Abstract:
:The advent of next-generation sequencing technology has revolutionized the field of prokaryotic genetics and genomics by allowing interrogation of entire genomes, transcriptomes and global transcription factor binding profiles. As more studies employing these techniques have been performed, the state of the art regarding prokaryotic gene regulation has developed from the level of individual genes to genetic regulatory networks and systems biology. When applied to bacterial pathogens, particularly valuable insights have been gained into the regulation of virulence-associated genes, their relative importance to bacterial survival in planktonic, biofilm or host infection scenarios, antimicrobial resistance and the molecular details of host-pathogen interactions. This review outlines some of the latest developments and applications of next-generation sequencing techniques that have used primarily Pseudomonas aeruginosa as a model system. We focus particularly on insights into Pseudomonas virulence and infection that have been gained from these approaches and the future directions in which this field could develop.
journal_name
J Med Microbioljournal_title
Journal of medical microbiologyauthors
Valli RXE,Lyng M,Kirkpatrick CLdoi
10.1099/jmm.0.001135subject
Has Abstractpub_date
2020-02-01 00:00:00pages
162-175issue
2eissn
0022-2615issn
1473-5644journal_volume
69pub_type
杂志文章,评审abstract::Bifidobacteria are dominant in the gut of full-term infants, although colonisation by them is often delayed in preterm neonates. Bifidobacteria are recognised to have beneficial effects on digestive disorders and they might prevent neonatal necrotising enterocolitis (NEC), a gastrointestinal disease that predominantly...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-48-1-89
更新日期:1999-01-01 00:00:00
abstract::Faeces from urban children < 2 years old with acute diarrhoeal illness and from non-diarrhoeal infants (controls) were examined for Escherichia coli and other enteropathogens. A total of 990 E. coli isolates from 100 patients and 50 controls was tested for enteropathogenic E. coli (EPEC) serotype (O:H), adherence to H...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-47-9-781
更新日期:1998-09-01 00:00:00
abstract::The aim of this study was to evaluate the toxigenic status of circulating strains of Clostridium difficile in a large teaching hospital. Overall 220 isolates were studied of which 199 (90.5 %) produced both large clostridial toxins detected by conventional methods. Ten more strains (4.5 %) had toxin A and B genes dete...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.45809-0
更新日期:2005-02-01 00:00:00
abstract::Members of the genus Actinomyces, Gram-positive, non-spore-forming anaerobic bacteria, are normal inhabitants of the mucosal surfaces of the oral, gastrointestinal and genital tracts. Identification of these bacteria using conventional methods is generally difficult because of their complex transport and growth requir...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.053165-0
更新日期:2013-05-01 00:00:00
abstract::Infection of humans by Abiotrophia defectiva, a nutritionally variant streptococcus, most commonly takes the form of endocarditis, though a variety of other manifestations ranging from central nervous system abscesses to orthopaedic infections have been seen. We report here what we believe is the first case of bullous...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.041301-0
更新日期:2012-07-01 00:00:00
abstract::Shiga toxins (Stx1 and Stx2) are responsible for initiating haemolytic uraemic syndrome, a serious extraintestinal complication caused by enterohaemorrhagic Escherichia coli O157 : H7 infection in humans. Shiga toxins are classical AB(5)-type exotoxins, consisting of a globotriaosylceramide (Gb(3))-binding B subunit p...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.47282-0
更新日期:2007-11-01 00:00:00
abstract::Isolates of Vibrio cholerae O1 biotype El Tor serotype Inaba associated with an outbreak of cholera in Trivandrum, southern India, were characterized. PCR testing revealed that all five isolates examined carried the TCP pathogenicity island, the CTX genetic element and the RTX toxin, and produced cholera toxin (CT). R...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.46868-0
更新日期:2007-02-01 00:00:00
abstract::Aspiration of oropharyngeal bacteria and fungi is occasionally suspected in patients with pneumonia. A patient with oral carcinoma underwent chemoradioimmunotherapy and, about 4 weeks from the start of the therapy, the patient suffered from severe oral mucositis induced by chemoradiotherapy, and candidal pneumonia was...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.45769-0
更新日期:2005-05-01 00:00:00
abstract::A serial multiplex PCR approach was reformulated for pneumococcal serotyping to test 153 clinical isolates from children in Mozambique. This approach identified serotypes in 139 (90.8%) of 153 isolates; 126 (82.4%) were identified within two reactions. This approach in developing countries would require minimal traini...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.47346-0
更新日期:2007-09-01 00:00:00
abstract::The bactericidal activity of compound DR-3355, an optically active isomer of ofloxacin, was measured against Escherichia coli, Staphylococcus aureus and S. epidermidis, in nutrient broth and in phosphate-buffered saline. DR-3355 was found to be approximately twice as active as ofloxacin in terms of the concentration a...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-30-3-227
更新日期:1989-11-01 00:00:00
abstract::The bactericidal action of non-immune whole blood on Proteus mirabilis was increased when the bacteria contained the R-plasmid RP1. This effect was due mainly to increased phagocytosis. Iron-depleted stationary-phase cells were more sensitive than carbon-depleted cells. The contribution of serum was usually negligible...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-24-3-233
更新日期:1987-11-01 00:00:00
abstract::A series of 178 strains of Escherichia coli, highly resistant to trimethoprim, isolated from hospital patients and patients in the community between 1979 and 1983, was examined for the presence of Tn7 on a plasmid or on the chromosome only, by transposition to RP4 and restriction endonuclease digestion with Hind III. ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-22-2-125
更新日期:1986-09-01 00:00:00
abstract::The aim of this study was to establish bacterial profiles in gastric biopsy specimens from patients with Helicobacter pylori-associated gastritis by means of temporal temperature gradient gel electrophoresis (TTGE) of PCR-amplified 16S rDNA fragments. Specimens from eight patients with asymptomatic gastritis and five ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/0022-1317-49-9-817
更新日期:2000-09-01 00:00:00
abstract::The opsonic and protective capacities of rabbit antisera against Escherichia coli O, K and core-glycolipid cell-wall antigens were compared with specific antibody titres as measured by agglutination and enzyme-linked immunosorbent assay. Anti-O antisera were opsonic and protective against two noncapsulate strains. Onl...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-14-4-381
更新日期:1981-11-01 00:00:00
abstract::The production of toxins A and B by Clostridium difficile was greatly enhanced under biotin-limited conditions, in which a 140-kDa protein was expressed strongly. Gene cloning revealed that this protein was a homologue of formylglycinamidine ribonucleotide synthetase (FGAM synthetase, EC 6.3.5.3), which is known as Pu...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/0022-1317-51-1-34
更新日期:2002-01-01 00:00:00
abstract::The antifungal susceptibilities of 598 isolates of Candida spp. (bloodstream and other sterile sites) to liposomal amphotericin B (L-AmB) versus amphotericin B (AmB) were determined. MICs were calculated using the Clinical and Laboratory Standards Institute broth microdilution (M27-A3) method for L-AmB and the Etest m...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000402
更新日期:2017-03-01 00:00:00
abstract::A novel bunyavirus associated with fever, thrombocytopenia and leukopenia syndrome (FTLS) was discovered in Henan Province, China. Here, we report the development of an assay for this novel bunyavirus based on real-time reverse transcription PCR (RT-PCR). The assay exhibited high sensitivity and specificity without cr...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.049577-0
更新日期:2013-07-01 00:00:00
abstract::Chlamydia trachomatis is one of the most common sexually transmitted pathogens in the world and often causes chronic inflammatory diseases that are insensitive to antibiotics. The type 3 secretion system (T3SS) of pathogenic bacteria is a promising target for therapeutic intervention aimed at bacterial virulence and c...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000189
更新日期:2016-01-01 00:00:00
abstract::In January 2015, Public Health England and the United Kingdom (UK) Ministry of Defence investigated cases of diarrhoea and fever in military personnel recently returned to the UK after supporting the response to the Ebola epidemic in Sierra Leone. Tests for Ebola virus infection were negative. PCR tests detected the i...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000832
更新日期:2018-11-01 00:00:00
abstract::Stenotrophomonas maltophilia is an opportunist multidrug-resistant pathogen that causes a wide range of nosocomial infections. Various cystic fibrosis (CF) centres have reported an increasing prevalence of S. maltophilia colonization/infection among patients with this disease. The purpose of this study was to assess s...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.076950-0
更新日期:2014-12-01 00:00:00
abstract::Data on the occurrence and epidemiology of Aspergillus spp. in laying hens farms are scant. With the aims of determining levels of airborne contamination in laying hen farms and evaluating the potential risk of infection for workers and animals, 57 air samples from 19 sheds (Group I), 69 from faeces (Group II), 19 fro...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.065946-0
更新日期:2014-03-01 00:00:00
abstract::Four hundred and twenty-seven Escherichia coli isolates from 427 cases of infantile diarrhoea in Poland, belonging to serogroups O18, O26, O44, O86, O126 and O127 and 150 E. coli isolates from 52 healthy children were examined for selected virulence properties. The presence of the plasmid pAA, a plasmid encoding enter...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/0022-1317-51-7-548
更新日期:2002-07-01 00:00:00
abstract::The cystic fibrosis (CF) airways are colonized by polymicrobial communities with high bacterial load and are influenced by frequent antibiotic exposures. This community includes diverse streptococci, some of which have been directly or indirectly associated with pulmonary exacerbations. As many streptococci are natura...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000172
更新日期:2015-11-01 00:00:00
abstract::Preliminary differentiation of Leptospira interrogans serovars hardjo and balcanica can be made with antisera that are collected from hamsters after experimental infection with serovar hardjo and selected for high homologous and low or nil heterologous titres, as measured by the microscopic agglutination test. Rabbit ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-11-3-351
更新日期:1978-08-01 00:00:00
abstract::Rapid diagnosis of multidrug-resistant tuberculosis (MDR-TB) is essential for the prompt initiation of effective second-line therapy to improve treatment outcome and limit transmission of this obstinate disease. A variety of molecular methods that enable the rapid detection of mutations implicated in MDR-TB have been ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.032292-0
更新日期:2011-10-01 00:00:00
abstract::The substrates inositol, rhamnose, d-tartrate and m-tartrate used in fermentation tests with 338 cultures of Salmonella paratyphi B differentiated strains in some phage types to give information that could be used in epidemiological investigations. Xylose in Bitter's medium, the fifth substrate by which 13 of a potent...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-26-4-285
更新日期:1988-08-01 00:00:00
abstract:PURPOSE:Persister cells, a subpopulation of tolerant cells within the bacterial culture, are commonly thought to be responsible for antibiotic therapy failure and infection recurrence. Klebsiella pneumoniae is a notorious human pathogen for its increasing resistance to antibiotics and wide involvement in severe infecti...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000680
更新日期:2018-03-01 00:00:00
abstract::Staphylococcus aureus is a leading cause of hospital-acquired (HA) and community-acquired (CA) infections worldwide. Recently, S. aureus strains resistant to meticillin (MRSA) have become established within both communities. We isolated 314 isolates of MRSA from hospitalized patients in a referral hospital (HA isolate...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.070722-0
更新日期:2014-06-01 00:00:00
abstract::The abilities of six strains of Salmonella typhimurium to associate with rabbit ileal mucosa have been measured in vitro. Two were "virulent" strains (TML and W118 which are invasive and inducers of fluid secretion in rabbit ileal loops); four were "avirulent" (LT7, M206 and SL1027 which are invasive but induce neglig...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-29-4-283
更新日期:1989-08-01 00:00:00
abstract::The effect of CO2 on the growth of 31 strains of Candida albicans was studied in serum and in a defined medium containing urea, ammonium chloride, asparagine, glutamine or acetamide as the nitrogen source. CO2 10% enhanced the mycelial growth of all strains when the medium contained an appropriate constituent to media...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-22-3-203
更新日期:1986-11-01 00:00:00