Clostridium difficile colonization in healthy adults: transient colonization and correlation with enterococcal colonization.

Abstract:

:The aim of the present study was to investigate the colonization status of Clostridium difficile in healthy individuals. In total, 139 healthy adults from two study groups were examined at intervals of 3 months. Among the 18 positive subjects, the number of subjects from whom C. difficile was isolated once, twice, three times or four times was 10 (55.6%), three (16.7%), two (11.1%) and three (16.7%), respectively. In the student group, different subjects were colonized by different PCR ribotype/PFGE types. However, the same PCR ribotype/PFGE types of C. difficile were isolated from different subjects in the employee group, indicating that cross-transmission may have occurred in this group. Continuous colonization by the same PCR ribotype/PFGE type was only observed in three subjects. C. difficile-positive subjects were significantly more densely colonized by enterococci (P<0.05) than C. difficile-negative subjects: subjects that were found to be C. difficile-positive three or four times appeared to have higher concentrations of enterococci. The present results demonstrate that, although colonization by a C. difficile strain is transient in many cases, there are healthy individuals that are colonized persistently by C. difficile. They also suggest that dense colonization of the intestine by enterococci may be associated with C. difficile colonization.

journal_name

J Med Microbiol

authors

Ozaki E,Kato H,Kita H,Karasawa T,Maegawa T,Koino Y,Matsumoto K,Takada T,Nomoto K,Tanaka R,Nakamura S

doi

10.1099/jmm.0.05376-0

keywords:

subject

Has Abstract

pub_date

2004-02-01 00:00:00

pages

167-172

issue

Pt 2

eissn

0022-2615

issn

1473-5644

journal_volume

53

pub_type

杂志文章
  • Airborne transmission of lyssaviruses.

    abstract::In 2002, a Scottish bat conservationist developed a rabies-like disease and subsequently died. This was caused by infection with European bat lyssavirus 2 (EBLV-2), a virus closely related to Rabies virus (RABV). The source of this infection and the means of transmission have not yet been confirmed. In this study, the...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.46370-0

    authors: Johnson N,Phillpotts R,Fooks AR

    更新日期:2006-06-01 00:00:00

  • A purulent pericarditis caused by Salmonella typhimurium.

    abstract::A case of Salmonella typhimurium pericarditis is reported. The diagnosis was based on blood and pericardial effusion cultures. ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.05449-0

    authors: Can F,Demirbilek M,Erdem B,Ciftci U,Tunaoglu M,Laleli Y

    更新日期:2004-10-01 00:00:00

  • Evaluation of a newly developed down-flow immunoassay for detection of serum mannan antigens in patients with candidaemia.

    abstract::A down-flow immunoassay has been developed to detect serum mannan antigens, and the test was recently marketed as the Unimedi Candida monotest. Using 251 serum samples from 105 patients with candidaemia, a comparison of the Unimedi Candida monotest with the Cand-Tec latex agglutination test and 2 microplate enzyme imm...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.46314-0

    authors: Fujita SI,Takamura T,Nagahara M,Hashimoto T

    更新日期:2006-05-01 00:00:00

  • The effect of human serum DNAases on the ability to detect antibiotic-killed Escherichia coli in blood by PCR.

    abstract::PCR has proved superior to conventional blood culture for diagnosing bacteraemia in the presence of antibiotics. Nevertheless, even PCR might yield false-negative results if the template DNA were to be cleaved by serum DNAases after antibiotics had induced bacterial death. To evaluate the cleavage of bacterial templat...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-50-3-243

    authors: Heininger A,Ulrich M,Priebe G,Unertl K,Müller-Schauenburg A,Botzenhart K,Döring G

    更新日期:2001-03-01 00:00:00

  • Comparative analysis of phenol-soluble modulin production and Galleria mellonella killing by community-associated and healthcare-associated meticillin-resistant Staphylococcusaureus strains.

    abstract::Community-associated meticillin-resistant Staphylococcus aureus (CA-MRSA) have emerged globally and have been associated with more severe disease than healthcare-associated MRSA (HA-MRSA). The purpose of this study was to determine whether laboratory measures of virulence can distinguish dominant CA-MRSA clones from H...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000379

    authors: Boakes E,Marbach H,Lynham S,Ward M,Edgeworth JD,Otter JA

    更新日期:2016-12-01 00:00:00

  • Molecular analysis and species diversity of Nocardia in the hospital environment in a developing country, a potential health hazard.

    abstract:PURPOSE:Despite hundreds of reports on the isolation of Nocardia from clinical samples, the presence and diversity of Nocardia species that are capable of survival in a harsh and adverse condition, such as a hospital environment, have not been comprehensively studied. The aim of this study was to assess Nocardia specie...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000436

    authors: Rahdar HA,Azadi D,Shojaei H,Daei-Naser A

    更新日期:2017-03-01 00:00:00

  • Laboratory challenges in the diagnosis of hepatitis E virus.

    abstract::Hepatitis E virus (HEV) is an RNA virus that is an important cause of both acute and chronic hepatitis worldwide. To date, there are eight HEV genotypes that can infect mammals. HEV-1 and HEV-2 infect exclusively humans, while HEV-3 and HEV-4 infect humans and various animals, mainly pigs and deer. Additionally, two n...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章,评审

    doi:10.1099/jmm.0.000706

    authors: Al-Sadeq DW,Majdalawieh AF,Mesleh AG,Abdalla OM,Nasrallah GK

    更新日期:2018-04-01 00:00:00

  • Bacteroides fragilis is a silent pathogen in acute appendicitis.

    abstract::The role of Bacteroides fragilis in the pathogenesis of acute appendicitis was studied in 135 patients in four patient groups: normal (17); phlegmonous appendicitis (17); gangrenous appendicitis (75); and septic complications of appendicitis (26). Aerobic and anaerobic bacteria were isolated from all groups and member...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-21-3-245

    authors: Elhag KM,Alwan MH,Al-Adnani MS,Sherif RA

    更新日期:1986-05-01 00:00:00

  • Susceptibilities of Actinobacillus actinomycetemcomitans biofilms to oral antiseptics.

    abstract::The susceptibilities of Actinobacillus actinomycetemcomitans cultures, grown as 1- or 3-day-old biofilms or as planktonic suspensions, to concentrations of chlorhexidine digluconate, cetylpyridinium chloride or triclosan used in commercial mouthwashes were compared. Three-day biofilms were the most resistant form of t...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-46-5-425

    authors: Thrower Y,Pinney RJ,Wilson M

    更新日期:1997-05-01 00:00:00

  • Bacterial adhesion and biofilm formation on yttria-stabilized, tetragonal zirconia and titanium oral implant materials with low surface roughness - an in situ study.

    abstract::Bacterially-driven mucosal inflammation and the development of periimplantitis can lead to oral implant failure. In this study, initial bacterial adhesion after 2 h, and biofilm formation after 1 day and 3 days, were analysed in situ on novel 3 mol % yttria-stabilized tetragonal zirconia polycrystal samples, as well a...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000267

    authors: Al-Ahmad A,Karygianni L,Schulze Wartenhorst M,Bächle M,Hellwig E,Follo M,Vach K,Han JS

    更新日期:2016-07-01 00:00:00

  • In vitro activities of vancomycin and linezolid against biofilm-producing methicillin-resistant staphylococci species isolated from catheter-related bloodstream infections from an Egyptian tertiary hospital.

    abstract:PURPOSE:Catheter-related bloodstream infections (CRBSIs) are among the most common hospital-acquired infections. We aimed to survey methicillin resistance, biofilm production and susceptibility to vancomycin, linezolid and other antibiotics for staphylococci isolated from CRBSIs. METHODOLOGY:Fifty-eight isolates [20 S...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000490

    authors: Hashem AA,Abd El Fadeal NM,Shehata AS

    更新日期:2017-06-01 00:00:00

  • The routine application of a microtechnique for the demonstration of leptospiral antibodies.

    abstract::The microtechnique of Galton et al. (1965) for the routine screening of blood samples for leptospiral antibodies was used to examine more than 8000 serum samples from animals that had aborted and from herds in which leptospiral antibodies had previously been demonstrated. The method gave consistently accurate results....

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-11-3-355

    authors: Lewis J

    更新日期:1978-08-01 00:00:00

  • Chemotactic response of Helicobacter pylori to human plasma and bile.

    abstract::To clarify further the role of chemotaxis in Helicobacter pylori colonization, the in vitro bacterium response to human plasma and bile (secretions containing chemoeffector compounds that are present in the gastric mucus layer) was examined. Human plasma, after dilution to 1 % (v/v) with buffer, was found to be a chem...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.45636-0

    authors: Worku ML,Karim QN,Spencer J,Sidebotham RL

    更新日期:2004-08-01 00:00:00

  • Mechanism of persistent infection associated with peritoneal implants.

    abstract::The ability of rabbits to clear an intraperitoneal injection of Pseudomonas aeruginosa in the presence or absence of a surgically implanted peritoneal device was investigated. Sham-operated rabbits without an implant eliminated a P. aeruginosa challenge of 5 x 10(6) cfu/ml; lavage fluid and peritoneal tissues became c...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-36-6-406

    authors: Ward KH,Olson ME,Lam K,Costerton JW

    更新日期:1992-06-01 00:00:00

  • Comparison of conventional culture with SeptiFast real-time PCR for microbial pathogen detection in clinical specimens other than blood.

    abstract::Early detection of aetiological agents is pivotal for adequate therapy for bacterial infections. Although culture is still considered the mainstay for laboratory diagnosis, it often lacks sensitivity, especially in patients already treated with antibiotics. The present study investigated the potential clinical utility...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.034280-0

    authors: Mencacci A,Leli C,Cardaccia A,Montagna P,Moretti A,Bietolini C,Meucci M,Perito S,Cenci E,Bistoni F

    更新日期:2011-12-01 00:00:00

  • Microbial profiling of dental plaque from mechanically ventilated patients.

    abstract::Micro-organisms isolated from the oral cavity may translocate to the lower airways during mechanical ventilation (MV) leading to ventilator-associated pneumonia (VAP). Changes within the dental plaque microbiome during MV have been documented previously, primarily using culture-based techniques. The aim of this study ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000212

    authors: Sands KM,Twigg JA,Lewis MAO,Wise MP,Marchesi JR,Smith A,Wilson MJ,Williams DW

    更新日期:2016-02-01 00:00:00

  • In vitro assessment of the APTIMA Combo 2 assay for the detection of Chlamydia trachomatis using highly purified elementary bodies.

    abstract::The Gen-Probe APTIMA Combo 2 assay has previously been reported to have a high sensitivity. The end point of this assay was evaluated using highly purified chlamydial elementary bodies (EBs). The performance of the APTIMA Combo 2 assay was compared with a commercially available PCR kit, AMPLICOR Chlamydia trachomatis....

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.45911-0

    authors: Ikeda-Dantsuji Y,Konomi I,Nagayama A

    更新日期:2005-04-01 00:00:00

  • Characterisation of the external surfaces of Pseudomonas aeruginosa isolated from human blood and respiratory tract.

    abstract::The surface structures of the cell envelopes of 16 clinical isolates of Pseudomonas aeruginosa were examined by electronmicroscopy with the new fixation technique of freeze-substitution. Two types of structures were observed among the organisms. In one group of strains, mostly isolated from blood, a dense fibrous laye...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-40-4-282

    authors: Misumi H,Umeda A,Eguchi K,Okada K,Sawae Y,Niho Y,Amako K

    更新日期:1994-04-01 00:00:00

  • Ovine systemic pasteurellosis caused by Pasteurella haemolytica biotype T.

    abstract::A detailed study was made of lambs aged 5--7 months naturally infected with Pasteurella haemolytica biotype T. In addition to the well known features of such infections, previously unreported necrotic lesions of the tonsil, oesophagus, pharynx and adjacent areas were consistently seen. Large numbers of P. haemolytica ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-14-1-89

    authors: Dyson DA,Gilmour NJ,Angus KW

    更新日期:1981-02-01 00:00:00

  • Restoring the activity of the antibiotic aztreonam using the polyphenol epigallocatechin gallate (EGCG) against multidrug-resistant clinical isolates of Pseudomonas aeruginosa.

    abstract::Introduction. Pseudomonas aeruginosa is an important Gram-negative pathogen that is intrinsically multidrug-resistant (MDR) and frequently associated with healthcare-associated outbreaks. With increasing resistance to antibiotics and with very few novel drugs under development, clinicians often use combinations to tre...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.001060

    authors: Betts JW,Hornsey M,Higgins PG,Lucassen K,Wille J,Salguero FJ,Seifert H,La Ragione RM

    更新日期:2019-10-01 00:00:00

  • Apoptosis-like death in Leishmania donovani promastigotes induced by eugenol-rich oil of Syzygium aromaticum.

    abstract::Leishmaniasis consists of a complex spectrum of infectious diseases with worldwide distribution of which visceral leishmaniasis or kala-azar caused by Leishmania donovani is the most devastating. In the absence of vaccines, chemotherapy remains the mainstay for the control of leishmaniasis. The drugs of choice are exp...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.064709-0

    authors: Islamuddin M,Sahal D,Afrin F

    更新日期:2014-01-01 00:00:00

  • The use of bacteriophages to differentiate serologically cross-reactive isolates of Klebsiella pneumoniae.

    abstract::In serological typing of Klebsiella pneumoniae strains from human, equine and environmental sources, the capsular identity of many isolates could not be determined because of serological cross-reactivity. A panel of 91 bacteriophages able to lyse each of the 77 capsular serotypes of K. pneumoniae was isolated and test...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-41-6-423

    authors: Pieroni P,Rennie RP,Ziola B,Deneer HG

    更新日期:1994-12-01 00:00:00

  • Experimental infection of Wistar rats with 'Gastrospirillum suis'.

    abstract::In order to develop a model for the study of gastric spiral bacteria, and based on the observation that Wistar rats do not carry urease-positive spiral bacteria in their gastric mucosa, mucus from a pig naturally colonised by 'Gastrospirillum suis' (an organism with I6S rDNA 99.5% similar to that of 'G. hominis' type ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-44-2-105

    authors: Mendes EN,Queiroz DM,Coimbra RS,Moura SB,Barbosa AJ,Rocha GA

    更新日期:1996-02-01 00:00:00

  • Effect of EDTA on the resistance of clinical isolates of Acinetobacter baumannii to the bactericidal activity of normal human serum.

    abstract::Acinetobacter baumannii is an opportunist nosocomial pathogen of world-wide importance and produces severe infections in immunocompromised patients. However, the virulence factors contributing to its pathogenic properties are not well known. The effect of normal human serum against 18 clinical isolates of the most pre...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-49-11-1047

    authors: García A,Solar H,González C,Zemelman R

    更新日期:2000-11-01 00:00:00

  • Application of terminal RFLP analysis to characterize oral bacterial flora in saliva of healthy subjects and patients with periodontitis.

    abstract::Terminal restriction fragment-length polymorphism (T-RFLP) analysis was applied to characterize oral bacterial flora in saliva from 18 healthy subjects and 18 patients with periodontitis. The 16S rRNA genes (rDNAs) of oral bacteria and spirochaetes in saliva were amplified by PCR with a 6'carboxy-fluorescein (6-FAM)-l...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.04991-0

    authors: Sakamoto M,Takeuchi Y,Umeda M,Ishikawa I,Benno Y

    更新日期:2003-01-01 00:00:00

  • In vitro efficacy of sodium selenite in reducing toxin production, spore outgrowth and antibiotic resistance in hypervirulent Clostridium difficile.

    abstract:PURPOSE:This study investigated the efficacy of the essential mineral, selenium (sodium selenite), in reducing the toxin production, spore outgrowth and antibiotic resistance of Clostridium difficile in vitro. METHODOLOGY:Two hypervirulent C. difficile isolates were cultured in brain heart infusion broth with and with...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.001008

    authors: Pellissery AJ,Vinayamohan PG,Yin HB,Mooyottu S,Venkitanarayanan K

    更新日期:2019-07-01 00:00:00

  • Evaluation and readout optimization of the rapid polymyxin NP test for the detection of colistin-resistant Enterobacteriaceae.

    abstract:PURPOSE:In an increasing number of cases the last therapeutic option for treatment of carbapenem-resistant Enterobacteriaceae is colistin. As the detection of colistin resistance is problematic and time-consuming, it is desirable to find a rapid and reliable test. The rapid polymyxin NP test developed by Nordmann et al...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.001030

    authors: Belda-Orlowski A,Pfennigwerth N,Gatermann SG,Korte-Berwanger M

    更新日期:2019-08-01 00:00:00

  • A collaborative evaluation of a rapid, semi-automated identification system for gram-negative bacilli: the Quantum II BID.

    abstract::The recently-introduced semi-automated bacterial identification system, the Quantum II BID, is designed to identify fermentative and non-fermentative gram-negative bacilli in 4-5 h. The system was evaluated independently by the two participating laboratories. Inter-laboratory reproducibility was determined by testing ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-21-2-145

    authors: Stevens M,Henrichsen C,Smith M

    更新日期:1986-03-01 00:00:00

  • Analysis of mutations in the gyrA and gyrB genes and their association with the resistance of Mycobacterium tuberculosis to levofloxacin, moxifloxacin and gatifloxacin.

    abstract::The purpose of the present study was to analyse mutations in the gyrA and gyrB genes of Mycobacterium tuberculosis and define the possible correlation between these mutations and resistance to levofloxacin (LVX), moxifloxacin (MFX) and gatifloxacin (GAT), based on their MICs. One hundred and forty-two M. tuberculosis ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.046821-0

    authors: Nosova EY,Bukatina AA,Isaeva YD,Makarova MV,Galkina KY,Moroz AM

    更新日期:2013-01-01 00:00:00

  • Comparative characterisation of the biofilm-production abilities of Staphylococcus epidermidis isolated from human skin and platelet concentrates.

    abstract:PURPOSE:Staphylococcus epidermidis is the predominant contaminant of platelet concentrates (PCs), a blood product used to treat patients with platelet deficiencies. This microorganism is able to form surface-attached aggregates (biofilms) in human skin. Herein, the abundance of S. epidermidis biofilm-producers in conta...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000673

    authors: Taha M,Kohnen C,Mallya S,Kou Y,Zapata A,Ramirez-Arcos S

    更新日期:2018-02-01 00:00:00