A real-time quantitative PCR assay for evaluating Clostridium difficile adherence to differentiated intestinal Caco-2 cells.

abstract：PURPOSE:We resequenced the genome of Clostridium difficile 630Δerm (DSM 28645), a model strain commonly used for the generation of insertion mutants. METHODOLOGY:The genome sequence was obtained by a combination of single-molecule real-timeand Illumina sequencing technology. RESULTS:Detailed manual curation and compa...

journal_title：Journal of medical microbiology

authors： Dannheim H,Riedel T,Neumann-Schaal M,Bunk B,Schober I,Spröer C,Chibani CM,Gronow S,Liesegang H,Overmann J,Schomburg D

更新日期：2017-03-01 00:00:00

abstract：:Cystic fibrosis (CF) patients are at great risk of opportunistic lung infection, particularly by members of the Burkholderia cepacia complex (Bcc). This group of bacteria can cause damage to the lung tissue of infected patients and are difficult to eradicate due to their high levels of antibiotic resistance. Although ...

journal_title：Journal of medical microbiology

authors： Schmerk CL,Valvano MA

更新日期：2013-02-01 00:00:00

abstract：:Since its discovery at the end of the nineteenth century, Moraxella (Branhamella) catarrhalis has undergone several changes of nomenclature and periodic changes in its perceived status as either a commensal or a pathogen. Molecular analysis based on DNA hybridisation or 16S rDNA sequence comparisons has established it...

journal_title：Journal of medical microbiology

authors： Enright MC,McKenzie H

更新日期：1997-05-01 00:00:00

abstract：:The acquisition of Klebsiella aerogenes by neonates born in hospital has been studied by sero- and klebecin typing. This organism was more commonly carried in the bowel of breast-fed babies than of bottle-fed babies. Only very rarely did babies acquire strains of K. aerogenes from their mothers. K. aerogenes was widel...

journal_title：Journal of medical microbiology

authors： Shinebaum R,Cooke EM,Brayson JC

更新日期：1979-05-01 00:00:00

abstract：:A collection of Enterococcus faecalis strains from clinical isolates, healthy individuals and the environment was screened for the presence of virulence factor genes, such as those for collagen-binding protein (ace), endocarditis antigen (efaA), haemolysin activator (cylA), gelatinase (gelE), aggregation substances (a...

journal_title：Journal of medical microbiology

authors： Creti R,Imperi M,Bertuccini L,Fabretti F,Orefici G,Di Rosa R,Baldassarri L

更新日期：2004-01-01 00:00:00

abstract：PURPOSE:Group A rotavirus (RVA) is the leading cause of severe gastroenteritis in children younger than 5 years. The most common human G-types are G1-4 and G9. G12 genotype is currently emerging worldwide, becoming the sixth most prevalent RVA G-genotype. In Tunisia, an emergence of G12 RVA strains was observed. To und...

journal_title：Journal of medical microbiology

authors： Moussa A,Fredj MBH,BenHamida-Rebaï M,Fodha I,Boujaafar N,Trabelsi A

更新日期：2017-02-01 00:00:00

abstract：:This study investigated the comparative performance of the Amplicor assay and an in-house semi-automated, multiplex real-time PCR for the diagnosis of genital chlamydial infection. Four different assays, the COBAS Amplicor CT test (Amplicor PCR), in-house real-time PCR (IHRT-PCR), in-house nested cryptic plasmid PCR a...

journal_title：Journal of medical microbiology

authors： Jalal H,Al-Suwaine A,Stephen H,Carne C,Sonnex C

更新日期：2007-03-01 00:00:00

abstract：:Electronmicrographs of sections show that the L-phases of Bacillus licheniformis var. endoparasiticus (Benedek) simulate the various types of micro-organism described by previous workers as associated with ostensibly noninfective conditions, notably cancer and arthritis; e.g., mycoplasmas, mycobacteria, corynebacteria...

journal_title：Journal of medical microbiology

authors： Bisset KA,Tallack J,Bartlett R

更新日期：1979-11-01 00:00:00

abstract：:The aim of this study was to establish bacterial profiles in gastric biopsy specimens from patients with Helicobacter pylori-associated gastritis by means of temporal temperature gradient gel electrophoresis (TTGE) of PCR-amplified 16S rDNA fragments. Specimens from eight patients with asymptomatic gastritis and five ...

journal_title：Journal of medical microbiology

authors： Monstein HJ,Tiveljung A,Kraft CH,Borch K,Jonasson J

更新日期：2000-09-01 00:00:00

abstract：:Extracts of 19 samples of pus which showed red fluorescence with ultraviolet light were screened for the presence of porphyrins by absorption spectrophotometry. All those which showed spectra typical of metal-free porphyrins were analysed by high performance liquid chromatography to identify the porphyrins present. Th...

journal_title：Journal of medical microbiology

authors： Brazier JS

更新日期：1990-09-01 00:00:00

abstract：:A cornyeform bacterium was isolated from a blood culture from a 24-year-old man with familial hypertrophic non-obstructive cardiomyopathy, chronic abuse of anabolic steroids and prior admission to hospital because of clinical signs of sepsis. 16S rRNA gene analysis unambiguously identified Gordonia terrae. ...

journal_title：Journal of medical microbiology

authors： Grisold AJ,Roll P,Hoenigl M,Feierl G,Vicenzi-Moser R,Marth E

更新日期：2007-12-01 00:00:00

abstract：:The identity and motility of enterococci isolated as urinary pathogens were compared with those of strains from other sources. The majority of isolates from all sources proved to be Streptococcus faecalis of S. faecium but eight of 117 urinary strains and seven of 127 non-urinary strains belonged to a group intermedia...

journal_title：Journal of medical microbiology

authors： Bransby RD,Grüneberg RN

更新日期：1982-02-01 00:00:00

abstract：:The closely related Clostridium novyi and Clostridium botulinum types C and D are of current interest because of their association with serious infections in injecting drug users (C. novyi type A) and equine and feline dysautonomias (C. botulinum types C/D). The species are defined by the major toxins they produce: th...

journal_title：Journal of medical microbiology

authors： Heffron A,Poxton IR

更新日期：2007-02-01 00:00:00

abstract：:Rapid diagnosis of multidrug-resistant tuberculosis (MDR-TB) is essential for the prompt initiation of effective second-line therapy to improve treatment outcome and limit transmission of this obstinate disease. A variety of molecular methods that enable the rapid detection of mutations implicated in MDR-TB have been ...

journal_title：Journal of medical microbiology

authors： Bang H,Park S,Hwang J,Jin H,Cho E,Kim DY,Song T,Shamputa IC,Via LE,Barry CE,Cho SN,Lee H

更新日期：2011-10-01 00:00:00

abstract：:The resistance of Balamuthia mandrillaris to physical, chemical and radiological conditions was tested. Following treatments, viability was determined by culturing amoebae on human brain microvascular endothelial cells for up to 12 days. B. mandrillaris cysts were resistant to repeated freeze-thawing (five times), tem...

journal_title：Journal of medical microbiology

authors： Siddiqui R,Ortega-Rivas A,Khan NA

更新日期：2008-04-01 00:00:00

abstract：PURPOSE:Multidrug-resistant Klebsiella pneumoniae is a common nosocomial pathogen that plays an important role in ventilator-associated pneumonia (VAP). This study aimed to define the clonal relatedness of K. pneumoniae strains isolated from paediatric VAP in addition to those isolated from environmental samples. METH...

journal_title：Journal of medical microbiology

authors： Mohamed ER,Aly SA,Halby HM,Ahmed SH,Zakaria AM,El-Asheer OM

更新日期：2017-05-01 00:00:00

abstract：:Enteropathogenic Escherichia coli (EPEC) is an important aetiological agent of persistent infantile diarrhoea. EPEC pathogenicity is not mediated through known toxins and the role played by outer-membrane proteins (OMPs) in the initial adherence of the bacterium, intimate attachment to epithelial cells and ultimately ...

journal_title：Journal of medical microbiology

authors： Kumar SS,Sankaran K,Haigh R,Williams PH,Balakrishnan A

更新日期：2001-07-01 00:00:00

abstract：:A novel combination of culturing and DNA-based terminal restriction fragment length polymorphism (TRFLP) analysis was used to investigate the effect of probiotics on antibiotic-induced gut microbiota alterations to determine if a probiotic preparation containing bifidobacteria and lactobacilli, taken during and after ...

journal_title：Journal of medical microbiology

authors： Engelbrektson A,Korzenik JR,Pittler A,Sanders ME,Klaenhammer TR,Leyer G,Kitts CL

更新日期：2009-05-01 00:00:00

abstract：:From 1997 to 2006, in the province of Quebec, Canada, 15 isolates of Streptococcus pseudoporcinus from 1 urine and 14 vaginorectal cultures were recovered from the genitourinary tract of pregnant women. All these women originated from the Caribbean or sub-Saharan Africa (P=0.00045 compared with a suitable control grou...

journal_title：Journal of medical microbiology

authors： Gaudreau C,Simoneau E,Labrecque O,Laurence RA,Laferrière C,Miller M,Raynal L,Rallu F

更新日期：2007-12-01 00:00:00

abstract：:The aim of this study was to assess the primary and combined resistances of Helicobacter pylori isolates obtained from paediatric patients in 2000-2001 to seven antimicrobial agents. Resistance rates of pre-treatment isolates from 115 children were investigated by the limited agar dilution method alone and by the E-te...

journal_title：Journal of medical microbiology

authors： Boyanova L,Koumanova R,Gergova G,Popova M,Mitov I,Kovacheva Y,Derejian S,Katsarov N,Nikolov R,Krastev Z

更新日期：2002-09-01 00:00:00

abstract：:A mouse enterohaemorrhagic Escherichia coli (EHEC) infection model was developed with a combination of germ-free (GF) mice and hyper-toxigenic EHEC (HT-EHEC) O157:H7 strain 6. The HT-EHEC strain 6 produced both Shiga-like toxin (SLT)-1 1.0 microg/mI and SLT-2 8.2 microg/ml in vitro. Eight-week-old germ-free mice were ...

journal_title：Journal of medical microbiology

authors： Taguchi H,Takahashi M,Yamaguchi H,Osaki T,Komatsu A,Fujioka Y,Kamiya S

更新日期：2002-04-01 00:00:00

abstract：:Human enterovirus 68 (HEV-68) is an enterovirus associated with respiratory illness. In China, no information about HEV-68 is available for children yet. This study aimed to investigate the presence of HEV-68 in mainland China between 2009 and 2012 and to explore the migration events of HEV-68 across the world. Among ...

journal_title：Journal of medical microbiology

authors： Lu QB,Wo Y,Wang HY,Wei MT,Zhang L,Yang H,Liu EM,Li TY,Zhao ZT,Liu W,Cao WC

更新日期：2014-03-01 00:00:00

abstract：:Interaction of human polymorphonuclear leucocytes (PMNL) and influenza virus (IFV) was studied in vitro. At 0 degree C, the viral particles were bound extensively to the surface of the PMNL membrane with a ratio of about 1000 virus particles to a single PMNL. The binding was sensitive to neuraminidase, suggesting atta...

journal_title：Journal of medical microbiology

authors： Yamamoto K,Suzuki K,Suzuki K,Mizuno S

更新日期：1989-03-01 00:00:00

abstract：:Introduction. Melioidosis, caused by Burkholderia pseudomallei, in endemic areas, poses a challenge for treating the diseased populations without accurate diagnosis, and the disease-specific biomarkers linked with the infection have yet to be reported. Due to the invasive nature of the causative agent, Burkholderia ps...

journal_title：Journal of medical microbiology

authors： Saikh KU,Ranji CM,Ulrich RG,Corea E,De Silva AD,Natesan M

更新日期：2020-10-01 00:00:00

abstract：:Over the last 30 years, Serratia marcescens has become an important cause of nosocomial infection. There have been many reports concerning the identification, antibiotic susceptibility, pathogenicity, epidemiological investigations and typing of this organism. Accurate identification is important in defining outbreaks...

journal_title：Journal of medical microbiology

authors： Hejazi A,Falkiner FR

更新日期：1997-11-01 00:00:00

abstract：:The effect of glucan on Leishmania major infection was studied in BALB/c mice, which are highly susceptible to leishmania infection. Glucan (0.45 mg), or isovolumetric dextrose, was administered intraperitoneally 7, 5, 3 and 1 day before infection with L. major promastigotes. At 3, 5, 6, 8 and 10 weeks after infection...

journal_title：Journal of medical microbiology

authors： Al Tuwaijri AS,Mahmoud AA,Al Mofleh IA,Al Khuwaitir SA

更新日期：1987-06-01 00:00:00

abstract：:Here, we describe four cases of laboratory-confirmed Legionella infection. Case 1 was a culture-confirmed case of Legionella infection in a patient with liver cirrhosis. Following this, three other liver cirrhosis cases (cases 2-4) were diagnosed with Legionella infection as confirmed by quantitative real-time PCR. Th...

journal_title：Journal of medical microbiology

authors： Qin T,Xia J,Ren H,Zhou H,Tang B,Shao Z

更新日期：2012-07-01 00:00:00

abstract：PURPOSE:From May 2015 to March 2016, an outbreak due to Listeria monocytogenes serotype 1/2a and clinical pulsotype never previously isolated in Europe occurred in central Italy, involving 24 confirmed clinical cases. The article provides a description of the outbreak and the investigation carried out by a multidiscipl...

journal_title：Journal of medical microbiology

authors： Duranti A,Sabbatucci M,Blasi G,Acciari VA,Ancora M,Bella A,Busani L,Centorame P,Cammà C,Conti F,De Medici D,Di Domenico M,Di Marzio V,Filippini G,Fiore A,Fisichella S,Gattuso A,Gianfranceschi M,Graziani C,Guidi F,

更新日期：2018-09-01 00:00:00

abstract：:In the present study, polyphasic analysis [cultivation, combined with the fingerprinting of individual isolates, and denaturing gradient gel electrophoresis (DGGE)] was applied to study whether similar features concerning the diversity and temporal stability of selected bacterial groups could be detected intra-individ...

journal_title：Journal of medical microbiology

authors： Maukonen J,Mättö J,Suihko ML,Saarela M

更新日期：2008-12-01 00:00:00

abstract：:Campylobacter fetus subsp. fetus infection can occur in female sheep, causing infertility or abortion. Despite extensive research on the effect of these bacteria on female fertility, little research has been done on the influence of C. fetus subsp. fetus on the male factor. Our objective was to examine the influence o...

journal_title：Journal of medical microbiology

authors： Zan Bar T,Yehuda R,Hacham T,Krupnik S,Bartoov B

更新日期：2008-11-01 00:00:00