The isolation of Streptococcus pyogenes from throat swabs.

Abstract:

:Simulated throat swabs were prepared with known numbers of Streptococcus pyogenes; some were suspended in pasteurised human saliva and an equal number in saline. Two types of commercially available swabs were tested; these were composed of (1) plain, buffered, cotton wool, and (2) albumen-coated cotton wool. The mean recovery rates of first platings on solid media from albumen-coated and plain cotton-wool swabs were similar (8-2% and 8-3%) and the mean recovery rates from platings 1-4 were also similar (6-6% and 6-5%). The greater the delay in plating, the less were the chances of recovery of streptococci, although the viability of these was significantly prolonged on swabs held at 4 degrees C. Similar results were again produced by both types of swabs; processing swabs in saliva, however, produced by both types of swabs; processing swabs in saliva, however, produced a recovery rate that was 1-2% greater than the rate for the saline series. Swabs were also agitated or squeezed by forceps in nutrient broth to release any organisms they contained, and standard samples of the broth were then plated on solid media. Counts thus obtained indicated that about 50% of the original inoculum was still viable and could be recovered from the broth; in comparison, recoveries from initial direct plating of the swabs were low. No demonstrable toxic effect was produced by the cotton wool in these experiments.

journal_name

J Med Microbiol

authors

Ross PW

doi

10.1099/00222615-10-1-69

subject

Has Abstract

pub_date

1977-02-01 00:00:00

pages

69-76

issue

1

eissn

0022-2615

issn

1473-5644

journal_volume

10

pub_type

杂志文章
  • Incidence of common pyocin types of Pseudomonas aeruginosa from patients with cystic fibrosis and chronic airways diseases.

    abstract::We sought evidence to determine if particular strains of Pseudomonas aeruginosa have a predilection for pulmonary colonisation in patients with cystic fibrosis (CF). The incidence of common pyocin types in non-CF isolates (74%) was similar to that noted in previous reports but differed significantly (X2 = 16.7, p less...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-32-3-169

    authors: Tredgett MW,Doherty C,Govan JR

    更新日期:1990-07-01 00:00:00

  • Nasal colonization by four potential respiratory bacteria in healthy children attending kindergarten or elementary school in Seoul, Korea.

    abstract::A longitudinal analysis was carried out of the colonization by four potential respiratory pathogens - Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis and Staphylococcus aureus - in 165 healthy children (aged 3-7 years) attending three kindergartens and 417 healthy children (aged 7-10 years) att...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.040584-0

    authors: Bae S,Yu JY,Lee K,Lee S,Park B,Kang Y

    更新日期:2012-05-01 00:00:00

  • Sepsis with prolonged hypotension due to Moraxella osloensis in a non-immunocompromised child.

    abstract::We report a case of septicaemia with prolonged, refractory hypotension related to Moraxella osloensis isolated in a non-immunocompromised paediatric patient. ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.016378-0

    authors: Dien Bard J,Lewinski M,Summanen PH,Deville JG

    更新日期:2011-01-01 00:00:00

  • Lemierre's syndrome and septicaemia caused solely by Arcanobacterium haemolyticum in a young immunocompetent patient.

    abstract::We present a case of septicaemia caused by Arcanobacterium haemolyticum in a previously healthy 23-year-old man suffering from acute pharyngotonsillitis, who developed complicated Lemierre's syndrome. Three blood cultures (both aerobic and anaerobic) revealed the exclusive presence of A. haemolyticum. The presence of ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.007492-0

    authors: Fernández-Suárez A,Aguilar Benítez JM,López Vidal AM,Díaz Iglesias JM

    更新日期:2009-12-01 00:00:00

  • The role of alpha haemolysin in the virulence of Escherichia coli for mice.

    abstract::Transfer of an Hly plasmid determining production of alpha haemolysin to a non-haemolytic strain of Escherichia coli increased the virulence of the strain for mice. Injections of non-toxic amounts of alpha haemolysin, phenylhydrazine, haemoglobin, iron or manganese salts simulated the effect of the Hly plasmid by stim...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-15-1-23

    authors: Linggood MA,Ingram PL

    更新日期:1982-02-01 00:00:00

  • Molecular analysis of Streptococcus pneumoniae clones causing invasive disease in children in Singapore.

    abstract::Streptococcus pneumoniae remains a leading cause of serious paediatric disease. However, there are few published epidemiological data regarding invasive pneumococcal disease (IPD) in many countries in South East Asia, including Singapore. Baseline data for IPD are essential to inform policy regarding pneumococcal conj...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.030007-0

    authors: Jefferies JMC,Tee WSN,Clarke SC

    更新日期:2011-06-01 00:00:00

  • A comparison of chloramphenicol and ampicillin as bactericidal agents for Haemophilus influenzae type B.

    abstract::In tests of bactericidal action against H. influenzae type b strains isolated from patients with meningitis, chloramphenicol was found to be far more reliable than ampicillin in dealing with large inocula, and more rapidly effective against both large and relatively small inocula. These findings provide a laboratory e...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-10-1-127

    authors: Turk DC

    更新日期:1977-02-01 00:00:00

  • Cronobacter sakazakii induces necrotizing enterocolitis by regulating NLRP3 inflammasome expression via TLR4.

    abstract::Introduction. Neonatal infection with Cronobacter sakazakii can cause severe intestinal damage and necrotizing enterocolitis (NEC). The inflammasome and Toll-like receptors mediate intestinal damage caused by other intestinal pathogens causing NEC, but the exact mechanism is unclear.Aim. We evaluated the molecular mec...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.001181

    authors: Chen Z,Zhang Y,Lin R,Meng X,Zhao W,Shen W,Fan H

    更新日期:2020-05-01 00:00:00

  • Isolation of Carnobacterium sp. from a human blood culture.

    abstract::Carnobacterium species have been isolated from the environment and are not regarded as human pathogens, although they are known to cause disease in fish. Only two reports describing isolation of Carnobacterium species from human pus were found in the literature. We report what we believe to be the first isolation of C...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.016808-0

    authors: Hoenigl M,Grisold AJ,Valentin T,Leitner E,Zarfel G,Renner H,Krause R

    更新日期:2010-04-01 00:00:00

  • Pleuritis due to Brevundimonas diminuta in a previously healthy man.

    abstract::Brevundimonas diminuta is rarely associated with invasive infections. We report the case of a previously healthy young man with pleural effusion, in which B. diminuta was recovered but incorrectly identified as Kingella kingae when it was freshly isolated. Consequently, the misidentification resulted in initial treatm...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.045013-0

    authors: Lu B,Shi Y,Zhu F,Xu X

    更新日期:2013-03-01 00:00:00

  • Laboratory diagnosis of Clostridium perfringens antibiotic-associated diarrhoea.

    abstract::Clostridium perfringens has been reported as the cause of up to 15% of cases of antibiotic-associated diarrhoea (AAD) and may be diagnosed by detection of enterotoxin (CPEnt) in faeces. The performance of a commercial ELISA method for CPEnt, with culture and PCR methods to confirm the presence of enterotoxigenic C. pe...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-51-10-891

    authors: Asha NJ,Wilcox MH

    更新日期:2002-10-01 00:00:00

  • K1, K5 and O antigens of Escherichia coli in relation to serum killing via the classical and alternative complement pathways.

    abstract::The sensitivity of Escherichia coli strains to 80% normal human serum (NHS) and the relative importance of the classical and alternative complement pathways was assessed in relation to K1, K5, and O antigen carriage. Strains of each of the common O-serogroups, O1, O2, O4, O6, O7, O9, O18 and O75, smooth strains not ty...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-41-2-139

    authors: Devine DA,Roberts AP

    更新日期:1994-08-01 00:00:00

  • Effects of biliary diversion on intestinal microflora in the rat.

    abstract::Certain bile acids inhibit the growth in vitro of organisms commonly found in the intestine and, in particular, anaerobic bacteria. As the importance of this effect in vivo has not been demonstrated, we investigated changes in intestinal flora in the rat after diversion of bile to the urinary bladder. Five days after ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-10-2-241

    authors: Burke V,Stone DE,Beaman J,Gracey M

    更新日期:1977-05-01 00:00:00

  • A severe outbreak of listeriosis in central Italy with a rare pulsotype associated with processed pork products.

    abstract:PURPOSE:From May 2015 to March 2016, an outbreak due to Listeria monocytogenes serotype 1/2a and clinical pulsotype never previously isolated in Europe occurred in central Italy, involving 24 confirmed clinical cases. The article provides a description of the outbreak and the investigation carried out by a multidiscipl...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000785

    authors: Duranti A,Sabbatucci M,Blasi G,Acciari VA,Ancora M,Bella A,Busani L,Centorame P,Cammà C,Conti F,De Medici D,Di Domenico M,Di Marzio V,Filippini G,Fiore A,Fisichella S,Gattuso A,Gianfranceschi M,Graziani C,Guidi F,

    更新日期:2018-09-01 00:00:00

  • Inhibitory efficacy of geraniol on biofilm formation and development of adaptive resistance in Staphylococcus epidermidis RP62A.

    abstract:PURPOSE:The current study has been designed to delineate the efficacy of geraniol (GE) on biofilm formation in Staphylococcus epidermidis as well as the effect of subinhibitory concentrations of GE on the development of adaptive resistance. METHODOLOGY:Biofilm biomass quantification assay was performed to evaluate the...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000570

    authors: Kannappan A,Sivaranjani M,Srinivasan R,Rathna J,Pandian SK,Ravi AV

    更新日期:2017-10-01 00:00:00

  • Administration of a nitric oxide donor inhibits mglA expression by intracellular Francisella tularensis and counteracts phagosomal escape and subversion of TNF-α secretion.

    abstract::Francisella tularensis is a highly virulent intracellular bacterium capable of rapid multiplication in phagocytic cells. Previous studies have revealed that activation of F. tularensis-infected macrophages leads to control of infection and reactive nitrogen and oxygen species make important contributions to the bacter...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.032870-0

    authors: Tancred L,Telepnev MV,Golovliov I,Andersson B,Andersson H,Lindgren H,Sjöstedt A

    更新日期:2011-11-01 00:00:00

  • Candida albicans isolates from HIV-infected and AIDS patients exhibit enhanced adherence to epithelial cells.

    abstract::The increased prevalence of oral candidosis associated with HIV infection must be intrinsically related to immunological changes in the host, but might also involve alterations to the infecting strains of yeast. This study aimed to determine if strains of Candida albicans isolated from asymptomatic HIV-infected indivi...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-43-6-452

    authors: Sweet SP,Cookson S,Challacombe SJ

    更新日期:1995-12-01 00:00:00

  • Modelling persistent Mycoplasma pneumoniae biofilm infections in a submerged BEAS-2B bronchial epithelial tissue culture model.

    abstract::Introduction. Infections with the respiratory pathogen Mycoplasma pneumoniae are often chronic, recurrent and resistant, persisting after antibiotic treatment. M. pneumoniae grown on glass forms protective biofilms, consistent with a role for biofilms in persistence. These biofilms consist of towers of bacteria inters...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.001266

    authors: Feng M,Burgess AC,Cuellar RR,Schwab NR,Balish MF

    更新日期:2021-01-01 00:00:00

  • Successfully treated spondylodiscitis due to a previously unreported mycobacterium.

    abstract::A non-tuberculous mycobacterium was isolated, following a vertebral needle aspiration, from the blood of a patient with severe spondylodiscitis. The strain turned out to be different from any known mycobacterial species and was quite drug-susceptible in vitro. The patient improved markedly following treatment with mer...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.46256-0

    authors: Tortoli E,Mantella A,Mariottini A,Mazzarelli G,Pecile P,Rogasi PG,Sterrantino G,Fantoni E,Leoncini F

    更新日期:2006-01-01 00:00:00

  • Identification of catalase-like activity from Mycobacterium leprae and the relationship between catalase and isonicotinic acid hydrazide (INH).

    abstract::As Mycobacterium leprae proliferate inside macrophages, it has been speculated that catalase encoded by katG may protect the bacilli from deleterious effects of peroxide generated from the macrophage and may also play a crucial role in the survival of M. leprae in vivo. However, unlike that of M. tuberculosis, the kat...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-50-8-675

    authors: Kang TJ,You JC,Chae GT

    更新日期:2001-08-01 00:00:00

  • Identification of Helicobacter in gastric biopsies by PCR based on 16S rDNA sequences: a matter of little significance for the prediction of H. pylori-associated gastritis?

    abstract::The aim of the present study was to correlate molecular evidence of the presence of Helicobacter pylori in gastric biopsy samples, based on analysis of 16S rDNA, vacuolating toxin (vacA), urease A (ureA) and cagA genes, with the clinical, histological and serological findings in patients with H. pylori-associated gast...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-47-8-695

    authors: Tiveljung A,Borch K,Jonasson J,Mårdh S,Petersson F,Monstein HJ

    更新日期:1998-08-01 00:00:00

  • Shiga toxin-producing Escherichia coli isolated from patients with diarrhoea in Bangladesh.

    abstract::The prevalence of Shiga toxin-producing Escherichia coli (STEC) and its characteristics were determined among hospitalized patients with diarrhoea and children with diarrhoea in an urban slum community of Dhaka city using sensitive culture and PCR methods. Stool samples were collected from 410 patients with diarrhoea ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.46916-0

    authors: Islam MA,Heuvelink AE,de Boer E,Sturm PD,Beumer RR,Zwietering MH,Faruque ASG,Haque R,Sack DA,Talukder KA

    更新日期:2007-03-01 00:00:00

  • Ovine systemic pasteurellosis caused by Pasteurella haemolytica biotype T.

    abstract::A detailed study was made of lambs aged 5--7 months naturally infected with Pasteurella haemolytica biotype T. In addition to the well known features of such infections, previously unreported necrotic lesions of the tonsil, oesophagus, pharynx and adjacent areas were consistently seen. Large numbers of P. haemolytica ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-14-1-89

    authors: Dyson DA,Gilmour NJ,Angus KW

    更新日期:1981-02-01 00:00:00

  • Denaturing gradient gel electrophoresis analysis for the detection of point mutations in the Chlamydia trachomatis major outer-membrane protein gene.

    abstract::Fifty clinical strains of Chlamydia trachomatis were studied by denaturing gradient gel electrophoresis (DGGE) of bacterial DNA amplified by the polymerase chain reaction (PCR). The strains belonged to the three most commonly encountered serovars in developed countries--D, E and F. Six reference strains, including the...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-43-1-14

    authors: Sayada C,Denamur E,Grandchamp B,Orfila J,Elion J

    更新日期:1995-07-01 00:00:00

  • Distribution of aggA and aafA gene sequences among Escherichia coli isolates with genotypic or phenotypic characteristics, or both, of enteroaggregative E. coli.

    abstract::Two types of fimbriae, designated aggregative adherence fimbria I and II (AAF/I and AAF/II) have been described in enteroaggregative Escherichia coli (EAEC) strains. These fimbriae mediate the aggregative pattern of adherence (AA) to epithelial cells. The genes encoding the structural subunit of each fimbria have been...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-48-6-597

    authors: Elias WP,Suzart S,Trabulsi LR,Nataro JP,Gomes TAT

    更新日期:1999-06-01 00:00:00

  • Characterization of the clonal subpopulation Fiocruz L1-130 of Leptospira interrogans in rats and dogs from Brazil.

    abstract:PURPOSE:Leptospira interrogans serogroup Icterohaemorrhagiae strains have been described as causing disease in both humans and animals and as being present worldwide. Icterohaemorrhagiae and Copenhageni serovars are known to cause severe disease in their hosts, and zoonotic outbreaks have been described. The genetic si...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000806

    authors: Jaeger LH,Pestana CP,Carvalho-Costa FA,Medeiros MA,Lilenbaum W

    更新日期:2018-09-01 00:00:00

  • Comparison of four automated nucleic acid extraction platforms for the recovery of DNA from Aspergillus fumigatus.

    abstract::Invasive aspergillosis is a significant cause of morbidity and mortality within at-risk groups. Directed antifungal chemotherapy, guided by effective screening algorithms that incorporate reliable and validated molecular assays, reduces the morbidity associated with empirical administration and allows earlier diagnosi...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.076315-0

    authors: Perry MD,White PL,Barnes RA

    更新日期:2014-09-01 00:00:00

  • Potential of exocellular carbohydrate antigens of Staphylococcus epidermidis in the serodiagnosis of orthopaedic prosthetic infection.

    abstract::The potential of exocellular carbohydrate antigens of Staphylococcus epidermidis as markers of infection in bone was investigated by immunoblotting and enzyme-linked immunosorbent assay (ELISA). Exocellular antigens were prepared by gel filtration chromatography of concentrated brain heart infusion culture supernates....

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-44-5-355

    authors: Lambert PA,Van Maurik A,Parvatham S,Akhtar Z,Fraise AP,Krikler SJ

    更新日期:1996-05-01 00:00:00

  • Expression of prostaglandin receptors in Chlamydia trachomatis-infected recurrent spontaneous aborters.

    abstract::A study was undertaken to quantify the expression of prostaglandin (PG) receptors and find the effect of gestational age on expression of PG receptor genes in Chlamydia trachomatis-infected recurrent spontaneous aborters (RSA). Endometrial curettage tissue (ECT) was collected from 130 RSA (Group I) and 100 age-matched...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000256

    authors: Singh N,Prasad P,Singh LC,Das B,Rastogi S

    更新日期:2016-06-01 00:00:00

  • The value of LYM-1 cells for examining vacuole formation and loss of cell viability induced by culture supernates of Helicobacter pylori.

    abstract::Some strains of Helicobacter pylori are known to produce an extracellular cytotoxin that causes vacuolation in cultured mammalian cells. Screening for such strains makes use of HeLa cells which may not be sensitive enough to detect minimal changes. The aim of this study was to develop a more sensitive cell line. Vacuo...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-46-8-705

    authors: Wang XM,Kojima T,Satoh K,Taniguchi Y,Tokumaru K,Saifuku K,Seki M,Kihira K,Ido K,Uchida JY,Ohmori C,Takaoka T,Kimura K

    更新日期:1997-08-01 00:00:00