Effect of carbapenems on the transcriptional expression of the oprD, oprM and oprN genes in Pseudomonas aeruginosa.

Abstract:

:The effects of imipenem and meropenem on the transcriptional expression of resistance-related genes oprD, oprM and oprN in Pseudomonas aeruginosa were studied by quantitative real-time PCR. Four strains were examined: the type strain PT5 (PAO1), its derivatives M7 and PT149, and a clinical isolate, PaKT3. The derivative M7 is a nalB mutant, overexpressing the MexAB-OprM pump, and the derivative PT149 is a nfxC-type mutant, overexpressing the MexEF-OprN pump while it is down-regulated for the OprD protein. After 18 h incubation in broth, the cultures were divided into three portions. Two were supplemented with antibiotics and the other was left antibiotic-free as the control. After a further 45 min incubation, total RNA was isolated from the strains by guanidine denaturation and acid-phenol/chloroform extraction. DNA-free total RNAs were immediately reverse-transcribed by MMuLV reverse transcriptase. Concentrations of mRNAs obtained by quantitative PCR were expressed relative to uninduced portions of the strains. The results showed that oprD was relatively stable against carbapenem antibiotics. oprM was induced significantly by imipenem in only one strain and oprN was induced by imipenem in most of the strains. The responses at the mRNA level found here were unexpected and suggested a chaotic, unpredictable regulatory mechanism.

journal_name

J Med Microbiol

authors

Kolayli F,Karadenizli A,Savli H,Ergen K,Hatirnaz O,Balikci E,Budak F,Vahaboglu H

doi

10.1099/jmm.0.45692-0

keywords:

subject

Has Abstract

pub_date

2004-09-01 00:00:00

pages

915-920

issue

Pt 9

eissn

0022-2615

issn

1473-5644

journal_volume

53

pub_type

杂志文章
  • Clostridium difficile colonization in healthy adults: transient colonization and correlation with enterococcal colonization.

    abstract::The aim of the present study was to investigate the colonization status of Clostridium difficile in healthy individuals. In total, 139 healthy adults from two study groups were examined at intervals of 3 months. Among the 18 positive subjects, the number of subjects from whom C. difficile was isolated once, twice, thr...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.05376-0

    authors: Ozaki E,Kato H,Kita H,Karasawa T,Maegawa T,Koino Y,Matsumoto K,Takada T,Nomoto K,Tanaka R,Nakamura S

    更新日期:2004-02-01 00:00:00

  • Rapid molecular differentiation and genotypic heterogeneity among Candida parapsilosis and Candida orthopsilosis strains isolated from clinical specimens in Kuwait.

    abstract::Recent molecular studies have led to the recognition of three distinct species within the Candida parapsilosis complex, namely Candida parapsilosis, Candida orthopsilosis and Candida metapsilosis. As currently available yeast identification systems fail to differentiate these species, there is a paucity of information...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.008235-0

    authors: Asadzadeh M,Ahmad S,Al-Sweih N,Khan ZU

    更新日期:2009-06-01 00:00:00

  • The effect of nebulised pentamidine on the concentration of intra-oral Candida albicans in HIV-infected patients.

    abstract::This prospective study investigated whether oral candidal carriage in HIV-infected patients was altered by pentamidine therapy. Repeated oral rinses were taken from 56 HIV-positive patients over a 2-year period. Oral candidal carriage was investigated in two groups of patients, one receiving prophylactic pentamidine t...

    journal_title:Journal of medical microbiology

    pub_type: 临床试验,杂志文章

    doi:10.1099/00222615-41-2-95

    authors: Nolan A,Lamey PJ,MacFarlane TW,Aitchison TC,Shaw J,Sirel JY

    更新日期:1994-08-01 00:00:00

  • Bacteroides fragilis is a silent pathogen in acute appendicitis.

    abstract::The role of Bacteroides fragilis in the pathogenesis of acute appendicitis was studied in 135 patients in four patient groups: normal (17); phlegmonous appendicitis (17); gangrenous appendicitis (75); and septic complications of appendicitis (26). Aerobic and anaerobic bacteria were isolated from all groups and member...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-21-3-245

    authors: Elhag KM,Alwan MH,Al-Adnani MS,Sherif RA

    更新日期:1986-05-01 00:00:00

  • Evaluation of the polymerase chain reaction for detecting the urease C gene of Helicobacter pylori in gastric biopsy samples and dental plaque.

    abstract::A polymerase chain reaction (PCR) assay with oligonucleotide primers homologous to a portion of the urease C gene of Helicobacter pylori was evaluated for specificity with pure DNA and biopsy material. The assay was used to test for the presence of the organism in dental plaque. The species specificity of detection wa...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-39-5-338

    authors: Bickley J,Owen RJ,Fraser AG,Pounder RE

    更新日期:1993-11-01 00:00:00

  • Identifying indoor air Penicillium species: a challenge for allergic patients.

    abstract:PURPOSE:Penicillium is the most common mould isolated in housing. Penicillium chrysogenum is the only species tested by prick test or serology for allergic patients. The American Institute of Medicine has accepted Penicillium as an aetiological agent of rhinitis in children and adults and as an asthma agent in children...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000960

    authors: Reboux G,Rocchi S,Vacheyrou M,Millon L

    更新日期:2019-05-01 00:00:00

  • The bactericidal activity of DR-3355, an optically active isomer of ofloxacin.

    abstract::The bactericidal activity of compound DR-3355, an optically active isomer of ofloxacin, was measured against Escherichia coli, Staphylococcus aureus and S. epidermidis, in nutrient broth and in phosphate-buffered saline. DR-3355 was found to be approximately twice as active as ofloxacin in terms of the concentration a...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-30-3-227

    authors: Lewin CS,Amyes SG

    更新日期:1989-11-01 00:00:00

  • Susceptibility of oral bacterial biofilms to antimicrobial agents.

    abstract::There is great interest in the use of antimicrobial agents for the prevention and treatment of plaque-related oral diseases and many publications have reported the results of studies in which the minimum inhibitory concentrations of agents for cariogenic and periodontopathogenic bacteria have been determined. However,...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章,评审

    doi:10.1099/00222615-44-2-79

    authors: Wilson M

    更新日期:1996-02-01 00:00:00

  • High prevalence of multidrug-resistant Escherichia coli isolates from children with and without diarrhoea and their susceptibility to the antibacterial activity of extracts/fractions of fruits native to Mexico.

    abstract:PURPOSE:This paper aims to evaluate the antimicrobial resistance of Esherichia coli isolates from children under 5 years old, with and without diarrhoea, who were hospital outpatients in Culiacan, Sinaloa, Mexico. It also looks at the antimicrobial activity of fruit extracts against selected multidrug-resistant (MDR) E...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000548

    authors: Uribe-Beltrán MJ,Ahumada-Santos YP,Díaz-Camacho SP,Eslava-Campos CA,Reyes-Valenzuela JE,Báez-Flores ME,Osuna-Ramírez I,Delgado-Vargas F

    更新日期:2017-07-01 00:00:00

  • Mechanism of persistent infection associated with peritoneal implants.

    abstract::The ability of rabbits to clear an intraperitoneal injection of Pseudomonas aeruginosa in the presence or absence of a surgically implanted peritoneal device was investigated. Sham-operated rabbits without an implant eliminated a P. aeruginosa challenge of 5 x 10(6) cfu/ml; lavage fluid and peritoneal tissues became c...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-36-6-406

    authors: Ward KH,Olson ME,Lam K,Costerton JW

    更新日期:1992-06-01 00:00:00

  • RNA-dependent DNA-polymerase activity in human milk.

    abstract::A simple method is described for testing milk specimens from nursing mothers for the presence of RNA-dependent DNA-polymerase activity. Positive results were obtained in five of 137 women (3.6%) without a family history of breast cancer, and in six of 31 women (19.3%) with a family history of breast cancer. ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-8-3-447

    authors: McFarlane ES,Ryan SM,Mann E

    更新日期:1975-08-01 00:00:00

  • Identification by PCR of Helicobacter pylori in subgingival plaque of adult periodontitis patients.

    abstract::The PCR was used to detect the presence of Helicobacter pylori in subgingival plaque samples from patients with adult periodontitis. Primers based upon the 16S ribosomal RNA (rRNA) gene sequence of H. pylori were used in a single round of PCR to amplify a 295-bp DNA fragment and the identity of the amplified products ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-48-3-317

    authors: Riggio MP,Lennon A

    更新日期:1999-03-01 00:00:00

  • Coccidioidal pericarditis: a rapid presumptive diagnosis by an in-house antigen confirmed by mycological and molecular methods.

    abstract::Coccidioidal pericarditis is a condition found in approximately 1-5% of patients infected by Coccidioides species. It is associated with widely diverse clinical symptoms. This paper reports a case of coccidioidal pericarditis diagnosed by an in-house Coccidioides posadasii antigen and confirmed with mycological and mo...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.2008/002428-0

    authors: Brilhante RSN,Cordeiro RA,Rocha MFG,Fechine MAB,Furtado FM,Nagao-Dias AT,Camargo ZP,Sidrim JJC

    更新日期:2008-10-01 00:00:00

  • Balamuthia mandrillaris resistance to hostile conditions.

    abstract::The resistance of Balamuthia mandrillaris to physical, chemical and radiological conditions was tested. Following treatments, viability was determined by culturing amoebae on human brain microvascular endothelial cells for up to 12 days. B. mandrillaris cysts were resistant to repeated freeze-thawing (five times), tem...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.47694-0

    authors: Siddiqui R,Ortega-Rivas A,Khan NA

    更新日期:2008-04-01 00:00:00

  • The relationship between opacity factor and M protein in Streptococcus pyogenes.

    abstract::Lancefield acid extracts of Streptococcus pyogenes, type 22 (T12, M22, OF positive) gave good yields of M protein and little opacity factor (OF), but sodium dodecyl sulphate (SDS) extracts contained high titres of OF (greater than 20000) and little M protein. Acid-extracted OF could be separated from M protein by Seph...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-16-1-13

    authors: Hallas G,Widdowson JP

    更新日期:1983-02-01 00:00:00

  • Characterization of the clonal subpopulation Fiocruz L1-130 of Leptospira interrogans in rats and dogs from Brazil.

    abstract:PURPOSE:Leptospira interrogans serogroup Icterohaemorrhagiae strains have been described as causing disease in both humans and animals and as being present worldwide. Icterohaemorrhagiae and Copenhageni serovars are known to cause severe disease in their hosts, and zoonotic outbreaks have been described. The genetic si...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000806

    authors: Jaeger LH,Pestana CP,Carvalho-Costa FA,Medeiros MA,Lilenbaum W

    更新日期:2018-09-01 00:00:00

  • In-vitro inhibition of mycobacteria by viridans streptococci.

    abstract::Eleven strains of Mycobacterium tuberculosis and 13 other species of mycobacteria were examined for susceptibility to 26 strains of viridans streptococci. Susceptibility was detected by the simultaneous antagonism technique, but not by tests of deferred antagonism. It is concluded that in-vitro inhibition of mycobacte...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-19-2-227

    authors: Allen BW

    更新日期:1985-04-01 00:00:00

  • Isolation and characterisation of neurotoxigenic Clostridium butyricum from soil in China.

    abstract::Soil specimens collected from a site around the home of patients with food-borne type E. botulism probably caused by neurotoxigenic Clostridium butyricum in Guanyun, Jiangsu province, China, were examined for the presence of neurotoxigenic C. butyricum. Five lakeside sites of Weishan lake, in an area near to the sites...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-48-2-133

    authors: Meng X,Yamakawa K,Zou K,Wang X,Kuang X,Lu C,Wang C,Karasawa T,Nakamura S

    更新日期:1999-02-01 00:00:00

  • Frequency of four different mutacin genes in Streptococcus mutans genotypes isolated from caries-free and caries-active individuals.

    abstract::The ability of Streptococcus mutans to produce mutacins, combined with the production of other virulence factors such as lactic acid, may contribute to the pathogenesis of this bacterium. In the present study, the detection of genes encoding mutacin types I/III, II and IV was performed by PCR with specific primers to ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.45870-0

    authors: Kamiya RU,Napimoga MH,Höfling JF,Gonçalves RB

    更新日期:2005-06-01 00:00:00

  • Enhanced active efflux, repression of porin synthesis and development of Mar phenotype by diazepam in two enterobacteria strains.

    abstract::The aim of this work was to determine whether diazepam could induce the multiple antibiotic resistance (Mar) phenotype in Klebsiella pneumoniae and Escherichia coli strains. The Mar phenotype is characterized by decreased susceptibility to multiple antibiotics due to the loss of porins and/or increased expression of a...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.45613-0

    authors: Tavío MM,Vila J,Perilli M,Casañas LT,Maciá L,Amicosante G,Jiménez de Anta MT

    更新日期:2004-11-01 00:00:00

  • Changes in genetic types and population dynamics of Moraxella catarrhalis in hospitalized children are not associated with an exacerbation of existing disease.

    abstract::Pulsed-field gel electrophoresis typing was performed on a retrospective set of 129 Moraxella catarrhalis isolates obtained over a 20 month period from 70 children admitted to, or presenting at, the Erasmus University Medical Center, Rotterdam, The Netherlands. The mean age of the children (at the end of the study) wa...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.05251-0

    authors: Hays JP,Eadie K,Verduin CM,Hazelzet J,Verbrugh H,van Belkum A

    更新日期:2003-09-01 00:00:00

  • Evaluation of internal transcribed spacer 2-RFLP analysis for the identification of dermatophytes.

    abstract::A total of 95 isolates, belonging to 33 species of five dermatophyte genera, i.e. Arthroderma (15 species), Chrysosporium (two), Epidermophyton (one), Microsporum (three) and Trichophyton (12), were studied using internal transcribed spacer 2 (ITS2)-PCR-RFLP analysis (ITS2-RFLP), consisting of amplification of the ITS...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.013870-0

    authors: De Baere T,Summerbell R,Theelen B,Boekhout T,Vaneechoutte M

    更新日期:2010-01-01 00:00:00

  • Purification and characterisation of a metallopeptidase of Candida albicans.

    abstract::A novel aminopeptidase was purified by high performance liquid chromatography from a cytosoluble 100,000 g extract of Candida albicans on the basis of its ability to cleave L-arginine 7-amino-4-methylcoumarin. The purification factor was 36 and the yield was 20%. The native enzyme had a mol. wt of 52 kDa as demonstrat...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-43-4-282

    authors: el Moudni B,Rodier MH,Barrault C,Ghazali M,Jacquemin JL

    更新日期:1995-10-01 00:00:00

  • Continuous culture of sessile human oropharyngeal microbiotas.

    abstract::The microbiota of the human oropharynx plays an important role in health through involvement in the aetiology of infection and the carriage of adventitious pathogens. Despite this, there are few models available for the preclinical assessment of novel antimicrobials directed to the human throat. We have profiled bacte...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.055806-0

    authors: Humphreys GJ,McBain AJ

    更新日期:2013-06-01 00:00:00

  • Analysis of methods commonly used for glycopeptide and oxazolidinone susceptibility testing in Enterococcus faecium isolates.

    abstract::The susceptibility to teicoplanin, vancomycin and linezolid of 30 clinical isolates of Enterococcus faecium was tested by Vitek 2, Phoenix, Etest, broth microdilution and disc diffusion tests. The vanA and vanB resistance genes and the 23S rRNA gene G2576T mutation were detected by PCR and PCR-RFLP, respectively. Resi...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.016444-0

    authors: Raponi G,Ghezzi MC,Gherardi G,Lorino G,Dicuonzo G

    更新日期:2010-06-01 00:00:00

  • Production of extracellular matrix by Candida albicans biofilms.

    abstract::Growth of Candida albicans biofilms and production of extracellular matrix were monitored by dry weight, colorimetric and radioisotope assays, and by scanning electron microscopy. Under static incubation conditions synthesis of matrix material was minimal, but increased dramatically when developing biofilms were subje...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-47-3-253

    authors: Hawser SP,Baillie GS,Douglas LJ

    更新日期:1998-03-01 00:00:00

  • Multilocus genetic analysis reveals that the Australian strains of Vibrio cholerae O1 are similar to the pre-seventh pandemic strains of the El Tor biotype.

    abstract::Episodes of cholera stemming from indigenous Vibrio cholerae strains in Australia are mainly associated with environmental sources. In the present study, 10 V. cholerae O1 strains of Australian origin were characterized. All of the strains were serogroup O1 and their conventional phenotypic traits categorized them as ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.004333-0

    authors: Safa A,Bhuiyan NA,Murphy D,Bates J,Nusrin S,Kong RYC,Chongsanguan M,Chaicumpa W,Nair GB

    更新日期:2009-01-01 00:00:00

  • Identification and comparative analysis of the lactoferrin and transferrin receptors among clinical isolates of gonococci.

    abstract::Neisseria gonorrhoeae expresses receptors for both lactoferrin (LF) and transferrin (TF). To determine whether qualitative or quantitative changes in these receptors, or both, correlate with the pattern of disease due to N. gonorrhoeae, a dot binding assay with whole cells was used to measure the absolute receptor lev...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-28-3-199

    authors: Lee BC,Bryan LE

    更新日期:1989-03-01 00:00:00

  • Isolation and characterisation of a novel spirochaete from severe virulent ovine foot rot.

    abstract::A novel spirochaete was isolated from a case of severe virulent ovine foot rot (SVOFR) by immunomagnetic separation with beads coated with polyclonal anti-treponemal antisera and prolonged anaerobic broth culture. The as yet unnamed treponeme differs considerably from the only other spirochaete isolated from ovine foo...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-50-12-1061

    authors: Demirkan I,Carter SD,Winstanley C,Bruce KD,McNAIR NM,Woodside M,Hart CA

    更新日期:2001-12-01 00:00:00

  • Analysis of the porphyrin content of fluorescent pus by absorption spectrophotometry and high performance liquid chromatography.

    abstract::Extracts of 19 samples of pus which showed red fluorescence with ultraviolet light were screened for the presence of porphyrins by absorption spectrophotometry. All those which showed spectra typical of metal-free porphyrins were analysed by high performance liquid chromatography to identify the porphyrins present. Th...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-33-1-29

    authors: Brazier JS

    更新日期:1990-09-01 00:00:00