Experimental infection of germ-free mice with hyper-toxigenic enterohaemorrhagic Escherichia coli O157:H7, strain 6.

Abstract:

:A mouse enterohaemorrhagic Escherichia coli (EHEC) infection model was developed with a combination of germ-free (GF) mice and hyper-toxigenic EHEC (HT-EHEC) O157:H7 strain 6. The HT-EHEC strain 6 produced both Shiga-like toxin (SLT)-1 1.0 microg/mI and SLT-2 8.2 microg/ml in vitro. Eight-week-old germ-free mice were inoculated intragastrically with 5.0 x 10(7) cfu of HT-EHEC strain 6. All GF mice challenged with the HT-EHEC developed ruffled fur and convulsion of limbs or hindleg weakness within 3 days after the challenge, culminating in death within 5 days. The HT-EHEC colonised well at a level of 10(8) - 10(9) cfu/g of faeces 5 days after the challenge. Both SLT-1 and SLT-2 were demonstrated in the faeces of the mice for 5 days after challenge. Histological examination of the colons of the infected mice showed congestion of the lamina propria mucosa, mild inflammatory cell infiltration and goblet cell depletion. In proximal tubules of the renal cortex, epithelial swelling with scattered necrotic cells was recognised. Endothelial swelling and mononuclear cell infiltration were also observed in the glomeruli. The brain showed acute neuronal necrosis in the cerebrum and slight loss of Purkinje cells in the cerebellum. Passive immunisation with anti-SLT antisera prolonged the life of the mice without anyneural symptoms. Microscopically, all tissue specimens from the passively immunised mice were not remarkable. These results indicate that the infection of GF mice with HT-EHEC is a useful animal model to study the pathogenicity of SLT-producing E. coliand the toxins.

journal_name

J Med Microbiol

authors

Taguchi H,Takahashi M,Yamaguchi H,Osaki T,Komatsu A,Fujioka Y,Kamiya S

doi

10.1099/0022-1317-51-4-336

keywords:

subject

Has Abstract

pub_date

2002-04-01 00:00:00

pages

336-343

issue

4

eissn

0022-2615

issn

1473-5644

journal_volume

51

pub_type

杂志文章
  • Improved serodiagnosis of Campylobacter jejuni infections using recombinant antigens.

    abstract::Campylobacter jejuni is a frequent cause of infectious diarrhoea and is increasingly recognized as a trigger for late-onset complications. The poor standardization of commonly used serological tests might explain the conflicting results regarding the frequency of antecedent C. jejuni infections in defined patient grou...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.46040-0

    authors: Schmidt-Ott R,Brass F,Scholz C,Werner C,Groß U

    更新日期:2005-08-01 00:00:00

  • Evaluation of the polymerase chain reaction for detecting the urease C gene of Helicobacter pylori in gastric biopsy samples and dental plaque.

    abstract::A polymerase chain reaction (PCR) assay with oligonucleotide primers homologous to a portion of the urease C gene of Helicobacter pylori was evaluated for specificity with pure DNA and biopsy material. The assay was used to test for the presence of the organism in dental plaque. The species specificity of detection wa...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-39-5-338

    authors: Bickley J,Owen RJ,Fraser AG,Pounder RE

    更新日期:1993-11-01 00:00:00

  • New assay for Gardnerella vaginalis loads correlates with Nugent scores and has potential in the diagnosis of bacterial vaginosis.

    abstract::Gardnerella vaginalis is a Gram-variable anaerobic bacterium present in 100% of women with bacterial vaginosis (BV). BV is a complex polymicrobial condition with no single causative agent. The current laboratory detection method for BV relies on a Gram-stain Nugent score to estimate the quantity of different bacterial...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000118

    authors: Cox C,McKenna JP,Watt AP,Coyle PV

    更新日期:2015-09-01 00:00:00

  • The use of bacteriophages to differentiate serologically cross-reactive isolates of Klebsiella pneumoniae.

    abstract::In serological typing of Klebsiella pneumoniae strains from human, equine and environmental sources, the capsular identity of many isolates could not be determined because of serological cross-reactivity. A panel of 91 bacteriophages able to lyse each of the 77 capsular serotypes of K. pneumoniae was isolated and test...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-41-6-423

    authors: Pieroni P,Rennie RP,Ziola B,Deneer HG

    更新日期:1994-12-01 00:00:00

  • Cationic currents induced by Clostridium perfringens type A enterotoxin in human intestinal CaCO-2 cells.

    abstract::Clostridium perfringens type A produces an enterotoxin that induces diarrhoea experimentally in man and animals. The enterotoxin causes increased membrane permeability in susceptible cells which is thought to be due to pore formation in the host cell membrane. The effect of purified C. perfringens enterotoxin on intac...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-48-3-235

    authors: Hardy SP,Denmead M,Parekh N,Granum PE

    更新日期:1999-03-01 00:00:00

  • Antibiotic-loaded hydroxyapatite blocks in the treatment of experimental osteomyelitis in rats.

    abstract::A novel drug delivery system for osteomyelitis was developed in which a porous hydroxyapatite block (HAB) is loaded with antibiotic by a centrifugation method. In this study, implantation of HABs loaded with the aminoglycoside antibiotic, arbekacin, were tested in established Staphylococcus aureus osteomyelitis in the...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-46-9-779

    authors: Itokazu M,Ohno T,Tanemori T,Wada E,Kato N,Watanabe K

    更新日期:1997-09-01 00:00:00

  • Role of caspases in cleavage of lamin A/C and PARP during apoptosis in macrophages infected with a periodontopathic bacterium.

    abstract::The periodontopathic bacterium Actinobacillus actinomycetemcomitans has been implicated in the pathogenesis of periodontal diseases. It has been reported previously that infection with the organism induced apoptosis in the mouse macrophage cell line J774.1. In the present study, the role of caspases during apoptosis i...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.47193-0

    authors: Okinaga T,Kasai H,Tsujisawa T,Nishihara T

    更新日期:2007-10-01 00:00:00

  • Changes in antimicrobial resistance of clinical isolates of Acinetobacter baumannii group isolated in Greece, 2010-2015.

    abstract::In recent years, hospitals in southeastern Europe have faced dramatically high rates of antibiotic-resistant Acinetobacter baumannii. We analysed the evolution of resistance among clinical isolates of A. baumannii group obtained from nine tertiary hospitals throughout Greece over 6 years (2010-2015). Identification an...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000708

    authors: Dafopoulou K,Tsakris A,Pournaras S

    更新日期:2018-04-01 00:00:00

  • Bacterial quantification in tissue homogenates from in vivo pharmacodynamic studies using growth curves.

    abstract::Introduction. Quantification of bacterial load in tissue homogenates in in vivo pharmacodynamic studies is cumbersome and time-consuming.Aim. We therefore developed a new method for quantifying bacterial load in tissue homogenates of animals treated with a β-lactam and β-lactamase inhibitor using growth curves.Methods...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.001183

    authors: Georgiou PC,Mouton JW,Pournaras S,Meletiadis J

    更新日期:2020-05-01 00:00:00

  • Langerhans cell density and serological changes following intradermal immunisation of mice with dengue 2 virus.

    abstract::After the introduction of the dengue-2 (16681) virus by intradermal (i.d.) injection into the footpads of mice, Langerhans cells (LCs) increased in numbers within 24 h at the site of injection and neutralising antibody developed. On comparing the i.d. and intramuscular (i.m.) routes, antibody was produced more rapidly...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-45-2-138

    authors: Taweechaisupapong S,Sriurairatana S,Angsubhakorn S,Yoksan S,Khin MM,Sahaphong S,Bhamarapravati N

    更新日期:1996-08-01 00:00:00

  • A Helicobacter hepaticus catalase mutant is hypersensitive to oxidative stress and suffers increased DNA damage.

    abstract::Catalase (KatA) is known to play an important role in oxidative stress resistance in many bacterial species and a homologue exists in Helicobacter hepaticus, a member of the enterohepatic Helicobacter species. Here, a katA mutant was constructed by insertional mutagenesis and its oxidative stress phenotype was investi...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.46891-0

    authors: Hong Y,Wang G,Maier RJ

    更新日期:2007-04-01 00:00:00

  • Involvement of efflux mechanisms in biocide resistance of Campylobacter jejuni and Campylobacter coli.

    abstract::Active efflux has an important role in the antimicrobial resistance of Campylobacter jejuni and Campylobacter coli. The effects of two putative efflux pump inhibitors (EPIs), phenylalanine-arginine β-naphthylamide and 1-(1-naphthylmethyl)-piperazine, and the effects of inactivation of the cmeB,cmeF and cmeR genes on r...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.041467-0

    authors: Mavri A,Smole Možina S

    更新日期:2012-06-01 00:00:00

  • Isolation of haemin-binding proteins of Neisseria gonorrhoeae.

    abstract::Although Neisseria gonorrhoeae can use haem as the sole exogenous iron source for growth in vitro, the mechanism of haem-iron uptake in the gonococcus is unknown. Two haemin-binding proteins (HmBPs) of 97 and 44 Kda were isolated by batch ligand affinity-chromatography from whole cells or total membranes of gonococci ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-36-2-121

    authors: Lee BC

    更新日期:1992-02-01 00:00:00

  • Treatment of chronic pulmonary blastomycosis with caspofungin.

    abstract::Current practice guidelines recommend that pulmonary blastomycosis be treated with antifungal agents such as amphotericin B and itraconazole. Echinocandins are not recommended because of poor in vitro activity against Blastomyces dermatitidis and lack of supporting clinical data. We report a case of chronic pulmonary ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.036103-0

    authors: Mardini J,Nguyen B,Ghannoum M,Couture C,Lavergne V

    更新日期:2011-12-01 00:00:00

  • Verocytotoxin-producing Escherichia coli (VTEC) O157 and other VTEC from human infections in England and Wales: 1995-1998.

    abstract::A total of 3429 isolations of verocytotoxin-producing Escherichia coli O157 (VTEC O157) was confirmed from human sources in England and Wales during the period 1995-1998. The largest annual total was 1087 in 1997. Most infections occurred in the third quarter of each year. The overall rate of infection ranged from 1.2...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-50-2-135

    authors: Willshaw GA,Cheasty T,Smith HR,O'Brien SJ,Adak GK

    更新日期:2001-02-01 00:00:00

  • Characterisation of target antigen of cell-mediated immunity in Mycobacterium tuberculosis H37RV.

    abstract::Antigen participating in cell-mediated immunity was isolated from a sonic extract of Mycobacterium tuberculosis H37Rv. The purified antigen had a mol. wt 150 000-200 000, based upon its Rf in 7.5% polyacrylamide gel in the presence of sodium dodecyl sulphate. In simple agarose gel electrophoresis it had an Rf of 0.85....

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-18-1-17

    authors: Hattikudur S,Kamat RS

    更新日期:1984-08-01 00:00:00

  • Characteristics of cysteine-requiring strains of Klebsiella isolated from urinary tract infections.

    abstract::Clinical and bacteriological findings in seven cases of urinary tract infection with cysteine-requiring strains of Klebsiella are described. The organisms were isolated from patients with long-standing urinary tract abnormalities and grew as small (c. 1 mm) colonies on MacConkey agar. The organisms failed to grow in a...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-26-3-211

    authors: McIver CJ,Tapsall JW

    更新日期:1988-07-01 00:00:00

  • Structural analysis of a p44/msp2 expression site of Anaplasma phagocytophilum in naturally infected ticks in Japan.

    abstract::Anaplasma phagocytophilum, an agent of human granulocytic anaplasmosis, infects neutrophils and causes an emerging tickborne febrile disease. The genome of this bacterium contains a large number of p44/msp2-related genes encoding 44 kDa major outer-membrane proteins, and it is known that a specific p44/msp2 gene is pr...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.011775-0

    authors: Wuritu,Ozawa Y,Gaowa,Kawamori F,Masuda T,Masuzawa T,Fujita H,Ohashi N

    更新日期:2009-12-01 00:00:00

  • Isolation and characterization of Siphoviridae phage infecting extensively drug-resistant Acinetobacter baumannii and evaluation of therapeutic efficacy in vitro and in vivo.

    abstract:PURPOSE:Extensively drug-resistant (XDR) strains of Acinetobacter baumannii are being reported worldwide, and they are associated with high morbidity and mortality rates. These strains are considered to be the highest priority for the development of new antibacterial agents. Therefore, we aimed to develop an effective ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.001002

    authors: Wintachai P,Naknaen A,Pomwised R,Voravuthikunchai SP,Smith DR

    更新日期:2019-07-01 00:00:00

  • Influence of capsule size on the in vitro activity of antifungal agents against clinical Cryptococcus neoformans var. grubii strains.

    abstract::Cryptococcosis causes disseminated disease in AIDS patients. In contrast to what occurs in laboratory conditions, a large capsule is produced by Cryptococcus neoformans in vivo during infection. The aim of this study was to compare the in vitro activity of different antifungal agents against 34 clinical isolates of C....

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.036152-0

    authors: Vitale RG,Pascuccelli V,Afeltra J

    更新日期:2012-03-01 00:00:00

  • Identifying indoor air Penicillium species: a challenge for allergic patients.

    abstract:PURPOSE:Penicillium is the most common mould isolated in housing. Penicillium chrysogenum is the only species tested by prick test or serology for allergic patients. The American Institute of Medicine has accepted Penicillium as an aetiological agent of rhinitis in children and adults and as an asthma agent in children...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000960

    authors: Reboux G,Rocchi S,Vacheyrou M,Millon L

    更新日期:2019-05-01 00:00:00

  • Comparison of enterotoxins and haemolysins produced by methicillin-resistant (MRSA) and sensitive (MSSA) Staphylococcus aureus.

    abstract::A collection of 201 isolates of Staphylococcus aureus was examined: 152 methicillin-sensitive S. aureus (MSSA) comprised 48 blood culture isolates (BC) and 58 isolates from routine diagnostic specimens (RD) from Glasgow Royal Infirmary (GRI), and 46 strains from nasal swabs of patients attending a general practitioner...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-36-3-164

    authors: Coia JE,Browning L,Haines L,Birkbeck TH,Platt DJ

    更新日期:1992-03-01 00:00:00

  • Prevalent genotypes of meticillin-resistant Staphylococcus aureus: report from Pakistan.

    abstract::Meticillin-resistant Staphylococcus aureus (MRSA) is a major nosocomial pathogen in Pakistan and is emerging in the community. This is one of the first reports of the prevalent genotypes of MRSA in both hospital and community settings in Pakistan. Isolates collected in 2006-2007 were characterized by PFGE, staphylococ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.022707-0

    authors: Zafar A,Stone M,Ibrahim S,Parveen Z,Hasan Z,Khan E,Hasan R,Wain J,Bamford K

    更新日期:2011-01-01 00:00:00

  • Application of terminal RFLP analysis to characterize oral bacterial flora in saliva of healthy subjects and patients with periodontitis.

    abstract::Terminal restriction fragment-length polymorphism (T-RFLP) analysis was applied to characterize oral bacterial flora in saliva from 18 healthy subjects and 18 patients with periodontitis. The 16S rRNA genes (rDNAs) of oral bacteria and spirochaetes in saliva were amplified by PCR with a 6'carboxy-fluorescein (6-FAM)-l...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.04991-0

    authors: Sakamoto M,Takeuchi Y,Umeda M,Ishikawa I,Benno Y

    更新日期:2003-01-01 00:00:00

  • Hospital clonal dissemination of Enterobacter aerogenes producing carbapenemase KPC-2 in a Chinese teaching hospital.

    abstract::Carbapenems are first-line agents for the treatment of serious nosocomial infections caused by multidrug-resistant Enterobacteriaceae. However, resistance to carbapenems has increased dramatically among Enterobacteriaceae in our hospital. In this study, we report clonal dissemination caused by carbapenem-resistant Ent...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.064865-0

    authors: Qin X,Yang Y,Hu F,Zhu D

    更新日期:2014-02-01 00:00:00

  • Evaluation of a newly developed down-flow immunoassay for detection of serum mannan antigens in patients with candidaemia.

    abstract::A down-flow immunoassay has been developed to detect serum mannan antigens, and the test was recently marketed as the Unimedi Candida monotest. Using 251 serum samples from 105 patients with candidaemia, a comparison of the Unimedi Candida monotest with the Cand-Tec latex agglutination test and 2 microplate enzyme imm...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.46314-0

    authors: Fujita SI,Takamura T,Nagahara M,Hashimoto T

    更新日期:2006-05-01 00:00:00

  • Re-evaluation of rejection criteria for endotracheal tube (ETT) specimens from adult patients.

    abstract::The purpose of this study was to determine optimal criteria for microbiology laboratory screening of endotracheal tube (ETT) specimens submitted for bacterial culture from adult patients. ETT specimens from adult patients that were received by two microbiology laboratories were prospectively evaluated and subdivided i...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.042333-0

    authors: Walkty A,Lagacé-Wiens PRS,Manickam K,Adam H,Pieroni P,Alfa M,Karlowsky JA

    更新日期:2012-09-01 00:00:00

  • Reduced susceptibility to carbapenems in Klebsiella pneumoniae clinical isolates associated with plasmid-mediated beta-lactamase production and OmpK36 porin deficiency.

    abstract::Two carbapenem-non-susceptible Klebsiella pneumoniae isolates, Z2554 and Z2110, were collected from a hospital in China and analysed by PFGE. K. pneumoniae Z2554 and Z2110 were genetically unrelated and showed resistance to ertapenem, and reduced susceptibility to imipenem and meropenem. Analysis of their beta-lactama...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.008094-0

    authors: Wang XD,Cai JC,Zhou HW,Zhang R,Chen GX

    更新日期:2009-09-01 00:00:00

  • Inhibition of streptolysin O by allicin - an active component of garlic.

    abstract::Streptolysin O (SLO) is a potent cytolytic toxin produced by almost all strains of group A streptococci and is considered an important virulence factor for this organism. In this study we investigated the effect of allicin and aqueous garlic extracts on the haemolytic activity of SLO. All tested materials potentially ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.019539-0

    authors: Arzanlou M,Bohlooli S

    更新日期:2010-09-01 00:00:00

  • Protection of mice against vaginal colonisation by Mycoplasma pulmonis.

    abstract::Resistance against vaginal colonisation by Mycoplasma pulmonis in strain TO mice after exposure to the mycoplasma was investigated. Eighteen mice from which M. pulmonis had been eliminated from the vagina, either naturally or by antibiotic therapy, were resistant to vaginal recolonisation. Specific antibody was measur...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-40-3-197

    authors: Taylor-Robinson D,Furr PM

    更新日期:1994-03-01 00:00:00