Abstract:
:Lancefield acid extracts of Streptococcus pyogenes, type 22 (T12, M22, OF positive) gave good yields of M protein and little opacity factor (OF), but sodium dodecyl sulphate (SDS) extracts contained high titres of OF (greater than 20000) and little M protein. Acid-extracted OF could be separated from M protein by Sepharose 4B chromatography, but some of the OF-positive fractions that did not precipitate with the absorbed homologous anti-M rabbit serum, were able to neutralise opsonic antibodies present in human serum. The isoelectric-focusing profiles of the two antigens showed partial similarity. Some strains of the OF-positive serotypes, e.g., M-types 22 and 49, lost both M antigen and OF activity on serial transfer in Todd-Hewitt broth, but this was not seen in a representative of M-type 60, and no M-negative OF-negative variants could be detected after six subcultures. Among the OF-negative serotypes some, e.g., M-types 5 and 6, were completely stable, whereas others, e.g., M-types 12, 55 and 57, lost their M antigens after serial subculture. One explanation is that the genes that code for M antigen are plasmid borne in some serotypes and, moreover, are carried on the same plasmid as the gene for OF in some OF-positive serotypes. However, analysis of cell lysates by agarose-gel electrophoresis failed to demonstrate the presence of plasmid DNA in any of the strains tested.
journal_name
J Med Microbioljournal_title
Journal of medical microbiologyauthors
Hallas G,Widdowson JPdoi
10.1099/00222615-16-1-13subject
Has Abstractpub_date
1983-02-01 00:00:00pages
13-26issue
1eissn
0022-2615issn
1473-5644journal_volume
16pub_type
杂志文章abstract::In tests of bactericidal action against H. influenzae type b strains isolated from patients with meningitis, chloramphenicol was found to be far more reliable than ampicillin in dealing with large inocula, and more rapidly effective against both large and relatively small inocula. These findings provide a laboratory e...
journal_title:Journal of medical microbiology
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doi:10.1099/00222615-10-1-127
更新日期:1977-02-01 00:00:00
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更新日期:2001-07-01 00:00:00
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journal_title:Journal of medical microbiology
pub_type: 杂志文章
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abstract::A co-agglutination (Co-A) test with Staphylococcus aureus (Cowan's strain I) bearing protein A coated with specific hydatid antibodies, was used to demonstrate circulating hydatid antigen in serum for the diagnosis of hydatid disease. The test had a sensitivity of 95% and specificity of 89% in detecting hydatid antige...
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journal_title:Journal of medical microbiology
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doi:10.1099/00222615-42-4-237
更新日期:1995-04-01 00:00:00
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更新日期:2007-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2002-09-01 00:00:00
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journal_title:Journal of medical microbiology
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更新日期:2007-12-01 00:00:00
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更新日期:2013-06-01 00:00:00
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journal_title:Journal of medical microbiology
pub_type: 杂志文章,评审
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更新日期:2000-05-01 00:00:00
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journal_title:Journal of medical microbiology
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doi:10.1099/00222615-47-2-99
更新日期:1998-02-01 00:00:00
abstract::The objectives of the present study were to evaluate in vitro the production of antagonistic compounds against Gardnerella vaginalis by Lactobacillus strains isolated from women with or without bacterial vaginosis (BV), and to select one of the better Lactobacillus producers of such a substance to be tested in vivo us...
journal_title:Journal of medical microbiology
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更新日期:2012-08-01 00:00:00
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journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-47-9-781
更新日期:1998-09-01 00:00:00
abstract::The growth inhibiting activity of transferrins, citrate, 2-2' dipyridyl and desferrioxamine methanesulphonate towards Legionella spp. and their serogroups was investigated. The inhibitory activity of all these compounds depended upon the iron-free state of the molecules and was abolished by saturation with iron. No ba...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-34-2-113
更新日期:1991-02-01 00:00:00
abstract::Early reports of Candida krusei in man describe the organism as a transient, infrequent isolate of minor clinical significance inhabiting the mucosal surfaces. More recently it has emerged as a notable pathogen with a spectrum of clinical manifestations such as fungaemia, endophthalmitis, arthritis and endocarditis, m...
journal_title:Journal of medical microbiology
pub_type: 杂志文章,评审
doi:10.1099/00222615-41-5-295
更新日期:1994-11-01 00:00:00
abstract::Intratracheal administration of purified Pseudomonas aeruginosa exoenzyme S elicited extensive, grossly observable damage in the rat lung within 2 h. Light and electronmicroscopy revealed injury and necrosis of bronchial epithelium, type I pneumocytes and capillary endothelial cells after 1 h; associated haemorrhage, ...
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更新日期:1988-06-01 00:00:00
abstract::Cutaneous leishmaniasis (CL), a neglected tropical disease, is reported to be prevalent in tribal villages located in the Agasthyamala Biosphere Reserve forests of Western Ghats, Kerala state, India. We carried out an investigation to characterize the species of Leishmania parasites involved in these infections preval...
journal_title:Journal of medical microbiology
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更新日期:2015-02-01 00:00:00
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journal_title:Journal of medical microbiology
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更新日期:1977-08-01 00:00:00
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journal_title:Journal of medical microbiology
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更新日期:2003-03-01 00:00:00
abstract:PURPOSE:Meticillin-resistant Staphylococcus aureus (MRSA) biofilm formation in humans is of serious clinical concern. Previous in vitro studies have been performed with biofilms grown only on inorganic substrates; therefore, we investigated the vancomycin (VCM) resistance of MRSA biofilms grown on skin tissue. METHODO...
journal_title:Journal of medical microbiology
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journal_title:Journal of medical microbiology
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更新日期:2009-01-01 00:00:00
abstract::After the introduction of the dengue-2 (16681) virus by intradermal (i.d.) injection into the footpads of mice, Langerhans cells (LCs) increased in numbers within 24 h at the site of injection and neutralising antibody developed. On comparing the i.d. and intramuscular (i.m.) routes, antibody was produced more rapidly...
journal_title:Journal of medical microbiology
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更新日期:1996-08-01 00:00:00
abstract::Although Neisseria gonorrhoeae can use haem as the sole exogenous iron source for growth in vitro, the mechanism of haem-iron uptake in the gonococcus is unknown. Two haemin-binding proteins (HmBPs) of 97 and 44 Kda were isolated by batch ligand affinity-chromatography from whole cells or total membranes of gonococci ...
journal_title:Journal of medical microbiology
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更新日期:1992-02-01 00:00:00
abstract::Microcirculation plays a crucial role in mucosal physiological function as well as repair of gastric mucosal damage. Endothelial cell damage is known to disturb microcirculation and suppress angiogenesis. Therefore, the direct effect of Helicobacter pylori on endothelial cells in vitro was investigated with H. pylori ...
journal_title:Journal of medical microbiology
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更新日期:2002-05-01 00:00:00
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journal_title:Journal of medical microbiology
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更新日期:2013-06-01 00:00:00
abstract::The acid end-products of 185 isolates from the family Bacteroidaceae were separated and analysed by gas-liquid chromatography on broth cultures. Different media were evaluated and definitive studies were performed in a fully supplemented complex medium. The limitations of this approach to the identification of a wide ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
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更新日期:1978-05-01 00:00:00
abstract::Campylobacter fetus causes severe infections in humans and can be isolated from various mammals and reptiles. However, although poultry are considered to be the main reservoir of Campylobacter jejuni, little is known about the presence of C. fetus in poultry. Thus, specific pathogen-free chickens were experimentally i...
journal_title:Journal of medical microbiology
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