PCR evaluation of false-positive signals from two automated blood-culture systems.

abstract：:Trichoderma longibrachiatum is an emerging pathogen in immunocompromised patients. We report a case of Trichoderma post-operative mediastinitis and peritonitis in a child with complex congenital cardiac disease and functional asplenia. The patient was treated unsuccessfully, initially with caspofungin alone followed b...

journal_title：Journal of medical microbiology

authors： Santillan Salas CF,Joshi AY,Dhiman N,Banerjee R,Huskins WC,Wengenack NL,Henry NK

更新日期：2011-12-01 00:00:00

abstract：:The potential of exocellular carbohydrate antigens of Staphylococcus epidermidis as markers of infection in bone was investigated by immunoblotting and enzyme-linked immunosorbent assay (ELISA). Exocellular antigens were prepared by gel filtration chromatography of concentrated brain heart infusion culture supernates....

journal_title：Journal of medical microbiology

authors： Lambert PA,Van Maurik A,Parvatham S,Akhtar Z,Fraise AP,Krikler SJ

更新日期：1996-05-01 00:00:00

abstract：:This study investigated the molecular mechanisms of carbapenem resistance in clinical isolates of Klebsiella pneumoniae from Korea and the clinical outcomes of resistant K. pneumoniae infection. Sixteen K. pneumoniae isolates showing resistance to carbapenems collected from a tertiary-care hospital were examined for t...

journal_title：Journal of medical microbiology

authors： Shin SY,Bae IK,Kim J,Jeong SH,Yong D,Kim JM,Lee K

更新日期：2012-02-01 00:00:00

abstract：:A periplasmic flagellin gene, flaB, of Leptospira interrogans serovar pomona (strain Pomona) was expressed in Escherichia coli for the production and antigenic characterisation of the protein. The flaB structural gene, which was previously cloned into pUC118, was derived by PCR from the recombinant plasmid and used to...

journal_title：Journal of medical microbiology

authors： Lin M,Bughio N,Surujballi O

更新日期：1999-11-01 00:00:00

abstract：:Mice were vaccinated with lipopolysaccharide (LPS) from Leptospira interrogans serovar pomona or hardjo, or with the polysaccharide (PS) fraction of the LPS, or with an immunoconjugate of PS and diphtheria toxoid (DT). Maximum agglutinin titres were found 6-10 weeks after vaccination with LPS or PS-DT conjugate; the l...

journal_title：Journal of medical microbiology

authors： Midwinter A,Faine S,Adler B

更新日期：1990-11-01 00:00:00

abstract：:An affinity procedure with purified, biotinylated human transferrin and streptavidin-agarose was used to identify the transferrin-binding proteins in strains of Haemophilus influenzae. Proteins of 58 and 98 Kda were isolated from total membranes prepared from iron-deficient but not iron-sufficient H. influenzae KC548 ...

journal_title：Journal of medical microbiology

authors： Schryvers AB

更新日期：1989-06-01 00:00:00

abstract：:Immunodiffusion analysis of Mycobacterium lepraemurium indicated the presence of at lease six antigens. Comparative analysis of the M. lepraemurium antigen-antibody system with similar systems established for other mycobacterial species, showed that M. lepraemurium shared up to two antigens with other species. Althoug...

journal_title：Journal of medical microbiology

authors： Navalkar RG,Dalvi RR,Patel PJ

更新日期：1975-02-01 00:00:00

abstract：:Human granulocytic anaplasmosis (HGA) and Lyme borreliosis (LB) are tick-borne infectious diseases caused by Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato species, respectively. In this study, p44/msp2 paralogues specific to A. phagocytophilum and 5S-23S rRNA gene-intergenic spacers specific to B. burg...

journal_title：Journal of medical microbiology

authors： Masuzawa T,Kharitonenkov IG,Okamoto Y,Fukui T,Ohashi N

更新日期：2008-08-01 00:00:00

abstract：:This study used a previously described multiplex PCR-based reverse line blot (mPCR/RLB) assay to assess the prevalence and distribution of 14 urogenital pathogens or putative pathogens, namely Neisseria gonorrhoeae, Chlamydia trachomatis, Mycoplasma genitalium, Mycoplasma hominis, Trichomonas vaginalis, Gardnerella va...

journal_title：Journal of medical microbiology

authors： McKechnie ML,Hillman RJ,Jones R,Lowe PC,Couldwell DL,Davies SC,King F,Kong F,Gilbert GL

更新日期：2011-07-01 00:00:00

abstract：:Detection of latent Mycobacterium tuberculosis is a challenge in the diagnosis of asymptomatic, subclinical tuberculosis. We report the development of an immunofluorescence technique to visualize and enumerate M. tuberculosis in latently infected rabbit lungs where no acid-fast-stained organisms were seen and no culti...

journal_title：Journal of medical microbiology

authors： Subbian S,Eugenin E,Kaplan G

更新日期：2014-11-01 00:00:00

abstract：:Gardnerella vaginalis is an important component of the human vaginal microflora. It is proposed to play a key role in the pathogenesis of bacterial vaginosis (BV), the most common vaginal condition. Here we describe the development, validation and comparative analysis of a novel molecular approach capable of G. vagina...

journal_title：Journal of medical microbiology

authors： Balashov SV,Mordechai E,Adelson ME,Gygax SE

更新日期：2014-02-01 00:00:00

abstract：:Extracts of 19 samples of pus which showed red fluorescence with ultraviolet light were screened for the presence of porphyrins by absorption spectrophotometry. All those which showed spectra typical of metal-free porphyrins were analysed by high performance liquid chromatography to identify the porphyrins present. Th...

journal_title：Journal of medical microbiology

authors： Brazier JS

更新日期：1990-09-01 00:00:00

abstract：:Chemotaxis of polymorphonuclear leukocytes (PMNL) in response to cell components of Bacteroides fragilis alone or in combination with Escherichia coli was evaluated. E. coli produced much more powerful chemotactic factors than B. fragilis. The culture filtrate (CF), outer membrane (OM) preparation, and lipopolysacchar...

journal_title：Journal of medical microbiology

authors： Namavar F,Verweij-Van Vught AM,Bal M,MacLaren DM

更新日期：1987-09-01 00:00:00

abstract：:The characterization of integrons and their promoters in 156 antibiotic-resistant clinical isolates of the important zoonotic pathogens Salmonella enterica subsp. enterica serovars Typhimurium (S. Typhimurium) and Enteritidis (S. Enteritidis) from Norwegian hospitals were performed. Integrons were found in 64 of 66 S....

journal_title：Journal of medical microbiology

authors： Lindstedt BA,Heir E,Nygård I,Kapperud G

更新日期：2003-02-01 00:00:00

abstract：PURPOSE:This study aimed to detect and identify staphylococcal enterotoxin (SE) genes in methicillin-resistant Staphylococcus pseudintermedius (MRSP) strains from different sources, and to investigate the relationship between their sequence types (STs) and SE gene patterns. METHODOLOGY:The profiles of 17 SE genes in 9...

journal_title：Journal of medical microbiology

authors： Phumthanakorn N,Fungwithaya P,Chanchaithong P,Prapasarakul N

更新日期：2018-06-01 00:00:00

abstract：:Escherichia coli isolates (n=2629) were collected between 1996 and 2000 from 2100 Thai children less than 12 years of age with acute diarrhoea. Enterotoxigenic (ETEC), enteroinvasive (EIEC), Shiga-toxin-producing (STEC), enteropathogenic (EPEC) and enteroaggregative (EAEC) E. coli were identified by their virulence ma...

journal_title：Journal of medical microbiology

authors： Ratchtrachenchai OA,Subpasu S,Hayashi H,Ba-Thein W

更新日期：2004-03-01 00:00:00

abstract：:In this study, we investigated the in vitro antifungal effects of itraconazole/voriconazole (ITR/VRC) alone and in combination with tetrandrine (TET) against 23 clinical isolates of A. fumigatus using a chequerboard microdilution method. The dynamic antifungal effects of TET with ITR/VRC against A. fumigatus were asse...

journal_title：Journal of medical microbiology

authors： Li SX,Song YJ,Zhang LL,Shi JP,Ma ZL,Guo H,Dong HY,Li YM,Zhang H

更新日期：2015-09-01 00:00:00

abstract：:Recent studies have shown that Providencia alcalifaciens is a diarrhoeal pathogen. It may cause diarrhoea by an invasive mechanism, as it invades cultured mammalian cells in vitro and intestinal epithelial cells of experimentally inoculated rabbits in vivo. To locate the gene(s) involved in invasion, TnphoA mutants of...

journal_title：Journal of medical microbiology

authors： Rahman M,Monira S,Nahar S,Ansaruzzaman M,Alam K,Alam M,Albert MJ

更新日期：2002-08-01 00:00:00

abstract：:Pseudomonas aeruginosa is an important opportunistic bacterial pathogen. Despite its metabolic and virulence versatility, it has not been shown to infect articular joints, which are areas that are rarely infected with bacteria in general. We hypothesized that articular joints possess antimicrobial activity that limits...

journal_title：Journal of medical microbiology

authors： Mahmood F,Hakimiyan A,Jayaraman V,Wood S,Sivaramakrishnan G,Rehman T,Reuhs BL,Chubinskaya S,Shafikhani SH

更新日期：2013-04-01 00:00:00

abstract：:Enterovirus 71 (EV71) and coxsackievirus A16 (CVA16) are members of the species Human enterovirus A, and are both major and independent aetiological agents of hand-foot-and-mouth disease. The human enterovirus (HEV) 5' untranslated region (UTR) is fundamentally important for efficient virus replication and for virulen...

journal_title：Journal of medical microbiology

authors： Zhou F,Kong F,Wang B,McPhie K,Gilbert GL,Dwyer DE

更新日期：2011-03-01 00:00:00

abstract：:In recent years, several plasmids harbouring genes encoding phosphoethanolamine transferases conferring colistin resistance have been described in multiple Enterobacteriaceae species. Avian Pathogenic E. coli (APEC) causes colibacillosis and is responsible for a considerable proportion of the disease burden in commerc...

journal_title：Journal of medical microbiology

authors： Hornsey M,Betts JW,Mehat JW,Wareham DW,van Vliet AHM,Woodward MJ,La Ragione RM

更新日期：2019-01-01 00:00:00

abstract：:Screening for Chlamydia trachomatis infections can be performed on urine samples and genital swabs using molecular techniques. A novel approach was developed that combined an automated extraction procedure, an automated liquid-handling system and real-time PCR to detect C. trachomatis from urine or swabs. This novel r...

journal_title：Journal of medical microbiology

authors： Jaton K,Bille J,Greub G

更新日期：2006-12-01 00:00:00

abstract：:A multiplex PCR was developed to detect 15 serotypes of Streptococcus suis. This multiplex PCR was separated into two reaction sets. The first set identified nine serotypes (serotypes 1/2, 1, 2, 3, 4, 7, 9, 14 and 16) and the second set identified six serotypes (serotypes 5, 8, 10, 19, 23 and 25). This assay correctly...

journal_title：Journal of medical microbiology

authors： Kerdsin A,Dejsirilert S,Akeda Y,Sekizaki T,Hamada S,Gottschalk M,Oishi K

更新日期：2012-12-01 00:00:00

abstract：:A novel aminopeptidase was purified by high performance liquid chromatography from a cytosoluble 100,000 g extract of Candida albicans on the basis of its ability to cleave L-arginine 7-amino-4-methylcoumarin. The purification factor was 36 and the yield was 20%. The native enzyme had a mol. wt of 52 kDa as demonstrat...

journal_title：Journal of medical microbiology

authors： el Moudni B,Rodier MH,Barrault C,Ghazali M,Jacquemin JL

更新日期：1995-10-01 00:00:00

abstract：:Electronmicrographs of sections show that the L-phases of Bacillus licheniformis var. endoparasiticus (Benedek) simulate the various types of micro-organism described by previous workers as associated with ostensibly noninfective conditions, notably cancer and arthritis; e.g., mycoplasmas, mycobacteria, corynebacteria...

journal_title：Journal of medical microbiology

authors： Bisset KA,Tallack J,Bartlett R

更新日期：1979-11-01 00:00:00

abstract：:The acquisition of Klebsiella aerogenes by neonates born in hospital has been studied by sero- and klebecin typing. This organism was more commonly carried in the bowel of breast-fed babies than of bottle-fed babies. Only very rarely did babies acquire strains of K. aerogenes from their mothers. K. aerogenes was widel...

journal_title：Journal of medical microbiology

authors： Shinebaum R,Cooke EM,Brayson JC

更新日期：1979-05-01 00:00:00

abstract：:The aim of this study was to assess the primary and combined resistances of Helicobacter pylori isolates obtained from paediatric patients in 2000-2001 to seven antimicrobial agents. Resistance rates of pre-treatment isolates from 115 children were investigated by the limited agar dilution method alone and by the E-te...

journal_title：Journal of medical microbiology

authors： Boyanova L,Koumanova R,Gergova G,Popova M,Mitov I,Kovacheva Y,Derejian S,Katsarov N,Nikolov R,Krastev Z

更新日期：2002-09-01 00:00:00

abstract：:Introduction. Melioidosis, caused by Burkholderia pseudomallei, in endemic areas, poses a challenge for treating the diseased populations without accurate diagnosis, and the disease-specific biomarkers linked with the infection have yet to be reported. Due to the invasive nature of the causative agent, Burkholderia ps...

journal_title：Journal of medical microbiology

authors： Saikh KU,Ranji CM,Ulrich RG,Corea E,De Silva AD,Natesan M

更新日期：2020-10-01 00:00:00

abstract：:The toxic effects of the haemorrhagic toxin of Clostridium sporogenes were studied in mice, rats, guinea-pigs and rabbits, and in various cultured cells. In rabbits, but not in the other animals, intradermal injection with crude toxin and its injection into a ligated intestinal loop caused haemorrhage in both the skin...

journal_title：Journal of medical microbiology

authors： Hara-Kudo Y,Ogura A,Noguchi Y,Kumagai S

更新日期：1997-04-01 00:00:00

abstract：:Neisseria sicca is rarely associated with clinical infections and to the authors' knowledge this organism has not been reported as a causative agent of infected biloma (liver abscess). A case of a diabetic man with infected biloma due to N. sicca after repeated transcatheter arterial embolization for hepatocellular ca...

journal_title：Journal of medical microbiology

authors： Chung HC,Teng LJ,Hsueh PR

更新日期：2007-11-01 00:00:00