Abstract:
:The surface structures of the cell envelopes of 16 clinical isolates of Pseudomonas aeruginosa were examined by electronmicroscopy with the new fixation technique of freeze-substitution. Two types of structures were observed among the organisms. In one group of strains, mostly isolated from blood, a dense fibrous layer c. 30 nm thick was found around the outer-membrane surface, whereas no such structure was observed in the other group of isolates, most of which were from sputum. Lipopolysaccharides extracted from the isolates with a dense fibrous layer were found by SDS-PAGE to have long O-polysaccharide chains, whereas strains without such a layer mostly had lipopolysaccharides that lacked high mol. wt. O-polysaccharide chains.
journal_name
J Med Microbioljournal_title
Journal of medical microbiologyauthors
Misumi H,Umeda A,Eguchi K,Okada K,Sawae Y,Niho Y,Amako Kdoi
10.1099/00222615-40-4-282subject
Has Abstractpub_date
1994-04-01 00:00:00pages
282-7issue
4eissn
0022-2615issn
1473-5644journal_volume
40pub_type
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