Abstract:
:Multilocus sequence typing (MLST) is considered a reliable method for providing insight into the Streptococcus suis population structure, clonal relationships and the potential of particular clones to cause disease. Indeed, MLST has revealed the presence of several clonal complexes (CCs) within the Streptococcus suis population. However, the method is costly, time-consuming and difficult to use for screening large numbers of isolates. In this study, a multiplex PCR assay was developed to identify Streptococcus suis CCs that are relevant to human infections. The multiplex PCR assay was capable of simultaneously distinguishing CC1, CC25, CC28, CC104, CC221/234 and CC233/379, which are related to human infections in Thailand, in a single reaction. The multiplex PCR assay is useful for low-cost screening of large numbers of isolates with rapid analytical capacity and could be utilized in most laboratories.
journal_name
J Med Microbioljournal_title
Journal of medical microbiologyauthors
Hatrongjit R,Kerdsin A,Gottschalk M,Hamada S,Oishi K,Akeda Ydoi
10.1099/jmm.0.000239subject
Has Abstractpub_date
2016-05-01 00:00:00pages
392-396issue
5eissn
0022-2615issn
1473-5644journal_volume
65pub_type
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