Nucleotide sequence-based typing of meningococci directly from clinical samples.

abstract：:The aim of this study was to establish bacterial profiles in gastric biopsy specimens from patients with Helicobacter pylori-associated gastritis by means of temporal temperature gradient gel electrophoresis (TTGE) of PCR-amplified 16S rDNA fragments. Specimens from eight patients with asymptomatic gastritis and five ...

journal_title：Journal of medical microbiology

authors： Monstein HJ,Tiveljung A,Kraft CH,Borch K,Jonasson J

更新日期：2000-09-01 00:00:00

abstract：PURPOSE:We aimed to evaluate a PCR-based technique for the diagnosis of mucormycosis and the identification of fungi from fresh tissue specimens in patients with rhino-orbito-cerebral-mucormycosis (ROCM). METHODOLOGY:Fifty cases of ROCM were included in the study. Conventional identification was performed using micros...

journal_title：Journal of medical microbiology

authors： Zaman K,Rudramurthy SM,Das A,Panda N,Honnavar P,Kaur H,Chakrabarti A

更新日期：2017-08-01 00:00:00

abstract：:The cell-wall characteristics of Gardnerella vaginalis grown in conventional and biofilm systems were studied by electron microscopy. The gram-positive nature of the cell wall was confirmed. Novel cell-wall particles which appeared to be associated with cell division were also identified, particularly in organisms of ...

journal_title：Journal of medical microbiology

authors： Muli FW,Struthers JK,Tarpey PA

更新日期：1999-02-01 00:00:00

abstract：:Saksenaea erythrospora is a species of the order Mucorales recently described and reported as a cause of human mucormycosis. We report a case of S. erythrospora in a man involved in a serious sailing accident causing deep skin and soft tissue contamination with soil and water. Direct microscopic examination of the cli...

journal_title：Journal of medical microbiology

authors： Relloso S,Romano V,Landaburu MF,Herrera F,Smayevsky J,Veciño C,Mujica MT

更新日期：2014-02-01 00:00:00

abstract：:A total of 817 human clinical isolates of Clostridium difficile from all Australian states were screened for A(-)B(+) strains by toxin gene PCR assays. Nine (1.1 %) strains were confirmed to be A(-)B(+) by enzyme immunoassay for toxin production. Of these, six (66.7 %) were binary toxin-positive by PCR. Using PCR ribo...

journal_title：Journal of medical microbiology

authors： Elliott B,Squire MM,Thean S,Chang BJ,Brazier JS,Rupnik M,Riley TV

更新日期：2011-08-01 00:00:00

abstract：:A total of 287 Acinetobacter isolates belonging to DNA groups 2 (A. baumannii) and 13TU was collected consecutively from 46 hospitals and typed by randomly amplified polymorphic DNA fingerprinting with primers DAF-4 and ERIC-2. With a similarity coefficient of >/=72% as a cut-off value, 37 clusters of genotypically si...

journal_title：Journal of medical microbiology

authors： Spence RP,Towner KJ,Henwood CJ,James D,Woodford N,Livermore DM

更新日期：2002-12-01 00:00:00

abstract：:Limited digestion of staphylococcal enterotoxin B (SEB) with trypsin resulted in the generation of a 12-Kda amino-terminal fragment and a 17-Kda carboxy-terminal fragment which were isolated by preparative iso-electric focusing. The carboxy-terminal fragment exhibited significant mitogenic activity for murine splenocy...

journal_title：Journal of medical microbiology

authors： Binek M,Newcomb JR,Rogers CM,Rogers TJ

更新日期：1992-03-01 00:00:00

abstract：:Several imipenem-susceptible and -resistant Aeromonas veronii biovar sobria isolates with different morphologies and antimicrobial susceptibilities recovered from bile samples of a patient with cholangitis were analysed. These isolates belonged to the same clone and the imipenem-resistant strains showed overexpression...

journal_title：Journal of medical microbiology

authors： Sánchez-Céspedes J,Figueras MJ,Aspiroz C,Aldea MJ,Toledo M,Alperí A,Marco F,Vila J

更新日期：2009-04-01 00:00:00

abstract：:Many micro-organisms cause important diseases of the female genital tract. Because systematic vaccination does not usually provide a good immune response at mucosal sites, commensal lactobacilli from the female genital tract were developed as vehicles to deliver continued doses of foreign antigen directly to the genit...

journal_title：Journal of medical microbiology

authors： Rush CM,Hafner LM,Timms P

更新日期：1994-10-01 00:00:00

abstract：:An evaluation of five ELISA methods for the diagnosis of acute toxoplasmosis was undertaken by comparing a laboratory-produced ELISA employing two recombinant Toxoplasma gondii polypeptides as antigen with a laboratory-produced ELISA and three commercially available ELISAs employing traditional parasite antigen prepar...

journal_title：Journal of medical microbiology

authors： Johnson AM,Roberts H,Tenter AM

更新日期：1992-12-01 00:00:00

abstract：:A multiplex PCR was developed to detect 15 serotypes of Streptococcus suis. This multiplex PCR was separated into two reaction sets. The first set identified nine serotypes (serotypes 1/2, 1, 2, 3, 4, 7, 9, 14 and 16) and the second set identified six serotypes (serotypes 5, 8, 10, 19, 23 and 25). This assay correctly...

journal_title：Journal of medical microbiology

authors： Kerdsin A,Dejsirilert S,Akeda Y,Sekizaki T,Hamada S,Gottschalk M,Oishi K

更新日期：2012-12-01 00:00:00

abstract：:A slide agglutination technique identifying 10 serogroups of Clostridium difficile (A,B,C,D,F,G,H,I,K and X) has been described previously. In this study, we have used the hamster to compare the ability of the 10 serogroup reference strains to colonise and produce disease. Groups of four hamsters were each given a sin...

journal_title：Journal of medical microbiology

authors： Delmée M,Avesani V

更新日期：1990-10-01 00:00:00

abstract：:Campylobacter jejuni is a frequent cause of infectious diarrhoea and is increasingly recognized as a trigger for late-onset complications. The poor standardization of commonly used serological tests might explain the conflicting results regarding the frequency of antecedent C. jejuni infections in defined patient grou...

journal_title：Journal of medical microbiology

authors： Schmidt-Ott R,Brass F,Scholz C,Werner C,Groß U

更新日期：2005-08-01 00:00:00

abstract：:During 2003, Vibrio cholerae O1 Ogawa was the predominant serotype among diarrhoeal patients admitted to different hospitals in India. With the exception of 3 strains from Kolkata, none of 172 strains examined exhibited resistance to tetracycline, but 45.7 % showed reduced susceptibility to ciprofloxacin. Extensive mo...

journal_title：Journal of medical microbiology

authors： Chatterjee S,Ghosh K,Raychoudhuri A,Pan A,Bhattacharya MK,Mukhopadhyay AK,Ramamurthy T,Bhattacharya SK,Nandy RK

更新日期：2007-06-01 00:00:00

abstract：:Cross-reactivity in a delayed-type hypersensitivity (DH) response was studied in mice immunised with live Salmonella typhi, S. paratyphi A and S. paratyphi B. Extensive cross-reactions outside the serogroup limits were observed. The ability of DH cross-reacting and non-cross-reacting sonicates to generate activated ma...

journal_title：Journal of medical microbiology

authors： Tiwari H,Kamat RS

更新日期：1986-05-01 00:00:00

abstract：:The aim of this study was to examine changes in the systemic immune response during the incubation period and following the onset of clinical swine dysentery, including the recovery period. Ten healthy conventional pigs were inoculated with Brachyspira hyodysenteriae. Blood was sampled at pre-inoculation, at days 4 an...

journal_title：Journal of medical microbiology

authors： Jonasson R,Andersson M,Råsbäck T,Johannisson A,Jensen-Waern M

更新日期：2006-07-01 00:00:00

abstract：:Radiometric selective inhibition tests were developed and evaluated for the rapid differentiation of Mycobacterium spp. Both a p-nitrobenzoic acid (PNB) test and a commercially-prepared p-nitro-alpha-acetylamino-beta-hydroxypropiophenone (NAP) test successfully differentiated M. tuberculosis and M. bovis from "atypica...

journal_title：Journal of medical microbiology

authors： Collins T,Levett PN

更新日期：1989-11-01 00:00:00

abstract：:Nosocomial infections with enterococci are an increasing problem in modern medical practice due to the development of resistance to a wide range of antibiotics, including the glycopeptides vancomycin and teicoplanin. An increasing number of vancomycin-resistant enterococci (VRE) have been cultured from clinical specim...

journal_title：Journal of medical microbiology

authors： Mackenzie CR,Hucke C,MÜller D,Seidel K,Takikawa O,DÄubener W

更新日期：1999-10-01 00:00:00

abstract：:We have characterised 45 isolates of methicillin-resistant Staphylococcus aureus (MRSA) from Glasgow Royal Infirmary by means of simple biotyping, immunoblotting of exported proteins and restriction enzyme fragmentation patterns (REFP) of plasmid DNA. The strains were subdivided into four groups (A-D) on the basis of ...

journal_title：Journal of medical microbiology

authors： Coia JE,Thomson-Carter F,Baird D,Platt DJ

更新日期：1990-02-01 00:00:00

abstract：:This study investigated the influence of the carbon source of the growth medium, strains of Candida albicans and source of epithelial cells, and the influence of smoking and gender, on the adhesion of C. albicans to epithelial cells from insulin-using diabetic patients. Adhesion was determined by an autologous adhesio...

journal_title：Journal of medical microbiology

authors： Willis AM,Coulter WA,Hayes JR,Bell P,Lamey PJ

更新日期：2000-03-01 00:00:00

abstract：:The in vitro activity of voriconazole was compared with those of fluconazole and itraconazole against 270 clinical isolates of yeasts from the mouths of patients receiving palliative care for advanced cancer. A broth micro-dilution assay as described by the National Committee for Clinical Laboratory Standards was empl...

journal_title：Journal of medical microbiology

authors： Bagg J,Sweeney MP,Davies AN,Jackson MS,Brailsford S

更新日期：2005-10-01 00:00:00

abstract：:The susceptibility to teicoplanin, vancomycin and linezolid of 30 clinical isolates of Enterococcus faecium was tested by Vitek 2, Phoenix, Etest, broth microdilution and disc diffusion tests. The vanA and vanB resistance genes and the 23S rRNA gene G2576T mutation were detected by PCR and PCR-RFLP, respectively. Resi...

journal_title：Journal of medical microbiology

authors： Raponi G,Ghezzi MC,Gherardi G,Lorino G,Dicuonzo G

更新日期：2010-06-01 00:00:00

abstract：:In order to develop novel host/pathogen real-time PCR assays for routine diagnostic use, early gene expression patterns from both Epstein-Barr virus (EBV) and Raji cells were examined after inducing the lytic life cycle using 12-O-tetradecanoyl-13-phorbol ester and sodium butyrate. Real-time PCR identified several hig...

journal_title：Journal of medical microbiology

authors： Auburn H,Zuckerman M,Smith M

更新日期：2016-11-01 00:00:00

abstract：:The effect of glucan on Leishmania major infection was studied in BALB/c mice, which are highly susceptible to leishmania infection. Glucan (0.45 mg), or isovolumetric dextrose, was administered intraperitoneally 7, 5, 3 and 1 day before infection with L. major promastigotes. At 3, 5, 6, 8 and 10 weeks after infection...

journal_title：Journal of medical microbiology

authors： Al Tuwaijri AS,Mahmoud AA,Al Mofleh IA,Al Khuwaitir SA

更新日期：1987-06-01 00:00:00

abstract：:A rabbit was immunised with a heat-killed vaccine prepared from strain 1142 of Staphylococcus epidermidis. The HA titres of the antisera against a surface-polysaccharide antigen extracted from the homologous strain were highest in the 2nd-4th weeks after immunisation and subsequently declined. Only a minor amount of 2...

journal_title：Journal of medical microbiology

authors： Yoshida K,Ichiman Y

更新日期：1978-11-01 00:00:00

abstract：:In this study, we evaluated the Sofia Streptococcus pneumoniae FIA test (Quidel Corporation, San Diego, CA, USA), a new immunofluorescence-based lateral flow test for the qualitative detection of S. pneumoniae antigen in urine or cerebrospinal fluid specimens. The analyses of 100 non-concentrated urine samples (includ...

journal_title：Journal of medical microbiology

authors： Euser SM,Badoux P,Kracht-Kosten L,Yzerman EPF

更新日期：2018-12-01 00:00:00

abstract：:Clostridium difficile-associated diarrhoea (CDAD) is the most common hospital-acquired diarrhoea, and is a major type of gastroenteritis infection in nursing homes and facilities for the elderly. In this study the antimicrobial activity of the two-component lantibiotic, lacticin 3,147, against a range of genetically d...

journal_title：Journal of medical microbiology

authors： Rea MC,Clayton E,O'Connor PM,Shanahan F,Kiely B,Ross RP,Hill C

更新日期：2007-07-01 00:00:00

abstract：:Intravenous injection of eight human strains of Campylobacter fetus ss fetus and Campylobacter jejuni into mice at various stages of pregnancy demonstrated significant strain differences in ability to affect implantation of the fertilised ovum and to cause resorption of the mouse fetus. Implantation was significantly ...

journal_title：Journal of medical microbiology

authors： O'Sullivan AM,Doré CJ,Coid CR

更新日期：1988-01-01 00:00:00

abstract：:Staphylococcus aureus produces numerous bi-component toxins, e.g., Panton-Valentine leukocidin (Luk-PVL) and gamma-haemolysin, which consist of type S and F proteins. Previous studies showed that Luk-PVL induces inflammatory mediator release from human granulocytes that might reflect the in-vivo effects, e.g., dermone...

journal_title：Journal of medical microbiology

authors： König B,Prévost G,König W

更新日期：1997-06-01 00:00:00

abstract：:Conventional culture and drug susceptibility testing (DST) methods for Mycobacterium tuberculosis are laborious and time consuming. For this reason alternative rapid culture and DST techniques are urgently needed to shorten the time for drug-resistance detection. A total of 222 smear-positive sputum samples were evalu...

journal_title：Journal of medical microbiology

authors： Imperiale BR,Morcillo NS,Palomino JC,Vandamme P,Martin A

更新日期：2014-04-01 00:00:00