A Helicobacter hepaticus catalase mutant is hypersensitive to oxidative stress and suffers increased DNA damage.

Abstract:

:Catalase (KatA) is known to play an important role in oxidative stress resistance in many bacterial species and a homologue exists in Helicobacter hepaticus, a member of the enterohepatic Helicobacter species. Here, a katA mutant was constructed by insertional mutagenesis and its oxidative stress phenotype was investigated. Catalase activity was readily detected [196 units (mg protein crude cell extract)(-1)] in the wild-type, whereas the mutant strain was deficient in, but not devoid of, activity. In contrast, Helicobacter pylori katA strains lack detectable catalase activity and wild-type H. pylori generally contains higher specific activity than H. hepaticus. Wild-type H. hepaticus cells tolerated 6 % O2 for growth, whilst the katA mutant could not survive at this oxygen level. Even at the optimal O2 level, the growth of the H. hepaticus katA strain was severely inhibited, which is also in contrast to H. pylori katA strains. Wild-type H. hepaticus cells withstood exposure to 100 mM H(2)O(2) but the katA mutant cells were killed by the same treatment. Wild-type cells suffered no significant DNA damage by H(2)O(2) treatment (100 mM for 6 min), whilst the same treatment resulted in severe DNA fragmentation in the katA mutant. Thus H. hepaticus KatA plays an important role as an antioxidant protein.

journal_name

J Med Microbiol

authors

Hong Y,Wang G,Maier RJ

doi

10.1099/jmm.0.46891-0

subject

Has Abstract

pub_date

2007-04-01 00:00:00

pages

557-562

issue

Pt 4

eissn

0022-2615

issn

1473-5644

journal_volume

56

pub_type

杂志文章
  • Identification by PCR of Helicobacter pylori in subgingival plaque of adult periodontitis patients.

    abstract::The PCR was used to detect the presence of Helicobacter pylori in subgingival plaque samples from patients with adult periodontitis. Primers based upon the 16S ribosomal RNA (rRNA) gene sequence of H. pylori were used in a single round of PCR to amplify a 295-bp DNA fragment and the identity of the amplified products ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-48-3-317

    authors: Riggio MP,Lennon A

    更新日期:1999-03-01 00:00:00

  • Two different types of humoral immune response to Actinobacillus actinomycetemcomitans in high-responder periodontitis patients.

    abstract::Actinobacillus actinomycetemcomitans is considered to be an aetiological agent in various forms of periodontitis, with serotype b-specific carbohydrate being the immunodominant antigen of A. actinomycetemcomitans Y4 in high-responder patients. Lipopolysaccharide (LPS) of the organism may also be an important antigen. ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-47-7-569

    authors: Nakashima K,Usui C,Koseki T,Nishihara T,Ishikawa I

    更新日期:1998-07-01 00:00:00

  • Development of a routine laboratory direct detection system of staphylococcal enterotoxin genes.

    abstract::A novel direct detection system has been developed for eight staphylococcal enterotoxin (SE)-encoding genes (sea, seb, sec, sed, see, seg, seh and sei) in milk. Specific detection by real-time PCR was successful for all SE-encoding genes in the reference strains. Furthermore, a novel DNA-preparation method with good r...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.46027-0

    authors: Nakayama A,Okayama A,Hashida M,Yamamoto Y,Takebe H,Ohnaka T,Tanaka T,Imai S

    更新日期:2006-03-01 00:00:00

  • Mycobacterium conceptionense infection complicating face rejuvenation with fat grafting.

    abstract::We report a third case of Mycobacterium conceptionense infection, which was found in a 50-year-old female following face rejuvenation surgery with fat grafting. The pathogen was identified using 16S rRNA gene and rpoB gene sequences. The growing diversity of non-tuberculosis mycobacterial species causing human infecti...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.024554-0

    authors: Yang HJ,Yim HW,Lee MY,Ko KS,Yoon HJ

    更新日期:2011-03-01 00:00:00

  • Genotyping of the prevalent Chlamydia trachomatis strains involved in cervical infections in women in Ahvaz, Iran.

    abstract::To determine the prevalence of cervical Chlamydia trachomatis genotypes in Iran for the first time and their association with three clinical symptoms/signs, i.e. abnormal vaginal discharge, lower abdominal pain (LAP) and swab-induced bleeding, and patient age, 620 cervical specimens were obtained from women with sympt...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.016717-0

    authors: Beni BT,Motamedi H,Ardakani MR

    更新日期:2010-09-01 00:00:00

  • The bactericidal activity of DR-3355, an optically active isomer of ofloxacin.

    abstract::The bactericidal activity of compound DR-3355, an optically active isomer of ofloxacin, was measured against Escherichia coli, Staphylococcus aureus and S. epidermidis, in nutrient broth and in phosphate-buffered saline. DR-3355 was found to be approximately twice as active as ofloxacin in terms of the concentration a...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-30-3-227

    authors: Lewin CS,Amyes SG

    更新日期:1989-11-01 00:00:00

  • Hyperinvasiveness and increased intercellular spread of Listeria monocytogenes sequence type 1 are independent of listeriolysin S, internalin F and internalin J1.

    abstract:PURPOSE:Listeria monocytogenes is a genetically heterogeneous species, which is divided into evolutionary lineages and clonal complexes (CCs). Not all L. monocytogenes isolates are equally likely to cause disease, with CC1, and in particular sequence type (ST) 1, being the most prevalent complex in human and ruminant i...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000529

    authors: Rupp S,Bärtschi M,Frey J,Oevermann A

    更新日期:2017-07-01 00:00:00

  • Establishment of a Rep' protein antibody detection method to distinguish natural infection with PCV2 from subunit vaccine immunization.

    abstract::Introduction. PCV2 is a DNA virus that exists widely in pigs and has caused great economic losses to the pig industry worldwide. In the existing commercial PCV2 enzyme-linked immunosorbent assay (ELISA) kits both natural infection with PCV2 and vaccine immunization produce results that are positive for PCV2 Cap antibo...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.001230

    authors: Chen Q,Rong J,Li G,Xu B,Wang X,Hu J,Rong M,Li H

    更新日期:2020-09-01 00:00:00

  • In-vitro inhibition of mycobacteria by viridans streptococci.

    abstract::Eleven strains of Mycobacterium tuberculosis and 13 other species of mycobacteria were examined for susceptibility to 26 strains of viridans streptococci. Susceptibility was detected by the simultaneous antagonism technique, but not by tests of deferred antagonism. It is concluded that in-vitro inhibition of mycobacte...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-19-2-227

    authors: Allen BW

    更新日期:1985-04-01 00:00:00

  • Adhesion of fimbriate Escherichia coli to bovine mammary-gland epithelial cells in vitro.

    abstract::The adhesion of bovine and a human isolate of Escherichia coli to epithelial cells from the teat and lactiferous sinuses of the udder was examined. Adhesion was detected with bacterial suspensions that produced mannose-sensitive agglutination of guinea-pig red cells. Adhesion to epithelial cells could be inhibited by ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-11-2-117

    authors: Harper M,Turvey A,Bramley AJ

    更新日期:1978-05-01 00:00:00

  • Assessment of possible tuberculous lymphadenopathy by PCR compared to non-molecular methods.

    abstract::Tuberculosis is a major public health problem in India and other developing countries and has formed a lethal partnership with AIDS. It often presents a diagnostic challenge especially when clinical presentation is suggestive but bacteriological proof is lacking. The objective of this study was to compare the various ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.45904-0

    authors: Pahwa R,Hedau S,Jain S,Jain N,Arora VM,Kumar N,Das BC

    更新日期:2005-09-01 00:00:00

  • Epidemiological, biochemical and antimicrobial susceptibility characteristics of Streptococcus pseudoporcinus isolated in Quebec, Canada, from 1997 to 2006.

    abstract::From 1997 to 2006, in the province of Quebec, Canada, 15 isolates of Streptococcus pseudoporcinus from 1 urine and 14 vaginorectal cultures were recovered from the genitourinary tract of pregnant women. All these women originated from the Caribbean or sub-Saharan Africa (P=0.00045 compared with a suitable control grou...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.47295-0

    authors: Gaudreau C,Simoneau E,Labrecque O,Laurence RA,Laferrière C,Miller M,Raynal L,Rallu F

    更新日期:2007-12-01 00:00:00

  • Effects of altering the germination potential of Bacillus anthracis spores by exogenous means in a mouse model.

    abstract::Inhalational anthrax is the most severe form of anthrax. It has been shown in small-animal and non-human primate models that relatively large pools of ungerminated Bacillus anthracis spores can remain within the alveolar spaces for days to weeks post-inhalation or until transported to areas more favourable for germina...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.008656-0

    authors: Cote CK,Bozue J,Twenhafel N,Welkos SL

    更新日期:2009-06-01 00:00:00

  • Cryptococcus neoformans induces alterations in the cytoskeleton of human brain microvascular endothelial cells.

    abstract::The fungal pathogen Cryptococcus neoformans has a predilection for the central nervous system (CNS), resulting in devastating meningoencephalitis. At present, it is unclear how C. neoformans traverses the blood-brain barrier (BBB) and causes CNS infection. The present study has examined and characterized the interacti...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.05230-0

    authors: Chen SHM,Stins MF,Huang SH,Chen YH,Kwon-Chung KJ,Chang Y,Kim KS,Suzuki K,Jong AY

    更新日期:2003-11-01 00:00:00

  • Role of caspases in cleavage of lamin A/C and PARP during apoptosis in macrophages infected with a periodontopathic bacterium.

    abstract::The periodontopathic bacterium Actinobacillus actinomycetemcomitans has been implicated in the pathogenesis of periodontal diseases. It has been reported previously that infection with the organism induced apoptosis in the mouse macrophage cell line J774.1. In the present study, the role of caspases during apoptosis i...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.47193-0

    authors: Okinaga T,Kasai H,Tsujisawa T,Nishihara T

    更新日期:2007-10-01 00:00:00

  • Maternofetal infections due to Eikenella corrodens.

    abstract::Eikenella corrodens, a commensal of the human oral cavity, is generally associated with bite wounds and head and neck infections. Neonatal infections are rare. We report two cases of premature birth associated with maternofetal E. corrodens infection. ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.002568-0

    authors: Garnier F,Masson G,Bedu A,Masson P,Decroisette E,Guigonis V,Fermeaux V,De Barbentane MC,Denis F,Ploy MC

    更新日期:2009-02-01 00:00:00

  • Bacteroides ureolyticus (NTU) medium for the selective recovery of Bacteroides gracilis.

    abstract::Bacteroides gracilis is a gram-negative anaerobic bacillus which requires formate and fumarate for growth; it has been implicated in periodontal disease and serious infections of the head and neck. In this study, Bacteroides ureolyticus (NTU) medium was tested for its ability to allow the growth of B. gracilis and oth...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-35-5-294

    authors: Wade WG,Bishop PA,Slayne MA

    更新日期:1991-11-01 00:00:00

  • Molecular epidemiology of Bordetella pertussis in Greece, 2010-2015.

    abstract:PURPOSE:To determine the predominant strains of Bordetella pertussis in Greece during 2010-2015. METHODOLOGY:Infants and children (n=1150) (15 days to 14 years) of Greek, Roma and immigrant origin with different vaccination statuses were hospitalized in Athens, Greece with suspected pertussis infection. IS481/IS1001 r...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000688

    authors: Petridou E,Jensen CB,Arvanitidis A,Giannaki-Psinaki M,Michos A,Krogfelt KA,Petersen RF

    更新日期:2018-03-01 00:00:00

  • Pseudomonas aeruginosa fosfomycin resistance mechanisms affect non-inherited fluoroquinolone tolerance.

    abstract::Pseudomonas aeruginosa is an opportunistic pathogen that poses a threat in clinical settings due to its intrinsic and acquired resistance to a wide spectrum of antibiotics. Additionally, the presence of a subpopulation of cells surviving high concentrations of antibiotics, called persisters, makes it virtually impossi...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.019703-0

    authors: De Groote VN,Fauvart M,Kint CI,Verstraeten N,Jans A,Cornelis P,Michiels J

    更新日期:2011-03-01 00:00:00

  • Antibiotic-induced release of endotoxin from bacteria in vitro.

    abstract::The ability of cefotaxime, ciprofloxacin, piperacillin and tobramycin to cause release of endotoxin was examined in vitro with cultures of Enterobacter cloacae and Escherichia coli. Endotoxin was measured by a quantitative limulus amoebocyte lysate assay and its presence was confirmed by silver staining of the lipopol...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-40-1-23

    authors: Crosby HA,Bion JF,Penn CW,Elliott TS

    更新日期:1994-01-01 00:00:00

  • Streptococcus mutans infective endocarditis complicated by vertebral discitis following dental treatment without antibiotic prophylaxis.

    abstract::We report what we believe is the first reported case of Streptococcus mutans endocarditis complicated by vertebral discitis. The case is particularly interesting and topical as it occurred in a patient with pre-existing cardiac valvular disease who had recently had a dental procedure without antibiotic prophylaxis fol...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.020974-0

    authors: Biswas S,Bowler ICJW,Bunch C,Prendergast B,Webster DP

    更新日期:2010-10-01 00:00:00

  • Campylobacters and impaired fetal development in mice.

    abstract::Intravenous injection of eight human strains of Campylobacter fetus ss fetus and Campylobacter jejuni into mice at various stages of pregnancy demonstrated significant strain differences in ability to affect implantation of the fertilised ovum and to cause resorption of the mouse fetus. Implantation was significantly ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-25-1-7

    authors: O'Sullivan AM,Doré CJ,Coid CR

    更新日期:1988-01-01 00:00:00

  • Phagocytosis and ingestion of influenza virus by human polymorphonuclear leucocytes in vitro: electronmicroscopy studies.

    abstract::Interaction of human polymorphonuclear leucocytes (PMNL) and influenza virus (IFV) was studied in vitro. At 0 degree C, the viral particles were bound extensively to the surface of the PMNL membrane with a ratio of about 1000 virus particles to a single PMNL. The binding was sensitive to neuraminidase, suggesting atta...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-28-3-191

    authors: Yamamoto K,Suzuki K,Suzuki K,Mizuno S

    更新日期:1989-03-01 00:00:00

  • Denaturing gradient gel electrophoresis analysis for the detection of point mutations in the Chlamydia trachomatis major outer-membrane protein gene.

    abstract::Fifty clinical strains of Chlamydia trachomatis were studied by denaturing gradient gel electrophoresis (DGGE) of bacterial DNA amplified by the polymerase chain reaction (PCR). The strains belonged to the three most commonly encountered serovars in developed countries--D, E and F. Six reference strains, including the...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-43-1-14

    authors: Sayada C,Denamur E,Grandchamp B,Orfila J,Elion J

    更新日期:1995-07-01 00:00:00

  • Isolation of a gastric campylobacter-like organism from the stomach of four rhesus monkeys, and identification as Campylobacter pylori.

    abstract::Campylobacter-like organisms, isolated from the gastric antrum of Rhesus monkeys, were compared with Campylobacter jejuni and C. pylori. They were similar to C. pylori by light microscopy, in ultrastructural morphology, in enzymic, fatty-acid-methyl-ester, and protein-profile analysis, and in antigenic reactivity with...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-27-1-41

    authors: Newell DG,Hudson MJ,Baskerville A

    更新日期:1988-09-01 00:00:00

  • Listeria monocytogenes cells under nutrient deprivation showed reduced ability to infect the human intestinal cell line HT-29.

    abstract:PURPOSE:This study aimed to evaluate the effect of two types of stress, cold and nutritional, on the viability and the in vitro virulence of the foodborne pathogenic bacteria Listeria monocytogenes. METHODOLOGY:Ten diverse isolates were kept in phosphate-buffered saline (PBS) at optimal (37 °C) or at refrigeration tem...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000648

    authors: Araujo V,Neves E,Silva AC,Martins APL,Brito LC

    更新日期:2018-01-01 00:00:00

  • A rationale for using steroids in the treatment of severe cases of H5N1 avian influenza.

    abstract::Acute hypercytokinaemia represents an imbalance of pro-inflammatory and anti-inflammatory cytokines, and is believed to be responsible for the development of acute respiratory distress syndrome and multiple organ failure in severe cases of avian (H5N1) influenza. Although neuraminidase inhibitors are effective in trea...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章,评审

    doi:10.1099/jmm.0.47124-0

    authors: Carter MJ

    更新日期:2007-07-01 00:00:00

  • Inactivating effects of the lactoperoxidase system on bacterial lyases involved in oral malodour production.

    abstract::The main components of oral malodour have been identified as volatile sulfur compounds (VSCs), including hydrogen sulfide (H(2)S) and methyl mercaptan (CH(3)SH). The lactoperoxidase (LPO) system (consisting of LPO, glucose oxidase, glucose and thiocyanate) was previously shown to exhibit antimicrobial activities again...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000150

    authors: Nakano M,Shin K,Wakabayashi H,Yamauchi K,Abe F,Hironaka S

    更新日期:2015-10-01 00:00:00

  • In vitro assessment of the APTIMA Combo 2 assay for the detection of Chlamydia trachomatis using highly purified elementary bodies.

    abstract::The Gen-Probe APTIMA Combo 2 assay has previously been reported to have a high sensitivity. The end point of this assay was evaluated using highly purified chlamydial elementary bodies (EBs). The performance of the APTIMA Combo 2 assay was compared with a commercially available PCR kit, AMPLICOR Chlamydia trachomatis....

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.45911-0

    authors: Ikeda-Dantsuji Y,Konomi I,Nagayama A

    更新日期:2005-04-01 00:00:00

  • Replication of Chlamydia trachomatis and C. pneumoniae in the human monocytic cell line U-937.

    abstract::To elucidate whether Chlamydia trachomatis and C. pneumoniae infections occur to a significant extent in monocytes-macrophages, the human monocytic cell line, U-937, was infected with C. trachomatis L2 or C. pneumoniae TW-183. Chlamydial DNA and genus-specific antigens of the lipopolysaccharides (LPS) in epitopes of t...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-42-3-191

    authors: Numazaki K,Suzuki K,Chiba S

    更新日期:1995-03-01 00:00:00