Assessment of a new selective medium for the isolation of Salmonellae.

abstract：:The effect of passive immunotherapy with antisera against heat-killed Pseudomonas aeruginosa and three of its exo-enzymes (elastase, alkaline protease and exotoxin A) in gut-derived P. aeruginosa sepsis was evaluated. Mice were given a suspension of P. aeruginosa strain D4 in their drinking water, together with ampici...

journal_title：Journal of medical microbiology

authors： Matsumoto T,Furuya N,Tateda K,Miyazaki S,Ohno A,Ishii Y,Hirakata Y,Yamaguchi K

更新日期：1999-08-01 00:00:00

abstract：:Staphylococcus aureus can invade epithelial cells, and the host-cell receptor α(5)β(1) integrin is thought to mediate this process. The aim of this study was to investigate S. aureus invasion of epithelial cell lines derived from oral (H357), skin (UP) and nasopharyngeal (Detroit 562) sites and to determine whether an...

journal_title：Journal of medical microbiology

authors： Ridley RA,Douglas I,Whawell SA

更新日期：2012-12-01 00:00:00

abstract：:Interaction of human polymorphonuclear leucocytes (PMNL) and influenza virus (IFV) was studied in vitro. At 0 degree C, the viral particles were bound extensively to the surface of the PMNL membrane with a ratio of about 1000 virus particles to a single PMNL. The binding was sensitive to neuraminidase, suggesting atta...

journal_title：Journal of medical microbiology

authors： Yamamoto K,Suzuki K,Suzuki K,Mizuno S

更新日期：1989-03-01 00:00:00

abstract：:Campylobacter jejuni, when left on blood agar for prolonged periods, was found to survive better in air than under micro-aerobic conditions. After a period of 2-3 days in air, all strains of C. jejuni examined grew freely in air on subculture, and could be further subcultured apparently indefinitely in air. This adapt...

journal_title：Journal of medical microbiology

authors： Jones DM,Sutcliffe EM,Rios R,Fox AJ,Curry A

更新日期：1993-02-01 00:00:00

abstract：:Intestinal absorption of arbutin (p-hydroxyphenyl-beta-glucoside), a non-metabolised analogue of d-glucose, and net flux of water out of the small intestinal lumen were found to be decreased in vitro in rats fed with Candida albicans. In rats on a protein-deficient diet and fed C. albicans there was net secretion of w...

journal_title：Journal of medical microbiology

authors： Burke V,Gracey M

更新日期：1980-02-01 00:00:00

abstract：:Rickettsia conorii infection is endemic in the Mediterranean basin, where it is known as Mediterranean spotted fever, also known as Boutonneuse fever and Marseilles fever. We report the case of a 66-year-old diabetic man who presented a severe form of the disease, complicated by acute renal failure, thrombocytopenia a...

journal_title：Journal of medical microbiology

authors： Aliaga L,Sánchez-Blázquez P,Rodríguez-Granger J,Sampedro A,Orozco M,Pastor J

更新日期：2009-04-01 00:00:00

abstract：:Faecal specimens from 843 cases of diarrhoea in the community were tested for the presence of Clostridium difficile cytotoxin and Clostridium perfringens enterotoxin. C. difficile cytotoxin was detected in faecal specimens from 0.6 % of cases aged at least 2 years by using a Vero cell assay. Factors associated with de...

journal_title：Journal of medical microbiology

authors： Forward LJ,Tompkins DS,Brett MM

更新日期：2003-09-01 00:00:00

abstract：:In serological typing of Klebsiella pneumoniae strains from human, equine and environmental sources, the capsular identity of many isolates could not be determined because of serological cross-reactivity. A panel of 91 bacteriophages able to lyse each of the 77 capsular serotypes of K. pneumoniae was isolated and test...

journal_title：Journal of medical microbiology

authors： Pieroni P,Rennie RP,Ziola B,Deneer HG

更新日期：1994-12-01 00:00:00

abstract：:Experiments were performed to determine whether a modern flow cytometer could be used to study bacterial populations in suspension, with particular reference to their morphological characteristics and their responses to antibiotics. The FACScan, a commercial benchtop flow cytometer fitted with an air-cooled laser, des...

journal_title：Journal of medical microbiology

authors： Gant VA,Warnes G,Phillips I,Savidge GF

更新日期：1993-08-01 00:00:00

abstract：PURPOSE:The presence of alginate-overproducing (Alg+) strains of Pseudomonas aeruginosa in cystic fibrosis patients is indicative of chronic infection. The Alg+ phenotype is generally due to a mutation in the mucA gene, encoding an innermembrane protein that sequesters AlgT/U, the alginate-specific sigma factor. AlgT/U...

journal_title：Journal of medical microbiology

authors： Pandey S,Delgado C,Kumari H,Florez L,Mathee K

更新日期：2018-08-01 00:00:00

abstract：:The opsonic requirements of Helicobacter pylori were investigated in a series of experiments with human polymorphonuclear leucocytes (PMNL). Pre-incubation of H. pylori with pooled normal human serum (NHS) in concentrations of 5-20% significantly increased the uptake of radiolabelled bacteria by PMNL. Treatment of the...

journal_title：Journal of medical microbiology

authors： McKinlay AW,Young A,Russell RI,Gemmell CG

更新日期：1993-03-01 00:00:00

abstract：:A novel bunyavirus associated with fever, thrombocytopenia and leukopenia syndrome (FTLS) was discovered in Henan Province, China. Here, we report the development of an assay for this novel bunyavirus based on real-time reverse transcription PCR (RT-PCR). The assay exhibited high sensitivity and specificity without cr...

journal_title：Journal of medical microbiology

authors： Huang X,Liu L,Du Y,Ma H,Mu Y,Pengzhi Wang,Ma H,Tang X,Wang H,Kang K,Zhang S,Wu W,Chen H,Liu G,Yang Y,Yongqiangj,Jiang,Xu B,Chen W

更新日期：2013-07-01 00:00:00

abstract：:The response of Legionella pneumophila to antibiotics that inhibit cell-wall, protein and DNA synthesis was examined by electronmicroscopy, MIC estimations and viable counts. Ampicillin, cefotaxime, methicillin, erythromycin, rifampicin and ciprofloxacin, each used separately at 20 times their respective MIC values, s...

journal_title：Journal of medical microbiology

authors： Rodgers FG,Tzianabos AO,Elliott TS

更新日期：1990-01-01 00:00:00

abstract：:Antigen participating in cell-mediated immunity was isolated from a sonic extract of Mycobacterium tuberculosis H37Rv. The purified antigen had a mol. wt 150 000-200 000, based upon its Rf in 7.5% polyacrylamide gel in the presence of sodium dodecyl sulphate. In simple agarose gel electrophoresis it had an Rf of 0.85....

journal_title：Journal of medical microbiology

authors： Hattikudur S,Kamat RS

更新日期：1984-08-01 00:00:00

abstract：:Four hundred and twenty-seven Escherichia coli isolates from 427 cases of infantile diarrhoea in Poland, belonging to serogroups O18, O26, O44, O86, O126 and O127 and 150 E. coli isolates from 52 healthy children were examined for selected virulence properties. The presence of the plasmid pAA, a plasmid encoding enter...

journal_title：Journal of medical microbiology

authors： Paciorek J

更新日期：2002-07-01 00:00:00

abstract：:Clostridium perfringens has been reported as the cause of up to 15% of cases of antibiotic-associated diarrhoea (AAD) and may be diagnosed by detection of enterotoxin (CPEnt) in faeces. The performance of a commercial ELISA method for CPEnt, with culture and PCR methods to confirm the presence of enterotoxigenic C. pe...

journal_title：Journal of medical microbiology

authors： Asha NJ,Wilcox MH

更新日期：2002-10-01 00:00:00

abstract：:Diabetic mice were significantly more susceptible than normal mice to bladder infection with Enterobacter cloacae or Serratia marcescens but not to intraperitoneal infection. Normal urine inhibited the growth of E. cloacae and S. marcescens, whereas urine from diabetic mice permitted multiplication. The addition of ur...

journal_title：Journal of medical microbiology

authors： Obana Y,Nishino T

更新日期：1989-10-01 00:00:00

abstract：:Historically, enteropathogenic Escherichia coli (EPEC) are a well-known cause of outbreaks of infantile diarrhoea associated with morbidity and mortality in England. The aim of this study was to provide an update on the microbiology and epidemiology of strains of EPEC in England between 2010 and 2012. A wide range of ...

journal_title：Journal of medical microbiology

authors： Sakkejha H,Byrne L,Lawson AJ,Jenkins C

更新日期：2013-10-01 00:00:00

abstract：:Clostridium difficile is the most common cause of antibiotic-associated diarrhoea. Antibiotics are presumed to disturb the normal intestinal microbiota, leading to depletion of the barrier effect and colonization by pathogenic bacteria. This first step of infection includes adherence to epithelial cells. We investigat...

journal_title：Journal of medical microbiology

authors： Denève C,Deloménie C,Barc MC,Collignon A,Janoir C

更新日期：2008-06-01 00:00:00

abstract：PURPOSE:In this study, we aimed to investigate the genomic characteristics and evolution of pathogenicity islands of an enteropathogenic Escherichia coli (EPEC) strain, and to obtain a transcriptional profile of EPEC under different concentrations of ciprofloxacin using microarray analysis. METHODOLOGY:The complete EP...

journal_title：Journal of medical microbiology

authors： Chen Z,Cheng H,Pan W,Zheng J,Li D,Lin F,Yu Z,Deng Q

更新日期：2018-09-01 00:00:00

abstract：:The microtechnique of Galton et al. (1965) for the routine screening of blood samples for leptospiral antibodies was used to examine more than 8000 serum samples from animals that had aborted and from herds in which leptospiral antibodies had previously been demonstrated. The method gave consistently accurate results....

journal_title：Journal of medical microbiology

authors： Lewis J

更新日期：1978-08-01 00:00:00

abstract：:The potential of incorporating a real-time PCR for amplification and detection of 16S rRNA gene signatures directly from clinical samples was assessed as a tool for diagnostics. Universal PCR primers spanning short variable regions (~500 bp) were optimized for real-time PCR and tested in comparison with a longer fragm...

journal_title：Journal of medical microbiology

authors： Edwards KJ,Logan JMJ,Langham S,Swift C,Gharbia SE

更新日期：2012-05-01 00:00:00

abstract：:CaNdt80p, the Candida albicans homologue of the Saccharomyces cerevisiae transcription factor ScNdt80p, has been identified as a positive regulator of CDR1, which encodes an efflux pump involved in drug resistance in C. albicans. To investigate the involvement of the putative DNA-binding domain of CaNdt80p in drug res...

journal_title：Journal of medical microbiology

authors： Wang JS,Yang YL,Wu CJ,Ouyang KJ,Tseng KY,Chen CG,Wang H,Lo HJ

更新日期：2006-10-01 00:00:00

abstract：:Extracts of 19 samples of pus which showed red fluorescence with ultraviolet light were screened for the presence of porphyrins by absorption spectrophotometry. All those which showed spectra typical of metal-free porphyrins were analysed by high performance liquid chromatography to identify the porphyrins present. Th...

journal_title：Journal of medical microbiology

authors： Brazier JS

更新日期：1990-09-01 00:00:00

abstract：:The aim of this study was to assess the primary and combined resistances of Helicobacter pylori isolates obtained from paediatric patients in 2000-2001 to seven antimicrobial agents. Resistance rates of pre-treatment isolates from 115 children were investigated by the limited agar dilution method alone and by the E-te...

journal_title：Journal of medical microbiology

authors： Boyanova L,Koumanova R,Gergova G,Popova M,Mitov I,Kovacheva Y,Derejian S,Katsarov N,Nikolov R,Krastev Z

更新日期：2002-09-01 00:00:00

abstract：:The bactericidal activity of compound DR-3355, an optically active isomer of ofloxacin, was measured against Escherichia coli, Staphylococcus aureus and S. epidermidis, in nutrient broth and in phosphate-buffered saline. DR-3355 was found to be approximately twice as active as ofloxacin in terms of the concentration a...

journal_title：Journal of medical microbiology

authors： Lewin CS,Amyes SG

更新日期：1989-11-01 00:00:00

abstract：:The strains of Proteus species found in significant numbers and as pure cultures in urine from 217 individuals were isolated, identified to species level and typed for proticine production (P type) and proticine sensitivity (S type) to give their P/S type. Urinary-tract infections with Proteus, principally P. mirabili...

journal_title：Journal of medical microbiology

authors： Senior BW

更新日期：1979-02-01 00:00:00

abstract：:This study used a previously described multiplex PCR-based reverse line blot (mPCR/RLB) assay to assess the prevalence and distribution of 14 urogenital pathogens or putative pathogens, namely Neisseria gonorrhoeae, Chlamydia trachomatis, Mycoplasma genitalium, Mycoplasma hominis, Trichomonas vaginalis, Gardnerella va...

journal_title：Journal of medical microbiology

authors： McKechnie ML,Hillman RJ,Jones R,Lowe PC,Couldwell DL,Davies SC,King F,Kong F,Gilbert GL

更新日期：2011-07-01 00:00:00

abstract：:In January 2015, Public Health England and the United Kingdom (UK) Ministry of Defence investigated cases of diarrhoea and fever in military personnel recently returned to the UK after supporting the response to the Ebola epidemic in Sierra Leone. Tests for Ebola virus infection were negative. PCR tests detected the i...

journal_title：Journal of medical microbiology

authors： Todkill D,Pudney R,Terrell A,Tuck J,Hutley E,Dallman TJ,Jenkins C,Manuel R,Wade M,Puleston R,Hawker J

更新日期：2018-11-01 00:00:00

abstract：:The acid end-products of 185 isolates from the family Bacteroidaceae were separated and analysed by gas-liquid chromatography on broth cultures. Different media were evaluated and definitive studies were performed in a fully supplemented complex medium. The limitations of this approach to the identification of a wide ...

journal_title：Journal of medical microbiology

authors： Deacon AG,Duerden BI,Holbrook WP

更新日期：1978-05-01 00:00:00