In vitro assessment of the APTIMA Combo 2 assay for the detection of Chlamydia trachomatis using highly purified elementary bodies.

abstract：:A total of 118 meticillin-resistant Staphylococcus aureus (MRSA) and 140 meticillin-susceptible S. aureus (MSSA) isolates from different patients in the same time period were comprehensively searched using a multiplex PCR for the classical and recently described superantigenic toxin gene family comprising the staphylo...

journal_title：Journal of medical microbiology

authors： Hu DL,Omoe K,Inoue F,Kasai T,Yasujima M,Shinagawa K,Nakane A

更新日期：2008-09-01 00:00:00

abstract：:Mice immunised by the oral or intraperitoneal route with a live aromatic-dependent strain of Salmonella typhimurium exhibited significantly less protection against oral challenge with 50 LD50 of an ovine isolate of S. typhimurium (12313) than when a bovine isolate with the same O antigens and phage-type as strain 1231...

journal_title：Journal of medical microbiology

authors： Mukkur TK,McDowell GH,Stocker BA,Lascelles AK

更新日期：1987-08-01 00:00:00

abstract：:The lipopolysaccharides (LPS) of the 10 species of the genus Bacteroides (sensu stricto) were extracted by the proteinase K method and their resolution compared by several methods of polyacrylamide gel electrophoresis (PAGE). These included sodium dodecyl sulphate (SDS)-PAGE with and without urea, polyacrylamide gradi...

journal_title：Journal of medical microbiology

authors： Maskell JP

更新日期：1991-05-01 00:00:00

abstract：:The heparin-binding properties of six different species of coagulase-negative staphylococci were examined by a particle agglutination assay. Heparin (mol. wt 4000-6000), mildly treated with sodium periodate, was covalently coupled to amino-modified latex beads (0.72 micron diameter). The particle agglutination assay w...

journal_title：Journal of medical microbiology

authors： Pascu C,Hirmo S,Ljungh A,Wadström T

更新日期：1996-10-01 00:00:00

abstract：:Sixty-one isolates of Candida albicans were tested for their phospholipase activity and this parameter was correlated with pathogenicity in albino mice. Of the isolates tested, 57 (93%) showed appreciable phospholipase activity and of these, 55 (90%) were pathogenic to mice. A significant correlation was found between...

journal_title：Journal of medical microbiology

authors： Kothavade RJ,Panthaki MH

更新日期：1998-02-01 00:00:00

abstract：:The distribution of K1, K5 and O antigens was examined in 500 clinical strains of Escherichia coli. Of 400 strains from urine, 52% belonged to serogroups O1, O2, O4, O6, O7, O8, O9, O18, O25 and O75; 34% were non-typable (NT) and 14% were autoagglutinable (AA). Antigen K1 was carried by 17% of these strains, and K5 by...

journal_title：Journal of medical microbiology

authors： Devine DA,Robinson L,Roberts AP

更新日期：1989-12-01 00:00:00

abstract：:Many clinically relevant biofilms are polymicrobial. Examining the effect of antimicrobials in a multispecies biofilm consortium is of great clinical importance. The goal of this study was to investigate the effect of different honey types against bacterial wound pathogens grown in multispecies biofilm and to test the...

journal_title：Journal of medical microbiology

authors： Sojka M,Valachova I,Bucekova M,Majtan J

更新日期：2016-04-01 00:00:00

abstract：:Immunodiffusion analysis of Mycobacterium lepraemurium indicated the presence of at lease six antigens. Comparative analysis of the M. lepraemurium antigen-antibody system with similar systems established for other mycobacterial species, showed that M. lepraemurium shared up to two antigens with other species. Althoug...

journal_title：Journal of medical microbiology

authors： Navalkar RG,Dalvi RR,Patel PJ

更新日期：1975-02-01 00:00:00

abstract：:Experiments were performed to determine whether a modern flow cytometer could be used to study bacterial populations in suspension, with particular reference to their morphological characteristics and their responses to antibiotics. The FACScan, a commercial benchtop flow cytometer fitted with an air-cooled laser, des...

journal_title：Journal of medical microbiology

authors： Gant VA,Warnes G,Phillips I,Savidge GF

更新日期：1993-08-01 00:00:00

abstract：:Early diagnosis of leptospirosis is important because severe leptospiral infection can run a fulminant course. The polymerase chain reaction (PCR) was evaluated for the detection of leptospires in clinical samples from patients with acute leptospiral infection. Blood and urine samples from 71 patients with leptospiros...

journal_title：Journal of medical microbiology

authors： Brown PD,Gravekamp C,Carrington DG,van de Kemp H,Hartskeerl RA,Edwards CN,Everard CO,Terpstra WJ,Levett PN

更新日期：1995-08-01 00:00:00

abstract：:Detection of latent Mycobacterium tuberculosis is a challenge in the diagnosis of asymptomatic, subclinical tuberculosis. We report the development of an immunofluorescence technique to visualize and enumerate M. tuberculosis in latently infected rabbit lungs where no acid-fast-stained organisms were seen and no culti...

journal_title：Journal of medical microbiology

authors： Subbian S,Eugenin E,Kaplan G

更新日期：2014-11-01 00:00:00

abstract：:Thirty-seven multidrug-resistant and 13 pan-susceptible isolates of Mycobacterium tuberculosis were analysed for the diversity of genotypes associated with known drug-resistance mechanisms. The isolates were obtained from patients attending a university tuberculosis clinic in Monterrey, Mexico. A total of 25 IS6110-RF...

journal_title：Journal of medical microbiology

authors： Ramaswamy SV,Dou SJ,Rendon A,Yang Z,Cave MD,Graviss EA

更新日期：2004-02-01 00:00:00

abstract：:To clarify further the role of chemotaxis in Helicobacter pylori colonization, the in vitro bacterium response to human plasma and bile (secretions containing chemoeffector compounds that are present in the gastric mucus layer) was examined. Human plasma, after dilution to 1 % (v/v) with buffer, was found to be a chem...

journal_title：Journal of medical microbiology

authors： Worku ML,Karim QN,Spencer J,Sidebotham RL

更新日期：2004-08-01 00:00:00

abstract：:Nasal polyp cells in primary culture from cystic fibrosis (CF) and non-CF patients were compared for the ability to bind Pseudomonas aeruginosa cells and for the presence of sulphated glycoconjugates at the epithelial cell surface. Quantitation of bacterial adhesion, by scanning electronmicroscopy, showed no significa...

journal_title：Journal of medical microbiology

authors： Plotkowski MC,Chevillard M,Pierrot D,Altemayer D,Puchelle E

更新日期：1992-02-01 00:00:00

abstract：:The purpose of this study was to determine optimal criteria for microbiology laboratory screening of endotracheal tube (ETT) specimens submitted for bacterial culture from adult patients. ETT specimens from adult patients that were received by two microbiology laboratories were prospectively evaluated and subdivided i...

journal_title：Journal of medical microbiology

authors： Walkty A,Lagacé-Wiens PRS,Manickam K,Adam H,Pieroni P,Alfa M,Karlowsky JA

更新日期：2012-09-01 00:00:00

abstract：:In order to develop a model for the study of gastric spiral bacteria, and based on the observation that Wistar rats do not carry urease-positive spiral bacteria in their gastric mucosa, mucus from a pig naturally colonised by 'Gastrospirillum suis' (an organism with I6S rDNA 99.5% similar to that of 'G. hominis' type ...

journal_title：Journal of medical microbiology

authors： Mendes EN,Queiroz DM,Coimbra RS,Moura SB,Barbosa AJ,Rocha GA

更新日期：1996-02-01 00:00:00

abstract：:Production of a meningococcal vaccine capable of generating long-lasting immunity in all age groups is still a high priority worldwide. Iron-regulated outer-membrane proteins have attracted considerable attention in recent years and it has become increasingly evident that the meningococcal transferrin-binding proteins...

journal_title：Journal of medical microbiology

authors： Ala'Aldeen DA

更新日期：1996-04-01 00:00:00

abstract：:Cryptococcosis causes disseminated disease in AIDS patients. In contrast to what occurs in laboratory conditions, a large capsule is produced by Cryptococcus neoformans in vivo during infection. The aim of this study was to compare the in vitro activity of different antifungal agents against 34 clinical isolates of C....

journal_title：Journal of medical microbiology

authors： Vitale RG,Pascuccelli V,Afeltra J

更新日期：2012-03-01 00:00:00

abstract：:Acinetobacter baumannii is an opportunist nosocomial pathogen of world-wide importance and produces severe infections in immunocompromised patients. However, the virulence factors contributing to its pathogenic properties are not well known. The effect of normal human serum against 18 clinical isolates of the most pre...

journal_title：Journal of medical microbiology

authors： García A,Solar H,González C,Zemelman R

更新日期：2000-11-01 00:00:00

abstract：:Community-associated meticillin-resistant Staphylococcus aureus (CA-MRSA) have emerged globally and have been associated with more severe disease than healthcare-associated MRSA (HA-MRSA). The purpose of this study was to determine whether laboratory measures of virulence can distinguish dominant CA-MRSA clones from H...

journal_title：Journal of medical microbiology

authors： Boakes E,Marbach H,Lynham S,Ward M,Edgeworth JD,Otter JA

更新日期：2016-12-01 00:00:00

abstract：:The response of Legionella pneumophila to antibiotics that inhibit cell-wall, protein and DNA synthesis was examined by electronmicroscopy, MIC estimations and viable counts. Ampicillin, cefotaxime, methicillin, erythromycin, rifampicin and ciprofloxacin, each used separately at 20 times their respective MIC values, s...

journal_title：Journal of medical microbiology

authors： Rodgers FG,Tzianabos AO,Elliott TS

更新日期：1990-01-01 00:00:00

abstract：:Sequence variations located at the signal sequence and mid-region within the vacA gene, the 3'-end of the cagA gene, the indel motifs at the 3'-end of the cag pathogenicity island and the regions upstream of the vacA and ribA genes were determined by PCR in 19 paired antral or antrum and corpus Helicobacter pylori iso...

journal_title：Journal of medical microbiology

authors： Carroll IM,Ahmed N,Beesley SM,Khan AA,Ghousunnissa S,Moráin CAÓ,Habibullah CM,Smyth CJ

更新日期：2004-07-01 00:00:00

abstract：:The determinant responsible for the ability of Bacteroides spp. to inhibit polymorph phagocytic killing of aerobic organisms has not yet been identified. Therefore, the roles of lipopolysaccharide and capsular polysaccharide of B. fragilis were investigated. Serum-resistant and serum-sensitive strains of Proteus mirab...

journal_title：Journal of medical microbiology

authors： Connolly JC,McLean C,Tabaqchali S

更新日期：1984-06-01 00:00:00

abstract：:The growth of five species of Bacteroides in four anaerobic culture media was continuously monitored turbidimetrically. Interspecies differences were observed in the growth of Bacteroides spp. in the various media, but growth in Brain Heart Infusion broth supplemented with yeast extract, haemin and menadione, was cons...

journal_title：Journal of medical microbiology

authors： Eley A,Greenwood D,O'Grady F

更新日期：1985-04-01 00:00:00

abstract：:The antifungal susceptibilities of 598 isolates of Candida spp. (bloodstream and other sterile sites) to liposomal amphotericin B (L-AmB) versus amphotericin B (AmB) were determined. MICs were calculated using the Clinical and Laboratory Standards Institute broth microdilution (M27-A3) method for L-AmB and the Etest m...

journal_title：Journal of medical microbiology

authors： Lovero G,Giglio O,Rutigliano S,Diella G,Caggiano G,Montagna MT

更新日期：2017-03-01 00:00:00

abstract：:Helicobacter pylori infection represents a key factor in the aetiology of various gastrointestinal diseases. H. pylori infection diagnosis is generally achieved using both invasive (e.g. biopsy of the gastric epithelium) and non-invasive methods. Therefore, cultivation on a growth medium becomes complex. Trypsin is a ...

journal_title：Journal of medical microbiology

authors： Peretz A,Paritsky M,Pastukh N,Koifman A,Brodsky D,Glyatman T,On A

更新日期：2015-06-01 00:00:00

abstract：:Introduction. PCV2 is a DNA virus that exists widely in pigs and has caused great economic losses to the pig industry worldwide. In the existing commercial PCV2 enzyme-linked immunosorbent assay (ELISA) kits both natural infection with PCV2 and vaccine immunization produce results that are positive for PCV2 Cap antibo...

journal_title：Journal of medical microbiology

authors： Chen Q,Rong J,Li G,Xu B,Wang X,Hu J,Rong M,Li H

更新日期：2020-09-01 00:00:00

abstract：:The characterization of integrons and their promoters in 156 antibiotic-resistant clinical isolates of the important zoonotic pathogens Salmonella enterica subsp. enterica serovars Typhimurium (S. Typhimurium) and Enteritidis (S. Enteritidis) from Norwegian hospitals were performed. Integrons were found in 64 of 66 S....

journal_title：Journal of medical microbiology

authors： Lindstedt BA,Heir E,Nygård I,Kapperud G

更新日期：2003-02-01 00:00:00

abstract：:The purpose of this study was to investigate the prevalence and epidemiology of the anaerobic intestinal spirochaete Brachyspira pilosicoli amongst Indonesians living in rural and urban settings on the island of Bali. Faecal samples (n = 992) were collected on two occasions, 4 months apart, from people living in four ...

journal_title：Journal of medical microbiology

authors： Margawani KR,Robertson ID,Brooke CJ,Hampson DJ

更新日期：2004-04-01 00:00:00

abstract：:Oral biofilms are one of the greatest challenges in dental research. The present study aimed to investigate initial bacterial colonization of enamel surfaces in situ using fluorescence in situ hybridization (FISH) over a 12 h period. For this purpose, bovine enamel slabs were fixed on buccal sites of individual splint...

journal_title：Journal of medical microbiology

authors： Al-Ahmad A,Follo M,Selzer AC,Hellwig E,Hannig M,Hannig C

更新日期：2009-10-01 00:00:00