Increased drug resistance of meticillin-resistant Staphylococcus aureus biofilms formed on a mouse dermal chip model.


PURPOSE:Meticillin-resistant Staphylococcus aureus (MRSA) biofilm formation in humans is of serious clinical concern. Previous in vitro studies have been performed with biofilms grown only on inorganic substrates; therefore, we investigated the vancomycin (VCM) resistance of MRSA biofilms grown on skin tissue. METHODOLOGY:We established a novel tissue substrate model, namely MRSA grown on segments of mouse skin tissue (dermal chips, DCs), and compared its resistance capacity against VCM with that of MRSA biofilms grown on plastic chips (PCs).Results/Key findings. For one MRSA isolate, we found that the VCM MIC was identical (1.56 µg ml-1) for planktonic cultures and for biofilms-formed on PCs (PC-BF), although the minimum bactericidal concentration (MBC) increased to 6.25 µg ml-1 in PC-BF. On the contrary, the MIC and MBC for biofilms formed on DCs (DC-BF) significantly increased (25 and 50 µg ml-1, respectively). Furthermore, the minimum biofilm-eradicating concentration was higher for DC-BF (100 µg ml-1) than for PC-BF (25 µg ml-1). Using six MRSA strains, we found that in PC-BF, the c.f.u. number decreased with increasing VCM concentration, whereas in DC-BF, it greatly increased until the MIC was reached, accompanied by the formation of large colonies, thicker bacterial walls and the presence of many mitotic cells. CONCLUSION:Our results indicate that the VCM resistance of MRSA was greater in DC-BF. We conclude that DCs may provide a specific environment for MRSA that enhances bacterial growth under cytotoxic VCM concentrations, and might be useful for the study of skin wound infections and the effects of antimicrobial drugs.


J Med Microbiol


Jimi S,Miyazaki M,Takata T,Ohjimi H,Akita S,Hara S




Has Abstract


2017-04-01 00:00:00












  • Identification by PCR of Helicobacter pylori in subgingival plaque of adult periodontitis patients.

    abstract::The PCR was used to detect the presence of Helicobacter pylori in subgingival plaque samples from patients with adult periodontitis. Primers based upon the 16S ribosomal RNA (rRNA) gene sequence of H. pylori were used in a single round of PCR to amplify a 295-bp DNA fragment and the identity of the amplified products ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Riggio MP,Lennon A

    更新日期:1999-03-01 00:00:00

  • Identification of an immunogenic 18-kDa protein of Helicobacter pylori by alkaline phosphatase gene fusions.

    abstract::The use of alkaline phosphatase fusion methodology to identify Helicobacter pylori exported proteins enabled the identification of an open reading frame (ORF) encoding a highly immunogenic, previously uncharacterised exported protein. The predicted aminoacid sequence displays a typical N-terminal signal peptide and co...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Oliaro J,Johnson RD,Chen W,Chadwick VS,Murray A

    更新日期:2000-07-01 00:00:00

  • Comparison of PCR, culturing and Pap smear microscopy for accurate diagnosis of genital Actinomyces.

    abstract::Members of the genus Actinomyces, Gram-positive, non-spore-forming anaerobic bacteria, are normal inhabitants of the mucosal surfaces of the oral, gastrointestinal and genital tracts. Identification of these bacteria using conventional methods is generally difficult because of their complex transport and growth requir...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Kaya D,Demirezen Ş,Hasçelik G,Gülmez Kıvanç D,Beksaç MS

    更新日期:2013-05-01 00:00:00

  • Transferrin receptors of Neisseria meningitidis: promising candidates for a broadly cross-protective vaccine.

    abstract::Production of a meningococcal vaccine capable of generating long-lasting immunity in all age groups is still a high priority worldwide. Iron-regulated outer-membrane proteins have attracted considerable attention in recent years and it has become increasingly evident that the meningococcal transferrin-binding proteins...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章,评审


    authors: Ala'Aldeen DA

    更新日期:1996-04-01 00:00:00

  • The classical Bordetella species and MALDI-TOF technology: a brief experience.

    abstract:PURPOSE:The aim of this work was to evaluate and optimize the identification of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica (usually known as the classical Bordetella species) using Bruker Biotyper matrix-assisted laser desorption ionization/time-of-flight mass spectrometry (MALDI-TOF M...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Zintgraff J,Irazu L,Lara CS,Rodriguez M,Santos M

    更新日期:2018-12-01 00:00:00

  • Identification, quantification and subtyping of Gardnerella vaginalis in noncultured clinical vaginal samples by quantitative PCR.

    abstract::Gardnerella vaginalis is an important component of the human vaginal microflora. It is proposed to play a key role in the pathogenesis of bacterial vaginosis (BV), the most common vaginal condition. Here we describe the development, validation and comparative analysis of a novel molecular approach capable of G. vagina...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Balashov SV,Mordechai E,Adelson ME,Gygax SE

    更新日期:2014-02-01 00:00:00

  • Wild birds as biological indicators of environmental pollution: antimicrobial resistance patterns of Escherichia coli and enterococci isolated from common buzzards (Buteo buteo).

    abstract::A total of 36 Escherichia coli and 31 enterococci isolates were recovered from 42 common buzzard faecal samples. The E. coli isolates showed high levels of resistance to streptomycin and tetracycline. The following resistance genes were detected: bla(TEM) (20 of 22 ampicillin-resistant isolates), tet(A) and/or tet(B) ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Radhouani H,Poeta P,Gonçalves A,Pacheco R,Sargo R,Igrejas G

    更新日期:2012-06-01 00:00:00

  • In vitro assessment of the APTIMA Combo 2 assay for the detection of Chlamydia trachomatis using highly purified elementary bodies.

    abstract::The Gen-Probe APTIMA Combo 2 assay has previously been reported to have a high sensitivity. The end point of this assay was evaluated using highly purified chlamydial elementary bodies (EBs). The performance of the APTIMA Combo 2 assay was compared with a commercially available PCR kit, AMPLICOR Chlamydia trachomatis....

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Ikeda-Dantsuji Y,Konomi I,Nagayama A

    更新日期:2005-04-01 00:00:00

  • Hepatitis C virus genotyping in relation to neu-oncoprotein overexpression and the development of hepatocellular carcinoma.

    abstract::The distribution of hepatitis C virus (HCV) genotypes among Egyptian patients positive for anti-HCV was determined and their influence, when combined with neu-oncoprotein overexpression, on the development of hepatocellular carcinoma (HCC) was examined. The study groups included asymptomatic carriers (ASC) and patient...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Zekri AN,Bahnassy AA,Shaarawy SM,Mansour OA,Maduar MA,Khaled HM,El-Ahmadi O

    更新日期:2000-01-01 00:00:00

  • Comparison of two automated instruments for Epstein-Barr virus serology in a large adult hospital and implementation of an Epstein-Barr virus nuclear antigen-based testing algorithm.

    abstract::Serology remains the mainstay for diagnosis of Epstein-Barr virus (EBV) infection. This study compared two automated platforms (BioPlex 2200 and Architect i2000SR) to test three EBV serological markers: viral capsid antigen (VCA) immunoglobulins of class M (IgM), VCA immunoglobulins of class G (IgG) and EBV nuclear an...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Al Sidairi H,Binkhamis K,Jackson C,Roberts C,Heinstein C,MacDonald J,Needle R,Hatchette TF,LeBlanc JJ

    更新日期:2017-11-01 00:00:00

  • Characterisation of cell-wall-derived polypeptide antigens from different species of Mycobacterium.

    abstract::Cell walls from different species of Mycobacterium were purified on a sucrose step gradient. The components derived from these preparations were characterised by sodium dodecyl sulphate-polyacrylamide gel electrophoresis, followed by staining or by Western blotting. Surface-exposed polypeptide molecules were also iden...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Babu S,Jain NK,Prasad G,Bhattacharya S,Bhattacharya A

    更新日期:1990-08-01 00:00:00

  • Mycotic aneurysm of the abdominal aorta caused by Haemophilus influenzae type f.

    abstract::Prior to the advent of the Haemophilus influenzae type b vaccine, invasive infections due to H. influenzae type f were rarely described. However, the epidemiology of H. influenzae is changing. While the incidence of invasive infections due to H. influenzae is declining in children, such infections are becoming more co...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Suarez CJ,Glover WA,Cowan J,Smith A,Clarridge JE

    更新日期:2013-04-01 00:00:00

  • Studies on the Vibrio cholerae mucinase complex. III. Neutralisation of the neuraminidase activity by specific anti-neuraminidase IgG.

    abstract::A partially-purified neuraminidase from the mucinase complex of Vibrio cholerae was used to prepare a specific anti-neuraminidase antiserum in rabbits. When the neutralising potency of this serum against V. cholerae neuraminidase was assessed in conventional tests, the enzymic activity, as measured by thiobarbituric a...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Stewart-Tull DE,Ollar RA

    更新日期:1988-02-01 00:00:00

  • Resistance to carbapenems in sequence type 11 Klebsiella pneumoniae is related to DHA-1 and loss of OmpK35 and/or OmpK36.

    abstract::This study investigated the molecular mechanisms of carbapenem resistance in clinical isolates of Klebsiella pneumoniae from Korea and the clinical outcomes of resistant K. pneumoniae infection. Sixteen K. pneumoniae isolates showing resistance to carbapenems collected from a tertiary-care hospital were examined for t...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Shin SY,Bae IK,Kim J,Jeong SH,Yong D,Kim JM,Lee K

    更新日期:2012-02-01 00:00:00

  • Helicobacter pylori cag pathogenicity island genes: clinical relevance for peptic ulcer disease development in Brazil.

    abstract::The purpose of this study was to verify whether the presence of any of the Helicobacter pylori cagPAI genes or segments--cagA, cagA promoter, cagE, cagM, tnpB, tnpA, cagT and the left end of the cag II (LEC) region--would be a useful marker for the risk of peptic ulcer disease development. H. pylori DNA extracted from...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Mattar R,Marques SB,Monteiro MDS,Dos Santos AF,Iriya K,Carrilho FJ

    更新日期:2007-01-01 00:00:00

  • Evaluation of antigenic variations between two virulent toxoplasma strains.

    abstract::Toxoplasma gondii infection in humans is routinely assessed by serological means. Here, the authors attempted to compare the response of different Toxoplasma strains to serological tests and to evaluate the antigenic profiles of the RH and RH Ankara (TRH) strains with Western blotting. Anti-Toxoplasma IgG antibodies o...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Delibas SB,Ertabaklar H,Ertug S

    更新日期:2006-10-01 00:00:00

  • Inhibition of cell proliferation and induction of apoptosis by Helicobacter pylori through increased phosphorylated p53, p21 and Bax expression in endothelial cells.

    abstract::Microcirculation plays a crucial role in mucosal physiological function as well as repair of gastric mucosal damage. Endothelial cell damage is known to disturb microcirculation and suppress angiogenesis. Therefore, the direct effect of Helicobacter pylori on endothelial cells in vitro was investigated with H. pylori ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Kurosawa A,Miwa H,Hirose M,Tsune I,Nagahara A,Sato N

    更新日期:2002-05-01 00:00:00

  • In vitro ceftaroline combinations against meticillin-resistant Staphylococcus aureus.

    abstract::We studied in vitro ceftaroline combinations against 61 meticillin-resistant Staphylococcus aureus isolates; 18 of them were also resistant to linezolid, using overlapping E-test method. Daptomycin-ceftaroline combination obtained lower fractional inhibitory concentration values, in comparison with those including van...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: García AB,Candel FJ,López L,Chiarella F,Viñuela-Prieto JM

    更新日期:2016-10-01 00:00:00

  • Two Japanese Corynebacterium ulcerans isolates from the same hospital: ribotype, toxigenicity and serum antitoxin titre.

    abstract::Two toxigenic Corynebacterium ulcerans isolates recovered from pharyngeal swabs of two patients from the same hospital in Japan during 2001-2002 were characterized by PFGE and ribotyping. Toxin production in different culture media was examined and serological analysis of patient sera was performed. The two isolates c...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Komiya T,Seto Y,De Zoysa A,Iwaki M,Hatanaka A,Tsunoda A,Arakawa Y,Kozaki S,Takahashi M

    更新日期:2010-12-01 00:00:00

  • Inhibition of quorum-sensing-controlled virulence factors of Pseudomonas aeruginosa by Murraya koenigii essential oil: a study in a Caenorhabditis elegans infectious model.

    abstract::The global emergence of antibiotic-resistant strains of Pseudomonas aeruginosa poses a major threat in both hospital environments and the community. P. aeruginosa is an opportunistic human pathogen, and it also infects a wide range of model organisms including the nematode Caenorhabditis elegans. Quorum sensing (QS) m...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Ganesh PS,Rai RV

    更新日期:2016-12-01 00:00:00

  • Competition for glucose between Candida albicans and oral bacteria grown in mixed culture in a chemostat.

    abstract::The competition for glucose as a growth-limiting substrate between Candida albicans and a mixed community of oral bacteria was investigated. A chemostat was operated under glucose-limiting and glucose excess conditions at a dilution rate of 0.05/h. A mixed population of oral bacteria was established and after a steady...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Basson NJ

    更新日期:2000-11-01 00:00:00

  • A mechanism of pathogenicity of "Streptococcus milleri group" in pulmonary infection: synergy with an anaerobe.

    abstract::The relationship between Streptococcus constellatus, one of the species of the "Streptococcus milleri group", and Prevotella intermedia was studied in a model of pneumonia in mice and in vitro to elucidate mechanisms of pathogenicity in "S. milleri group"-associated pulmonary infection. Acute pneumonia with or without...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Shinzato T,Saito A

    更新日期:1994-02-01 00:00:00

  • Effect of inhibitors in clinical specimens on Taq and Tth DNA polymerase-based PCR amplification of influenza A virus.

    abstract::Fifteen randomly selected nasopharyngeal (NP) swab specimens (culture-negative for influenza A virus) were spiked with influenza A virus and the nucleic acids were extracted and subjected to PCR amplification with Thermus aquaticus (Taq) and T. thermophilus (Tth) DNA polymerases. Products of the expected size, and giv...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Poddar SK,Sawyer MH,Connor JD

    更新日期:1998-12-01 00:00:00

  • Modelling persistent Mycoplasma pneumoniae biofilm infections in a submerged BEAS-2B bronchial epithelial tissue culture model.

    abstract::Introduction. Infections with the respiratory pathogen Mycoplasma pneumoniae are often chronic, recurrent and resistant, persisting after antibiotic treatment. M. pneumoniae grown on glass forms protective biofilms, consistent with a role for biofilms in persistence. These biofilms consist of towers of bacteria inters...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Feng M,Burgess AC,Cuellar RR,Schwab NR,Balish MF

    更新日期:2021-01-01 00:00:00

  • Candida albicans isolates from HIV-infected and AIDS patients exhibit enhanced adherence to epithelial cells.

    abstract::The increased prevalence of oral candidosis associated with HIV infection must be intrinsically related to immunological changes in the host, but might also involve alterations to the infecting strains of yeast. This study aimed to determine if strains of Candida albicans isolated from asymptomatic HIV-infected indivi...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Sweet SP,Cookson S,Challacombe SJ

    更新日期:1995-12-01 00:00:00

  • Nasal colonization by four potential respiratory bacteria in healthy children attending kindergarten or elementary school in Seoul, Korea.

    abstract::A longitudinal analysis was carried out of the colonization by four potential respiratory pathogens - Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis and Staphylococcus aureus - in 165 healthy children (aged 3-7 years) attending three kindergartens and 417 healthy children (aged 7-10 years) att...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Bae S,Yu JY,Lee K,Lee S,Park B,Kang Y

    更新日期:2012-05-01 00:00:00

  • Population structure and antibiotic resistance of Acinetobacter DNA group 2 and 13TU isolates from hospitals in the UK.

    abstract::A total of 287 Acinetobacter isolates belonging to DNA groups 2 (A. baumannii) and 13TU was collected consecutively from 46 hospitals and typed by randomly amplified polymorphic DNA fingerprinting with primers DAF-4 and ERIC-2. With a similarity coefficient of >/=72% as a cut-off value, 37 clusters of genotypically si...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Spence RP,Towner KJ,Henwood CJ,James D,Woodford N,Livermore DM

    更新日期:2002-12-01 00:00:00

  • Rhodococcus equi can survive a phagolysosomal environment in macrophages by suppressing acidification of the phagolysosome.

    abstract::Rhodococcus equi is one of the most important causes of pneumonia in foals and has emerged as a significant opportunistic pathogen of immunosuppressed hosts such as human immunodeficiency virus-infected patients. Virulent R. equi harbouring an 85 kb plasmid, but not the avirulent form lacking the plasmid, has the abil...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Toyooka K,Takai S,Kirikae T

    更新日期:2005-11-01 00:00:00

  • Plasmid content and localisation of the STaI (STaP) gene in enterotoxigenic Escherichia coli with a non-radioactive polynucleotide gene probe.

    abstract::Enterotoxigenic Escherichia coli (ETEC) strain P2200 of porcine origin possessed eight possibly plasmid-determined characters (K88+ Raf+ Hly+ Col+ Smr Tcr Su(r) STa+) and six plasmid DNA bands of 4.2-93 kb. Analysis of the spontaneous loss of characters and the results of matings with other E. coli strains revealed th...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Bertin A

    更新日期:1992-08-01 00:00:00

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    abstract::Listeria monocytogenes cells are ingested by protozoa such as Acanthamoeba sp. or Tetrahymena pyriformis. However, they are not killed, but survive within the protozoa and may multiply intracellularly. The protozoa are lysed within about 8 days, releasing viable L. monocytogenes. No co-existence was observed between L...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章


    authors: Ly TM,Müller HE

    更新日期:1990-09-01 00:00:00