Clonality, virulence and antimicrobial resistance of enteroaggregative Escherichia coli from Mirzapur, Bangladesh.

Abstract:

PURPOSE:This study investigates the virulence and antimicrobial resistance in association with common clonal complexes (CCs) of enteroaggregative Escherichia coli (EAEC) isolated from Bangladesh. The aim was to determine whether specific CCs were more likely to be associated with putative virulence genes and/or antimicrobial resistance. METHODOLOGY:The presence of 15 virulence genes (by PCR) and susceptibility to 18 antibiotics were determined for 151 EAEC isolated from cases and controls during an intestinal infectious disease study carried out between 2007-2011 in the rural setting of Mirzapur, Bangladesh (Kotloff KL, Blackwelder WC, Nasrin D, Nataro JP, Farag TH et al.Clin Infect Dis 2012;55:S232-S245). These data were then analysed in the context of previously determined serotypes and clonal complexes defined by multi-locus sequence typing. RESULTS:Overall there was no association between the presence of virulence or antimicrobial resistance genes in isolates of EAEC from cases versus controls. However, when stratified by clonal complex (CC) one CC associated with cases harboured more virulence factors (CC40) and one CC harboured more resistance genes (CC38) than the average. There was no direct link between the virulence gene content and antibiotic resistance. Strains within a single CC had variable virulence and resistance gene content indicating independent and multiple gene acquisitions over time. CONCLUSION:In Bangladesh, there are multiple clonal complexes of EAEC harbouring a variety of virulence and resistance genes. The emergence of two of the most successful clones appeared to be linked to either increased virulence (CC40) or antimicrobial resistance (CC38), but increased resistance and virulence were not found in the same clonal complexes.

journal_name

J Med Microbiol

authors

Chattaway MA,Day M,Mtwale J,White E,Rogers J,Day M,Powell D,Ahmad M,Harris R,Talukder KA,Wain J,Jenkins C,Cravioto A

doi

10.1099/jmm.0.000594

subject

Has Abstract

pub_date

2017-10-01 00:00:00

pages

1429-1435

issue

10

eissn

0022-2615

issn

1473-5644

journal_volume

66

pub_type

杂志文章
  • Nucleolar accumulation of Semliki Forest virus nucleocapsid C protein: influence of metabolic status, cytoskeleton and receptors.

    abstract::The nucleolar accumulation of Semliki Forest Virus (SFV) C protein was examined as a function of intact microtubules, intact microfilaments and accessible intermediate filaments. The cytoskeletal components do not seem to play a role in directing C protein to the nucleolus but nucleolar accumulation is energy-dependen...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-40-6-389

    authors: Jakob R

    更新日期:1994-06-01 00:00:00

  • Detection of clonal KPC-2-producing Klebsiella pneumoniae ST258 in Korea during nationwide surveillance in 2011.

    abstract::This study analysed the characteristics and genetic similarity of recent Klebsiella pneumoniae carbapenemase (KPC-2)-producing Klebsiella pneumoniae isolates from Korea. Recent laboratory surveillance detected an increase in carbapenemase-producing Enterobacteriaceae in Korea. A total of 6 KPC-2-producing K. pneumonia...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.059428-0

    authors: Yoo JS,Kim HM,Yoo JI,Yang JW,Kim HS,Chung GT,Lee YS

    更新日期:2013-09-01 00:00:00

  • Virulence properties of Escherichia coli faecal strains isolated in Poland from healthy children and strains belonging to serogroups O18, O26, O44, O86, O126 and O127 isolated from children with diarrhoea.

    abstract::Four hundred and twenty-seven Escherichia coli isolates from 427 cases of infantile diarrhoea in Poland, belonging to serogroups O18, O26, O44, O86, O126 and O127 and 150 E. coli isolates from 52 healthy children were examined for selected virulence properties. The presence of the plasmid pAA, a plasmid encoding enter...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-51-7-548

    authors: Paciorek J

    更新日期:2002-07-01 00:00:00

  • Incidence of common pyocin types of Pseudomonas aeruginosa from patients with cystic fibrosis and chronic airways diseases.

    abstract::We sought evidence to determine if particular strains of Pseudomonas aeruginosa have a predilection for pulmonary colonisation in patients with cystic fibrosis (CF). The incidence of common pyocin types in non-CF isolates (74%) was similar to that noted in previous reports but differed significantly (X2 = 16.7, p less...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-32-3-169

    authors: Tredgett MW,Doherty C,Govan JR

    更新日期:1990-07-01 00:00:00

  • The use of bacteriophages to differentiate serologically cross-reactive isolates of Klebsiella pneumoniae.

    abstract::In serological typing of Klebsiella pneumoniae strains from human, equine and environmental sources, the capsular identity of many isolates could not be determined because of serological cross-reactivity. A panel of 91 bacteriophages able to lyse each of the 77 capsular serotypes of K. pneumoniae was isolated and test...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-41-6-423

    authors: Pieroni P,Rennie RP,Ziola B,Deneer HG

    更新日期:1994-12-01 00:00:00

  • Assessment of possible tuberculous lymphadenopathy by PCR compared to non-molecular methods.

    abstract::Tuberculosis is a major public health problem in India and other developing countries and has formed a lethal partnership with AIDS. It often presents a diagnostic challenge especially when clinical presentation is suggestive but bacteriological proof is lacking. The objective of this study was to compare the various ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.45904-0

    authors: Pahwa R,Hedau S,Jain S,Jain N,Arora VM,Kumar N,Das BC

    更新日期:2005-09-01 00:00:00

  • Analysis of the porphyrin content of fluorescent pus by absorption spectrophotometry and high performance liquid chromatography.

    abstract::Extracts of 19 samples of pus which showed red fluorescence with ultraviolet light were screened for the presence of porphyrins by absorption spectrophotometry. All those which showed spectra typical of metal-free porphyrins were analysed by high performance liquid chromatography to identify the porphyrins present. Th...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-33-1-29

    authors: Brazier JS

    更新日期:1990-09-01 00:00:00

  • Candida krusei: biology, epidemiology, pathogenicity and clinical manifestations of an emerging pathogen.

    abstract::Early reports of Candida krusei in man describe the organism as a transient, infrequent isolate of minor clinical significance inhabiting the mucosal surfaces. More recently it has emerged as a notable pathogen with a spectrum of clinical manifestations such as fungaemia, endophthalmitis, arthritis and endocarditis, m...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章,评审

    doi:10.1099/00222615-41-5-295

    authors: Samaranayake YH,Samaranayake LP

    更新日期:1994-11-01 00:00:00

  • In-vitro characterisation of the phagocytosis and fate of anthrax spores in macrophages and the effects of anti-PA antibody.

    abstract::Antibodies (Abs) to the protective antigen (PA) component of the anthrax toxins have anti-spore as well as anti-toxin activities. Anti-PA antisera and purified anti-PA Abs enhance the phagocytosis by murine-derived macrophages (MQs) of spores of the Ames and Sterne strains and retard the germination of extracellular s...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-51-10-821

    authors: Welkos S,Friedlander A,Weeks S,Little S,Mendelson I

    更新日期:2002-10-01 00:00:00

  • Adhesion of clinical and environmental Aeromonas isolates to HEp-2 cells.

    abstract::A total of 63 Aeromonas strains isolated from diarrhoeal faeces or water samples were tested for adhesion to HEp-2 cells. An association between diarrhoea and high level adhesion was observed in that 12 of the 34 faecal isolates and none of the 29 environmental isolates yielded greater than 20 bacteria per HEp-2 cell ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-26-1-19

    authors: Carrello A,Silburn KA,Budden JR,Chang BJ

    更新日期:1988-05-01 00:00:00

  • Characterisation of cell-wall-derived polypeptide antigens from different species of Mycobacterium.

    abstract::Cell walls from different species of Mycobacterium were purified on a sucrose step gradient. The components derived from these preparations were characterised by sodium dodecyl sulphate-polyacrylamide gel electrophoresis, followed by staining or by Western blotting. Surface-exposed polypeptide molecules were also iden...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-32-4-263

    authors: Babu S,Jain NK,Prasad G,Bhattacharya S,Bhattacharya A

    更新日期:1990-08-01 00:00:00

  • Gene-expression profiles in gastric epithelial cells stimulated with spiral and coccoid Helicobacter pylori.

    abstract::Human gastric epithelial immortalized GES-1 cells were infected with spiral and coccoid Helicobacter pylori. Scanning electron microscopy was used to determine the ability of the two forms of H. pylori to adhere to GES-1 cells. GES-1 cell apoptosis induced by coccoid and spiral H. pylori was analysed using flow cytome...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.46456-0

    authors: Liu ZF,Chen CY,Tang W,Zhang JY,Gong YQ,Jia JH

    更新日期:2006-08-01 00:00:00

  • Electron microscopy studies on Gardnerella vaginalis grown in conventional and biofilm systems.

    abstract::The cell-wall characteristics of Gardnerella vaginalis grown in conventional and biofilm systems were studied by electron microscopy. The gram-positive nature of the cell wall was confirmed. Novel cell-wall particles which appeared to be associated with cell division were also identified, particularly in organisms of ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-48-2-211

    authors: Muli FW,Struthers JK,Tarpey PA

    更新日期:1999-02-01 00:00:00

  • Age-related changes in the prevalence of precipitating antibodies to BK virus in infants and children.

    abstract::Nearly 1000 sera from children were tested by immunoelectro-osmophoresis against BK virus, and age-specific prevalence rates were estimated from birth until the age of 12 years. Declining rates during the first 12 months showed the waning of passive immunity, which at birth reflects the mother's immune status. The cha...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-15-3-285

    authors: Dei R,Marmo F,Corte D,Sampietro MG,Franceschini E,Urbano P

    更新日期:1982-08-01 00:00:00

  • Isolation and characterisation of bacteria from abscesses in the subcutis of cats.

    abstract::Thirty-six closed abcesses in the subcutis of cats were examined. Of 168 bacterial strains isolated, 121(72%) were anaerobes and 47 (28%) were facultative anaerobes. Twenty-six abscesses contained mixtures of facultative anaerobes and anaerobes, six contained anaerobes only and four contained facultative anaerobes onl...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-12-2-207

    authors: Love DN,Jones RF,Bailey M,Johnson RS

    更新日期:1979-05-01 00:00:00

  • A severe outbreak of listeriosis in central Italy with a rare pulsotype associated with processed pork products.

    abstract:PURPOSE:From May 2015 to March 2016, an outbreak due to Listeria monocytogenes serotype 1/2a and clinical pulsotype never previously isolated in Europe occurred in central Italy, involving 24 confirmed clinical cases. The article provides a description of the outbreak and the investigation carried out by a multidiscipl...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000785

    authors: Duranti A,Sabbatucci M,Blasi G,Acciari VA,Ancora M,Bella A,Busani L,Centorame P,Cammà C,Conti F,De Medici D,Di Domenico M,Di Marzio V,Filippini G,Fiore A,Fisichella S,Gattuso A,Gianfranceschi M,Graziani C,Guidi F,

    更新日期:2018-09-01 00:00:00

  • Prevalence of Anaplasma phagocytophilum and its coinfection with Borrelia afzelii in Ixodes ricinus and Ixodes persulcatus ticks inhabiting Tver Province (Russia) - a sympatric region for both tick species.

    abstract::Human granulocytic anaplasmosis (HGA) and Lyme borreliosis (LB) are tick-borne infectious diseases caused by Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato species, respectively. In this study, p44/msp2 paralogues specific to A. phagocytophilum and 5S-23S rRNA gene-intergenic spacers specific to B. burg...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.47721-0

    authors: Masuzawa T,Kharitonenkov IG,Okamoto Y,Fukui T,Ohashi N

    更新日期:2008-08-01 00:00:00

  • Non-tuberculous mycobacterial infection of the parotid gland in an immunosuppressed adult.

    abstract::Infections of the parotid gland with non-tuberculous mycobacteria (NTM) are rarely described. Here, we report on an infection of the parotid gland caused by Mycobacterium avium and give a literature-based overview about this entity. In the light of a global increase of mycobacterial infections, unusual manifestations ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.47698-0

    authors: Gittinger FS,Raible A,Kempf VAJ

    更新日期:2008-04-01 00:00:00

  • Identification of Helicobacter in gastric biopsies by PCR based on 16S rDNA sequences: a matter of little significance for the prediction of H. pylori-associated gastritis?

    abstract::The aim of the present study was to correlate molecular evidence of the presence of Helicobacter pylori in gastric biopsy samples, based on analysis of 16S rDNA, vacuolating toxin (vacA), urease A (ureA) and cagA genes, with the clinical, histological and serological findings in patients with H. pylori-associated gast...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-47-8-695

    authors: Tiveljung A,Borch K,Jonasson J,Mårdh S,Petersson F,Monstein HJ

    更新日期:1998-08-01 00:00:00

  • Role of biofilm morphology, matrix content and surface hydrophobicity in the biofilm-forming capacity of various Candida species.

    abstract::The present study aimed to evaluate the role of biofilm morphology, matrix content and surface hydrophobicity in the biofilm-forming capacity of Candida albicans and non-albicans Candida (NAC) spp. Biofilm formation was determined by microtitre plate assay and bright-field and scanning electron microscopy. The matrix ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000747

    authors: Kumari A,Mankotia S,Chaubey B,Luthra M,Singh R

    更新日期:2018-06-01 00:00:00

  • Association of Clostridium difficile ribotype 078 with detectable toxin in human stool specimens.

    abstract::Using a Clostridium difficile glutamate dehydrogenase (GDH) immunoassay and a sensitive C. difficile toxin A/B immunoassay, human stool specimens from patients with diarrhoea (n = 1085) were classified as either GDH positive/toxin negative, or GDH positive/toxin positive. Overall, 528/725 (73%) of the GDH-positive/tox...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000165

    authors: Fairley DJ,McKenna JP,Stevenson M,Weaver J,Gilliland C,Watt A,Coyle PV

    更新日期:2015-11-01 00:00:00

  • Nasal colonization by four potential respiratory bacteria in healthy children attending kindergarten or elementary school in Seoul, Korea.

    abstract::A longitudinal analysis was carried out of the colonization by four potential respiratory pathogens - Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis and Staphylococcus aureus - in 165 healthy children (aged 3-7 years) attending three kindergartens and 417 healthy children (aged 7-10 years) att...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.040584-0

    authors: Bae S,Yu JY,Lee K,Lee S,Park B,Kang Y

    更新日期:2012-05-01 00:00:00

  • Development of a multiplex PCR assay to detect the major clonal complexes of Streptococcus suis relevant to human infection.

    abstract::Multilocus sequence typing (MLST) is considered a reliable method for providing insight into the Streptococcus suis population structure, clonal relationships and the potential of particular clones to cause disease. Indeed, MLST has revealed the presence of several clonal complexes (CCs) within the Streptococcus suis ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000239

    authors: Hatrongjit R,Kerdsin A,Gottschalk M,Hamada S,Oishi K,Akeda Y

    更新日期:2016-05-01 00:00:00

  • Seroprevalence of Chlamydia infection in pigs in Jiangxi province, South-Eastern China.

    abstract::Chlamydia are Gram-negative obligate bacteria that cause a wide range of diseases in humans and animals. To assess the risk of zoonosis posed by pigs, a total of 920 serum samples were collected from pigs in 11 administrative cities in Jiangxi province, south-eastern China, and the seroprevalence of Chlamydia antibodi...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.066555-0

    authors: Jiang HH,Huang SY,Zhang WB,Zhao L,Xu CM,Deng SZ,Zhu XQ

    更新日期:2013-12-01 00:00:00

  • Antibacterial activity of a 1,8-naphthyridine quinolone, PD 131628.

    abstract::A new 1,8-naphthyridine quinolone antimicrobial, PD131628, was found to be as, or more active than ciprofloxacin or ofloxacin against Escherichia coli and considerably more active than the two 4-quinolone agents against staphylococci in terms of both MIC and OBC (the optimum bactericidal concentration, at which the ra...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-36-5-353

    authors: Lewin CS

    更新日期:1992-05-01 00:00:00

  • Assessment of the clinical utility of serial beta-D-glucan concentrations in patients with persistent neutropenic fever.

    abstract::The performance of the Fungitell assay was investigated in 100 patients with haematological malignancy undergoing chemotherapy who developed antibiotic-unresponsive neutropenic fever (AUNF). Serum beta-D-glucan (BG) concentrations were significantly elevated on the first day of AUNF and all subsequent alternate days t...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.47479-0

    authors: Ellis M,Al-Ramadi B,Finkelman M,Hedstrom U,Kristensen J,Ali-Zadeh H,Klingspor L

    更新日期:2008-03-01 00:00:00

  • Effect of intensive handwashing in the prevention of diarrhoeal illness among patients with AIDS: a randomized controlled study.

    abstract::Patients with AIDS frequently develop diarrhoeal illness. In this randomized, controlled study, 260 patients were screened for those who had not had diarrhoea in the preceding 3 months and who had received a stable highly active antiretroviral therapy regimen for at least 6 weeks prior to the study enrollment. A total...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章,随机对照试验

    doi:10.1099/jmm.0.46867-0

    authors: Huang DB,Zhou J

    更新日期:2007-05-01 00:00:00

  • Liver abscess due to Neisseria sicca after repeated transcatheter arterial embolization.

    abstract::Neisseria sicca is rarely associated with clinical infections and to the authors' knowledge this organism has not been reported as a causative agent of infected biloma (liver abscess). A case of a diabetic man with infected biloma due to N. sicca after repeated transcatheter arterial embolization for hepatocellular ca...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.47192-0

    authors: Chung HC,Teng LJ,Hsueh PR

    更新日期:2007-11-01 00:00:00

  • Detection of species-specific and cross-reactive cell-surface antigens of Bacteroides species by an indirect enzyme-linked immunosorbent assay.

    abstract::EDTA-released outer-membrane antigen complexes were prepared from 172 laboratory and reference strains and 43 fresh clinical or faecal isolates representing 20 species or subspecies of Bacteroides, together with 13 species of other genera. These antigens were titrated against antisera to whole, live cells of 21 specie...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-15-2-223

    authors: Poxton IR,Brown R,Collee JG

    更新日期:1982-05-01 00:00:00

  • Comparison of four automated nucleic acid extraction platforms for the recovery of DNA from Aspergillus fumigatus.

    abstract::Invasive aspergillosis is a significant cause of morbidity and mortality within at-risk groups. Directed antifungal chemotherapy, guided by effective screening algorithms that incorporate reliable and validated molecular assays, reduces the morbidity associated with empirical administration and allows earlier diagnosi...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.076315-0

    authors: Perry MD,White PL,Barnes RA

    更新日期:2014-09-01 00:00:00