Mapping the genetic diversity within major clonal complexes of meticillin-resistant Staphylococcus aureus utilizing genome-wide fluorescent amplified fragment length polymorphism markers.

Abstract:

:The genetic diversity between major meticillin-resistant Staphylococcus aureus (MRSA) lineages was probed using fluorescent amplified fragment length polymorphism (FAFLP) as a random genome sampling tool. Genomic DNA was digested with endonucleases BglII and Csp6I and a subset of the restricted fragments were amplified using the primer pair BglII+A and Csp6I+0. Sixty-seven FAFLP profiles consisting of 46-68 amplified fragments ranging in size from 50 to 600 bp were exhibited amongst the 71 isolates analysed. Cluster analysis of FAFLP data revealed concordance with spa typing and MLST clonal complexes (CC), with isolates of each CC grouping in the same FAFLP cluster. Furthermore, FAFLP could differentiate subtypes within the homogeneous CC22 isolates and also between MLST sequence types 8 and 239. The discriminatory power of FAFLP was 0.998 compared to values of 0.975 and 0.909 for spa typing and MLST, respectively. Thus, FAFLP analysis proved to be a rapid, reproducible and high-resolution tool that displayed the microheterogeneity within MRSA lineages. Using FAFLP data, lineage-specific fragments were identified and sequenced; these encoded toxins, antibiotic resistance determinants and bacteriophage resistance factors. Lineage-specific sequence variations were observed, which may provide insights into the evolution and fitness of successful lineages. This will also aid in the development of rapid and high-throughput diagnostic PCR-based assays for the identification of MRSA lineages in resource-poor settings.

journal_name

J Med Microbiol

authors

Desai D,Ellington MJ,Arnold C,Desai M

doi

10.1099/jmm.0.049429-0

subject

Has Abstract

pub_date

2012-12-01 00:00:00

pages

1673-1680

issue

Pt 12

eissn

0022-2615

issn

1473-5644

journal_volume

61

pub_type

杂志文章
  • Virulence of an emerging respiratory pathogen, genus Pandoraea, in vivo and its interactions with lung epithelial cells.

    abstract::Pandoraea species have emerged as opportunistic pathogens among cystic fibrosis (CF) and non-CF patients. Pandoraea pulmonicola is the predominant Pandoraea species among Irish CF patients. The objective of this study was to investigate the pathogenicity and potential mechanisms of virulence of Irish P. pulmonicola is...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.022657-0

    authors: Costello A,Herbert G,Fabunmi L,Schaffer K,Kavanagh KA,Caraher EM,Callaghan M,McClean S

    更新日期:2011-03-01 00:00:00

  • Isolation and characterisation of a novel spirochaete from severe virulent ovine foot rot.

    abstract::A novel spirochaete was isolated from a case of severe virulent ovine foot rot (SVOFR) by immunomagnetic separation with beads coated with polyclonal anti-treponemal antisera and prolonged anaerobic broth culture. The as yet unnamed treponeme differs considerably from the only other spirochaete isolated from ovine foo...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-50-12-1061

    authors: Demirkan I,Carter SD,Winstanley C,Bruce KD,McNAIR NM,Woodside M,Hart CA

    更新日期:2001-12-01 00:00:00

  • Nosocomial infection with Asaia lannensis in two paediatric patients with idiopathic dilated cardiomyopathy.

    abstract::This is the first report, to our knowledge, of two temporally and geographically related nosocomial Asaia lannensis infections in a paediatric setting. Two patients with idiopathic dilated cardiomyopathy awaiting cardiac transplantation developed bacteraemia during their hospital stay. The physical location of both pa...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.019067-0

    authors: Juretschko S,Beavers-May TK,Stovall SH

    更新日期:2010-07-01 00:00:00

  • Prevalence, risk factors and molecular epidemiology of Brachyspira pilosicoli in humans on the island of Bali, Indonesia.

    abstract::The purpose of this study was to investigate the prevalence and epidemiology of the anaerobic intestinal spirochaete Brachyspira pilosicoli amongst Indonesians living in rural and urban settings on the island of Bali. Faecal samples (n = 992) were collected on two occasions, 4 months apart, from people living in four ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.05415-0

    authors: Margawani KR,Robertson ID,Brooke CJ,Hampson DJ

    更新日期:2004-04-01 00:00:00

  • An improved selective medium for the isolation of Escherichia coli O157.

    abstract::Sorbitol-MacConkey medium has become widely used for the isolation of verotoxigenic (VT+) Escherichia coli O157. However, many organisms other than VT+ E. coli O157, especially other serogroups of E. coli and Proteus spp., may not ferment sorbitol, and thus may be confused initially with VT+ E. coli O157. Rhamnose is ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-35-2-107

    authors: Chapman PA,Siddons CA,Zadik PM,Jewes L

    更新日期:1991-08-01 00:00:00

  • The effects of iclaprim on exotoxin production in methicillin-resistant and vancomycin-intermediate Staphylococcus aureus.

    abstract:PURPOSE:Extracellular protein toxins contribute to the pathogenesis of Staphylococcus aureus infections. The present study compared the effects of iclaprim and trimethoprim - two folic acid synthesis inhibitors - with nafcillin and vancomycin on production of Panton-Valentine leukocidin (PVL), alpha haemolysin (AH) and...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000929

    authors: Bryant AE,Gomi S,Katahira E,Huang DB,Stevens DL

    更新日期:2019-03-01 00:00:00

  • Effect of passive immunotherapy on murine gut-derived sepsis caused by Pseudomonas aeruginosa.

    abstract::The effect of passive immunotherapy with antisera against heat-killed Pseudomonas aeruginosa and three of its exo-enzymes (elastase, alkaline protease and exotoxin A) in gut-derived P. aeruginosa sepsis was evaluated. Mice were given a suspension of P. aeruginosa strain D4 in their drinking water, together with ampici...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-48-8-765

    authors: Matsumoto T,Furuya N,Tateda K,Miyazaki S,Ohno A,Ishii Y,Hirakata Y,Yamaguchi K

    更新日期:1999-08-01 00:00:00

  • A collaborative evaluation of a rapid, semi-automated identification system for gram-negative bacilli: the Quantum II BID.

    abstract::The recently-introduced semi-automated bacterial identification system, the Quantum II BID, is designed to identify fermentative and non-fermentative gram-negative bacilli in 4-5 h. The system was evaluated independently by the two participating laboratories. Inter-laboratory reproducibility was determined by testing ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-21-2-145

    authors: Stevens M,Henrichsen C,Smith M

    更新日期:1986-03-01 00:00:00

  • Multidrug-resistant organisms in a routine ward environment: differential propensity for environmental dissemination and implications for infection control.

    abstract::Multidrug-resistant organisms (MDROs) pose significant infection-control challenges in settings with high prevalence and limited isolation facilities. This observational study in an 800-bed hospital determined the prevalence, bacterial density and genetic relatedness of MDROs isolated from ward surfaces, medical devic...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.052860-0

    authors: Tan TY,Tan JSM,Tay H,Chua GH,Ng LSY,Syahidah N

    更新日期:2013-05-01 00:00:00

  • Detection of a novel bunyavirus associated with fever, thrombocytopenia and leukopenia syndrome in Henan Province, China, using real-time reverse transcription PCR.

    abstract::A novel bunyavirus associated with fever, thrombocytopenia and leukopenia syndrome (FTLS) was discovered in Henan Province, China. Here, we report the development of an assay for this novel bunyavirus based on real-time reverse transcription PCR (RT-PCR). The assay exhibited high sensitivity and specificity without cr...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.049577-0

    authors: Huang X,Liu L,Du Y,Ma H,Mu Y,Pengzhi Wang,Ma H,Tang X,Wang H,Kang K,Zhang S,Wu W,Chen H,Liu G,Yang Y,Yongqiangj,Jiang,Xu B,Chen W

    更新日期:2013-07-01 00:00:00

  • Types of Salmonella paratyphi B and their phylogenetic significance.

    abstract::The substrates inositol, rhamnose, d-tartrate and m-tartrate used in fermentation tests with 338 cultures of Salmonella paratyphi B differentiated strains in some phage types to give information that could be used in epidemiological investigations. Xylose in Bitter's medium, the fifth substrate by which 13 of a potent...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-26-4-285

    authors: Barker RM,Kearney GM,Nicholson P,Blair AL,Porter RC,Crichton PB

    更新日期:1988-08-01 00:00:00

  • Adhesion of clinical and environmental Aeromonas isolates to HEp-2 cells.

    abstract::A total of 63 Aeromonas strains isolated from diarrhoeal faeces or water samples were tested for adhesion to HEp-2 cells. An association between diarrhoea and high level adhesion was observed in that 12 of the 34 faecal isolates and none of the 29 environmental isolates yielded greater than 20 bacteria per HEp-2 cell ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-26-1-19

    authors: Carrello A,Silburn KA,Budden JR,Chang BJ

    更新日期:1988-05-01 00:00:00

  • Pneumocystis carinii carriage in immunocompromised patients with and without human immunodeficiency virus infection.

    abstract::Eighty-one bronchoalveolar lavage (BAL) specimens obtained from 26 HIV-infected, 45 non-HIV immunosuppressed and 10 immunocompetent patients with primary pulmonary diseases were analysed for the presence of Pneumocystis carinii by staining and by P. carinii 5S rDNA determined by PCR. P. carinii was observed by stainin...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-51-7-611

    authors: Takahashi T,Goto M,Endo T,Nakamura T,Yusa N,Sato N,Iwamoto A

    更新日期:2002-07-01 00:00:00

  • Factors affecting the adhesion of Candida albicans to epithelial cells of insulin-using diabetes mellitus patients.

    abstract::This study investigated the influence of the carbon source of the growth medium, strains of Candida albicans and source of epithelial cells, and the influence of smoking and gender, on the adhesion of C. albicans to epithelial cells from insulin-using diabetic patients. Adhesion was determined by an autologous adhesio...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-49-3-291

    authors: Willis AM,Coulter WA,Hayes JR,Bell P,Lamey PJ

    更新日期:2000-03-01 00:00:00

  • Synergistic potential of Juniperus communis and Helichrysum italicum essential oils against nontuberculous mycobacteria.

    abstract:OBJECTIVE:The present study evaluated the possible synergistic antimycobacterial interactions of Juniperus communis and Helichrysum italicum essential oils (EO). METHODS:Antimycobacterial potential was tested against Mycobacterium avium and Mycobacterium intracellulare using broth and water dilution method and checker...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000962

    authors: Peruč D,Tićac B,Abram M,Broznić D,Štifter S,Staver MM,Gobin I

    更新日期:2019-05-01 00:00:00

  • Biotypes of Haemophilus parainfluenzae from the respiratory secretions in chronic bronchitis.

    abstract::A total of 2401 isolates of Haemophilus parainfluenzae was isolated from respiratory secretions of 36 healthy adults and 128 patients with chronic bronchitis over a period of 1 year. The isolates were allocated to eight biotypes, by their production of indole, urease and ornithine decarboxylase. Biotypes I and II cons...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-36-4-279

    authors: Taylor DC,Cripps AW,Clancy RL,Murree-Allen K,Hensley MJ,Saunders NA,Sutherland DC

    更新日期:1992-04-01 00:00:00

  • Effect of EDTA on the resistance of clinical isolates of Acinetobacter baumannii to the bactericidal activity of normal human serum.

    abstract::Acinetobacter baumannii is an opportunist nosocomial pathogen of world-wide importance and produces severe infections in immunocompromised patients. However, the virulence factors contributing to its pathogenic properties are not well known. The effect of normal human serum against 18 clinical isolates of the most pre...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-49-11-1047

    authors: García A,Solar H,González C,Zemelman R

    更新日期:2000-11-01 00:00:00

  • Emergence of OXA-carbapenemase- and 16S rRNA methylase-producing international clones of Acinetobacter baumannii in Norway.

    abstract::This study was designed to investigate the molecular epidemiology and antibiotic-resistance characteristics of 11 carbapenem-resistant clinical isolates of Acinetobacter baumannii obtained in Norway between 2004 and 2009. Interestingly, all the isolates were linked with recent hospitalization outside Norway. The epide...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.028340-0

    authors: Karah N,Haldorsen B,Hermansen NO,Tveten Y,Ragnhildstveit E,Skutlaberg DH,Tofteland S,Sundsfjord A,Samuelsen Ø

    更新日期:2011-04-01 00:00:00

  • Incidence of common pyocin types of Pseudomonas aeruginosa from patients with cystic fibrosis and chronic airways diseases.

    abstract::We sought evidence to determine if particular strains of Pseudomonas aeruginosa have a predilection for pulmonary colonisation in patients with cystic fibrosis (CF). The incidence of common pyocin types in non-CF isolates (74%) was similar to that noted in previous reports but differed significantly (X2 = 16.7, p less...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-32-3-169

    authors: Tredgett MW,Doherty C,Govan JR

    更新日期:1990-07-01 00:00:00

  • Nuclear bilateral Bell's palsy and ageusia associated with Mycoplasma pneumoniae pulmonary infection.

    abstract::This case report describes a case of nuclear bilateral Bell's palsy and ageusia associated with Mycoplasma pneumoniae infection. Magnetic resonance imaging evidenced T2-weighted hyper-intense protuberantial lesions. Such topography leading to a nuclear palsy contrasts with previously reported infectious diplegia invol...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.45859-0

    authors: Trad S,Ghosn J,Dormont D,Stankoff B,Bricaire F,Caumes E

    更新日期:2005-04-01 00:00:00

  • PCR evaluation of false-positive signals from two automated blood-culture systems.

    abstract::Rapid detection of micro-organisms from blood is one of the most critical functions of a diagnostic microbiology laboratory. Automated blood-culture systems reduce the time needed to detect positive cultures, and reduce specimen handling. The false-positive rate of such systems is 1-10%. In this study, the presence of...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.46196-0

    authors: Karahan ZC,Mumcuoglu I,Guriz H,Tamer D,Balaban N,Aysev D,Akar N

    更新日期:2006-01-01 00:00:00

  • Cronobacter sakazakii induces necrotizing enterocolitis by regulating NLRP3 inflammasome expression via TLR4.

    abstract::Introduction. Neonatal infection with Cronobacter sakazakii can cause severe intestinal damage and necrotizing enterocolitis (NEC). The inflammasome and Toll-like receptors mediate intestinal damage caused by other intestinal pathogens causing NEC, but the exact mechanism is unclear.Aim. We evaluated the molecular mec...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.001181

    authors: Chen Z,Zhang Y,Lin R,Meng X,Zhao W,Shen W,Fan H

    更新日期:2020-05-01 00:00:00

  • Evaluation of ResistancePlus MG FleXible, a 'near-patient' test for the detection of Mycoplasma genitalium and macrolide resistance mutations, using freshly collected clinical samples.

    abstract::Introduction.Mycoplasma genitalium is a sexually transmitted pathogen with increasing resistance to first- and second-line antimicrobials. The 'near-patient test' ResistancePlus MG FleXible (SpeeDx) detects M. genitalium plus four macrolide resistance mutations (MRMs), facilitating same-day patient follow up.Hypothesi...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.001271

    authors: Murray GL,Doyle M,Bodiyabadu K,Vodstrcil LA,Garland SM,Danielewski J,Machalek DA,McGuinness C,Plummer EL,De Petra V,Williamson DA,Bradshaw CS

    更新日期:2021-01-01 00:00:00

  • Immunological response to a strain of Staphylococcus epidermidis in the rabbit: production of protective antibody.

    abstract::A rabbit was immunised with a heat-killed vaccine prepared from strain 1142 of Staphylococcus epidermidis. The HA titres of the antisera against a surface-polysaccharide antigen extracted from the homologous strain were highest in the 2nd-4th weeks after immunisation and subsequently declined. Only a minor amount of 2...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-11-4-371

    authors: Yoshida K,Ichiman Y

    更新日期:1978-11-01 00:00:00

  • The special affinity of particular types of Proteus mirabilis for the urinary tract.

    abstract::The strains of Proteus species found in significant numbers and as pure cultures in urine from 217 individuals were isolated, identified to species level and typed for proticine production (P type) and proticine sensitivity (S type) to give their P/S type. Urinary-tract infections with Proteus, principally P. mirabili...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-12-1-1

    authors: Senior BW

    更新日期:1979-02-01 00:00:00

  • An in vitro and in vivo study on the synergistic effect and mechanism of itraconazole or voriconazole alone and in combination with tetrandrine against Aspergillus fumigatus.

    abstract::In this study, we investigated the in vitro antifungal effects of itraconazole/voriconazole (ITR/VRC) alone and in combination with tetrandrine (TET) against 23 clinical isolates of A. fumigatus using a chequerboard microdilution method. The dynamic antifungal effects of TET with ITR/VRC against A. fumigatus were asse...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000120

    authors: Li SX,Song YJ,Zhang LL,Shi JP,Ma ZL,Guo H,Dong HY,Li YM,Zhang H

    更新日期:2015-09-01 00:00:00

  • Hepatitis C virus genotyping in relation to neu-oncoprotein overexpression and the development of hepatocellular carcinoma.

    abstract::The distribution of hepatitis C virus (HCV) genotypes among Egyptian patients positive for anti-HCV was determined and their influence, when combined with neu-oncoprotein overexpression, on the development of hepatocellular carcinoma (HCC) was examined. The study groups included asymptomatic carriers (ASC) and patient...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-49-1-89

    authors: Zekri AN,Bahnassy AA,Shaarawy SM,Mansour OA,Maduar MA,Khaled HM,El-Ahmadi O

    更新日期:2000-01-01 00:00:00

  • Characteristics of cysteine-requiring strains of Klebsiella isolated from urinary tract infections.

    abstract::Clinical and bacteriological findings in seven cases of urinary tract infection with cysteine-requiring strains of Klebsiella are described. The organisms were isolated from patients with long-standing urinary tract abnormalities and grew as small (c. 1 mm) colonies on MacConkey agar. The organisms failed to grow in a...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-26-3-211

    authors: McIver CJ,Tapsall JW

    更新日期:1988-07-01 00:00:00

  • Verocytotoxin-producing Escherichia coli (VTEC) O157 and other VTEC from human infections in England and Wales: 1995-1998.

    abstract::A total of 3429 isolations of verocytotoxin-producing Escherichia coli O157 (VTEC O157) was confirmed from human sources in England and Wales during the period 1995-1998. The largest annual total was 1087 in 1997. Most infections occurred in the third quarter of each year. The overall rate of infection ranged from 1.2...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-50-2-135

    authors: Willshaw GA,Cheasty T,Smith HR,O'Brien SJ,Adak GK

    更新日期:2001-02-01 00:00:00

  • Characteristics of antibiotic-resistant Escherichia coli in the rectum of healthy school-children.

    abstract::During a 3-year period, 771 rectal swabs were taken from abacteriuric school-children. Out of 709 E. coli strains, each isolated from one faecal specimen, 102 were found to be resistant to one or more antibacterial agents, and 607 to be fully sensitive. Another 204 resistant strains were found by selection for antibio...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-10-3-299

    authors: Lidin-Janson G,Falsen E,Jodal U,Kaijser B,Lincoln K

    更新日期:1977-08-01 00:00:00