Molecular characterisation of the dominant UK methicillin-resistant Staphylococcus aureus strains, EMRSA-15 and EMRSA-16.

abstract：:Nosocomial infections with enterococci are an increasing problem in modern medical practice due to the development of resistance to a wide range of antibiotics, including the glycopeptides vancomycin and teicoplanin. An increasing number of vancomycin-resistant enterococci (VRE) have been cultured from clinical specim...

journal_title：Journal of medical microbiology

authors： Mackenzie CR,Hucke C,MÜller D,Seidel K,Takikawa O,DÄubener W

更新日期：1999-10-01 00:00:00

abstract：:Pseudomonas aeruginosa is an opportunistic human pathogen that causes serious and chronic infections. Many secondary metabolites are secreted throughout its growth, among which phenazine is a known virulence factor and signalling molecule. Phenazine is coordinately controlled by the global regulatory quorum-sensing (Q...

journal_title：Journal of medical microbiology

authors： Liang H,Duan J,Sibley CD,Surette MG,Duan K

更新日期：2011-01-01 00:00:00

abstract：:The growth of five species of Bacteroides in four anaerobic culture media was continuously monitored turbidimetrically. Interspecies differences were observed in the growth of Bacteroides spp. in the various media, but growth in Brain Heart Infusion broth supplemented with yeast extract, haemin and menadione, was cons...

journal_title：Journal of medical microbiology

authors： Eley A,Greenwood D,O'Grady F

更新日期：1985-04-01 00:00:00

abstract：:Rapid identification of the causative bacteria of sepsis in patients can contribute to the selection of appropriate antibiotics and improvement of patients' prognosis. Genotypic identification is an emerging technology that may provide an alternative method to, or complement, established phenotypic identification proc...

journal_title：Journal of medical microbiology

authors： Motoshima M,Yanagihara K,Morinaga Y,Matsuda J,Hasegawa H,Kohno S,Kamihira S

更新日期：2012-11-01 00:00:00

abstract：:Rickettsia rickettsii has limited adverse effects on its arthropod vector, but causes severe disease in man. To model differences in host-parasite interaction, R. rickettsii growth and protein expression were examined at temperatures reflective of host environment in the tick cell lines DALBE3 and IDE2, the human endo...

journal_title：Journal of medical microbiology

authors： Policastro PF,Munderloh UG,Fischer ER,Hackstadt T

更新日期：1997-10-01 00:00:00

abstract：:Effects on growth and toxin A production of sub-MIC concentrations of six different antibiotics were investigated in three strains of Clostridium difficile: reference strain NCTC 11223, a fully sequenced strain (630) and a locally endemic isolate (strain 338a). The antibiotics chosen for investigation were the agents ...

journal_title：Journal of medical microbiology

authors： Drummond LJ,Smith DGE,Poxton IR

更新日期：2003-12-01 00:00:00

abstract：:Enteropathogenic Escherichia coli (EPEC) is an important aetiological agent of persistent infantile diarrhoea. EPEC pathogenicity is not mediated through known toxins and the role played by outer-membrane proteins (OMPs) in the initial adherence of the bacterium, intimate attachment to epithelial cells and ultimately ...

journal_title：Journal of medical microbiology

authors： Kumar SS,Sankaran K,Haigh R,Williams PH,Balakrishnan A

更新日期：2001-07-01 00:00:00

abstract：:Resistance patterns and carbapenemase gene presence among Klebsiella pneumoniae isolates from the University General Hospital of Patras, Greece during a ten-year period were analysed under a surveillance programme for multi-drug-resistant bacteria. From 2005 to 2014, K. pneumoniae isolates from clinically significant ...

journal_title：Journal of medical microbiology

authors： Spyropoulou A,Papadimitriou-Olivgeris M,Bartzavali C,Vamvakopoulou S,Marangos M,Spiliopoulou I,Anastassiou ED,Christofidou M

更新日期：2016-03-01 00:00:00

abstract：PURPOSE:We resequenced the genome of Clostridium difficile 630Δerm (DSM 28645), a model strain commonly used for the generation of insertion mutants. METHODOLOGY:The genome sequence was obtained by a combination of single-molecule real-timeand Illumina sequencing technology. RESULTS:Detailed manual curation and compa...

journal_title：Journal of medical microbiology

authors： Dannheim H,Riedel T,Neumann-Schaal M,Bunk B,Schober I,Spröer C,Chibani CM,Gronow S,Liesegang H,Overmann J,Schomburg D

更新日期：2017-03-01 00:00:00

abstract：PURPOSE:This study investigates the virulence and antimicrobial resistance in association with common clonal complexes (CCs) of enteroaggregative Escherichia coli (EAEC) isolated from Bangladesh. The aim was to determine whether specific CCs were more likely to be associated with putative virulence genes and/or antimic...

journal_title：Journal of medical microbiology

authors： Chattaway MA,Day M,Mtwale J,White E,Rogers J,Day M,Powell D,Ahmad M,Harris R,Talukder KA,Wain J,Jenkins C,Cravioto A

更新日期：2017-10-01 00:00:00

abstract：PURPOSE:Non-albicansCandida species have emerged as fungal pathogens that cause invasive infections, with many of these species displaying resistance to commonly used antifungal agents. This study was confined to studying the characteristics of clinical isolates of the C. rugosa complex and C. pararugosa species. METH...

journal_title：Journal of medical microbiology

authors： Peremalo T,Madhavan P,Hamzah S,Than L,Wong EH,Nasir MDM,Chong PP,Ng KP

更新日期：2019-03-01 00:00:00

abstract：:Chlamydia are Gram-negative obligate bacteria that cause a wide range of diseases in humans and animals. To assess the risk of zoonosis posed by pigs, a total of 920 serum samples were collected from pigs in 11 administrative cities in Jiangxi province, south-eastern China, and the seroprevalence of Chlamydia antibodi...

journal_title：Journal of medical microbiology

authors： Jiang HH,Huang SY,Zhang WB,Zhao L,Xu CM,Deng SZ,Zhu XQ

更新日期：2013-12-01 00:00:00

abstract：:Cell walls from different species of Mycobacterium were purified on a sucrose step gradient. The components derived from these preparations were characterised by sodium dodecyl sulphate-polyacrylamide gel electrophoresis, followed by staining or by Western blotting. Surface-exposed polypeptide molecules were also iden...

journal_title：Journal of medical microbiology

authors： Babu S,Jain NK,Prasad G,Bhattacharya S,Bhattacharya A

更新日期：1990-08-01 00:00:00

abstract：:Chlamydia trachomatis results in tubal factor infertility in some women. Diagnosis of this tubal infertility is difficult and typically involves laparoscopy or hysterosalpingography to detect the tubal blockages. Numerous serological tests have been developed; however, they are presently not used for diagnosis without...

journal_title：Journal of medical microbiology

authors： Menon S,Stansfield SH,Logan B,Hocking JS,Timms P,Rombauts L,Allan JA,Huston WM

更新日期：2016-09-01 00:00:00

abstract：:During a one-year period, 258 isolates of Campylobacter jejuni and C. coli were obtained from children with gastroenteritis or bacteraemia at the Red Cross Children's Hospital, Cape Town, South Africa. These isolates were biotyped by hippurate hydrolysis, H2S production and tolerance to 2,3,5-triphenyltetrazolium chlo...

journal_title：Journal of medical microbiology

authors： Lastovica AJ,Le Roux E,Congi RV,Penner JL

更新日期：1986-02-01 00:00:00

abstract：:Phenotypic loss of protein A production was tested in six methicillin-resistant (McR) Staphylococcus aureus (MRSA) isolates and their isogenic methicillin-sensitive (McS) variants by a radiolabelled IgG-binding assay with washed cells and by Western blotting of supernates prepared from lysed washed cells. Genomic DNA ...

journal_title：Journal of medical microbiology

authors： Poston SM,Glancey GR,Wyatt JE,Hogan T,Foster TJ

更新日期：1993-12-01 00:00:00

abstract：:Experiments were performed to determine whether a modern flow cytometer could be used to study bacterial populations in suspension, with particular reference to their morphological characteristics and their responses to antibiotics. The FACScan, a commercial benchtop flow cytometer fitted with an air-cooled laser, des...

journal_title：Journal of medical microbiology

authors： Gant VA,Warnes G,Phillips I,Savidge GF

更新日期：1993-08-01 00:00:00

abstract：:Pyrolysis mass spectrometry (PyMS), a highly discriminatory method for comparison of isolates, was used to assess the homogeneity of colonies taken from apparently pure cultures of Escherichia coli in 10 urine specimens. Ten randomly selected colonies were subcultured from each urine for comparison. For six urines, th...

journal_title：Journal of medical microbiology

authors： Freeman R,Sisson PR,Burdess D

更新日期：1996-11-01 00:00:00

abstract：:An outer-membrane protein (OMP) was isolated from a clinical strain of Bacteroides distasonis. Changes in growth media did not appreciably affect the appearance of this protein in crude outer-membrane preparations examined by SDS-PAGE. However, the proportion of the protein relative to other OMPs was greater in 24-h c...

journal_title：Journal of medical microbiology

authors： Wexler HM,Getty C,Fisher G

更新日期：1992-09-01 00:00:00

abstract：:Introduction. Quantification of bacterial load in tissue homogenates in in vivo pharmacodynamic studies is cumbersome and time-consuming.Aim. We therefore developed a new method for quantifying bacterial load in tissue homogenates of animals treated with a β-lactam and β-lactamase inhibitor using growth curves.Methods...

journal_title：Journal of medical microbiology

authors： Georgiou PC,Mouton JW,Pournaras S,Meletiadis J

更新日期：2020-05-01 00:00:00

abstract：PURPOSE:This study aimed to investigate the effect of smoking on the buccal microbiome and to analyse the descriptive ability of each of the seven hypervariable regions in their 16S rRNA genes. METHODOLOGY:Microbiome compositions of 40 buccal swab samples collected from smokers (n =20) and non-smokers (n =20) were det...

journal_title：Journal of medical microbiology

authors： Karabudak S,Ari O,Durmaz B,Dal T,Basyigit T,Kalcioglu MT,Durmaz R

更新日期：2019-08-01 00:00:00

abstract：:Clostridium difficile is the most common cause of antibiotic-associated diarrhoea. Antibiotics are presumed to disturb the normal intestinal microbiota, leading to depletion of the barrier effect and colonization by pathogenic bacteria. This first step of infection includes adherence to epithelial cells. We investigat...

journal_title：Journal of medical microbiology

authors： Denève C,Deloménie C,Barc MC,Collignon A,Janoir C

更新日期：2008-06-01 00:00:00

abstract：:The distribution of hepatitis C virus (HCV) genotypes among Egyptian patients positive for anti-HCV was determined and their influence, when combined with neu-oncoprotein overexpression, on the development of hepatocellular carcinoma (HCC) was examined. The study groups included asymptomatic carriers (ASC) and patient...

journal_title：Journal of medical microbiology

authors： Zekri AN,Bahnassy AA,Shaarawy SM,Mansour OA,Maduar MA,Khaled HM,El-Ahmadi O

更新日期：2000-01-01 00:00:00

abstract：:Of 200 isolates of Vibrio mimicus screened, one from water (N-57) agglutinated with V. cholerae O139 polyclonal antiserum (absorbed with a rough strain of V. cholerae only) and not with O139 polyclonal diagnostic antiserum (absorbed with the rough strain and V. cholerae O22 and O155). The antigenic relationship betwee...

journal_title：Journal of medical microbiology

authors： Ansaruzzaman M,Shimada T,Bhuiyan NA,Nahar S,Alam K,Islam MS,Albert MJ

更新日期：1999-09-01 00:00:00

abstract：:Extracts of 19 samples of pus which showed red fluorescence with ultraviolet light were screened for the presence of porphyrins by absorption spectrophotometry. All those which showed spectra typical of metal-free porphyrins were analysed by high performance liquid chromatography to identify the porphyrins present. Th...

journal_title：Journal of medical microbiology

authors： Brazier JS

更新日期：1990-09-01 00:00:00

abstract：:The nasal carrier-rate of Staphylococcus aureus in 548 Nigerians aged 9-32 years and attending various educational establishments was 56.4%. This rate decreased with increasing age. A significantly greater proportion of females (65.0%) than males (46.5%) were carriers, but the excess in females was apparent only in su...

journal_title：Journal of medical microbiology

authors： Lamikanra A,Paul BD,Akinwole OB,Paul MO

更新日期：1985-04-01 00:00:00

abstract：:In tests of bactericidal action against H. influenzae type b strains isolated from patients with meningitis, chloramphenicol was found to be far more reliable than ampicillin in dealing with large inocula, and more rapidly effective against both large and relatively small inocula. These findings provide a laboratory e...

journal_title：Journal of medical microbiology

authors： Turk DC

更新日期：1977-02-01 00:00:00

abstract：:A sandwich enzyme-linked immunosorbent assay (ELISA) with monoclonal antibody coupled to the solid phase was evaluated for the detection of brucella antigen in serum samples. Under optimum conditions, 100 brucella cells/well or 10(5) fg/ml of lipopolysaccharide (LPS) were detected in spiked specimens. The standardised...

journal_title：Journal of medical microbiology

authors： Al-Shamahy HA,Wright SG

更新日期：1998-02-01 00:00:00

abstract：:The ability of Proteus mirabilis to produce the bacteriocin, proticine 3, is found almost exclusively in strains that cause severe infections of the upper urinary tract. Proticine 3 was purified from lysates of mitomycin C-induced cultures. Biological activity was associated with structures resembling bacteriophage ta...

journal_title：Journal of medical microbiology

authors： Senior BW

更新日期：1983-08-01 00:00:00

abstract：:The ELISA technique was shown to be group-specific for the detection of IgM antibodies against coxsackie B viruses, and probably against a wider range of enteroviruses. No evidence was obtained that recent coxsackie B-virus infection predisposes to myocardial infarction. ...

journal_title：Journal of medical microbiology

authors： Hannington G,Booth JC,Bowes RJ,Stern H

更新日期：1986-06-01 00:00:00