Molecular analysis and species diversity of Nocardia in the hospital environment in a developing country, a potential health hazard.

Abstract:

PURPOSE:Despite hundreds of reports on the isolation of Nocardia from clinical samples, the presence and diversity of Nocardia species that are capable of survival in a harsh and adverse condition, such as a hospital environment, have not been comprehensively studied. The aim of this study was to assess Nocardia species diversity in a hospital environment to provide a better insight into their potential threat as a reservoir for the development of nosocomial infections. METHODOLOGY:A total of 90 samples of hospital water, dust and soil, collected from 30 hospitals, were analysed for the presence of Nocardia using standard protocols for isolation and characterization of the isolates. Conventional tests were used for preliminary identification, and PCR amplification of the 596 bp amplicon of the 16S rRNA and sequence analysis of 16S rRNA were performed for genus and species identification. RESULTS:A total of 25 Nocardia isolates (27.7 %) from 10 species were recovered from 90 samples. The three most prevalent species were N. cyriacigeorgica, 24 %, N. asteroides, 16 % and N. kroppenstedtii, 12 %, followed by N. salmonicida-like, 8 % and single isolates of N.otitidiscaviarum, N. flavorozea-like, N. neocaledoniensis-like and N. sungurluensis-like. Thirteen out of twenty five isolates showed characteristics of six novel species. CONCLUSION:Our study showed that the hospital environment is a potential reservoir of a diverse range of Nocardia species, due to the remarkable survival capability of these bacteria in an adverse hospital environment, which carries a threat to the health of patients.

journal_name

J Med Microbiol

authors

Rahdar HA,Azadi D,Shojaei H,Daei-Naser A

doi

10.1099/jmm.0.000436

subject

Has Abstract

pub_date

2017-03-01 00:00:00

pages

334-341

issue

3

eissn

0022-2615

issn

1473-5644

journal_volume

66

pub_type

杂志文章
  • A simple statistical approach that represents the frequency distribution of plasmids in clinical isolates of the enterobacteria.

    abstract::The frequency distribution of plasmids in a representative collection of Escherichia coli and other enterobacteria was compared with the frequencies predicted by the Poisson distribution. The distribution of E. coli plasmids did not differ significantly (p greater than 0.2) whereas the difference between observed and ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-23-3-255

    authors: Platt DJ

    更新日期:1987-05-01 00:00:00

  • Candida auris: a comparison between planktonic and biofilm susceptibility to antifungal drugs.

    abstract::Introduction.Candida auris is a pathogenic yeast that mainly affects immunosuppressed patients and those with implanted medical devices. This pathogen also displays elevated resistance to common antifungals and high survival and spreading capacities. Since no antifungal breakpoints have yet been defined for this patho...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.001036

    authors: Romera D,Aguilera-Correa JJ,Gadea I,Viñuela-Sandoval L,García-Rodríguez J,Esteban J

    更新日期:2019-09-01 00:00:00

  • Studies on early association of Salmonella typhimurium with intestinal mucosa in vivo and in vitro: relationship to virulence.

    abstract::The abilities of six strains of Salmonella typhimurium to associate with rabbit ileal mucosa have been measured in vitro. Two were "virulent" strains (TML and W118 which are invasive and inducers of fluid secretion in rabbit ileal loops); four were "avirulent" (LT7, M206 and SL1027 which are invasive but induce neglig...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-29-4-283

    authors: Worton KJ,Candy DC,Wallis TS,Clarke GJ,Osborne MP,Haddon SJ,Stephen J

    更新日期:1989-08-01 00:00:00

  • A paradigm for the molecular identification of Mycobacterium species in a routine diagnostic laboratory.

    abstract::The aim of this study was to improve the identification of Mycobacterium species in the context of a UK teaching hospital. Real-time PCR assays were established to enable the rapid differentiation between Mycobacterium tuberculosis (MTB) complex and Mycobacterium species other than tuberculosis (MOTT), followed by 16S...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.46855-0

    authors: Williams KJ,Ling CL,Jenkins C,Gillespie SH,McHugh TD

    更新日期:2007-05-01 00:00:00

  • Group C Streptococcus dysgalactiae subsp. equisimilis in south-east Brazil: genetic diversity, resistance profile and the first report of human and equine isolates belonging to the same multilocus sequence typing lineage.

    abstract::Streptococcus dysgalactiae subsp. equisimilis (SDSE) isolates are the most common group C streptococci in humans and reports of invasive infections associated with SDSE have been increasing. Molecular epidemiology studies are an important strategy to trace the emergence and spread of possible well-fit bacterial pathog...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000052

    authors: Silva LG,Genteluci GL,Corrêa de Mattos M,Glatthardt T,Sá Figueiredo AM,Ferreira-Carvalho BT

    更新日期:2015-05-01 00:00:00

  • Characterisation of methicillin-resistant Staphylococcus aureus by biotyping, immunoblotting and restriction enzyme fragmentation patterns.

    abstract::We have characterised 45 isolates of methicillin-resistant Staphylococcus aureus (MRSA) from Glasgow Royal Infirmary by means of simple biotyping, immunoblotting of exported proteins and restriction enzyme fragmentation patterns (REFP) of plasmid DNA. The strains were subdivided into four groups (A-D) on the basis of ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-31-2-125

    authors: Coia JE,Thomson-Carter F,Baird D,Platt DJ

    更新日期:1990-02-01 00:00:00

  • The isolation of Streptococcus pyogenes from throat swabs.

    abstract::Simulated throat swabs were prepared with known numbers of Streptococcus pyogenes; some were suspended in pasteurised human saliva and an equal number in saline. Two types of commercially available swabs were tested; these were composed of (1) plain, buffered, cotton wool, and (2) albumen-coated cotton wool. The mean ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-10-1-69

    authors: Ross PW

    更新日期:1977-02-01 00:00:00

  • Cross-reactions in cell-mediated immunity to Salmonella causing enteric fever.

    abstract::Cross-reactivity in a delayed-type hypersensitivity (DH) response was studied in mice immunised with live Salmonella typhi, S. paratyphi A and S. paratyphi B. Extensive cross-reactions outside the serogroup limits were observed. The ability of DH cross-reacting and non-cross-reacting sonicates to generate activated ma...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-21-3-233

    authors: Tiwari H,Kamat RS

    更新日期:1986-05-01 00:00:00

  • Immunogenicity of mycobacterial PPE44 (Rv2770c) in Mycobacterium bovis BCG-infected mice.

    abstract::The Pro-Pro-Glu (PPE) protein family of Mycobacterium tuberculosis includes 69 glycine-rich proteins with a conserved N-terminal domain. Their role in tuberculosis is unknown, but it has been speculated that they may have an important immunological significance. In this investigation, the immunogenicity of the ppe44 (...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.45960-0

    authors: Bonanni D,Rindi L,Lari N,Garzelli C

    更新日期:2005-05-01 00:00:00

  • A simplified system for the identification of staphylococci by multipoint inoculation of test media.

    abstract::Most hospital bacteriologists have divided staphylococci into two groups: Staphylococcus aureus and the coagulase-negative staphylococci of which the novobiocin-resistant varieties are termed S. saprophyticus. The identification of S. aureus has been easy but that of the other staphylococci has provided some difficult...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-22-2-179

    authors: Reuther JW

    更新日期:1986-09-01 00:00:00

  • The isolation and cloning of chromosomal DNA specific for a clonal population of Staphylococcus aureus by subtractive hybridisation.

    abstract::Subtractive hybridisation was used to screen for and identify conserved DNA sequences associated with clinical, clonal populations of Staphylococcus aureus. DNA from S. aureus strain ISP8 (a clinical isolate) was digested with TaqI and hybridised with randomly fragmented pooled DNA from 10 non-clinical (community) iso...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-46-12-987

    authors: el-Adhami WA,Stewart PR,Matthaei KI

    更新日期:1997-12-01 00:00:00

  • Continuous culture of sessile human oropharyngeal microbiotas.

    abstract::The microbiota of the human oropharynx plays an important role in health through involvement in the aetiology of infection and the carriage of adventitious pathogens. Despite this, there are few models available for the preclinical assessment of novel antimicrobials directed to the human throat. We have profiled bacte...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.055806-0

    authors: Humphreys GJ,McBain AJ

    更新日期:2013-06-01 00:00:00

  • Development of a multiplex-PCR for direct detection of the genes for enterotoxin B and C, and toxic shock syndrome toxin-1 in Staphylococcus aureus isolates.

    abstract::As well as conventional methods such as immunodiffusion, ELISA, or agglutination for the detection of toxin production in Staphylococcus aureus, amplification techniques like PCR allow a very sensitive and specific identification of the genes responsible for enterotoxin B and C, and TSST-1 production. These toxins mig...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-47-4-335

    authors: Schmitz FJ,Steiert M,Hofmann B,Verhoef J,Hadding U,Heinz HP,Köhrer K

    更新日期:1998-04-01 00:00:00

  • Evaluation of CHROM-Pal medium for the isolation and direct identification of Candida dubliniensis in primary cultures from the oral cavity.

    abstract::Candida albicans is the species most frequently isolated from oral specimens, but the recovery of other Candida species such as Candida dubliniensis is increasing. Differentiation of C. dubliniensis from C. albicans requires special tests and both species are misidentified in some studies. CHROM-Pal (CH-P) is a novel ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.011320-0

    authors: Sahand IH,Maza JL,Eraso E,Montejo M,Moragues MD,Aguirre JM,Quindós G,Pontón J

    更新日期:2009-11-01 00:00:00

  • Coxsackie B virus-specific IgM antibody and myocardial infarction.

    abstract::The ELISA technique was shown to be group-specific for the detection of IgM antibodies against coxsackie B viruses, and probably against a wider range of enteroviruses. No evidence was obtained that recent coxsackie B-virus infection predisposes to myocardial infarction. ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-21-4-287

    authors: Hannington G,Booth JC,Bowes RJ,Stern H

    更新日期:1986-06-01 00:00:00

  • An increase in p62/NBR1 levels in melioidosis patients of Sri Lanka exhibit a characteristic of potential host biomarker.

    abstract::Introduction. Melioidosis, caused by Burkholderia pseudomallei, in endemic areas, poses a challenge for treating the diseased populations without accurate diagnosis, and the disease-specific biomarkers linked with the infection have yet to be reported. Due to the invasive nature of the causative agent, Burkholderia ps...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.001242

    authors: Saikh KU,Ranji CM,Ulrich RG,Corea E,De Silva AD,Natesan M

    更新日期:2020-10-01 00:00:00

  • Alteration of pulmonary structure by Pseudomonas aeruginosa exoenzyme S.

    abstract::Intratracheal administration of purified Pseudomonas aeruginosa exoenzyme S elicited extensive, grossly observable damage in the rat lung within 2 h. Light and electronmicroscopy revealed injury and necrosis of bronchial epithelium, type I pneumocytes and capillary endothelial cells after 1 h; associated haemorrhage, ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-26-2-133

    authors: Woods DE,Hwang WS,Shahrabadi MS,Que JU

    更新日期:1988-06-01 00:00:00

  • Identification of mutants with altered phenazine production in Pseudomonas aeruginosa.

    abstract::Pseudomonas aeruginosa is an opportunistic human pathogen that causes serious and chronic infections. Many secondary metabolites are secreted throughout its growth, among which phenazine is a known virulence factor and signalling molecule. Phenazine is coordinately controlled by the global regulatory quorum-sensing (Q...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.022350-0

    authors: Liang H,Duan J,Sibley CD,Surette MG,Duan K

    更新日期:2011-01-01 00:00:00

  • Bacteroides fragilis is a silent pathogen in acute appendicitis.

    abstract::The role of Bacteroides fragilis in the pathogenesis of acute appendicitis was studied in 135 patients in four patient groups: normal (17); phlegmonous appendicitis (17); gangrenous appendicitis (75); and septic complications of appendicitis (26). Aerobic and anaerobic bacteria were isolated from all groups and member...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-21-3-245

    authors: Elhag KM,Alwan MH,Al-Adnani MS,Sherif RA

    更新日期:1986-05-01 00:00:00

  • Improvement and optimization of the classical gastric biopsy culture technique for Helicobacter pylori diagnosis using trypsin.

    abstract::Helicobacter pylori infection represents a key factor in the aetiology of various gastrointestinal diseases. H. pylori infection diagnosis is generally achieved using both invasive (e.g. biopsy of the gastric epithelium) and non-invasive methods. Therefore, cultivation on a growth medium becomes complex. Trypsin is a ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000054

    authors: Peretz A,Paritsky M,Pastukh N,Koifman A,Brodsky D,Glyatman T,On A

    更新日期:2015-06-01 00:00:00

  • Evaluation of internal transcribed spacer 2-RFLP analysis for the identification of dermatophytes.

    abstract::A total of 95 isolates, belonging to 33 species of five dermatophyte genera, i.e. Arthroderma (15 species), Chrysosporium (two), Epidermophyton (one), Microsporum (three) and Trichophyton (12), were studied using internal transcribed spacer 2 (ITS2)-PCR-RFLP analysis (ITS2-RFLP), consisting of amplification of the ITS...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.013870-0

    authors: De Baere T,Summerbell R,Theelen B,Boekhout T,Vaneechoutte M

    更新日期:2010-01-01 00:00:00

  • Distribution and transferability of plasmids in trimethoprim resistant urinary Escherichia coli.

    abstract::Urinary isolates of Escherichia coli that were resistant to trimethoprim were collected in Glasgow Royal Infirmary during 1979 and 1980. Eighty-eight were resistant to trimethoprim 1024 micrograms/ml and 80 (92%) were also resistant to sulphamethoxazole 1024 micrograms/ml; 73% were multiresistant. Plasmids were detect...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-16-4-433

    authors: Kraft CA,Platt DJ,Timbury MC

    更新日期:1983-11-01 00:00:00

  • Strain, clone and species: comments on three basic concepts of bacteriology.

    abstract::Different aspects of the terms strain, clone and species are discussed. The term strain is commonly used to denote a pure culture - here called 'the strain in the taxonomic sense' - but does also refer to a natural concept closely related to the clone. The term clone on the other hand is used both in a general and in ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章,评审

    doi:10.1099/0022-1317-49-5-397

    authors: Dijkshoorn L,Ursing BM,Ursing JB

    更新日期:2000-05-01 00:00:00

  • A collaborative evaluation of a rapid, semi-automated identification system for gram-negative bacilli: the Quantum II BID.

    abstract::The recently-introduced semi-automated bacterial identification system, the Quantum II BID, is designed to identify fermentative and non-fermentative gram-negative bacilli in 4-5 h. The system was evaluated independently by the two participating laboratories. Inter-laboratory reproducibility was determined by testing ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-21-2-145

    authors: Stevens M,Henrichsen C,Smith M

    更新日期:1986-03-01 00:00:00

  • Cationic currents induced by Clostridium perfringens type A enterotoxin in human intestinal CaCO-2 cells.

    abstract::Clostridium perfringens type A produces an enterotoxin that induces diarrhoea experimentally in man and animals. The enterotoxin causes increased membrane permeability in susceptible cells which is thought to be due to pore formation in the host cell membrane. The effect of purified C. perfringens enterotoxin on intac...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-48-3-235

    authors: Hardy SP,Denmead M,Parekh N,Granum PE

    更新日期:1999-03-01 00:00:00

  • Rapid and accurate identification of species belonging to the Candida parapsilosis complex by real-time PCR and melting curve analysis.

    abstract::Candida parapsilosis is the second most frequent Candida species isolated from blood cultures. Since 2005, C. parapsilosis has been divided into three distinct species based on genetic traits: Candida parapsilosis, Candida metapsilosis and Candida orthopsilosis. The aim of this study was to develop a rapid real-time P...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.026633-0

    authors: Hays C,Duhamel C,Cattoir V,Bonhomme J

    更新日期:2011-04-01 00:00:00

  • There is no hiding if you Seq: recent breakthroughs in Pseudomonas aeruginosa research revealed by genomic and transcriptomic next-generation sequencing.

    abstract::The advent of next-generation sequencing technology has revolutionized the field of prokaryotic genetics and genomics by allowing interrogation of entire genomes, transcriptomes and global transcription factor binding profiles. As more studies employing these techniques have been performed, the state of the art regard...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章,评审

    doi:10.1099/jmm.0.001135

    authors: Valli RXE,Lyng M,Kirkpatrick CL

    更新日期:2020-02-01 00:00:00

  • A particle agglutination assay for rapid identification of heparin binding to coagulase-negative staphylococci.

    abstract::The heparin-binding properties of six different species of coagulase-negative staphylococci were examined by a particle agglutination assay. Heparin (mol. wt 4000-6000), mildly treated with sodium periodate, was covalently coupled to amino-modified latex beads (0.72 micron diameter). The particle agglutination assay w...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-45-4-263

    authors: Pascu C,Hirmo S,Ljungh A,Wadström T

    更新日期:1996-10-01 00:00:00

  • Enzymic activity of Haemophilus ducreyi.

    abstract::The enzymic activity of 29 Haemophilus ducreyi strains on 28 substrates is described. The results are compared with those of seven other authors. There is agreement only about the presence of alkaline phosphatase and arginine aminopeptidase and the lack of glycosidases. Possible reasons for the contradictions in the e...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-18-2-181

    authors: Sturm AW,Zanen HC

    更新日期:1984-10-01 00:00:00

  • Biochemical characterisation, enteropathogenicity and antimicrobial resistance plasmids of clinical and environmental Aeromonas isolates.

    abstract::One hundred and eight strains of Aeromonas from clinical and environmental samples were speciated. Seven species were identified, the most prevalent of which was A. hydrophila. Experimental studies in an animal model with 36 representative strains of different species revealed that all strains could cause significant ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-44-6-434

    authors: Chaudhury A,Nath G,Shukla BN,Sanyal SC

    更新日期:1996-06-01 00:00:00