Abstract：
:The recently-introduced semi-automated bacterial identification system, the Quantum II BID, is designed to identify fermentative and non-fermentative gram-negative bacilli in 4-5 h. The system was evaluated independently by the two participating laboratories. Inter-laboratory reproducibility was determined by testing 181 strains in each laboratory and found to be 97.8%. A further 893 organisms, comprising 417 fresh clinical isolates and 476 stock cultures, were then tested by the system in the two laboratories. Of the fresh clinical isolates, 95.7% yielded the same result as the comparative system used (API 20E) and 93.1% of the stock strains were assigned to their expected, previously established identity. Overall agreement between the two systems for all strains examined was 94.3%. There was no significant statistical difference between the results obtained in the two laboratories.
journal_name
J Med Microbioljournal_title
Journal of medical microbiologyauthors
Stevens M,Henrichsen C,Smith Mdoi
10.1099/00222615-21-2-145subject
Has Abstractpub_date
1986-03-01 00:00:00pages
145-50issue
2eissn
0022-2615issn
1473-5644journal_volume
21pub_type
杂志文章abstract：:Sixty-three Staphylococcus aureus strains (40 from clinical sources and 23 from food sources) were examined for toxic shock syndrome toxin-1 (TSST-1) using PCR, phage typed using the international phage set (IPS) and tested for their susceptibility to antibiotics. Only three strains (all from clinical sources) were po...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.46162-0
更新日期：2006-02-01 00:00:00
abstract：:Clostridium difficile is a frequent cause of severe, recurrent, post-antibiotic diarrhoea and pseudomembranous colitis. Its pathogenicity is mediated mainly by two toxins, TcdA and TcdB. However, different adhesins have also been described as important colonization factors which are implicated in the first step of the...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.029553-0
更新日期：2011-08-01 00:00:00
abstract：:Resistance to gentamicin, tobramycin and kanamycin (GmrTmrKmr) in strains of Staphylococcus aureus isolated from clinical sources in Australia is mediated by a 4.7 kb transposable element, designated Tn4001. A 2.5 kb HindIII fragment which maps symmetrically within Tn4001, and encompasses the aminoglycoside-resistance...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-23-2-101
更新日期：1987-03-01 00:00:00
abstract：:In January 2015, Public Health England and the United Kingdom (UK) Ministry of Defence investigated cases of diarrhoea and fever in military personnel recently returned to the UK after supporting the response to the Ebola epidemic in Sierra Leone. Tests for Ebola virus infection were negative. PCR tests detected the i...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.000832
更新日期：2018-11-01 00:00:00
abstract：:The role of Bacteroides fragilis in the pathogenesis of acute appendicitis was studied in 135 patients in four patient groups: normal (17); phlegmonous appendicitis (17); gangrenous appendicitis (75); and septic complications of appendicitis (26). Aerobic and anaerobic bacteria were isolated from all groups and member...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-21-3-245
更新日期：1986-05-01 00:00:00
abstract：:A slide agglutination technique identifying 10 serogroups of Clostridium difficile (A,B,C,D,F,G,H,I,K and X) has been described previously. In this study, we have used the hamster to compare the ability of the 10 serogroup reference strains to colonise and produce disease. Groups of four hamsters were each given a sin...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-33-2-85
更新日期：1990-10-01 00:00:00
abstract：:Erysipelothrix rhusiopathiae is a zoonotic pathogen that causes erysipelas in a variety of animals. In humans, in contrast to the cutaneous form called erysipeloid, which is an occupational disease and common in individuals who handle raw meat and fish, invasive systemic infections are unusual. E. rhusiopathiae expres...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.001252
更新日期：2020-10-01 00:00:00
abstract：:One hundred and twenty consecutive isolates of Neisseria gonorrhoeae from the County of Avon, UK, were examined by auxotyping and penicillin susceptibility testing. Requirements for proline (Pro), arginine (Arg), hypoxanthine (Hyp), uracil (Ura) and methionine (Met) were determined with a modified Heckels' medium. Pro...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-16-3-295
更新日期：1983-08-01 00:00:00
abstract：:The adhesion of bovine and a human isolate of Escherichia coli to epithelial cells from the teat and lactiferous sinuses of the udder was examined. Adhesion was detected with bacterial suspensions that produced mannose-sensitive agglutination of guinea-pig red cells. Adhesion to epithelial cells could be inhibited by ...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-11-2-117
更新日期：1978-05-01 00:00:00
abstract：:Enteroaggregative Escherichia coli (EAEC), a highly heterogeneous pathotype of E. coli classified as typical and atypical, are an emerging cause of acute and persistent diarrhea. We aimed to investigate whether population living in rural geographic areas, impacts in the heterogeneity, dissemination and antimicrobial s...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.001120
更新日期：2020-01-01 00:00:00
abstract：:The aim of this study was to evaluate the presence and prevalence of multidrug antibacterial resistance in Helicobacter pylori in Bulgaria from 2005 to 2008. The resistance in 828 untreated adults, 124 treated adults and 105 untreated children was, respectively, 26.5, 50.8 and 16.2% for metronidazole; 18.4, 45.2 and 1...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.009993-0
更新日期：2009-07-01 00:00:00
abstract：:Introduction. Melioidosis, caused by Burkholderia pseudomallei, in endemic areas, poses a challenge for treating the diseased populations without accurate diagnosis, and the disease-specific biomarkers linked with the infection have yet to be reported. Due to the invasive nature of the causative agent, Burkholderia ps...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.001242
更新日期：2020-10-01 00:00:00
abstract：:Faeces from urban children < 2 years old with acute diarrhoeal illness and from non-diarrhoeal infants (controls) were examined for Escherichia coli and other enteropathogens. A total of 990 E. coli isolates from 100 patients and 50 controls was tested for enteropathogenic E. coli (EPEC) serotype (O:H), adherence to H...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-47-9-781
更新日期：1998-09-01 00:00:00
abstract：:Extracts of 19 samples of pus which showed red fluorescence with ultraviolet light were screened for the presence of porphyrins by absorption spectrophotometry. All those which showed spectra typical of metal-free porphyrins were analysed by high performance liquid chromatography to identify the porphyrins present. Th...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-33-1-29
更新日期：1990-09-01 00:00:00
abstract：:Leucocyte-egested material was harvested after the quantitative in-vitro phagocytosis of Neisseria meningitidis by rabbit or mouse polymorphonuclear leucocytes. The egested material was injected subcutaneously into rabbits and followed 24 h later with an intravenous injection of what would by itself have been a non-le...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-9-4-451
更新日期：1976-11-01 00:00:00
abstract：:The aim of this study was to establish bacterial profiles in gastric biopsy specimens from patients with Helicobacter pylori-associated gastritis by means of temporal temperature gradient gel electrophoresis (TTGE) of PCR-amplified 16S rDNA fragments. Specimens from eight patients with asymptomatic gastritis and five ...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/0022-1317-49-9-817
更新日期：2000-09-01 00:00:00
abstract：:Members of the Streptococcus anginosus group (SAG) are frequently involved in pyogenic infections in humans. In the present study, the antimicrobial susceptibility of 141 clinical SAG isolates to six antimicrobial agents was analysed by agar dilution. All isolates were susceptible to penicillin, cefotaxime and vancomy...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.001560-0
更新日期：2009-02-01 00:00:00
abstract：:Some strains of Helicobacter pylori are known to produce an extracellular cytotoxin that causes vacuolation in cultured mammalian cells. Screening for such strains makes use of HeLa cells which may not be sensitive enough to detect minimal changes. The aim of this study was to develop a more sensitive cell line. Vacuo...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-46-8-705
更新日期：1997-08-01 00:00:00
abstract：:This prospective study investigated whether oral candidal carriage in HIV-infected patients was altered by pentamidine therapy. Repeated oral rinses were taken from 56 HIV-positive patients over a 2-year period. Oral candidal carriage was investigated in two groups of patients, one receiving prophylactic pentamidine t...
journal_title：Journal of medical microbiology
pub_type： 临床试验,杂志文章
doi：10.1099/00222615-41-2-95
更新日期：1994-08-01 00:00:00
abstract：:There have been several outbreaks of botulism among poultry and wild birds in Sweden in recent years. The National Veterinary Institute of Sweden (SVA) has identified botulinum neurotoxin (BoNT)/C1 or the mosaic BoNT/C1D using the mouse bioassay. This is believed to be the first report on the application of the Endope...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.031179-0
更新日期：2011-09-01 00:00:00
abstract：:The lipopolysaccharides (LPS) of the 10 species of the genus Bacteroides (sensu stricto) were extracted by the proteinase K method and their resolution compared by several methods of polyacrylamide gel electrophoresis (PAGE). These included sodium dodecyl sulphate (SDS)-PAGE with and without urea, polyacrylamide gradi...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-34-5-253
更新日期：1991-05-01 00:00:00
abstract：:Introduction. Differences between the genomic and virulence profile of Bordetella pertussis circulating strains and vaccine strains are considered as one of the important reasons for the resurgence of whooping cough (pertussis) in the world. Genetically inactivated B. pertussis is one of the new strategies to generate...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.001114
更新日期：2020-01-01 00:00:00
abstract：:To study the influence of normal associated microbiota on systemic immunological responses during experimental Chagas' disease, germ-free and conventional NIH Swiss mice were infected with Y strain of Trypanosoma cruzi. Although no statistical differences in mortality and parasitaemia were found, conventional mice sho...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.45657-0
更新日期：2004-08-01 00:00:00
abstract：:Introduction. PCV2 is a DNA virus that exists widely in pigs and has caused great economic losses to the pig industry worldwide. In the existing commercial PCV2 enzyme-linked immunosorbent assay (ELISA) kits both natural infection with PCV2 and vaccine immunization produce results that are positive for PCV2 Cap antibo...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.001230
更新日期：2020-09-01 00:00:00
abstract：:The increasing incidence of reported scabies in Trinidad, from 24.2/100,000 population in 1984 to 59.5/100,000 in 1985, led to a careful monitoring in 1986 of all patients with post-streptococcal acute glomerulonephritis (PSAGN). There were 181 cases of PSAGN; 84 beta-haemolytic streptococcal isolates from 72 patients...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-32-2-111
更新日期：1990-06-01 00:00:00
abstract：:A large molecular-weight fraction of Pseudomonas aeruginosa culture filtrate protected rats and mice against a lethal infection with a heterologous serotype, and to some extent against Escherichia coli and Listeria monocytogenes. The active components were obtained from cultures grown for several days in a simple synt...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-13-2-201
更新日期：1980-05-01 00:00:00
abstract：:In this study, 90 non-replicate imipenem-resistant Pseudomonas aeruginosa (IRPA) Malaysian isolates collected between October 2005 and March 2008 were subjected to a screening test for detection of the integron and the gene cassette. Class 1 integrons were detected in 54 IRPA clinical isolates, whilst three isolates c...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.029868-0
更新日期：2011-07-01 00:00:00
abstract：:Bacterially-driven mucosal inflammation and the development of periimplantitis can lead to oral implant failure. In this study, initial bacterial adhesion after 2 h, and biofilm formation after 1 day and 3 days, were analysed in situ on novel 3 mol % yttria-stabilized tetragonal zirconia polycrystal samples, as well a...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.000267
更新日期：2016-07-01 00:00:00
abstract：:The distribution of cryptic plasmids among 123 isolates of Providencia stuarti from a hospital ward during a prospective epidemiological study is reported. Two closely related stable plasmids (34 Kb and 36 Kb) were identified by restriction endonuclease digest analysis of plasmid DNA. One or other of these cryptic pla...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-18-2-277
更新日期：1984-10-01 00:00:00
abstract：:Acinetobacter baumannii, Acinetobacter nosocomialis and Acinetobacter pittii are clinically relevant members of the Acinetobacter calcoaceticus-A. baumannii (Acb) complex and important nosocomial pathogens. These three species are genetically closely related and phenotypically similar; however, they differ in their ep...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.071712-0
更新日期：2014-09-01 00:00:00
