Expression in Escherichia coli of flaB, the gene coding for a periplasmic flagellin of Leptospira interrogans serovar pomona.

abstract：:A monospecific polyclonal antiserum, prepared against Bacteroides fragilis common polysaccharide antigen purified by polyacrylamide gel immunoblot detected B. fragilis, B. thetaiotaomicron, B. ovatus and Prevotella melaninogenica in pus samples from various anatomical sites by immunofluorescence microscopy of the pus....

journal_title：Journal of medical microbiology

authors： Patrick S,Stewart LD,Damani N,Wilson KG,Lutton DA,Larkin MJ,Poxton I,Brown R

更新日期：1995-08-01 00:00:00

abstract：:This study investigated the comparative performance of the Amplicor assay and an in-house semi-automated, multiplex real-time PCR for the diagnosis of genital chlamydial infection. Four different assays, the COBAS Amplicor CT test (Amplicor PCR), in-house real-time PCR (IHRT-PCR), in-house nested cryptic plasmid PCR a...

journal_title：Journal of medical microbiology

authors： Jalal H,Al-Suwaine A,Stephen H,Carne C,Sonnex C

更新日期：2007-03-01 00:00:00

abstract：:It is believed that most microbial infections are caused by pathogens organized in biofilms. Recently, it was shown that the dimorphic fungus Histoplasma capsulatum, estimated to be the most common cause of fungal respiratory diseases, is also able to form biofilm. Although the antifungal therapy commonly used is effe...

journal_title：Journal of medical microbiology

authors： Brilhante RSN,de Lima RAC,Marques FJF,Silva NF,Caetano ÉP,Castelo-Branco DSCM,Bandeira TJPG,Moreira JLB,Cordeiro RA,Monteiro AJ,Camargo ZP,Sidrim JJC,Rocha MFG

更新日期：2015-04-01 00:00:00

abstract：PURPOSE:Meticillin-resistant Staphylococcus aureus (MRSA) biofilm formation in humans is of serious clinical concern. Previous in vitro studies have been performed with biofilms grown only on inorganic substrates; therefore, we investigated the vancomycin (VCM) resistance of MRSA biofilms grown on skin tissue. METHODO...

journal_title：Journal of medical microbiology

authors： Jimi S,Miyazaki M,Takata T,Ohjimi H,Akita S,Hara S

更新日期：2017-04-01 00:00:00

abstract：:Forty carbapenem-resistant Klebsiella pneumoniae isolates were recovered from 28 patients from various sites in an intensive care unit in Zhejiang Provincial People's Hospital, China, over a 6 month period. PFGE analysis indicated that the 40 strains were all closely related. The MICs of carbapenems varied from 16 to ...

journal_title：Journal of medical microbiology

authors： Zhang R,Wang XD,Cai JC,Zhou HW,Lv HX,Hu QF,Chen GX

更新日期：2011-07-01 00:00:00

abstract：:Most past studies of cross-infection with Pseudomonas aeruginosa among cystic fibrosis (CF) patients in the UK suggest that it is a rare occurrence. However, two recent reports of highly transmissible strains in patients in regional centres in England (Liverpool and Manchester) have raised questions as to the extent o...

journal_title：Journal of medical microbiology

authors： Scott FW,Pitt TL

更新日期：2004-07-01 00:00:00

abstract：:Fifteen randomly selected nasopharyngeal (NP) swab specimens (culture-negative for influenza A virus) were spiked with influenza A virus and the nucleic acids were extracted and subjected to PCR amplification with Thermus aquaticus (Taq) and T. thermophilus (Tth) DNA polymerases. Products of the expected size, and giv...

journal_title：Journal of medical microbiology

authors： Poddar SK,Sawyer MH,Connor JD

更新日期：1998-12-01 00:00:00

abstract：:Of 28 isolates of Vibrio cholerae non-O1 (10 from diarrhoeal patients and 18 from environmental sources) examined for haemolytic activity and its correlation, if any, with enterotoxic activity, 24 showed haemolysis. The four non-haemolytic isolates showed haemolysis after consecutive passages through rabbit ileal loop...

journal_title：Journal of medical microbiology

authors： Singh DV,Shukla BN,Sanyal SC

更新日期：1996-07-01 00:00:00

abstract：:A novel bunyavirus associated with fever, thrombocytopenia and leukopenia syndrome (FTLS) was discovered in Henan Province, China. Here, we report the development of an assay for this novel bunyavirus based on real-time reverse transcription PCR (RT-PCR). The assay exhibited high sensitivity and specificity without cr...

journal_title：Journal of medical microbiology

authors： Huang X,Liu L,Du Y,Ma H,Mu Y,Pengzhi Wang,Ma H,Tang X,Wang H,Kang K,Zhang S,Wu W,Chen H,Liu G,Yang Y,Yongqiangj,Jiang,Xu B,Chen W

更新日期：2013-07-01 00:00:00

abstract：:The effect of pretreatment of denture acrylic with chlorhexidine gluconate on the subsequent adherence of Candida albicans GDH 2346 was measured in vitro. Adherence was significantly reduced by pretreatment with chlorhexidine; maximal inhibition was achieved by incubation at room temperature for 30 min in 2% chlorhexi...

journal_title：Journal of medical microbiology

authors： McCourtie J,MacFarlane TW,Samaranayake LP

更新日期：1985-08-01 00:00:00

abstract：:Eight monoclonal antibodies (MAbs) were produced from mice immunised with whole cells of heat-killed Pasteurella multocida type A which had been cultured under iron-restricted conditions. The MAbs were selected by an enzyme-linked immunosorbent assay (ELISA) in which the antigen consisted of whole bacteria of the immu...

journal_title：Journal of medical microbiology

authors： Wijewardana TG,Wilson CF,Gilmour NJ,Poxton IR

更新日期：1990-12-01 00:00:00

abstract：:The plasmid profiles, and their association with antimicrobial resistance, of 60 strains of Aeromonas hydrophila isolated from fish, shellfish and water were investigated. Only two strains were susceptible to all the antimicrobial agents tested; the highest incidences of resistance were to tetracycline (96.7%), prysta...

journal_title：Journal of medical microbiology

authors： Borrego JJ,Moriñigo MA,Martinez-Manzanares E,Bosca M,Castro D,Barja JL,Toranzo AE

更新日期：1991-11-01 00:00:00

abstract：:The effects of imipenem and meropenem on the transcriptional expression of resistance-related genes oprD, oprM and oprN in Pseudomonas aeruginosa were studied by quantitative real-time PCR. Four strains were examined: the type strain PT5 (PAO1), its derivatives M7 and PT149, and a clinical isolate, PaKT3. The derivati...

journal_title：Journal of medical microbiology

authors： Kolayli F,Karadenizli A,Savli H,Ergen K,Hatirnaz O,Balikci E,Budak F,Vahaboglu H

更新日期：2004-09-01 00:00:00

abstract：PURPOSE:Leptospira interrogans serogroup Icterohaemorrhagiae strains have been described as causing disease in both humans and animals and as being present worldwide. Icterohaemorrhagiae and Copenhageni serovars are known to cause severe disease in their hosts, and zoonotic outbreaks have been described. The genetic si...

journal_title：Journal of medical microbiology

authors： Jaeger LH,Pestana CP,Carvalho-Costa FA,Medeiros MA,Lilenbaum W

更新日期：2018-09-01 00:00:00

abstract：PURPOSE:Urinary tract infection (UTI) can be community-acquired (Com-UTI) or catheter-associated (CAUTI) and may be associated with biofilm-producing organisms. A comparative analysis of biofilm-producing property (BPP), antibiotic-sensitivity and multi-drug resistance (MDR) and their relation with the BPP of isolates ...

journal_title：Journal of medical microbiology

authors： Bardoloi V,Yogeesha Babu KV

更新日期：2017-07-01 00:00:00

abstract：:The effect of passive immunotherapy with antisera against heat-killed Pseudomonas aeruginosa and three of its exo-enzymes (elastase, alkaline protease and exotoxin A) in gut-derived P. aeruginosa sepsis was evaluated. Mice were given a suspension of P. aeruginosa strain D4 in their drinking water, together with ampici...

journal_title：Journal of medical microbiology

authors： Matsumoto T,Furuya N,Tateda K,Miyazaki S,Ohno A,Ishii Y,Hirakata Y,Yamaguchi K

更新日期：1999-08-01 00:00:00

abstract：:Ten recent clinical isolates of Salmonella serotype Typhimurium from man that were tested for their invasiveness in rabbit ileal explants in vitro, were compared with Typhimurium strain TML, a well-characterised invasive strain isolated from a case of human gastro-enteritis. Nine of the 10 strains showed invasiveness ...

journal_title：Journal of medical microbiology

authors： Bolton AJ,Martin GD,Osborne MP,Wallis TS,Stephen J

更新日期：1999-09-01 00:00:00

abstract：:In the present study, we examined the roles of natural killer T (NKT) cells in host defence against Legionella pneumophila in a mouse model. The survival rate of NKT cell-deficient Jalpha281 knock-out (KO) mice was significantly higher than that of wild-type mice. There was no bacterial overgrowth in the lungs, but Ja...

journal_title：Journal of medical microbiology

authors： Hayakawa K,Tateda K,Fuse ET,Matsumoto T,Akasaka Y,Ishii T,Nakayama T,Taniguchi M,Kaku M,Standiford TJ,Yamaguchi K

更新日期：2008-11-01 00:00:00

abstract：:Campylobacter fetus subsp. fetus infection can occur in female sheep, causing infertility or abortion. Despite extensive research on the effect of these bacteria on female fertility, little research has been done on the influence of C. fetus subsp. fetus on the male factor. Our objective was to examine the influence o...

journal_title：Journal of medical microbiology

authors： Zan Bar T,Yehuda R,Hacham T,Krupnik S,Bartoov B

更新日期：2008-11-01 00:00:00

abstract：INTRODUCTION:Idiopathic pulmonary fibrosis (IPF) is a chronic and ultimately fatal lung disease. One of the risk factors involved in the acquisition of IPF is viral infections, especially respiratory viruses. In the present study, we investigated the detection of respiratory viruses and the possible relationship betwee...

journal_title：Journal of medical microbiology

authors： Keyvani H,Moghoofei M,Bokharaei-Salim F,Mostafaei S,Javad Mousavi SA,Monavari SH,Esghaei M

更新日期：2017-11-01 00:00:00

abstract：:The purpose of this study was to evaluate the anti-chlamydial activities in vitro of liposome-encapsulated doxycycline (Dox) and tetracycline (Tet) in comparison with free Dox and Tet. Dox and Tet encapsulated in cationic (CAL), anionic (ANL) and neutral (NTL) liposomes by sonication, were quantified by high-performan...

journal_title：Journal of medical microbiology

authors： Sangare L,Morisset R,Ravaoarinoro M

更新日期：1999-07-01 00:00:00

abstract：PURPOSE:The aim of this work was to evaluate and optimize the identification of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica (usually known as the classical Bordetella species) using Bruker Biotyper matrix-assisted laser desorption ionization/time-of-flight mass spectrometry (MALDI-TOF M...

journal_title：Journal of medical microbiology

authors： Zintgraff J,Irazu L,Lara CS,Rodriguez M,Santos M

更新日期：2018-12-01 00:00:00

abstract：:Data on the occurrence and epidemiology of Aspergillus spp. in laying hens farms are scant. With the aims of determining levels of airborne contamination in laying hen farms and evaluating the potential risk of infection for workers and animals, 57 air samples from 19 sheds (Group I), 69 from faeces (Group II), 19 fro...

journal_title：Journal of medical microbiology

authors： Cafarchia C,Camarda A,Iatta R,Danesi P,Favuzzi V,Di Paola G,Pugliese N,Caroli A,Montagna MT,Otranto D

更新日期：2014-03-01 00:00:00

abstract：PURPOSE:This study aimed to investigate the effect of smoking on the buccal microbiome and to analyse the descriptive ability of each of the seven hypervariable regions in their 16S rRNA genes. METHODOLOGY:Microbiome compositions of 40 buccal swab samples collected from smokers (n =20) and non-smokers (n =20) were det...

journal_title：Journal of medical microbiology

authors： Karabudak S,Ari O,Durmaz B,Dal T,Basyigit T,Kalcioglu MT,Durmaz R

更新日期：2019-08-01 00:00:00

abstract：:Saksenaea erythrospora is a species of the order Mucorales recently described and reported as a cause of human mucormycosis. We report a case of S. erythrospora in a man involved in a serious sailing accident causing deep skin and soft tissue contamination with soil and water. Direct microscopic examination of the cli...

journal_title：Journal of medical microbiology

authors： Relloso S,Romano V,Landaburu MF,Herrera F,Smayevsky J,Veciño C,Mujica MT

更新日期：2014-02-01 00:00:00

abstract：:Of 200 isolates of Vibrio mimicus screened, one from water (N-57) agglutinated with V. cholerae O139 polyclonal antiserum (absorbed with a rough strain of V. cholerae only) and not with O139 polyclonal diagnostic antiserum (absorbed with the rough strain and V. cholerae O22 and O155). The antigenic relationship betwee...

journal_title：Journal of medical microbiology

authors： Ansaruzzaman M,Shimada T,Bhuiyan NA,Nahar S,Alam K,Islam MS,Albert MJ

更新日期：1999-09-01 00:00:00

abstract：:The genetic characteristics of Vibrio parahaemolyticus strains isolated in 2004 and 2005 in Mozambique were assessed in this study to determine whether the pandemic clone of V. parahaemolyticus O3 : K6 and O4 : K68 serotypes has spread to Mozambique. Fifty-eight V. parahaemolyticus strains isolated from hospitalized d...

journal_title：Journal of medical microbiology

authors： Ansaruzzaman M,Chowdhury A,Bhuiyan NA,Sultana M,Safa A,Lucas M,von Seidlein L,Barreto A,Chaignat CL,Sack DA,Clemens JD,Nair GB,Choi SY,Jeon YS,Lee JH,Lee HR,Chun J,Kim DW

更新日期：2008-12-01 00:00:00

abstract：:We report a third case of Mycobacterium conceptionense infection, which was found in a 50-year-old female following face rejuvenation surgery with fat grafting. The pathogen was identified using 16S rRNA gene and rpoB gene sequences. The growing diversity of non-tuberculosis mycobacterial species causing human infecti...

journal_title：Journal of medical microbiology

authors： Yang HJ,Yim HW,Lee MY,Ko KS,Yoon HJ

更新日期：2011-03-01 00:00:00

abstract：:Bacteriophages in different stages of maturation were found in thin sections of a clinical isolate of Helicobacter (Campylobacter) pylori. Mature phage heads measured 70 x 60 nm and the tail at least 120 nm. Lysogeny was maintained during subculture on blood agar for more than 3 months after isolation from a gastric b...

journal_title：Journal of medical microbiology

authors： Schmid EN,von Recklinghausen G,Ansorg R

更新日期：1990-06-01 00:00:00

abstract：:Resistance to desiccation and to skin fatty acids was measured in three groups of methicillin-resistant Staphylococcus aureus (MRSA) strains and a group of control strains. Organisms from a large outbreak on a special care baby unit (SCBU), where MRSA had been isolated from staff hands but not from the environment, we...

journal_title：Journal of medical microbiology

authors： Farrington M,Brenwald N,Haines D,Walpole E

更新日期：1992-01-01 00:00:00