Genome sequence analysis of emm89 Streptococcus pyogenes strains causing infections in Scotland, 2010-2016.

abstract：:Using a Clostridium difficile glutamate dehydrogenase (GDH) immunoassay and a sensitive C. difficile toxin A/B immunoassay, human stool specimens from patients with diarrhoea (n = 1085) were classified as either GDH positive/toxin negative, or GDH positive/toxin positive. Overall, 528/725 (73%) of the GDH-positive/tox...

journal_title：Journal of medical microbiology

authors： Fairley DJ,McKenna JP,Stevenson M,Weaver J,Gilliland C,Watt A,Coyle PV

更新日期：2015-11-01 00:00:00

abstract：:Biofilms are colonies of microbial cells encased in a self-produced organic polymeric matrix and represent a common mode of microbial growth. Microbes growing as biofilm are highly resistant to commonly used antimicrobial drugs. We aimed to screen and characterize biofilm formation by different isolates of Candida on ...

journal_title：Journal of medical microbiology

authors： Zahran KM,Agban MN,Ahmed SH,Hassan EA,Sabet MA

更新日期：2015-04-01 00:00:00

abstract：:A monospecific polyclonal antiserum, prepared against Bacteroides fragilis common polysaccharide antigen purified by polyacrylamide gel immunoblot detected B. fragilis, B. thetaiotaomicron, B. ovatus and Prevotella melaninogenica in pus samples from various anatomical sites by immunofluorescence microscopy of the pus....

journal_title：Journal of medical microbiology

authors： Patrick S,Stewart LD,Damani N,Wilson KG,Lutton DA,Larkin MJ,Poxton I,Brown R

更新日期：1995-08-01 00:00:00

abstract：:Escherichia coli (2), Vibrio cholerae (2) and Aeromonas sobria (1) strains were examined for their ability to grow and produce toxins in samples of ileostomy fluid. Three categories of response were observed: no detectable growth, growth without detectable toxin, and growth with detectable toxin. Clear differences wer...

journal_title：Journal of medical microbiology

authors： Ala Aldeen DA,Barer MR

更新日期：1989-04-01 00:00:00

abstract：:Diagnosis of human immunodeficiency virus (HIV) infection by antibody-based testing allows for some recently infected individuals to be falsely assessed as non-infected. Since such individuals often have high viral loads and are capable of transmitting HIV, it is an imperative public health need to identify these indi...

journal_title：Journal of medical microbiology

authors： Ren A,Louie B,Rauch L,Castro L,Liska S,Klausner JD,Pandori MW

更新日期：2008-10-01 00:00:00

abstract：:Prevotella intermedia and the newly described P. nigrescens cannot be reliably distinguished by phenotypic tests. In this study, restriction endonuclease digestion of amplified 16S rDNA (16S rDNA PCR-RFLP) was used to generate restriction profiles of the type strains of P. intermedia and P. nigrescens and 43 fresh iso...

journal_title：Journal of medical microbiology

authors： Milsom SE,Sprague SV,Dymock D,Weightman AJ,Wade WG

更新日期：1996-01-01 00:00:00

abstract：:Helicobacter pylori infection represents a key factor in the aetiology of various gastrointestinal diseases. H. pylori infection diagnosis is generally achieved using both invasive (e.g. biopsy of the gastric epithelium) and non-invasive methods. Therefore, cultivation on a growth medium becomes complex. Trypsin is a ...

journal_title：Journal of medical microbiology

authors： Peretz A,Paritsky M,Pastukh N,Koifman A,Brodsky D,Glyatman T,On A

更新日期：2015-06-01 00:00:00

abstract：PURPOSE:Pertussis remains a public health concern in most countries. Our study aimed to prospectively explore the epidemiology of pertussis in the Tunis area of Tunisia between 2007 and 2016, and to characterize the virulence-associated genes of the collected Bordetella pertussis isolates. METHODOLOGY:Infants and chil...

journal_title：Journal of medical microbiology

authors： Ben Fraj I,Kechrid A,Guillot S,Bouchez V,Brisse S,Guiso N,Smaoui H

更新日期：2019-02-01 00:00:00

abstract：:Staphylococcus aureus produces numerous bi-component toxins, e.g., Panton-Valentine leukocidin (Luk-PVL) and gamma-haemolysin, which consist of type S and F proteins. Previous studies showed that Luk-PVL induces inflammatory mediator release from human granulocytes that might reflect the in-vivo effects, e.g., dermone...

journal_title：Journal of medical microbiology

authors： König B,Prévost G,König W

更新日期：1997-06-01 00:00:00

abstract：:Early diagnosis of leptospirosis is important because severe leptospiral infection can run a fulminant course. The polymerase chain reaction (PCR) was evaluated for the detection of leptospires in clinical samples from patients with acute leptospiral infection. Blood and urine samples from 71 patients with leptospiros...

journal_title：Journal of medical microbiology

authors： Brown PD,Gravekamp C,Carrington DG,van de Kemp H,Hartskeerl RA,Edwards CN,Everard CO,Terpstra WJ,Levett PN

更新日期：1995-08-01 00:00:00

abstract：:The cell-wall characteristics of Gardnerella vaginalis grown in conventional and biofilm systems were studied by electron microscopy. The gram-positive nature of the cell wall was confirmed. Novel cell-wall particles which appeared to be associated with cell division were also identified, particularly in organisms of ...

journal_title：Journal of medical microbiology

authors： Muli FW,Struthers JK,Tarpey PA

更新日期：1999-02-01 00:00:00

abstract：:Yersinia pseudotuberculosis was examined for its haemagglutinating activity and adherence to cultured epithelial cells (HEp-2) in relation to possession of a virulence (VW) plasmid and to growth conditions. VW-lacking (VW-) bacteria were isolated from ten VW+ strains of each serovar which, after they were grown on CFA...

journal_title：Journal of medical microbiology

authors： Kawaoka Y,Mitani T,Hoshina K,Otsuki K,Tsubokura M

更新日期：1988-03-01 00:00:00

abstract：:A collection of Enterococcus faecalis strains from clinical isolates, healthy individuals and the environment was screened for the presence of virulence factor genes, such as those for collagen-binding protein (ace), endocarditis antigen (efaA), haemolysin activator (cylA), gelatinase (gelE), aggregation substances (a...

journal_title：Journal of medical microbiology

authors： Creti R,Imperi M,Bertuccini L,Fabretti F,Orefici G,Di Rosa R,Baldassarri L

更新日期：2004-01-01 00:00:00

abstract：:The relationship between Streptococcus constellatus, one of the species of the "Streptococcus milleri group", and Prevotella intermedia was studied in a model of pneumonia in mice and in vitro to elucidate mechanisms of pathogenicity in "S. milleri group"-associated pulmonary infection. Acute pneumonia with or without...

journal_title：Journal of medical microbiology

authors： Shinzato T,Saito A

更新日期：1994-02-01 00:00:00

abstract：:In the present study, the adhesion of bacteriocin-producing probiotic strains of Lactobacillus plantarum onto extracellular matrix (ECM) proteins such as collagen and mucin and their potential to prevent pathogen invasion onto the ECM was ascertained. Fluorescence-based in vitro assays indicated that L. plantarum stra...

journal_title：Journal of medical microbiology

authors： Mukherjee S,Ramesh A

更新日期：2015-12-01 00:00:00

abstract：PURPOSE:This study aimed to explore daptomycin combined with fosfomycin or rifampin against the planktonic and adherent linezolid-resistant isolates of Enterococcus faecalis. METHODOLOGY:Four linezolid-resistant and four linezolid-sensitive isolates of E. faecalis which formed biofilms were collected for this study. B...

journal_title：Journal of medical microbiology

authors： Zheng JX,Sun X,Lin ZW,Qi GB,Tu HP,Wu Y,Jiang SB,Chen Z,Deng QW,Qu D,Yu ZJ

更新日期：2019-03-01 00:00:00

abstract：:Microcirculation plays a crucial role in mucosal physiological function as well as repair of gastric mucosal damage. Endothelial cell damage is known to disturb microcirculation and suppress angiogenesis. Therefore, the direct effect of Helicobacter pylori on endothelial cells in vitro was investigated with H. pylori ...

journal_title：Journal of medical microbiology

authors： Kurosawa A,Miwa H,Hirose M,Tsune I,Nagahara A,Sato N

更新日期：2002-05-01 00:00:00

abstract：:Small numbers of bacteria capable of growing on agar supplemented with amoxycillin 40 mg/L were isolated from the saliva of 9 out of 20 adult volunteers in a previous study. All the bacteria were identified as Streptococcus sanguis although no strains produced dextran in conventional tests. However, using a specific a...

journal_title：Journal of medical microbiology

authors： Marsh PD,West AA,Keevil CW

更新日期：1987-11-01 00:00:00

abstract：:An ELISA was developed and evaluated as a method for detecting antibodies against glycosylated and deglycosylated histoplasmin (HMIN). Sera from patients with histoplasmosis, paracoccidioidomycosis, sporotrichosis, coccidioidomycosis, aspergillosis, cryptococcosis and healthy donors were tested by ELISA against purifi...

journal_title：Journal of medical microbiology

authors： Guimarães AJ,Pizzini CV,de Matos Guedes HL,Albuquerque PC,Peralta JM,Hamilton AJ,Zancopé-Oliveira RM

更新日期：2004-06-01 00:00:00

abstract：:The antifungal effects of cinnamaldehyde, 4-hydroxy-3-methoxycinnamaldehyde (coniferyl aldehyde) and 3,5-dimethoxy-4-hydroxycinnamaldehyde (sinapaldehyde) were investigated against 65 strains of Candida (six standard, 39 fluconazole-sensitive and 20 fluconazole-resistant). MICs of cinnamaldehyde, coniferyl aldehyde an...

journal_title：Journal of medical microbiology

authors： Shreaz S,Bhatia R,Khan N,Muralidhar S,Manzoor N,Khan LA

更新日期：2013-02-01 00:00:00

abstract：PURPOSE:Urinary tract infection (UTI) can be community-acquired (Com-UTI) or catheter-associated (CAUTI) and may be associated with biofilm-producing organisms. A comparative analysis of biofilm-producing property (BPP), antibiotic-sensitivity and multi-drug resistance (MDR) and their relation with the BPP of isolates ...

journal_title：Journal of medical microbiology

authors： Bardoloi V,Yogeesha Babu KV

更新日期：2017-07-01 00:00:00

abstract：:In recent years, hospitals in southeastern Europe have faced dramatically high rates of antibiotic-resistant Acinetobacter baumannii. We analysed the evolution of resistance among clinical isolates of A. baumannii group obtained from nine tertiary hospitals throughout Greece over 6 years (2010-2015). Identification an...

journal_title：Journal of medical microbiology

authors： Dafopoulou K,Tsakris A,Pournaras S

更新日期：2018-04-01 00:00:00

abstract：:Accurate susceptibility results on antibiotic-resistant bacteria are essential for proper treatment of infections. In this study, 100 metallo-β-lactamase (MBL)-producing strains and 95 isolates with Klebsiella pneumoniae carbapenemase (KPC) were tested for carbapenem susceptibility using two automated platforms, the P...

journal_title：Journal of medical microbiology

authors： Sun J,Xu Y,Yu Y,Ni Y

更新日期：2015-06-01 00:00:00

abstract：:The family of leptospiral immunoglobulin-like (lig) genes comprises ligA, ligB and ligC. This study used PCR to demonstrate the presence of lig genes among serovars from a collection of leptospiral strains and clinical isolates. Whilst ligA and ligC appeared to be present in a limited number of pathogenic serovars, th...

journal_title：Journal of medical microbiology

authors： Cerqueira GM,McBride AJA,Picardeau M,Ribeiro SG,Moreira ÂN,Morel V,Reis MG,Ko AI,Dellagostin OA

更新日期：2009-09-01 00:00:00

abstract：:In a prospective study between February 2003 and June 2004, stool specimens of children less than 2 years of age with diarrhoea (n=218) and without diarrhoea (n=86), living in Vitória, Espírito Santo, Brazil, were examined for the presence of diarrhoeagenic Escherichia coli. E. coli isolates were tested by colony blot...

journal_title：Journal of medical microbiology

authors： Spano LC,Sadovsky ADI,Segui PN,Saick KW,Kitagawa SMS,Pereira FEL,Fagundes-Neto U,Scaletsky ICA

更新日期：2008-03-01 00:00:00

abstract：:Shiga toxins (Stx1 and Stx2) are responsible for initiating haemolytic uraemic syndrome, a serious extraintestinal complication caused by enterohaemorrhagic Escherichia coli O157 : H7 infection in humans. Shiga toxins are classical AB(5)-type exotoxins, consisting of a globotriaosylceramide (Gb(3))-binding B subunit p...

journal_title：Journal of medical microbiology

authors： Griener TP,Mulvey GL,Marcato P,Armstrong GD

更新日期：2007-11-01 00:00:00

abstract：:In the present study, polyphasic analysis [cultivation, combined with the fingerprinting of individual isolates, and denaturing gradient gel electrophoresis (DGGE)] was applied to study whether similar features concerning the diversity and temporal stability of selected bacterial groups could be detected intra-individ...

journal_title：Journal of medical microbiology

authors： Maukonen J,Mättö J,Suihko ML,Saarela M

更新日期：2008-12-01 00:00:00

abstract：PURPOSE:We examined evidence for transmission of Pandorea apista among cystic fibrosis (CF) patients attending paediatric and adult services in one city who had previously been found to harbour related isolates by pulsed-field gel electrophoresis (PFGE). METHODOLOGY:The whole-genome sequences of 18 isolates from this ...

journal_title：Journal of medical microbiology

authors： Kenna DTD,Coward A,Perry C,Pike R,Schaefer U,Turton J,Green H,Jones AM,Bright-Thomas RJ,Burns P,Narayan O,Wilkinson S,Turton JF

更新日期：2019-07-01 00:00:00

abstract：:Thirty-seven multidrug-resistant and 13 pan-susceptible isolates of Mycobacterium tuberculosis were analysed for the diversity of genotypes associated with known drug-resistance mechanisms. The isolates were obtained from patients attending a university tuberculosis clinic in Monterrey, Mexico. A total of 25 IS6110-RF...

journal_title：Journal of medical microbiology

authors： Ramaswamy SV,Dou SJ,Rendon A,Yang Z,Cave MD,Graviss EA

更新日期：2004-02-01 00:00:00

abstract：:Of 200 isolates of Vibrio mimicus screened, one from water (N-57) agglutinated with V. cholerae O139 polyclonal antiserum (absorbed with a rough strain of V. cholerae only) and not with O139 polyclonal diagnostic antiserum (absorbed with the rough strain and V. cholerae O22 and O155). The antigenic relationship betwee...

journal_title：Journal of medical microbiology

authors： Ansaruzzaman M,Shimada T,Bhuiyan NA,Nahar S,Alam K,Islam MS,Albert MJ

更新日期：1999-09-01 00:00:00