Comparison of conventional culture with SeptiFast real-time PCR for microbial pathogen detection in clinical specimens other than blood.

abstract：:The abilities of six strains of Salmonella typhimurium to associate with rabbit ileal mucosa have been measured in vitro. Two were "virulent" strains (TML and W118 which are invasive and inducers of fluid secretion in rabbit ileal loops); four were "avirulent" (LT7, M206 and SL1027 which are invasive but induce neglig...

journal_title：Journal of medical microbiology

authors： Worton KJ,Candy DC,Wallis TS,Clarke GJ,Osborne MP,Haddon SJ,Stephen J

更新日期：1989-08-01 00:00:00

abstract：:Helicobacter pylori is classified as a class I carcinogenic factor and its persistent colonization in the stomach induces gastric cancer. Cancerous Inhibitor of PP2A (CIP2A) is a newly identified oncoprotein overexpressed in gastric cancer. Serving as a key oncoprotein, CIP2A also participates in regulation of senesce...

journal_title：Journal of medical microbiology

authors： Zhao D,Liu Z,Ding J,Li W,Sun Y,Yu H,Zhou Y,Zeng J,Chen C,Jia J

更新日期：2010-03-01 00:00:00

abstract：:Cryptococcosis causes disseminated disease in AIDS patients. In contrast to what occurs in laboratory conditions, a large capsule is produced by Cryptococcus neoformans in vivo during infection. The aim of this study was to compare the in vitro activity of different antifungal agents against 34 clinical isolates of C....

journal_title：Journal of medical microbiology

authors： Vitale RG,Pascuccelli V,Afeltra J

更新日期：2012-03-01 00:00:00

abstract：:Small round viruses (SRV) were isolated from the faeces of diarrhoeic calves from three farms. All three SRV preparations caused diarrhoea experimentally in gnotobiotic calves. Each preparation contained viral particles of two morphological types, "astrovirus-like" and "calicivirus-like", and from one preparation the ...

journal_title：Journal of medical microbiology

authors： Woode GN,Bridger JC

更新日期：1978-11-01 00:00:00

abstract：:Bovine lactoferricin, a pepsin-generated antimicrobial peptide from bovine lactoferrin active against a wide range of bacteria, was tested for its ability to influence the adhesion and invasion of Yersinia enterocolitica and Yersinia pseudotuberculosis in HEp-2 cells. The addition of non-cytotoxic and non-bactericidal...

journal_title：Journal of medical microbiology

authors： Di Biase AM,Tinari A,Pietrantoni A,Antonini G,Valenti P,Conte MP,Superti F

更新日期：2004-05-01 00:00:00

abstract：:The effect of glucan on Leishmania major infection was studied in BALB/c mice, which are highly susceptible to leishmania infection. Glucan (0.45 mg), or isovolumetric dextrose, was administered intraperitoneally 7, 5, 3 and 1 day before infection with L. major promastigotes. At 3, 5, 6, 8 and 10 weeks after infection...

journal_title：Journal of medical microbiology

authors： Al Tuwaijri AS,Mahmoud AA,Al Mofleh IA,Al Khuwaitir SA

更新日期：1987-06-01 00:00:00

abstract：:The surface structures of the cell envelopes of 16 clinical isolates of Pseudomonas aeruginosa were examined by electronmicroscopy with the new fixation technique of freeze-substitution. Two types of structures were observed among the organisms. In one group of strains, mostly isolated from blood, a dense fibrous laye...

journal_title：Journal of medical microbiology

authors： Misumi H,Umeda A,Eguchi K,Okada K,Sawae Y,Niho Y,Amako K

更新日期：1994-04-01 00:00:00

abstract：:Herpes simplex virus type-1 (HSV-1) induces Fc- and C3b(i)-receptors on infected cells. The role of these receptors in bacterial superinfection was studied by comparing the adherence of non-opsonised and opsonised bacteria to HSV-infected and non-infected HEp-2 cells. A flow cytometric adherence assay, based on the fl...

journal_title：Journal of medical microbiology

authors： De Graaf-Miltenburg LA,Van Vliet KE,Ten Hagen TL,Verhoef J,Van Strijp JA

更新日期：1994-01-01 00:00:00

abstract：:Detection of latent Mycobacterium tuberculosis is a challenge in the diagnosis of asymptomatic, subclinical tuberculosis. We report the development of an immunofluorescence technique to visualize and enumerate M. tuberculosis in latently infected rabbit lungs where no acid-fast-stained organisms were seen and no culti...

journal_title：Journal of medical microbiology

authors： Subbian S,Eugenin E,Kaplan G

更新日期：2014-11-01 00:00:00

abstract：:Helicobacter pylori is believed to trigger neutrophil activation through several factors, including the H. pylori neutrophil-activating protein (HpNAP). The aim of this study was to characterise the factors within H. pylori cell-free extracts that stimulate neutrophil activation. Neutrophil activation was found to be ...

journal_title：Journal of medical microbiology

authors： Leakey A,Hirst R,LA Brooy J

更新日期：2001-09-01 00:00:00

abstract：:The prevalence of community-acquired meticillin-resistant Staphylococcus aureus (CA-MRSA) strains has become a serious problem worldwide. The aim of this study was to investigate the annual transitions of MRSA strains with the CA-MRSA feature, which were identified as SCCmec type IV or V, in a hospital setting in Japa...

journal_title：Journal of medical microbiology

authors： Ito A,Nakaminami H,Fujii T,Utsumi K,Noguchi N

更新日期：2015-07-01 00:00:00

abstract：:The aim of this study was to improve the identification of Mycobacterium species in the context of a UK teaching hospital. Real-time PCR assays were established to enable the rapid differentiation between Mycobacterium tuberculosis (MTB) complex and Mycobacterium species other than tuberculosis (MOTT), followed by 16S...

journal_title：Journal of medical microbiology

authors： Williams KJ,Ling CL,Jenkins C,Gillespie SH,McHugh TD

更新日期：2007-05-01 00:00:00

abstract：PURPOSE:This study aimed to detect and identify staphylococcal enterotoxin (SE) genes in methicillin-resistant Staphylococcus pseudintermedius (MRSP) strains from different sources, and to investigate the relationship between their sequence types (STs) and SE gene patterns. METHODOLOGY:The profiles of 17 SE genes in 9...

journal_title：Journal of medical microbiology

authors： Phumthanakorn N,Fungwithaya P,Chanchaithong P,Prapasarakul N

更新日期：2018-06-01 00:00:00

abstract：:Of 200 isolates of Vibrio mimicus screened, one from water (N-57) agglutinated with V. cholerae O139 polyclonal antiserum (absorbed with a rough strain of V. cholerae only) and not with O139 polyclonal diagnostic antiserum (absorbed with the rough strain and V. cholerae O22 and O155). The antigenic relationship betwee...

journal_title：Journal of medical microbiology

authors： Ansaruzzaman M,Shimada T,Bhuiyan NA,Nahar S,Alam K,Islam MS,Albert MJ

更新日期：1999-09-01 00:00:00

abstract：:CaNdt80p, the Candida albicans homologue of the Saccharomyces cerevisiae transcription factor ScNdt80p, has been identified as a positive regulator of CDR1, which encodes an efflux pump involved in drug resistance in C. albicans. To investigate the involvement of the putative DNA-binding domain of CaNdt80p in drug res...

journal_title：Journal of medical microbiology

authors： Wang JS,Yang YL,Wu CJ,Ouyang KJ,Tseng KY,Chen CG,Wang H,Lo HJ

更新日期：2006-10-01 00:00:00

abstract：:Ten recent clinical isolates of Salmonella serotype Typhimurium from man that were tested for their invasiveness in rabbit ileal explants in vitro, were compared with Typhimurium strain TML, a well-characterised invasive strain isolated from a case of human gastro-enteritis. Nine of the 10 strains showed invasiveness ...

journal_title：Journal of medical microbiology

authors： Bolton AJ,Martin GD,Osborne MP,Wallis TS,Stephen J

更新日期：1999-09-01 00:00:00

abstract：:A detailed study was made of lambs aged 5--7 months naturally infected with Pasteurella haemolytica biotype T. In addition to the well known features of such infections, previously unreported necrotic lesions of the tonsil, oesophagus, pharynx and adjacent areas were consistently seen. Large numbers of P. haemolytica ...

journal_title：Journal of medical microbiology

authors： Dyson DA,Gilmour NJ,Angus KW

更新日期：1981-02-01 00:00:00

abstract：:An outer-membrane protein (OMP) was isolated from a clinical strain of Bacteroides distasonis. Changes in growth media did not appreciably affect the appearance of this protein in crude outer-membrane preparations examined by SDS-PAGE. However, the proportion of the protein relative to other OMPs was greater in 24-h c...

journal_title：Journal of medical microbiology

authors： Wexler HM,Getty C,Fisher G

更新日期：1992-09-01 00:00:00

abstract：:Polyacrylamide-agarose gel electrophoresis was used to study polymorphism of lactate dehydrogenase (LDH), nucleoside phosphorylase (NSP), phosphoglucose isomerase (PGI), hydroxybutyrate dehydrogenase (HBD), adenylate kinase (ADK) and esterases of 44 strains of Streptococcus pyogenes, 25 group G streptococcal strains, ...

journal_title：Journal of medical microbiology

authors： Bert F,Picard B,Lambert-Zechovsky N,Goullet P

更新日期：1995-06-01 00:00:00

abstract：:The unpredictable characteristics of meningococcal disease (MD) make outbreaks complicated to monitor and consequently lead to high levels of public anxiety. Traditional molecular techniques have been utilized in order to understand better the epidemiology of MD, but some have disadvantages such as being highly specia...

journal_title：Journal of medical microbiology

authors： Diggle MA,Bell CM,Clarke SC

更新日期：2003-06-01 00:00:00

abstract：:Helicobacter pylori and Proteus mirabilis ureases are nickel-requiring metallo-enzymes that hydrolyse urea to NH3 and CO2. In both H. pylori and in an Escherichia coli model of H. pylori urease activity, a high affinity nickel transporter, NixA, is required for optimal urease activity, whereas the urea-dependent UreR ...

journal_title：Journal of medical microbiology

authors： McGEE DJ,Coker C,Testerman TL,Harro JM,Gibson SV,Mobley HLT

更新日期：2002-11-01 00:00:00

abstract：:The plasmid profiles, and their association with antimicrobial resistance, of 60 strains of Aeromonas hydrophila isolated from fish, shellfish and water were investigated. Only two strains were susceptible to all the antimicrobial agents tested; the highest incidences of resistance were to tetracycline (96.7%), prysta...

journal_title：Journal of medical microbiology

authors： Borrego JJ,Moriñigo MA,Martinez-Manzanares E,Bosca M,Castro D,Barja JL,Toranzo AE

更新日期：1991-11-01 00:00:00

abstract：:In January 2015, Public Health England and the United Kingdom (UK) Ministry of Defence investigated cases of diarrhoea and fever in military personnel recently returned to the UK after supporting the response to the Ebola epidemic in Sierra Leone. Tests for Ebola virus infection were negative. PCR tests detected the i...

journal_title：Journal of medical microbiology

authors： Todkill D,Pudney R,Terrell A,Tuck J,Hutley E,Dallman TJ,Jenkins C,Manuel R,Wade M,Puleston R,Hawker J

更新日期：2018-11-01 00:00:00

abstract：:Chronic meningococcaemia is an unusual clinical presentation of Neisseria meningitidis infection. We describe the case of a patient, who presented with total IgA deficiency and partial IgM deficiency with a low switched memory B cells count, suggestive of a borderline form of common variable immunodeficiency (CVID). T...

journal_title：Journal of medical microbiology

authors： Theulin A,Rondeau-Lutz M,Kuhnert C,Boileau J,Weber JC

更新日期：2010-11-01 00:00:00

abstract：:Diagnosis of human immunodeficiency virus (HIV) infection by antibody-based testing allows for some recently infected individuals to be falsely assessed as non-infected. Since such individuals often have high viral loads and are capable of transmitting HIV, it is an imperative public health need to identify these indi...

journal_title：Journal of medical microbiology

authors： Ren A,Louie B,Rauch L,Castro L,Liska S,Klausner JD,Pandori MW

更新日期：2008-10-01 00:00:00

abstract：:Rapid diagnosis of multidrug-resistant tuberculosis (MDR-TB) is essential for the prompt initiation of effective second-line therapy to improve treatment outcome and limit transmission of this obstinate disease. A variety of molecular methods that enable the rapid detection of mutations implicated in MDR-TB have been ...

journal_title：Journal of medical microbiology

authors： Bang H,Park S,Hwang J,Jin H,Cho E,Kim DY,Song T,Shamputa IC,Via LE,Barry CE,Cho SN,Lee H

更新日期：2011-10-01 00:00:00

abstract：:This study attempted to determine whether cefuroxime was superior to cephalothin as a surrogate marker for cefpodoxime among urinary tract isolates. The MicroScan system (Siemens) was used to determine susceptibility for cephalothin and cefuroxime on consecutive cultures with a colony count of ≥50 000 organisms. Simul...

journal_title：Journal of medical microbiology

authors： Bookstaver DA,Bland CM,Woodberry MW,Mansell KB

更新日期：2014-02-01 00:00:00

abstract：:Recent studies have shown that Providencia alcalifaciens is a diarrhoeal pathogen. It may cause diarrhoea by an invasive mechanism, as it invades cultured mammalian cells in vitro and intestinal epithelial cells of experimentally inoculated rabbits in vivo. To locate the gene(s) involved in invasion, TnphoA mutants of...

journal_title：Journal of medical microbiology

authors： Rahman M,Monira S,Nahar S,Ansaruzzaman M,Alam K,Alam M,Albert MJ

更新日期：2002-08-01 00:00:00

abstract：:Streptococcus oralis has emerged as one of the most important organisms of the viridans streptococcus group in terms of infections and is recognised as an agent of infective endocarditis and, in immunocompromised patients, septicaemia. The mechanisms by which this organism proliferates in vivo are unknown. However, ho...

journal_title：Journal of medical microbiology

authors： Byers HL,Homer KA,Tarelli E,Beighton D

更新日期：1999-04-01 00:00:00

abstract：:This prospective study investigated whether oral candidal carriage in HIV-infected patients was altered by pentamidine therapy. Repeated oral rinses were taken from 56 HIV-positive patients over a 2-year period. Oral candidal carriage was investigated in two groups of patients, one receiving prophylactic pentamidine t...

journal_title：Journal of medical microbiology

authors： Nolan A,Lamey PJ,MacFarlane TW,Aitchison TC,Shaw J,Sirel JY

更新日期：1994-08-01 00:00:00