Detection of Clostridium difficile cytotoxin and Clostridium perfringens enterotoxin in cases of diarrhoea in the community.

Abstract:

:Faecal specimens from 843 cases of diarrhoea in the community were tested for the presence of Clostridium difficile cytotoxin and Clostridium perfringens enterotoxin. C. difficile cytotoxin was detected in faecal specimens from 0.6 % of cases aged at least 2 years by using a Vero cell assay. Factors associated with detection of C. difficile cytotoxin were antibiotic therapy, age over 60 years and living in a home with other elderly people. Three methods were used for the detection of C. perfringens enterotoxin: a Vero cell assay, a commercial (TechLab) enzyme immunoassay (EIA) and an in-house EIA. The lower level of detection of pure C. perfringens enterotoxin in buffer was 0.01 micro g ml(-1) by the TechLab EIA and 1.0 micro g ml(-1) by the Vero cell assay. C. perfringens enterotoxin was detected by using the TechLab EIA in faecal specimens from 2.5 % of cases. This commercial EIA was less sensitive than the in-house EIA, detecting only 31 % of positive cases, but was specific and could be used for outbreak investigation by routine diagnostic laboratories. Age over 60 years was a factor associated with C. perfringens enterotoxin detection; this age group may be targeted for testing.

journal_name

J Med Microbiol

authors

Forward LJ,Tompkins DS,Brett MM

doi

10.1099/jmm.0.05119-0

keywords:

subject

Has Abstract

pub_date

2003-09-01 00:00:00

pages

753-757

issue

Pt 9

eissn

0022-2615

issn

1473-5644

journal_volume

52

pub_type

杂志文章
  • Characteristics of antibiotic-resistant Escherichia coli in the rectum of healthy school-children.

    abstract::During a 3-year period, 771 rectal swabs were taken from abacteriuric school-children. Out of 709 E. coli strains, each isolated from one faecal specimen, 102 were found to be resistant to one or more antibacterial agents, and 607 to be fully sensitive. Another 204 resistant strains were found by selection for antibio...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-10-3-299

    authors: Lidin-Janson G,Falsen E,Jodal U,Kaijser B,Lincoln K

    更新日期:1977-08-01 00:00:00

  • The use of bacteriophages to differentiate serologically cross-reactive isolates of Klebsiella pneumoniae.

    abstract::In serological typing of Klebsiella pneumoniae strains from human, equine and environmental sources, the capsular identity of many isolates could not be determined because of serological cross-reactivity. A panel of 91 bacteriophages able to lyse each of the 77 capsular serotypes of K. pneumoniae was isolated and test...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-41-6-423

    authors: Pieroni P,Rennie RP,Ziola B,Deneer HG

    更新日期:1994-12-01 00:00:00

  • Comparative analysis of 16S RNA nucleotide sequences of Anaplasma phagocytophilum detected in the blood of horses from various parts of Europe.

    abstract::The aim of this study was to conduct a comparative analysis of the 16S rRNA gene fragment nucleotide sequences for Anaplasma phagocytophilum strains detected in the blood of horses from various parts of Europe. The study comprised 234 horses that had had contact with ticks. Using PCR, the genetic material of A. phagoc...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.058636-0

    authors: Dzięgiel B,Adaszek L,Winiarczyk M,García-Bocanegra I,Carbonero A,Dębiak P,Winiarczyk S

    更新日期:2013-12-01 00:00:00

  • Hospital clonal dissemination of Enterobacter aerogenes producing carbapenemase KPC-2 in a Chinese teaching hospital.

    abstract::Carbapenems are first-line agents for the treatment of serious nosocomial infections caused by multidrug-resistant Enterobacteriaceae. However, resistance to carbapenems has increased dramatically among Enterobacteriaceae in our hospital. In this study, we report clonal dissemination caused by carbapenem-resistant Ent...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.064865-0

    authors: Qin X,Yang Y,Hu F,Zhu D

    更新日期:2014-02-01 00:00:00

  • Factors determining the susceptibility of mice to experimental phycomycosis.

    abstract::Various factors influencing the susceptibility of C3H mice to lethal infection by the phycomycete Absidia ramosa were examined. Mice 19-21 days old were exposed to graded doses of A. ramosa spores by various routes. After intravenous inoculation with doses in excess of 10(3) viable units, a variable proportion of mice...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-8-4-551

    authors: Corbel MJ,Eades SM

    更新日期:1975-11-01 00:00:00

  • Prevalence of Anaplasma phagocytophilum and its coinfection with Borrelia afzelii in Ixodes ricinus and Ixodes persulcatus ticks inhabiting Tver Province (Russia) - a sympatric region for both tick species.

    abstract::Human granulocytic anaplasmosis (HGA) and Lyme borreliosis (LB) are tick-borne infectious diseases caused by Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato species, respectively. In this study, p44/msp2 paralogues specific to A. phagocytophilum and 5S-23S rRNA gene-intergenic spacers specific to B. burg...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.47721-0

    authors: Masuzawa T,Kharitonenkov IG,Okamoto Y,Fukui T,Ohashi N

    更新日期:2008-08-01 00:00:00

  • Epigallocatechin gallate suppresses expression of receptor activator of NF-kappaB ligand (RANKL) in Staphylococcus aureus infection in osteoblast-like NRG cells.

    abstract::Catechin, a constituent of tea, possesses various bioactivities. In particular, the most abundant catechin in tea is epigallocatechin gallate (EGCg), which has an anti-inflammatory effect. In the present study, the usability of EGCg for osteomyelitis treatment was examined. Osteomyelitis is a difficult disease to cure...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.47029-0

    authors: Ishida I,Kohda C,Yanagawa Y,Miyaoka H,Shimamura T

    更新日期:2007-08-01 00:00:00

  • Increased drug resistance of meticillin-resistant Staphylococcus aureus biofilms formed on a mouse dermal chip model.

    abstract:PURPOSE:Meticillin-resistant Staphylococcus aureus (MRSA) biofilm formation in humans is of serious clinical concern. Previous in vitro studies have been performed with biofilms grown only on inorganic substrates; therefore, we investigated the vancomycin (VCM) resistance of MRSA biofilms grown on skin tissue. METHODO...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000461

    authors: Jimi S,Miyazaki M,Takata T,Ohjimi H,Akita S,Hara S

    更新日期:2017-04-01 00:00:00

  • A mechanism of pathogenicity of "Streptococcus milleri group" in pulmonary infection: synergy with an anaerobe.

    abstract::The relationship between Streptococcus constellatus, one of the species of the "Streptococcus milleri group", and Prevotella intermedia was studied in a model of pneumonia in mice and in vitro to elucidate mechanisms of pathogenicity in "S. milleri group"-associated pulmonary infection. Acute pneumonia with or without...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-40-2-118

    authors: Shinzato T,Saito A

    更新日期:1994-02-01 00:00:00

  • Isolation of haemin-binding proteins of Neisseria gonorrhoeae.

    abstract::Although Neisseria gonorrhoeae can use haem as the sole exogenous iron source for growth in vitro, the mechanism of haem-iron uptake in the gonococcus is unknown. Two haemin-binding proteins (HmBPs) of 97 and 44 Kda were isolated by batch ligand affinity-chromatography from whole cells or total membranes of gonococci ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-36-2-121

    authors: Lee BC

    更新日期:1992-02-01 00:00:00

  • Hyperinvasiveness and increased intercellular spread of Listeria monocytogenes sequence type 1 are independent of listeriolysin S, internalin F and internalin J1.

    abstract:PURPOSE:Listeria monocytogenes is a genetically heterogeneous species, which is divided into evolutionary lineages and clonal complexes (CCs). Not all L. monocytogenes isolates are equally likely to cause disease, with CC1, and in particular sequence type (ST) 1, being the most prevalent complex in human and ruminant i...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000529

    authors: Rupp S,Bärtschi M,Frey J,Oevermann A

    更新日期:2017-07-01 00:00:00

  • Severe community-acquired pneumococcal pneumonia (CAP) - a potentially fatal illness.

    abstract::Two cases of severe community-acquired pneumococcal pneumonia (CAP) admitted to our hospital within 24 h are described, both in young males. These two cases illustrate the usefulness of the British Thoracic Society severity criteria and serve to emphasize the importance of early recognition of adverse physiology in cr...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.05338-0

    authors: Kelly MG,Sharkey RA,Moles KW,Daly JG

    更新日期:2004-01-01 00:00:00

  • Antimicrobial resistance in group B streptococcus: the Australian experience.

    abstract::Intrapartum chemoprophylaxis for pregnant group B streptococcus (GBS) carriers reduces vertical transmission, with a resultant decrease in neonatal as well as maternal morbidity from invasive GBS infection. Current Australian guidelines recommend penicillin for intrapartum prophylaxis of GBS carriers, with erythromyci...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.022616-0

    authors: Garland SM,Cottrill E,Markowski L,Pearce C,Clifford V,Ndisang D,Kelly N,Daley AJ

    更新日期:2011-02-01 00:00:00

  • Significance of Cryptosporidium in acute diarrhoea in North-Eastern India.

    abstract::In a hospital-based study, stool samples from 2095 patients of all ages were examined for different fungal, protozoal and bacterial enteropathogens over a period of 2 years (July 1994-June 1996). Cryptosporidium was detected in 151 specimens (7.2%) and was the third commonest pathogen found. The highest prevalence of ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-48-6-523

    authors: Nath G,Choudhury A,Shukla BN,Singh TB,Reddy DCS

    更新日期:1999-06-01 00:00:00

  • Inhibition of streptolysin O by allicin - an active component of garlic.

    abstract::Streptolysin O (SLO) is a potent cytolytic toxin produced by almost all strains of group A streptococci and is considered an important virulence factor for this organism. In this study we investigated the effect of allicin and aqueous garlic extracts on the haemolytic activity of SLO. All tested materials potentially ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.019539-0

    authors: Arzanlou M,Bohlooli S

    更新日期:2010-09-01 00:00:00

  • Application of terminal RFLP analysis to characterize oral bacterial flora in saliva of healthy subjects and patients with periodontitis.

    abstract::Terminal restriction fragment-length polymorphism (T-RFLP) analysis was applied to characterize oral bacterial flora in saliva from 18 healthy subjects and 18 patients with periodontitis. The 16S rRNA genes (rDNAs) of oral bacteria and spirochaetes in saliva were amplified by PCR with a 6'carboxy-fluorescein (6-FAM)-l...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.04991-0

    authors: Sakamoto M,Takeuchi Y,Umeda M,Ishikawa I,Benno Y

    更新日期:2003-01-01 00:00:00

  • Analysis of Epstein-Barr virus and cellular gene expression during the early phases of Epstein-Barr virus lytic induction.

    abstract::In order to develop novel host/pathogen real-time PCR assays for routine diagnostic use, early gene expression patterns from both Epstein-Barr virus (EBV) and Raji cells were examined after inducing the lytic life cycle using 12-O-tetradecanoyl-13-phorbol ester and sodium butyrate. Real-time PCR identified several hig...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000352

    authors: Auburn H,Zuckerman M,Smith M

    更新日期:2016-11-01 00:00:00

  • Agglutination of "Streptococcus milleri" by lectins.

    abstract::The agglutination of 218 clinical isolates and three ATCC type strains of "Streptococcus milleri" was tested with 25 different lectins from plants and fungi. An agglutination reaction with one or more lectins was observed with 42 isolates when the cells were untreated. After trypsinisation of the bacteria, 109 strains...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-41-1-14

    authors: Kellens JT,Jacobs JA,Peumans WJ,Stobberingh EE

    更新日期:1994-07-01 00:00:00

  • Analysis of mutations in the gyrA and gyrB genes and their association with the resistance of Mycobacterium tuberculosis to levofloxacin, moxifloxacin and gatifloxacin.

    abstract::The purpose of the present study was to analyse mutations in the gyrA and gyrB genes of Mycobacterium tuberculosis and define the possible correlation between these mutations and resistance to levofloxacin (LVX), moxifloxacin (MFX) and gatifloxacin (GAT), based on their MICs. One hundred and forty-two M. tuberculosis ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.046821-0

    authors: Nosova EY,Bukatina AA,Isaeva YD,Makarova MV,Galkina KY,Moroz AM

    更新日期:2013-01-01 00:00:00

  • Bacteriophages in Helicobacter (Campylobacter) pylori.

    abstract::Bacteriophages in different stages of maturation were found in thin sections of a clinical isolate of Helicobacter (Campylobacter) pylori. Mature phage heads measured 70 x 60 nm and the tail at least 120 nm. Lysogeny was maintained during subculture on blood agar for more than 3 months after isolation from a gastric b...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-32-2-101

    authors: Schmid EN,von Recklinghausen G,Ansorg R

    更新日期:1990-06-01 00:00:00

  • Zinc enhances the phototoxic effect of blue light against malodour-producing bacteria in an experimental oral biofilm.

    abstract::Oral malodour is thought to be caused mainly by the production of volatile sulfide compounds (VSCs) by anaerobic Gram-negative oral bacteria. Previous studies have shown that these bacteria are susceptible to blue light (400-500 nm wavelength). In the present study, we tested the effect of blue light in the presence o...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.075044-0

    authors: Sterer N,Jeffet U,Dadoun A,Greenstein RB,Kohavi D

    更新日期:2014-08-01 00:00:00

  • Synergistic potential of Juniperus communis and Helichrysum italicum essential oils against nontuberculous mycobacteria.

    abstract:OBJECTIVE:The present study evaluated the possible synergistic antimycobacterial interactions of Juniperus communis and Helichrysum italicum essential oils (EO). METHODS:Antimycobacterial potential was tested against Mycobacterium avium and Mycobacterium intracellulare using broth and water dilution method and checker...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000962

    authors: Peruč D,Tićac B,Abram M,Broznić D,Štifter S,Staver MM,Gobin I

    更新日期:2019-05-01 00:00:00

  • Adhesion of fimbriate Escherichia coli to bovine mammary-gland epithelial cells in vitro.

    abstract::The adhesion of bovine and a human isolate of Escherichia coli to epithelial cells from the teat and lactiferous sinuses of the udder was examined. Adhesion was detected with bacterial suspensions that produced mannose-sensitive agglutination of guinea-pig red cells. Adhesion to epithelial cells could be inhibited by ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-11-2-117

    authors: Harper M,Turvey A,Bramley AJ

    更新日期:1978-05-01 00:00:00

  • Role of fibronectin-binding protein A in Clostridium difficile intestinal colonization.

    abstract::Clostridium difficile is a frequent cause of severe, recurrent, post-antibiotic diarrhoea and pseudomembranous colitis. Its pathogenicity is mediated mainly by two toxins, TcdA and TcdB. However, different adhesins have also been described as important colonization factors which are implicated in the first step of the...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.029553-0

    authors: Barketi-Klai A,Hoys S,Lambert-Bordes S,Collignon A,Kansau I

    更新日期:2011-08-01 00:00:00

  • Prevalence of erythromycin and clindamycin resistance among clinical isolates of the Streptococcus anginosus group in Germany.

    abstract::Members of the Streptococcus anginosus group (SAG) are frequently involved in pyogenic infections in humans. In the present study, the antimicrobial susceptibility of 141 clinical SAG isolates to six antimicrobial agents was analysed by agar dilution. All isolates were susceptible to penicillin, cefotaxime and vancomy...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.001560-0

    authors: Asmah N,Eberspächer B,Regnath T,Arvand M

    更新日期:2009-02-01 00:00:00

  • Human bovine tuberculosis - remains in the differential.

    abstract::Mycobacterium bovis is a pathogen of cattle. The unpasteurized milk of affected cattle is a source of infection in humans. Despite the screening of cattle and the pasteurization of milk, M bovis has not been eradicated. A high index of clinical suspicion is needed in symptomatic patients with a history of possible exp...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.020511-0

    authors: Bilal S,Iqbal M,Murphy P,Power J

    更新日期:2010-11-01 00:00:00

  • Plasmid-mediated resistance to gentamicin in Staphylococcus aureus: the involvement of a transposon.

    abstract::Resistance to gentamicin, tobramycin and kanamycin (GmrTmrKmr) in strains of Staphylococcus aureus isolated from clinical sources in Australia is mediated by a 4.7 kb transposable element, designated Tn4001. A 2.5 kb HindIII fragment which maps symmetrically within Tn4001, and encompasses the aminoglycoside-resistance...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-23-2-101

    authors: Lyon BR,Gillespie MT,Byrne ME,May JW,Skurray RA

    更新日期:1987-03-01 00:00:00

  • Wide genetic heterogeneity and low antimicrobial resistance of enteroaggregative Escherichia coli isolates from several rural communities.

    abstract::Enteroaggregative Escherichia coli (EAEC), a highly heterogeneous pathotype of E. coli classified as typical and atypical, are an emerging cause of acute and persistent diarrhea. We aimed to investigate whether population living in rural geographic areas, impacts in the heterogeneity, dissemination and antimicrobial s...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.001120

    authors: Guerrieri CG,Monfardini MV,Silva EA,Bueno de Freitas L,Schuenck RP,Spano LC

    更新日期:2020-01-01 00:00:00

  • Cloning and sequencing the endocarditis immunodominant antigen of Streptococcus sobrinus strain MUCOB 263.

    abstract::Immunoblotting sera from cases of Streptococcus mutans or S. sobrinus endocarditis against an extract from S. sobrinus strain MUCOB 263 had identified three immunodominant antigenic bands at 190, 200 and 220 kDa. A lambda ZAPII DNA library was produced from the sheared genomic DNA of S. sobrinus MUCOB 263 and six iden...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-40-5-330

    authors: Brooks W,Burnie JP

    更新日期:1994-05-01 00:00:00

  • The prevalence of urogenital micro-organisms detected by a multiplex PCR-reverse line blot assay in women attending three sexual health clinics in Sydney, Australia.

    abstract::This study used a previously described multiplex PCR-based reverse line blot (mPCR/RLB) assay to assess the prevalence and distribution of 14 urogenital pathogens or putative pathogens, namely Neisseria gonorrhoeae, Chlamydia trachomatis, Mycoplasma genitalium, Mycoplasma hominis, Trichomonas vaginalis, Gardnerella va...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.029108-0

    authors: McKechnie ML,Hillman RJ,Jones R,Lowe PC,Couldwell DL,Davies SC,King F,Kong F,Gilbert GL

    更新日期:2011-07-01 00:00:00