Differential binding of Shiga toxin 2 to human and murine neutrophils.

Abstract:

:Shiga toxins (Stx1 and Stx2) are responsible for initiating haemolytic uraemic syndrome, a serious extraintestinal complication caused by enterohaemorrhagic Escherichia coli O157 : H7 infection in humans. Shiga toxins are classical AB(5)-type exotoxins, consisting of a globotriaosylceramide (Gb(3))-binding B subunit pentamer and an enzymic A subunit. It is demonstrated in this study that Stx2 binds to human neutrophils by a non-classical mechanism that is independent of Gb(3). In contrast, the investigation revealed that Stx2 binds to murine neutrophils by the classical Gb(3)-dependent mechanism. Moreover, whereas the human serum amyloid P (HuSAP) component inhibited Stx2 binding to murine neutrophils, HuSAP increased Stx2 binding to human neutrophils by 84.2 % (P< or =0.002, Student's t-test). These observations may explain why HuSAP protects mice from the lethal effects of Stx2, whereas there is no indication that HuSAP plays a similar protective role in humans infected by E. coli O157 : H7.

journal_name

J Med Microbiol

authors

Griener TP,Mulvey GL,Marcato P,Armstrong GD

doi

10.1099/jmm.0.47282-0

subject

Has Abstract

pub_date

2007-11-01 00:00:00

pages

1423-1430

issue

Pt 11

eissn

0022-2615

issn

1473-5644

journal_volume

56

pub_type

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