Fast and reliable PCR/sequencing/RFLP assay for identification of fungi in onychomycoses.

Abstract:

:Fusarium spp. and other non-dermatophyte fungi are repeatedly isolated from abnormal nails. To investigate whether these fungi are the aetiological agents of infection or simply transient contaminants, a PCR/sequencing/RFLP assay was developed for direct and routine identification of the infecting fungi in onychomycosis. Fungal DNA was readily extracted using a commercial kit after dissolving nail fragments in a Na2S solution. Amplification of part of the 28S rDNA by PCR was performed with universal primers and the fungal species were identified by sequencing. The PCR/sequencing results were comparable with microbiological identification from the same nail sample. In addition to dermatophytes, Fusarium spp. and other less frequently isolated non-dermatophyte fungi were identified as single fungal agents in onychomycosis. Moreover, mixed infections were clearly demonstrated in 10% of cases by RFLP analysis of PCR products. Identification of infectious agents could be obtained in 2 days, whilst results from fungal cultures take 1-3 weeks. Rapid and reliable molecular identification of the infectious fungus expedites the choice of appropriate antifungal therapy, thereby improving the cure rate of onychomycosis.

journal_name

J Med Microbiol

authors

Monod M,Bontems O,Zaugg C,Léchenne B,Fratti M,Panizzon R

doi

10.1099/jmm.0.46723-0

subject

Has Abstract

pub_date

2006-09-01 00:00:00

pages

1211-1216

issue

Pt 9

eissn

0022-2615

issn

1473-5644

journal_volume

55

pub_type

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