Profiling of bacterial flora in gastric biopsies from patients with Helicobacter pylori-associated gastritis and histologically normal control individuals by temperature gradient gel electrophoresis and 16S rDNA sequence analysis.

abstract：:The distribution of cryptic plasmids among 123 isolates of Providencia stuarti from a hospital ward during a prospective epidemiological study is reported. Two closely related stable plasmids (34 Kb and 36 Kb) were identified by restriction endonuclease digest analysis of plasmid DNA. One or other of these cryptic pla...

journal_title：Journal of medical microbiology

authors： Hawkey PM,Bennett PM,Hawkey CA

更新日期：1984-10-01 00:00:00

abstract：PURPOSE:We previously identified an association between CC22 meticillin-resistant Staphylococcus aureus (MRSA) bloodstream infection isolates with an elevated vancomycin MIC (V-MIC) in the susceptible range (1.5-2 mg l-1) and endocarditis. This study explores whether these isolates have a specific phenotype consistent ...

journal_title：Journal of medical microbiology

authors： Marbach H,Boakes E,Lynham S,Ward M,Otter JA,Edgeworth JD

更新日期：2017-05-01 00:00:00

abstract：:Eight monoclonal antibodies (MAbs) were produced from mice immunised with whole cells of heat-killed Pasteurella multocida type A which had been cultured under iron-restricted conditions. The MAbs were selected by an enzyme-linked immunosorbent assay (ELISA) in which the antigen consisted of whole bacteria of the immu...

journal_title：Journal of medical microbiology

authors： Wijewardana TG,Wilson CF,Gilmour NJ,Poxton IR

更新日期：1990-12-01 00:00:00

abstract：PURPOSE:Diabetic patients seem to be predisposed to cutaneous candidiasis. In this study, we evaluated the interference of diabetic conditions in alloxan-induced diabetic mice in relation to the development of C. albicans infection, density of M1 and M2 macrophages, distribution of collagen type I and III and anti-infl...

journal_title：Journal of medical microbiology

authors： Pupim ACE,Campois TG,Araújo EJA,Svidizinski TIE,Felipe I

更新日期：2017-06-01 00:00:00

abstract：:Using a Clostridium difficile glutamate dehydrogenase (GDH) immunoassay and a sensitive C. difficile toxin A/B immunoassay, human stool specimens from patients with diarrhoea (n = 1085) were classified as either GDH positive/toxin negative, or GDH positive/toxin positive. Overall, 528/725 (73%) of the GDH-positive/tox...

journal_title：Journal of medical microbiology

authors： Fairley DJ,McKenna JP,Stevenson M,Weaver J,Gilliland C,Watt A,Coyle PV

更新日期：2015-11-01 00:00:00

abstract：:In Mexico, there is a lack of up-to-date published data that show viruses to be the main cause of acute respiratory infection (ARI). The objective of this study was to estimate the comparative viral frequency between children under the age of 5 years with and without ARI (n = 179 in each group) in a suburban community...

journal_title：Journal of medical microbiology

authors： Manjarrez ME,Rosete DP,Rincón M,Villalba J,Cravioto A,Cabrera R

更新日期：2003-07-01 00:00:00

abstract：:Limited digestion of staphylococcal enterotoxin B (SEB) with trypsin resulted in the generation of a 12-Kda amino-terminal fragment and a 17-Kda carboxy-terminal fragment which were isolated by preparative iso-electric focusing. The carboxy-terminal fragment exhibited significant mitogenic activity for murine splenocy...

journal_title：Journal of medical microbiology

authors： Binek M,Newcomb JR,Rogers CM,Rogers TJ

更新日期：1992-03-01 00:00:00

abstract：:Prevotella intermedia and the newly described P. nigrescens cannot be reliably distinguished by phenotypic tests. In this study, restriction endonuclease digestion of amplified 16S rDNA (16S rDNA PCR-RFLP) was used to generate restriction profiles of the type strains of P. intermedia and P. nigrescens and 43 fresh iso...

journal_title：Journal of medical microbiology

authors： Milsom SE,Sprague SV,Dymock D,Weightman AJ,Wade WG

更新日期：1996-01-01 00:00:00

abstract：:Five hundred clinical group A streptococcal (GAS) isolates were collected in Belgium during the period 1 Nov. 1993 to 31 Oct. 1994. Clinical and laboratory data were recorded and isolates were characterised. The presence of the genes encoding streptococcal pyrogenic exotoxin types A (speA), B (speB), C (speC), F (speF...

journal_title：Journal of medical microbiology

authors： Descheemaeker P,VAN Loock F,Hauchecorne M,Vandamme P,Goossens H

更新日期：2000-05-01 00:00:00

abstract：:Oral malodour is thought to be caused mainly by the production of volatile sulfide compounds (VSCs) by anaerobic Gram-negative oral bacteria. Previous studies have shown that these bacteria are susceptible to blue light (400-500 nm wavelength). In the present study, we tested the effect of blue light in the presence o...

journal_title：Journal of medical microbiology

authors： Sterer N,Jeffet U,Dadoun A,Greenstein RB,Kohavi D

更新日期：2014-08-01 00:00:00

abstract：:Bacteraemia in children is a potentially life-threatening condition. The objective of this study was to determine trends in the aetiology of bacteraemia in children aged 1 month-15 years in England and Wales by collecting data voluntarily reported by National Health Service hospital microbiology laboratories. Over the...

journal_title：Journal of medical microbiology

authors： Henderson KL,Johnson AP,Muller-Pebody B,Charlett A,Gilbert R,Sharland M

更新日期：2010-02-01 00:00:00

abstract：:Helicobacter pylori is believed to trigger neutrophil activation through several factors, including the H. pylori neutrophil-activating protein (HpNAP). The aim of this study was to characterise the factors within H. pylori cell-free extracts that stimulate neutrophil activation. Neutrophil activation was found to be ...

journal_title：Journal of medical microbiology

authors： Leakey A,Hirst R,LA Brooy J

更新日期：2001-09-01 00:00:00

abstract：:Members of the Streptococcus anginosus group (SAG) are frequently involved in pyogenic infections in humans. In the present study, the antimicrobial susceptibility of 141 clinical SAG isolates to six antimicrobial agents was analysed by agar dilution. All isolates were susceptible to penicillin, cefotaxime and vancomy...

journal_title：Journal of medical microbiology

authors： Asmah N,Eberspächer B,Regnath T,Arvand M

更新日期：2009-02-01 00:00:00

abstract：:Aminoglycoside-resistant variants of Pseudomonas aeruginosa strain PAO1 were readily selected by culturing the organism in medium containing increasing concentrations of gentamicin, tobramycin or amikacin until the strains were growing in a concentration of drug 128-fold greater than the minimal inhibitory concentrati...

journal_title：Journal of medical microbiology

authors： Gilleland LB,Gilleland HE,Gibson JA,Champlin FR

更新日期：1989-05-01 00:00:00

abstract：:Breakthrough contamination of tuberculosis (TB) cultures is a problem in that it allows the overgrowth of another bacterium present in the sputum specimen, which can potentially mask the presence of Mycobacterium tuberculosis. The aim of this study was to isolate and characterize the bacterial organisms responsible fo...

journal_title：Journal of medical microbiology

authors： McClean M,Stanley T,Stanley S,Maeda Y,Goldsmith CE,Shepherd R,Millar BC,Dooley JS,Moore JE

更新日期：2011-09-01 00:00:00

abstract：:In this study, the species diversity of staphylococci with inducible resistance to macrolides, lincosamides and streptogramin B (MLSB) isolated from clinical samples, sewage and river water was investigated. Inducible clindamycin resistance was tested using a D-test and macrodilution assays. Inducible cross-resistance...

journal_title：Journal of medical microbiology

authors： Heß S,Gallert C

更新日期：2014-11-01 00:00:00

abstract：:Stenotrophomonas maltophilia is an opportunist multidrug-resistant pathogen that causes a wide range of nosocomial infections. Various cystic fibrosis (CF) centres have reported an increasing prevalence of S. maltophilia colonization/infection among patients with this disease. The purpose of this study was to assess s...

journal_title：Journal of medical microbiology

authors： Vidigal PG,Mosel F,Koehling HL,Mueller KD,Buer J,Rath PM,Steinmann J

更新日期：2014-12-01 00:00:00

abstract：:Two carbapenem-non-susceptible Klebsiella pneumoniae isolates, Z2554 and Z2110, were collected from a hospital in China and analysed by PFGE. K. pneumoniae Z2554 and Z2110 were genetically unrelated and showed resistance to ertapenem, and reduced susceptibility to imipenem and meropenem. Analysis of their beta-lactama...

journal_title：Journal of medical microbiology

authors： Wang XD,Cai JC,Zhou HW,Zhang R,Chen GX

更新日期：2009-09-01 00:00:00

abstract：:Acinetobacter baumannii is an opportunist nosocomial pathogen of world-wide importance and produces severe infections in immunocompromised patients. However, the virulence factors contributing to its pathogenic properties are not well known. The effect of normal human serum against 18 clinical isolates of the most pre...

journal_title：Journal of medical microbiology

authors： García A,Solar H,González C,Zemelman R

更新日期：2000-11-01 00:00:00

abstract：:The distribution of K1, K5 and O antigens was examined in 500 clinical strains of Escherichia coli. Of 400 strains from urine, 52% belonged to serogroups O1, O2, O4, O6, O7, O8, O9, O18, O25 and O75; 34% were non-typable (NT) and 14% were autoagglutinable (AA). Antigen K1 was carried by 17% of these strains, and K5 by...

journal_title：Journal of medical microbiology

authors： Devine DA,Robinson L,Roberts AP

更新日期：1989-12-01 00:00:00

abstract：:Introduction. Rapid identification of the causative agent of sepsis is crucial for patient outcomes.Aim. The Sepsityper sample preparation method enables direct microbial identification of positive blood culture samples via matrix-assisted laser desorption ionization/time-of-flight mass spectrometry (MALDI-TOF MS).Hyp...

journal_title：Journal of medical microbiology

authors： Cordovana M,Zignoli A,Ambretti S

更新日期：2020-12-01 00:00:00

abstract：:Helicobacter pylori infection represents a key factor in the aetiology of various gastrointestinal diseases. H. pylori infection diagnosis is generally achieved using both invasive (e.g. biopsy of the gastric epithelium) and non-invasive methods. Therefore, cultivation on a growth medium becomes complex. Trypsin is a ...

journal_title：Journal of medical microbiology

authors： Peretz A,Paritsky M,Pastukh N,Koifman A,Brodsky D,Glyatman T,On A

更新日期：2015-06-01 00:00:00

abstract：:A non-tuberculous mycobacterium was isolated, following a vertebral needle aspiration, from the blood of a patient with severe spondylodiscitis. The strain turned out to be different from any known mycobacterial species and was quite drug-susceptible in vitro. The patient improved markedly following treatment with mer...

journal_title：Journal of medical microbiology

authors： Tortoli E,Mantella A,Mariottini A,Mazzarelli G,Pecile P,Rogasi PG,Sterrantino G,Fantoni E,Leoncini F

更新日期：2006-01-01 00:00:00

abstract：:The purpose of this study was to verify whether the presence of any of the Helicobacter pylori cagPAI genes or segments--cagA, cagA promoter, cagE, cagM, tnpB, tnpA, cagT and the left end of the cag II (LEC) region--would be a useful marker for the risk of peptic ulcer disease development. H. pylori DNA extracted from...

journal_title：Journal of medical microbiology

authors： Mattar R,Marques SB,Monteiro MDS,Dos Santos AF,Iriya K,Carrilho FJ

更新日期：2007-01-01 00:00:00

abstract：:The ability of rabbits to clear an intraperitoneal injection of Pseudomonas aeruginosa in the presence or absence of a surgically implanted peritoneal device was investigated. Sham-operated rabbits without an implant eliminated a P. aeruginosa challenge of 5 x 10(6) cfu/ml; lavage fluid and peritoneal tissues became c...

journal_title：Journal of medical microbiology

authors： Ward KH,Olson ME,Lam K,Costerton JW

更新日期：1992-06-01 00:00:00

abstract：:The effects of ethyleneglycoltetra-acetic acid (EGTA) and EGTA + magnesium (MgEGTA) on the viable counts of 10 strains of Escherichia coli O6 have been studied in normal human serum (NHS), heat-inactivated serum (HIS) and in culture media with and without the addition of a beta-lactam antibiotic. The addition of EGTA ...

journal_title：Journal of medical microbiology

authors： Roberts AP,Phillips R

更新日期：1981-05-01 00:00:00

abstract：PURPOSE:Infections associated with Acinetobacter baumannii represent an increasing threat in healthcare settings. Therefore, we investigated the epidemiological relationship between clinical isolates of A. baumannii obtained from patients in a university hospital in Bandar Abbas in southern Iran. METHODOLOGY:Sixty-fou...

journal_title：Journal of medical microbiology

authors： Saffari F,Monsen T,Karmostaji A,Azimabad FB,Widerström M

更新日期：2017-11-01 00:00:00

abstract：:Amplification and sequence analysis of the 16S rRNA gene can be applied to detect and identify bacteria in clinical samples. We examined 75 clinical samples (17 culture-positive, 58 culture-negative) prospectively by two different PCR protocols, amplifying either a single fragment (1343 bp) or two fragments (762/598 b...

journal_title：Journal of medical microbiology

authors： Jenkins C,Ling CL,Ciesielczuk HL,Lockwood J,Hopkins S,McHugh TD,Gillespie SH,Kibbler CC

更新日期：2012-04-01 00:00:00

abstract：:To obtain monoclonal antibodies (MAbs) directed preferentially against the pathogenic phase of Candida albicans, mice were immunised with germ tubes of C. albicans serotype A, strain VW.32, killed by exposure to ultraviolet (UV) irradiation. Fusions were performed either by the standard chemical procedure with polyeth...

journal_title：Journal of medical microbiology

authors： Fortier B,Hopwood V,Poulain D

更新日期：1988-12-01 00:00:00

abstract：:Leishmaniasis consists of a complex spectrum of infectious diseases with worldwide distribution of which visceral leishmaniasis or kala-azar caused by Leishmania donovani is the most devastating. In the absence of vaccines, chemotherapy remains the mainstay for the control of leishmaniasis. The drugs of choice are exp...

journal_title：Journal of medical microbiology

authors： Islamuddin M,Sahal D,Afrin F

更新日期：2014-01-01 00:00:00