplication of Giardia duodenalis in the intestinal lumen. The microbial components responsible for this phenomenon are not known. Twenty-eight facultative and three strictly anaerobic micro-organisms were is...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/0022-1317-49-3-209
更新日期：2000-03-01 00:00:00
abstract：:The distribution of cryptic plasmids among 123 isolates of Providencia stuarti from a hospital ward during a prospective epidemiological study is reported. Two closely related stable plasmids (34 Kb and 36 Kb) were identified by restriction endonuclease digest analysis of plasmid DNA. One or other of these cryptic pla...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-18-2-277
更新日期：1984-10-01 00:00:00
abstract：:We report a case of septicaemia with prolonged, refractory hypotension related to Moraxella osloensis isolated in a non-immunocompromised paediatric patient. ...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.016378-0
更新日期：2011-01-01 00:00:00
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journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.015008-0
更新日期：2010-03-01 00:00:00
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journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-38-2-145
更新日期：1993-02-01 00:00:00
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journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.47615-0
更新日期：2009-05-01 00:00:00
abstract：:This study used a previously described multiplex PCR-based reverse line blot (mPCR/RLB) assay to assess the prevalence and distribution of 14 urogenital pathogens or putative pathogens, namely Neisseria gonorrhoeae, Chlamydia trachomatis, Mycoplasma genitalium, Mycoplasma hominis, Trichomonas vaginalis, Gardnerella va...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.029108-0
更新日期：2011-07-01 00:00:00
abstract：:Infections of the parotid gland with non-tuberculous mycobacteria (NTM) are rarely described. Here, we report on an infection of the parotid gland caused by Mycobacterium avium and give a literature-based overview about this entity. In the light of a global increase of mycobacterial infections, unusual manifestations ...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.47698-0
更新日期：2008-04-01 00:00:00
abstract：:Nosocomial infections with enterococci are an increasing problem in modern medical practice due to the development of resistance to a wide range of antibiotics, including the glycopeptides vancomycin and teicoplanin. An increasing number of vancomycin-resistant enterococci (VRE) have been cultured from clinical specim...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-48-10-935
更新日期：1999-10-01 00:00:00
abstract：:Yokenella regensburgei is an opportunistic human pathogen of the Enterobacteriaceae family rarely reported to cause human infections. Here, we present a case report of Y. regensburgei bacteraemia from India clinically resembling enteric fever in an apparently immunocompetent paediatric patient. ...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.058669-0
更新日期：2013-06-01 00:00:00
abstract：:A detailed study was made of lambs aged 5--7 months naturally infected with Pasteurella haemolytica biotype T. In addition to the well known features of such infections, previously unreported necrotic lesions of the tonsil, oesophagus, pharynx and adjacent areas were consistently seen. Large numbers of P. haemolytica ...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-14-1-89
更新日期：1981-02-01 00:00:00
abstract：:Resistance to desiccation and to skin fatty acids was measured in three groups of methicillin-resistant Staphylococcus aureus (MRSA) strains and a group of control strains. Organisms from a large outbreak on a special care baby unit (SCBU), where MRSA had been isolated from staff hands but not from the environment, we...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-36-1-56
更新日期：1992-01-01 00:00:00
abstract：:Prairie dogs (Cynomys ludovicianus) are used to study the aetiology and prevention of gallstones because of the similarities of prairie dog and human bile gallstone composition. Epidemiological and experimental studies have suggested a connection between infection with Helicobacter species and cholesterol cholelithias...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.032144-0
更新日期：2011-09-01 00:00:00
