Molecular characterisation of the dominant UK methicillin-resistant Staphylococcus aureus strains, EMRSA-15 and EMRSA-16.


:Epidemic methicillin-resistant Staphylococcus aureus types 15 and 16 (EMRSA-15 and EMRSA-16) are the dominant types of MRSA found in UK hospitals, but accurate designation of strains has been difficult. Restriction fragment length polymorphism (RFLP) profiles of seven core virulence genes were used to classify unambiguously isolates of MRSA from St George's Hospital into two groups corresponding to EMRSA-15 and EMRSA-16. Variants of both EMRSA-15 and EMRSA-16 isolates occurred that had lost virulence genes encoded on mobile genetic elements. EMRSA-16 isolates had core gene profiles identical to a cluster of previously characterised MSSA (methicillin-sensitive S. aureus) isolates from St George's Hospital, suggesting that they have arisen from this source, or that loss of the accessory genetic element encoding methicillin resistance is frequent. EMRSA-15 and EMRSA-16 strains were distinct from other MRSA strains previously identified in UK hospitals, and always carried a mobile genetic element encoding multiple superantigens. These results contribute to the understanding of the types of MRSA found in UK hospitals, how they vary and how they arose.


J Med Microbiol


Moore PCL,Lindsay JA





Has Abstract


2002-06-01 00:00:00












  • Detection of species-specific and cross-reactive cell-surface antigens of Bacteroides species by an indirect enzyme-linked immunosorbent assay.

    abstract::EDTA-released outer-membrane antigen complexes were prepared from 172 laboratory and reference strains and 43 fresh clinical or faecal isolates representing 20 species or subspecies of Bacteroides, together with 13 species of other genera. These antigens were titrated against antisera to whole, live cells of 21 specie...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Poxton IR,Brown R,Collee JG

    更新日期:1982-05-01 00:00:00

  • Successful substitution of fetal calf serum by human plasma for bulk cultivation of Leishmania donovani promastigotes.

    abstract::The potential of human plasma (HP) or serum (HS) as a replacement for fetal calf serum (FCS) was evaluated in a liver infusion tryptose (LIT) medium for bulk cultivation of Leishmania donovani promastigotes. The promastigote yield with the LIT-FCS standard medium was 0.4-1.8×10(7) ml(-1), and yields of 0.5-3.4×10(7) (...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Mahamoud A,Osman HA,Mansour D,El Harith A

    更新日期:2013-08-01 00:00:00

  • Influence of Campylobacter fetus subsp. fetus on ram sperm cell quality.

    abstract::Campylobacter fetus subsp. fetus infection can occur in female sheep, causing infertility or abortion. Despite extensive research on the effect of these bacteria on female fertility, little research has been done on the influence of C. fetus subsp. fetus on the male factor. Our objective was to examine the influence o...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Zan Bar T,Yehuda R,Hacham T,Krupnik S,Bartoov B

    更新日期:2008-11-01 00:00:00

  • Growth of Legionella spp. under conditions of iron restriction.

    abstract::The growth inhibiting activity of transferrins, citrate, 2-2' dipyridyl and desferrioxamine methanesulphonate towards Legionella spp. and their serogroups was investigated. The inhibitory activity of all these compounds depended upon the iron-free state of the molecules and was abolished by saturation with iron. No ba...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Goldoni P,Visca P,Pastoris MC,Valenti P,Orsi N

    更新日期:1991-02-01 00:00:00

  • Klebsiella pneumoniae meningitis induces memory impairment and increases pro-inflammatory host response in the central nervous system of Wistar rats.

    abstract::Klebsiella pneumoniae meningitis has recently become an increasingly common cause of central nervous system infection. The invasion of bacteria within the subarachnoid space stimulates the release of pro-inflammatory cytokines and chemokines, triggering a host immune response. The aim of the present study was to evalu...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Barichello T,Simões LR,Valvassori SS,Generoso JS,Aveline PEDV,Dominguini D,Elias SG,Vilela MC,Quevedo J,Teixeira AL

    更新日期:2014-01-01 00:00:00

  • Agglutination of "Streptococcus milleri" by lectins.

    abstract::The agglutination of 218 clinical isolates and three ATCC type strains of "Streptococcus milleri" was tested with 25 different lectins from plants and fungi. An agglutination reaction with one or more lectins was observed with 42 isolates when the cells were untreated. After trypsinisation of the bacteria, 109 strains...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Kellens JT,Jacobs JA,Peumans WJ,Stobberingh EE

    更新日期:1994-07-01 00:00:00

  • Accuracy of in vitro susceptibility tests for carbapenemase-producing Gram-negative bacteria.

    abstract::Accurate susceptibility results on antibiotic-resistant bacteria are essential for proper treatment of infections. In this study, 100 metallo-β-lactamase (MBL)-producing strains and 95 isolates with Klebsiella pneumoniae carbapenemase (KPC) were tested for carbapenem susceptibility using two automated platforms, the P...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Sun J,Xu Y,Yu Y,Ni Y

    更新日期:2015-06-01 00:00:00

  • Non-tuberculous mycobacterial infection of the parotid gland in an immunosuppressed adult.

    abstract::Infections of the parotid gland with non-tuberculous mycobacteria (NTM) are rarely described. Here, we report on an infection of the parotid gland caused by Mycobacterium avium and give a literature-based overview about this entity. In the light of a global increase of mycobacterial infections, unusual manifestations ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Gittinger FS,Raible A,Kempf VAJ

    更新日期:2008-04-01 00:00:00

  • Effect of inhibitors in clinical specimens on Taq and Tth DNA polymerase-based PCR amplification of influenza A virus.

    abstract::Fifteen randomly selected nasopharyngeal (NP) swab specimens (culture-negative for influenza A virus) were spiked with influenza A virus and the nucleic acids were extracted and subjected to PCR amplification with Thermus aquaticus (Taq) and T. thermophilus (Tth) DNA polymerases. Products of the expected size, and giv...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Poddar SK,Sawyer MH,Connor JD

    更新日期:1998-12-01 00:00:00

  • Demonstration of spirochaetes in patients with Lyme disease with a modified silver stain.

    abstract::Spirochaetes were demonstrated in material from patients with Lyme disease by short-time high-concentrate silver impregnation after treatment with amylase. Removal of mucoid material was essential to visualise Borrelia burgdorferi. Lyme spirochaetes were demonstrated in material from 23 patients with Lyme disease--ery...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: De Koning J,Bosma RB,Hoogkamp-Korstanje JA

    更新日期:1987-05-01 00:00:00

  • Cross-reaction between a strain of Vibrio mimicus and V. cholerae O139 Bengal.

    abstract::Of 200 isolates of Vibrio mimicus screened, one from water (N-57) agglutinated with V. cholerae O139 polyclonal antiserum (absorbed with a rough strain of V. cholerae only) and not with O139 polyclonal diagnostic antiserum (absorbed with the rough strain and V. cholerae O22 and O155). The antigenic relationship betwee...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Ansaruzzaman M,Shimada T,Bhuiyan NA,Nahar S,Alam K,Islam MS,Albert MJ

    更新日期:1999-09-01 00:00:00

  • Analysis of Epstein-Barr virus and cellular gene expression during the early phases of Epstein-Barr virus lytic induction.

    abstract::In order to develop novel host/pathogen real-time PCR assays for routine diagnostic use, early gene expression patterns from both Epstein-Barr virus (EBV) and Raji cells were examined after inducing the lytic life cycle using 12-O-tetradecanoyl-13-phorbol ester and sodium butyrate. Real-time PCR identified several hig...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Auburn H,Zuckerman M,Smith M

    更新日期:2016-11-01 00:00:00

  • Extended phage locus typing of Salmonella enterica serovar Typhimurium, using multiplex PCR-based reverse line blot hybridization.

    abstract::Salmonella enterica serovar Typhimurium (S. Typhimurium) is the commonest pathogen causing food-borne disease among humans and animals in Australia. A multiplex PCR-based reverse line blot (mPCR/RLB) system was developed to rapidly identify S. Typhimurium phage types and strains within them. The system comprised 32 bi...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Wang Q,Kong F,Jelfs P,Gilbert GL

    更新日期:2008-07-01 00:00:00

  • Pleuritis due to Brevundimonas diminuta in a previously healthy man.

    abstract::Brevundimonas diminuta is rarely associated with invasive infections. We report the case of a previously healthy young man with pleural effusion, in which B. diminuta was recovered but incorrectly identified as Kingella kingae when it was freshly isolated. Consequently, the misidentification resulted in initial treatm...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Lu B,Shi Y,Zhu F,Xu X

    更新日期:2013-03-01 00:00:00

  • The routine application of a microtechnique for the demonstration of leptospiral antibodies.

    abstract::The microtechnique of Galton et al. (1965) for the routine screening of blood samples for leptospiral antibodies was used to examine more than 8000 serum samples from animals that had aborted and from herds in which leptospiral antibodies had previously been demonstrated. The method gave consistently accurate results....

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Lewis J

    更新日期:1978-08-01 00:00:00

  • Clioquinol is a promising preventive morphological switching compound in the treatment of Candida infections linked to the use of intrauterine devices.

    abstract:PURPOSE:Candida biofilm infections are frequently linked to the use of biomaterials and are of clinical significance because they are commonly resistant to antifungals. Clioquinol is an antiseptic drug and is effective against multidrug-resistant Candida. We investigated the effect of clioquinol and two other 8-hydroxy...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Pippi B,Machado GDRM,Bergamo VZ,Alves RJ,Andrade SF,Fuentefria AM

    更新日期:2018-11-01 00:00:00

  • Effects of altering the germination potential of Bacillus anthracis spores by exogenous means in a mouse model.

    abstract::Inhalational anthrax is the most severe form of anthrax. It has been shown in small-animal and non-human primate models that relatively large pools of ungerminated Bacillus anthracis spores can remain within the alveolar spaces for days to weeks post-inhalation or until transported to areas more favourable for germina...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Cote CK,Bozue J,Twenhafel N,Welkos SL

    更新日期:2009-06-01 00:00:00

  • Characteristics of antibiotic-resistant Escherichia coli in the rectum of healthy school-children.

    abstract::During a 3-year period, 771 rectal swabs were taken from abacteriuric school-children. Out of 709 E. coli strains, each isolated from one faecal specimen, 102 were found to be resistant to one or more antibacterial agents, and 607 to be fully sensitive. Another 204 resistant strains were found by selection for antibio...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Lidin-Janson G,Falsen E,Jodal U,Kaijser B,Lincoln K

    更新日期:1977-08-01 00:00:00

  • Immunological characterisation of rubella virion polypeptides.

    abstract::Antibodies specific for rubella virion polypeptide, VPI, secreted by clones of hybridoma cells or produced in rabbits in response to specific antigenic stimulation, located determinants for haemagglutination inhibiting (HI) and neutralising (Nt) antibodies on this envelope component. ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Ho-Terry L,Cohen A,Tedder RS

    更新日期:1984-02-01 00:00:00

  • Involvement of efflux mechanisms in biocide resistance of Campylobacter jejuni and Campylobacter coli.

    abstract::Active efflux has an important role in the antimicrobial resistance of Campylobacter jejuni and Campylobacter coli. The effects of two putative efflux pump inhibitors (EPIs), phenylalanine-arginine β-naphthylamide and 1-(1-naphthylmethyl)-piperazine, and the effects of inactivation of the cmeB,cmeF and cmeR genes on r...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Mavri A,Smole Možina S

    更新日期:2012-06-01 00:00:00

  • Changes in antimicrobial resistance of clinical isolates of Acinetobacter baumannii group isolated in Greece, 2010-2015.

    abstract::In recent years, hospitals in southeastern Europe have faced dramatically high rates of antibiotic-resistant Acinetobacter baumannii. We analysed the evolution of resistance among clinical isolates of A. baumannii group obtained from nine tertiary hospitals throughout Greece over 6 years (2010-2015). Identification an...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Dafopoulou K,Tsakris A,Pournaras S

    更新日期:2018-04-01 00:00:00

  • Experiences in fosfomycin susceptibility testing and resistance mechanism determination in Escherichia coli from urinary tract infections in the UK.

    abstract:PURPOSE:With an increase in the numbers of bacterial isolates resistant to first-line antibiotics, there has been a revival in the use of older drugs including fosfomycin with novel mechanisms of action. We aimed to investigate the prevalence and genotypic nature of fosfomycin resistance in Escherichia coli from urinar...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Cottell JL,Webber MA

    更新日期:2019-02-01 00:00:00

  • Expression of perilipin in human promyelocytic cells in response to Anaplasma phagocytophilum infection results in modified lipid metabolism.

    abstract::The obligate intracellular pathogen Anaplasma phagocytophilum is transmitted by ticks and causes human granulocytic anaplasmosis, tick-borne fever of ruminants, and equine and canine granulocytic anaplasmosis. In a previous study, the perilipin (PLIN) gene was identified as one of the genes differentially expressed in...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Manzano-Roman R,Almazán C,Naranjo V,Bloui EF,Kocan KM,de la Fuente J

    更新日期:2008-02-01 00:00:00

  • Passive immunisation against experimental salmonellosis in mice by orally administered hen egg-yolk antibodies specific for 14-kDa fimbriae of Salmonella enteritidis.

    abstract::Chickens were immunised with a preparation of purified 14-kDa fimbriae of Salmonella serotype Enteritidis (SEF 14) to raise egg-yolk antibodies for protection trials in mice against subsequent challenge-exposure with the homologous strain of Enteritidis. A pronounced specificity of egg-yolk antibodies against the 14-k...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Peralta RC,Yokoyama H,Ikemori Y,Kuroki M,Kodama Y

    更新日期:1994-07-01 00:00:00

  • A new chromosomal locus associated with gut-modulated phenotypes in Salmonella enterica serotype typhimurium.

    abstract::A cosmid DNA library had been constructed previously from 40-kb fragments of genomic DNA from a virulent invasive strain of Salmonella enterica serotype Typhimurium (TML) in an avirulent hypo-invasive Typhimurium strain (LT7). Selection of invasive clones from the library was attempted by iterative passage through a r...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Robey M,Morgan E,Lodge JM,Bolton AJ,Martin GD,Brown NL,Stephen J

    更新日期:2002-03-01 00:00:00

  • Dose escalation studies with caspofungin against Candida glabrata.

    abstract::Echinocandins are recommended as first-line agents against invasive fungal infections caused by Candida glabrata, which still carry a high mortality rate. Dose escalation of echinocandins has been suggested to improve the clinical outcome against C. glabrata. To address this possibility, we performed in vitro and in v...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Domán M,Kovács R,Perlin DS,Kardos G,Gesztelyi R,Juhász B,Bozó A,Majoros L

    更新日期:2015-09-01 00:00:00

  • Carriage of inhibitor-producing organisms on human skin.

    abstract::The carriage on skin lesions, on healthy skin and in the nose, of micro-organisms that produce antibiotics active against Staphylococcus aureus has been studied. About 5% of sites (10% of all patients) yielded such organisms. No evidence could be found that the presence of producer organisms in skin lesions had a prot...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Noble WC,Willie JA

    更新日期:1980-05-01 00:00:00

  • High frequency of cultivable human subgroup F adenoviruses in stool samples from a paediatric population admitted to hospital with acute gastroenteritis.

    abstract::The family Adenoviridae consists of five genera of which the genus Mastadenovirus includes human viruses classified into 57 serotypes clustered into seven subgroups (A-G). Serotypes 40 and 41 (subgroup F) are specifically associated with childhood gastroenteritis and are the most common cause of acute gastroenteritis ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Arcangeletti MC,Germini D,Martorana D,Rodighiero I,De Conto F,Medici MC,Chezzi C,Calderaro A

    更新日期:2014-06-01 00:00:00

  • In-vitro susceptibility of Giardia lamblia to albendazole, mebendazole and other chemotherapeutic agents.

    abstract::The susceptibility of a strain of Giardia lamblia to benzimidazole carbamates, 5-nitroimidazoles, nitrofurans and other drugs was studied in vitro. Albendazole was the most active compound, with a 50% inhibitory concentration (IC50) of 0.01 mg/L and a minimal lethal concentration (MLC) of less than 0.04 mg/L; the IC50...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Cedillo-Rivera R,Muñoz O

    更新日期:1992-09-01 00:00:00

  • Interleukin-8 release from human neutrophils after phagocytosis of Listeria monocytogenes and Yersinia enterocolitica.

    abstract::The release of interleukin-8 (IL-8) from human neutrophils was investigated after their internalisation of the invasive bacteria Listeria monocytogenes and Yersinia enterocolitica. Three pairs of bacterial strains were used to study the influence of listeriolysin O, invasiveness and Yersinia virulence plasmid (pYV)-en...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Arnold R,König W

    更新日期:1998-01-01 00:00:00