Abstract:
:This study was designed to investigate the molecular epidemiology and antibiotic-resistance characteristics of 11 carbapenem-resistant clinical isolates of Acinetobacter baumannii obtained in Norway between 2004 and 2009. Interestingly, all the isolates were linked with recent hospitalization outside Norway. The epidemiological status was investigated by multilocus sequence typing (MLST), multiplex PCR assays for major international clones, typing of blaOXA-51-like variants and PFGE. The genotypic-resistance characteristics, including the occurrence of OXA-carbapenemase-encoding and 16S rRNA methylase-encoding genes and class 1 integrons, were investigated by PCR assays and sequencing. Seven isolates were found to harbour blaOXA-66 and belong to MLST clonal complexes (CCs) CC2P (Pasteur Institute scheme) and CC92B (Bartual scheme), and international clone II. One isolate harboured blaOXA-69, and belonged to CC1P, CC109B and international clone I. Two isolates belonged to sequence group 9, probably a subgroup of international clone I, and one isolate belonged to sequence group 4, a proposed novel international clone. All isolates contained an acquired OXA-carbapenemase-encoding gene: blaOXA-23-like (n=9), blaOXA-24-like (n=1) and blaOXA-58-like (n=1). Four isolates with high-level aminoglycoside-resistance contained the 16S rRNA methylase-encoding armA gene. Class 1 integrons with six different variable regions were detected. Sequence analysis of gene cassettes identified four aminoglycoside (aacA4, aac(6')-Im, aadA1 and aacC1), two chloramphenicol (catB8 and cm1A5), one β-lactamase (blaOXA-20) and one rifampicin (arr-2) resistance gene in various combinations. In conclusion, the occurrence of A. baumannii isolates producing OXA carbapenemase and 16S rRNA methylase in Norway was related to the worldwide distribution of international clones I and II, and the emergence of novel international clones.
journal_name
J Med Microbioljournal_title
Journal of medical microbiologyauthors
Karah N,Haldorsen B,Hermansen NO,Tveten Y,Ragnhildstveit E,Skutlaberg DH,Tofteland S,Sundsfjord A,Samuelsen Ødoi
10.1099/jmm.0.028340-0subject
Has Abstractpub_date
2011-04-01 00:00:00pages
515-521issue
Pt 4eissn
0022-2615issn
1473-5644journal_volume
60pub_type
杂志文章abstract::Experiments were performed to determine whether a modern flow cytometer could be used to study bacterial populations in suspension, with particular reference to their morphological characteristics and their responses to antibiotics. The FACScan, a commercial benchtop flow cytometer fitted with an air-cooled laser, des...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-39-2-147
更新日期:1993-08-01 00:00:00
abstract::Small numbers of bacteria capable of growing on agar supplemented with amoxycillin 40 mg/L were isolated from the saliva of 9 out of 20 adult volunteers in a previous study. All the bacteria were identified as Streptococcus sanguis although no strains produced dextran in conventional tests. However, using a specific a...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-24-3-259
更新日期:1987-11-01 00:00:00
abstract::Clostridium difficile is a frequent cause of severe, recurrent, post-antibiotic diarrhoea and pseudomembranous colitis. Its pathogenicity is mediated mainly by two toxins, TcdA and TcdB. However, different adhesins have also been described as important colonization factors which are implicated in the first step of the...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.029553-0
更新日期:2011-08-01 00:00:00
abstract::An outer-membrane protein (OMP) was isolated from a clinical strain of Bacteroides distasonis. Changes in growth media did not appreciably affect the appearance of this protein in crude outer-membrane preparations examined by SDS-PAGE. However, the proportion of the protein relative to other OMPs was greater in 24-h c...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-37-3-165
更新日期:1992-09-01 00:00:00
abstract::Staphylococcus aureus is a leading aetiologic agent of nosocomial- and community-acquired infectious diseases worldwide. The public health concern regarding staphylococcal infections is inflated by the increasing occurrence of multidrug-resistant strains, e.g. multidrug- and meticillin-resistant S.aureus (MDR MRSA). T...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000343
更新日期:2016-10-01 00:00:00
abstract::The rapid identification of pathogenic yeasts is a crucial step in ensuring that effective antifungal treatment is started as early as possible. CandiSelect 4 (CS4; Bio-Rad) is a new chromogenic medium for the isolation of fungi, the direct identification of Candida albicans and the presumptive identification of the m...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.46715-0
更新日期:2007-04-01 00:00:00
abstract::A novel direct detection system has been developed for eight staphylococcal enterotoxin (SE)-encoding genes (sea, seb, sec, sed, see, seg, seh and sei) in milk. Specific detection by real-time PCR was successful for all SE-encoding genes in the reference strains. Furthermore, a novel DNA-preparation method with good r...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.46027-0
更新日期:2006-03-01 00:00:00
abstract::Episodes of cholera stemming from indigenous Vibrio cholerae strains in Australia are mainly associated with environmental sources. In the present study, 10 V. cholerae O1 strains of Australian origin were characterized. All of the strains were serogroup O1 and their conventional phenotypic traits categorized them as ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.004333-0
更新日期:2009-01-01 00:00:00
abstract::The main components of oral malodour have been identified as volatile sulfur compounds (VSCs), including hydrogen sulfide (H(2)S) and methyl mercaptan (CH(3)SH). The lactoperoxidase (LPO) system (consisting of LPO, glucose oxidase, glucose and thiocyanate) was previously shown to exhibit antimicrobial activities again...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000150
更新日期:2015-10-01 00:00:00
abstract::This study analysed the characteristics and genetic similarity of recent Klebsiella pneumoniae carbapenemase (KPC-2)-producing Klebsiella pneumoniae isolates from Korea. Recent laboratory surveillance detected an increase in carbapenemase-producing Enterobacteriaceae in Korea. A total of 6 KPC-2-producing K. pneumonia...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.059428-0
更新日期:2013-09-01 00:00:00
abstract::The purpose of this study was to verify whether the presence of any of the Helicobacter pylori cagPAI genes or segments--cagA, cagA promoter, cagE, cagM, tnpB, tnpA, cagT and the left end of the cag II (LEC) region--would be a useful marker for the risk of peptic ulcer disease development. H. pylori DNA extracted from...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.46824-0
更新日期:2007-01-01 00:00:00
abstract::Some strains of Helicobacter pylori are known to produce an extracellular cytotoxin that causes vacuolation in cultured mammalian cells. Screening for such strains makes use of HeLa cells which may not be sensitive enough to detect minimal changes. The aim of this study was to develop a more sensitive cell line. Vacuo...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-46-8-705
更新日期:1997-08-01 00:00:00
abstract::The identity and motility of enterococci isolated as urinary pathogens were compared with those of strains from other sources. The majority of isolates from all sources proved to be Streptococcus faecalis of S. faecium but eight of 117 urinary strains and seven of 127 non-urinary strains belonged to a group intermedia...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-15-1-127
更新日期:1982-02-01 00:00:00
abstract::Helicobacter pylori is classified as a class I carcinogenic factor and its persistent colonization in the stomach induces gastric cancer. Cancerous Inhibitor of PP2A (CIP2A) is a newly identified oncoprotein overexpressed in gastric cancer. Serving as a key oncoprotein, CIP2A also participates in regulation of senesce...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.014704-0
更新日期:2010-03-01 00:00:00
abstract::Amplification and sequence analysis of the 16S rRNA gene can be applied to detect and identify bacteria in clinical samples. We examined 75 clinical samples (17 culture-positive, 58 culture-negative) prospectively by two different PCR protocols, amplifying either a single fragment (1343 bp) or two fragments (762/598 b...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.030387-0
更新日期:2012-04-01 00:00:00
abstract::Herein we describe a real-time quantitative PCR assay for evaluating the adherence of Clostridium difficile to differentiated human intestinal Caco-2 cells. Our investigations demonstrated that the method, employing the C. difficile-specific triose-phosphate isomerase gene, is as reliable but less time-consuming than ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.019752-0
更新日期:2010-08-01 00:00:00
abstract::In 2002, a Scottish bat conservationist developed a rabies-like disease and subsequently died. This was caused by infection with European bat lyssavirus 2 (EBLV-2), a virus closely related to Rabies virus (RABV). The source of this infection and the means of transmission have not yet been confirmed. In this study, the...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.46370-0
更新日期:2006-06-01 00:00:00
abstract::The cell-wall characteristics of Gardnerella vaginalis grown in conventional and biofilm systems were studied by electron microscopy. The gram-positive nature of the cell wall was confirmed. Novel cell-wall particles which appeared to be associated with cell division were also identified, particularly in organisms of ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-48-2-211
更新日期:1999-02-01 00:00:00
abstract::Fusarium spp. and other non-dermatophyte fungi are repeatedly isolated from abnormal nails. To investigate whether these fungi are the aetiological agents of infection or simply transient contaminants, a PCR/sequencing/RFLP assay was developed for direct and routine identification of the infecting fungi in onychomycos...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.46723-0
更新日期:2006-09-01 00:00:00
abstract::The enzymic activity of 29 Haemophilus ducreyi strains on 28 substrates is described. The results are compared with those of seven other authors. There is agreement only about the presence of alkaline phosphatase and arginine aminopeptidase and the lack of glycosidases. Possible reasons for the contradictions in the e...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-18-2-181
更新日期:1984-10-01 00:00:00
abstract::A rabbit was immunised with a heat-killed vaccine prepared from strain 1142 of Staphylococcus epidermidis. The HA titres of the antisera against a surface-polysaccharide antigen extracted from the homologous strain were highest in the 2nd-4th weeks after immunisation and subsequently declined. Only a minor amount of 2...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-11-4-371
更新日期:1978-11-01 00:00:00
abstract::Introduction. The emergence of novel strains of Vibrio cholerae O1 El Tor biotype has gained attention due to causing several epidemics around the world. Variant strains have evolved as a result of the acquisition of genes that confer extended virulence and pathogenicity.Aim. This study aimed to determine the presence...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.001131
更新日期:2020-03-01 00:00:00
abstract::Eikenella corrodens, a commensal of the human oral cavity, is generally associated with bite wounds and head and neck infections. Neonatal infections are rare. We report two cases of premature birth associated with maternofetal E. corrodens infection. ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.002568-0
更新日期:2009-02-01 00:00:00
abstract::Clostridium botulinum type A toxin is the most prevalent cause of naturally occurring outbreaks of human botulism in the world. The active dichain neurotoxin molecule is composed of a heavy chain (H-chain) of ~100 kDa with the carboxy-terminal end consisting of a receptor-binding (HC) domain, while the amino-terminal ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.053124-0
更新日期:2013-06-01 00:00:00
abstract::The abilities of six strains of Salmonella typhimurium to associate with rabbit ileal mucosa have been measured in vitro. Two were "virulent" strains (TML and W118 which are invasive and inducers of fluid secretion in rabbit ileal loops); four were "avirulent" (LT7, M206 and SL1027 which are invasive but induce neglig...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-29-4-283
更新日期:1989-08-01 00:00:00
abstract::Multi-drug-resistant tuberculosis (MDR-TB) is a major public-health problem, because treatment is complicated and patients remain infectious for months or years, despite receiving the best available therapy. To gain better understanding of MDR-TB, a retrospective study was initiated to determine the level of drug resi...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.45635-0
更新日期:2005-03-01 00:00:00
abstract::The encapsulated strain M of Staphylococcus aureus and the encapsulated strain 76 of S. simulans survived incubation with bovine neutrophils in media containing heated bovine serum 0.5% v/v or milk whey 10% v/v. With two unencapsulated strains of S. aureus, M12 and M60, less than 5% of the original inoculum survived. ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-20-3-317
更新日期:1985-12-01 00:00:00
abstract:PURPOSE:This study investigates the virulence and antimicrobial resistance in association with common clonal complexes (CCs) of enteroaggregative Escherichia coli (EAEC) isolated from Bangladesh. The aim was to determine whether specific CCs were more likely to be associated with putative virulence genes and/or antimic...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000594
更新日期:2017-10-01 00:00:00
abstract::The prevalence of Shiga toxin-producing Escherichia coli (STEC) and its characteristics were determined among hospitalized patients with diarrhoea and children with diarrhoea in an urban slum community of Dhaka city using sensitive culture and PCR methods. Stool samples were collected from 410 patients with diarrhoea ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.46916-0
更新日期:2007-03-01 00:00:00
abstract::Bifidobacteria are dominant in the gut of full-term infants, although colonisation by them is often delayed in preterm neonates. Bifidobacteria are recognised to have beneficial effects on digestive disorders and they might prevent neonatal necrotising enterocolitis (NEC), a gastrointestinal disease that predominantly...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-48-1-89
更新日期:1999-01-01 00:00:00