Molecular epidemiology of an outbreak of multiresistant Klebsiella pneumoniae in a Tunisian neonatal ward.

abstract：:The ability of various haem- and non-haem-iron-containing compounds to support the growth of iron-limited cultures of Haemophilus ducreyi was assessed in a plate bioassay. Only haemin or the haem-containing proteins, bovine haemoglobin, human haemoglobin and bovine catalase, but not equine cytochrome C111, were capabl...

journal_title：Journal of medical microbiology

authors： Lee BC

更新日期：1991-06-01 00:00:00

abstract：:Experiments were performed to determine whether a modern flow cytometer could be used to study bacterial populations in suspension, with particular reference to their morphological characteristics and their responses to antibiotics. The FACScan, a commercial benchtop flow cytometer fitted with an air-cooled laser, des...

journal_title：Journal of medical microbiology

authors： Gant VA,Warnes G,Phillips I,Savidge GF

更新日期：1993-08-01 00:00:00

abstract：:The role of Bacteroides fragilis in the pathogenesis of acute appendicitis was studied in 135 patients in four patient groups: normal (17); phlegmonous appendicitis (17); gangrenous appendicitis (75); and septic complications of appendicitis (26). Aerobic and anaerobic bacteria were isolated from all groups and member...

journal_title：Journal of medical microbiology

authors： Elhag KM,Alwan MH,Al-Adnani MS,Sherif RA

更新日期：1986-05-01 00:00:00

abstract：:PCR has proved superior to conventional blood culture for diagnosing bacteraemia in the presence of antibiotics. Nevertheless, even PCR might yield false-negative results if the template DNA were to be cleaved by serum DNAases after antibiotics had induced bacterial death. To evaluate the cleavage of bacterial templat...

journal_title：Journal of medical microbiology

authors： Heininger A,Ulrich M,Priebe G,Unertl K,Müller-Schauenburg A,Botzenhart K,Döring G

更新日期：2001-03-01 00:00:00

abstract：:The effects of ethyleneglycoltetra-acetic acid (EGTA) and EGTA + magnesium (MgEGTA) on the viable counts of 10 strains of Escherichia coli O6 have been studied in normal human serum (NHS), heat-inactivated serum (HIS) and in culture media with and without the addition of a beta-lactam antibiotic. The addition of EGTA ...

journal_title：Journal of medical microbiology

authors： Roberts AP,Phillips R

更新日期：1981-05-01 00:00:00

abstract：:A total of 36 Escherichia coli and 31 enterococci isolates were recovered from 42 common buzzard faecal samples. The E. coli isolates showed high levels of resistance to streptomycin and tetracycline. The following resistance genes were detected: bla(TEM) (20 of 22 ampicillin-resistant isolates), tet(A) and/or tet(B) ...

journal_title：Journal of medical microbiology

authors： Radhouani H,Poeta P,Gonçalves A,Pacheco R,Sargo R,Igrejas G

更新日期：2012-06-01 00:00:00

abstract：:Introduction. Ocular microsporidiosis is a significant emerging infectious disease reported in immunocompromised patients and immunocompetent persons throughout the world.Aim. To identify the pathogens responsible for human keratitis, via corneal scrapings.Methodology. Thirty-three hospitalized patients with epithelia...

journal_title：Journal of medical microbiology

authors： Sanpool O,Thanathanee O,Laummuanwai P,Maleewong W,Intapan PM

更新日期：2020-03-01 00:00:00

abstract：:In the present study, polyphasic analysis [cultivation, combined with the fingerprinting of individual isolates, and denaturing gradient gel electrophoresis (DGGE)] was applied to study whether similar features concerning the diversity and temporal stability of selected bacterial groups could be detected intra-individ...

journal_title：Journal of medical microbiology

authors： Maukonen J,Mättö J,Suihko ML,Saarela M

更新日期：2008-12-01 00:00:00

abstract：:Epidemic methicillin-resistant Staphylococcus aureus types 15 and 16 (EMRSA-15 and EMRSA-16) are the dominant types of MRSA found in UK hospitals, but accurate designation of strains has been difficult. Restriction fragment length polymorphism (RFLP) profiles of seven core virulence genes were used to classify unambig...

journal_title：Journal of medical microbiology

authors： Moore PCL,Lindsay JA

更新日期：2002-06-01 00:00:00

abstract：:A porous inorganic material (Anopore) was employed as a microbial culture and microcolony imaging support. Rapid Anopore-based antibiotic sensitivity testing (AST) methods were developed to assess the growth of clinical isolates, with the primary focus on testing the response of the Enterobacteriaceae to trimethoprim,...

journal_title：Journal of medical microbiology

authors： Ingham CJ,van den Ende M,Wever PC,Schneeberger PM

更新日期：2006-11-01 00:00:00

abstract：:Amplification and sequence analysis of the 16S rRNA gene can be applied to detect and identify bacteria in clinical samples. We examined 75 clinical samples (17 culture-positive, 58 culture-negative) prospectively by two different PCR protocols, amplifying either a single fragment (1343 bp) or two fragments (762/598 b...

journal_title：Journal of medical microbiology

authors： Jenkins C,Ling CL,Ciesielczuk HL,Lockwood J,Hopkins S,McHugh TD,Gillespie SH,Kibbler CC

更新日期：2012-04-01 00:00:00

abstract：:Yersinia pseudotuberculosis was examined for its haemagglutinating activity and adherence to cultured epithelial cells (HEp-2) in relation to possession of a virulence (VW) plasmid and to growth conditions. VW-lacking (VW-) bacteria were isolated from ten VW+ strains of each serovar which, after they were grown on CFA...

journal_title：Journal of medical microbiology

authors： Kawaoka Y,Mitani T,Hoshina K,Otsuki K,Tsubokura M

更新日期：1988-03-01 00:00:00

abstract：:Helicobacter pylori and Proteus mirabilis ureases are nickel-requiring metallo-enzymes that hydrolyse urea to NH3 and CO2. In both H. pylori and in an Escherichia coli model of H. pylori urease activity, a high affinity nickel transporter, NixA, is required for optimal urease activity, whereas the urea-dependent UreR ...

journal_title：Journal of medical microbiology

authors： McGEE DJ,Coker C,Testerman TL,Harro JM,Gibson SV,Mobley HLT

更新日期：2002-11-01 00:00:00

abstract：:The biochemical characteristics, enterotoxigenicity and susceptibility to antibiotics are reported for 22 strains of Aeromonas species isolated from clinical specimens in the Western Region of Saudi Arabia. Aeromonas caviae was the species most frequently observed; a high proportion of these strains fermented lactose,...

journal_title：Journal of medical microbiology

authors： Gosling PJ

更新日期：1986-08-01 00:00:00

abstract：:A collection of 198 clinical isolates of strains belonging to the tribe Proteeae was examined for haemolytic activity on blood agar and in Brain Heart Infusion Broth. The strains were of diverse bacteriocin and O-antigenic types and from a wide variety of sources. They included representatives of all species of Morgan...

journal_title：Journal of medical microbiology

authors： Senior BW,Hughes C

更新日期：1988-01-01 00:00:00

abstract：:A total of 110 clinical isolates of Vibrio cholerae O1 biotype El Tor serotype Ogawa isolated in a recent outbreak from different districts of Teheran, Iran, was subjected to 99 carbon source utilisation tests, ribotyping and toxinogenotyping. PCR showed that the genes encoding cholera toxin (ctxA), toxin co-regulated...

journal_title：Journal of medical microbiology

authors： Pourshafie MR,Grimont F,Saifi M,Grimont PAD

更新日期：2000-12-01 00:00:00

abstract：:Competition, competitive exclusion and displacement of eight strains of Escherichia coli and Salmonella spp. by Lactobacillus rhamnosus GG and Lactobacillus casei Shirota from adhesion on human intestinal mucus glycoproteins and Caco-2 cell surfaces were studied. Lactobacilli were able to compete with, exclude and dis...

journal_title：Journal of medical microbiology

authors： Lee YK,Puong KY,Ouwehand AC,Salminen S

更新日期：2003-10-01 00:00:00

abstract：:A novel aminopeptidase was purified by high performance liquid chromatography from a cytosoluble 100,000 g extract of Candida albicans on the basis of its ability to cleave L-arginine 7-amino-4-methylcoumarin. The purification factor was 36 and the yield was 20%. The native enzyme had a mol. wt of 52 kDa as demonstrat...

journal_title：Journal of medical microbiology

authors： el Moudni B,Rodier MH,Barrault C,Ghazali M,Jacquemin JL

更新日期：1995-10-01 00:00:00

abstract：:To obtain monoclonal antibodies (MAbs) directed preferentially against the pathogenic phase of Candida albicans, mice were immunised with germ tubes of C. albicans serotype A, strain VW.32, killed by exposure to ultraviolet (UV) irradiation. Fusions were performed either by the standard chemical procedure with polyeth...

journal_title：Journal of medical microbiology

authors： Fortier B,Hopwood V,Poulain D

更新日期：1988-12-01 00:00:00

abstract：PURPOSE:Extracellular protein toxins contribute to the pathogenesis of Staphylococcus aureus infections. The present study compared the effects of iclaprim and trimethoprim - two folic acid synthesis inhibitors - with nafcillin and vancomycin on production of Panton-Valentine leukocidin (PVL), alpha haemolysin (AH) and...

journal_title：Journal of medical microbiology

authors： Bryant AE,Gomi S,Katahira E,Huang DB,Stevens DL

更新日期：2019-03-01 00:00:00

abstract：:Three specimens from 111 asymptomatic male partners of infertile couples attending the Department of Urology in Amiens, France, were examined by the PCR COBAS AMPLICOR test (Roche Molecular Diagnostics) for the presence of Chlamydia trachomatis. The specimens analysed were: first void urine (FVU), urine obtained after...

journal_title：Journal of medical microbiology

authors： Hamdad-Daoudi F,Petit J,Eb F

更新日期：2004-10-01 00:00:00

abstract：:The present study aimed to characterize cell damage caused by vaginal Candida albicans isolates from women with different symptomatologies. It was evaluated 12 clinical isolates of C. albicans from vaginal samples: 4 from asymptomatic women (AS), 4 from women with a single episode of vulvovaginal candidiasis (VVC) and...

journal_title：Journal of medical microbiology

authors： Faria DR,Sakita KM,Akimoto-Gunther LS,Kioshima ÉS,Svidzinski TIE,Bonfim-Mendonça PS

更新日期：2017-08-01 00:00:00

abstract：:We studied in vitro ceftaroline combinations against 61 meticillin-resistant Staphylococcus aureus isolates; 18 of them were also resistant to linezolid, using overlapping E-test method. Daptomycin-ceftaroline combination obtained lower fractional inhibitory concentration values, in comparison with those including van...

journal_title：Journal of medical microbiology

authors： García AB,Candel FJ,López L,Chiarella F,Viñuela-Prieto JM

更新日期：2016-10-01 00:00:00

abstract：:Ruminants are a major reservoir of enterohaemorrhagic Escherichia coli (EHEC), which cause acute gastroenteritis in humans with potentially life-threatening sequelae. The mechanisms underlying EHEC persistence in ruminant hosts are poorly understood. EHEC produce several cytotoxins that inhibit the proliferation of bo...

journal_title：Journal of medical microbiology

authors： Menge C,Stamm I,van Diemen PM,Sopp P,Baljer G,Wallis TS,Stevens MP

更新日期：2004-06-01 00:00:00

abstract：:The fungal pathogen Cryptococcus neoformans has a predilection for the central nervous system (CNS), resulting in devastating meningoencephalitis. At present, it is unclear how C. neoformans traverses the blood-brain barrier (BBB) and causes CNS infection. The present study has examined and characterized the interacti...

journal_title：Journal of medical microbiology

authors： Chen SHM,Stins MF,Huang SH,Chen YH,Kwon-Chung KJ,Chang Y,Kim KS,Suzuki K,Jong AY

更新日期：2003-11-01 00:00:00

abstract：PURPOSE:An observational study was performed to investigate the carriage rate and serotypes of Streptococcus pneumoniae in the 13-valent pneumococcal conjugate vaccine (PCV13) era in Taiwan. METHODOLOGY:From March 2014 to March 2015 a total of 500 healthy children and their households (631 adults) were enrolled from t...

journal_title：Journal of medical microbiology

authors： Janapatla RP,Su LH,Chen HH,Chang HJ,Tsai TC,Chen PY,Chen CL,Chiu CH

更新日期：2017-06-01 00:00:00

abstract：PURPOSE:Persister cells, a subpopulation of tolerant cells within the bacterial culture, are commonly thought to be responsible for antibiotic therapy failure and infection recurrence. Klebsiella pneumoniae is a notorious human pathogen for its increasing resistance to antibiotics and wide involvement in severe infecti...

journal_title：Journal of medical microbiology

authors： Li Y,Zhang L,Zhou Y,Zhang Z,Zhang X

更新日期：2018-03-01 00:00:00

abstract：:The sensitivity of Escherichia coli strains to 80% normal human serum (NHS) and the relative importance of the classical and alternative complement pathways was assessed in relation to K1, K5, and O antigen carriage. Strains of each of the common O-serogroups, O1, O2, O4, O6, O7, O9, O18 and O75, smooth strains not ty...

journal_title：Journal of medical microbiology

authors： Devine DA,Roberts AP

更新日期：1994-08-01 00:00:00

abstract：:A non-tuberculous mycobacterium was isolated, following a vertebral needle aspiration, from the blood of a patient with severe spondylodiscitis. The strain turned out to be different from any known mycobacterial species and was quite drug-susceptible in vitro. The patient improved markedly following treatment with mer...

journal_title：Journal of medical microbiology

authors： Tortoli E,Mantella A,Mariottini A,Mazzarelli G,Pecile P,Rogasi PG,Sterrantino G,Fantoni E,Leoncini F

更新日期：2006-01-01 00:00:00

abstract：:Yeast identification and in vitro susceptibility testing provide helpful information for appropriate administration of antifungal treatments; however, few reports from the Latin American region have been published. The aim of this study was to identify the species present in isolates from bloodstream infections diagno...

journal_title：Journal of medical microbiology

authors： Bustamante B,Martins MA,Bonfietti LX,Szeszs MW,Jacobs J,Garcia C,Melhem MSC

更新日期：2014-06-01 00:00:00