The classical Bordetella species and MALDI-TOF technology: a brief experience.

abstract：:To gain a better understanding of the pathological role of interferon-gamma (IFN-gamma) in specific granuloma formation, IFN-gamma gene-deficient mice (BALB/c and C57BL/6) were produced. The IFN-gamma gene in embryonic stem (ES) cells was disrupted by inserting the beta-galactosidase gene (lacZ) and the neomycin resis...

journal_title：Journal of medical microbiology

authors： Sugawara I,Yamada H,Kazumi Y,Doi N,Otomo K,Aoki T,Mizuno S,Udagawa T,Tagawa Y,Iwakura Y

更新日期：1998-10-01 00:00:00

abstract：:The aim of this study was to evaluate an enzyme-linked immunosorbent assay (ELISA) for the detection of anti-Helicobacter pylori specific IgG antibodies in specimens of oral fluid. Antral biopsy specimens, serum and oral fluid samples were collected from 81 patients attending for upper gastrointestinal endoscopy. The ...

journal_title：Journal of medical microbiology

authors： Marshall B,Howat AJ,Wright PA

更新日期：1999-11-01 00:00:00

abstract：:During a one-year period, 258 isolates of Campylobacter jejuni and C. coli were obtained from children with gastroenteritis or bacteraemia at the Red Cross Children's Hospital, Cape Town, South Africa. These isolates were biotyped by hippurate hydrolysis, H2S production and tolerance to 2,3,5-triphenyltetrazolium chlo...

journal_title：Journal of medical microbiology

authors： Lastovica AJ,Le Roux E,Congi RV,Penner JL

更新日期：1986-02-01 00:00:00

abstract：:Antibiotic-resistant bacteria have emerged from the widespread use of antibiotics worldwide and have prompted the search for new sources of antimicrobial substances. Pinus spp. contain several bioactive compounds consisting mainly of terpenes, terpenoids and some other aromatic and aliphatic constituents. These compou...

journal_title：Journal of medical microbiology

authors： Leandro LF,Cardoso MJO,Silva SDC,Souza MGM,Veneziani RCS,Ambrosio SR,Martins CHG

更新日期：2014-12-01 00:00:00

abstract：:The cystic fibrosis (CF) airways are colonized by polymicrobial communities with high bacterial load and are influenced by frequent antibiotic exposures. This community includes diverse streptococci, some of which have been directly or indirectly associated with pulmonary exacerbations. As many streptococci are natura...

journal_title：Journal of medical microbiology

authors： Thornton CS,Grinwis ME,Sibley CD,Parkins MD,Rabin HR,Surette MG

更新日期：2015-11-01 00:00:00

abstract：:Invasive infections caused by Brevundimonas vesicularis are very rare in humans. We experienced an unusual case of liver abscess due to B. vesicularis in an immunocompetent young male. The patient was successfully treated by liver abscess drainage and with antimicrobial therapy of ceftriaxone followed by ampicillin/su...

journal_title：Journal of medical microbiology

authors： Yoo SH,Kim MJ,Roh KH,Kim SH,Park DW,Sohn JW,Yoon YK

更新日期：2012-10-01 00:00:00

abstract：:Campylobacter jejuni is a frequent cause of infectious diarrhoea and is increasingly recognized as a trigger for late-onset complications. The poor standardization of commonly used serological tests might explain the conflicting results regarding the frequency of antecedent C. jejuni infections in defined patient grou...

journal_title：Journal of medical microbiology

authors： Schmidt-Ott R,Brass F,Scholz C,Werner C,Groß U

更新日期：2005-08-01 00:00:00

abstract：:Burkholderia pseudomallei is isolated frequently from the soil in regions where the disease melioidosis occurs. However, recent surveys in Thailand have shown that the frequency of isolation of the organism from soil samples is not directly related to the incidence of melioidosis in an area. To determine whether strai...

journal_title：Journal of medical microbiology

authors： Trakulsomboon S,Dance DA,Smith MD,White NJ,Pitt TL

更新日期：1997-07-01 00:00:00

abstract：:The potential of incorporating a real-time PCR for amplification and detection of 16S rRNA gene signatures directly from clinical samples was assessed as a tool for diagnostics. Universal PCR primers spanning short variable regions (~500 bp) were optimized for real-time PCR and tested in comparison with a longer fragm...

journal_title：Journal of medical microbiology

authors： Edwards KJ,Logan JMJ,Langham S,Swift C,Gharbia SE

更新日期：2012-05-01 00:00:00

abstract：:Antigen participating in cell-mediated immunity was isolated from a sonic extract of Mycobacterium tuberculosis H37Rv. The purified antigen had a mol. wt 150 000-200 000, based upon its Rf in 7.5% polyacrylamide gel in the presence of sodium dodecyl sulphate. In simple agarose gel electrophoresis it had an Rf of 0.85....

journal_title：Journal of medical microbiology

authors： Hattikudur S,Kamat RS

更新日期：1984-08-01 00:00:00

abstract：:Enteroaggregative Escherichia coli (EAEC), a highly heterogeneous pathotype of E. coli classified as typical and atypical, are an emerging cause of acute and persistent diarrhea. We aimed to investigate whether population living in rural geographic areas, impacts in the heterogeneity, dissemination and antimicrobial s...

journal_title：Journal of medical microbiology

authors： Guerrieri CG,Monfardini MV,Silva EA,Bueno de Freitas L,Schuenck RP,Spano LC

更新日期：2020-01-01 00:00:00

abstract：:Meticillin-resistant Staphylococcus aureus (MRSA) is a major nosocomial pathogen in Pakistan and is emerging in the community. This is one of the first reports of the prevalent genotypes of MRSA in both hospital and community settings in Pakistan. Isolates collected in 2006-2007 were characterized by PFGE, staphylococ...

journal_title：Journal of medical microbiology

authors： Zafar A,Stone M,Ibrahim S,Parveen Z,Hasan Z,Khan E,Hasan R,Wain J,Bamford K

更新日期：2011-01-01 00:00:00

abstract：:Of 2458 isolates of Haemophilus influenzae examined in a recent British survey, 42 were resistant to chloramphenicol. Two resistant isolates were of type b and 40 were non-capsulate. Spectrophotometric assay showed that all the resistant isolates produced chloramphenicol acetyltransferase (CAT). CAT activity did not i...

journal_title：Journal of medical microbiology

authors： Powell M,Livermore DM

更新日期：1988-10-01 00:00:00

abstract：:Enterotoxigenic Escherichia coli (ETEC) strain P2200 of porcine origin possessed eight possibly plasmid-determined characters (K88+ Raf+ Hly+ Col+ Smr Tcr Su(r) STa+) and six plasmid DNA bands of 4.2-93 kb. Analysis of the spontaneous loss of characters and the results of matings with other E. coli strains revealed th...

journal_title：Journal of medical microbiology

authors： Bertin A

更新日期：1992-08-01 00:00:00

abstract：:Following characterisation by phenotypic tests and amplified ribosomal DNA restriction analysis (ARDRA), 50 tetracycline-resistant (MIC > or = 16 mg/L) Acinetobacter strains from clinical (n = 35) and aquatic (n = 15) samples were analysed by PCR for tetracycline resistance (Tet) determinants of classes A-E. All the c...

journal_title：Journal of medical microbiology

authors： Guardabassi L,Dijkshoorn L,Collard JM,Olsen JE,Dalsgaard A

更新日期：2000-10-01 00:00:00

abstract：:Currently, several PCR assays based on 16S rRNA and virulence-associated genes are available for detection of Legionella pneumophila. So far, no genotyping method has been published that can discriminate between serogroups and monoclonal subgroups of the most common L. pneumophila serogroup 1. Our first approach was t...

journal_title：Journal of medical microbiology

authors： Thürmer A,Helbig JH,Jacobs E,Lück PC

更新日期：2009-05-01 00:00:00

abstract：:Pseudomonas aeruginosa is an important opportunistic bacterial pathogen. Despite its metabolic and virulence versatility, it has not been shown to infect articular joints, which are areas that are rarely infected with bacteria in general. We hypothesized that articular joints possess antimicrobial activity that limits...

journal_title：Journal of medical microbiology

authors： Mahmood F,Hakimiyan A,Jayaraman V,Wood S,Sivaramakrishnan G,Rehman T,Reuhs BL,Chubinskaya S,Shafikhani SH

更新日期：2013-04-01 00:00:00

abstract：:The present study aimed to evaluate the role of biofilm morphology, matrix content and surface hydrophobicity in the biofilm-forming capacity of Candida albicans and non-albicans Candida (NAC) spp. Biofilm formation was determined by microtitre plate assay and bright-field and scanning electron microscopy. The matrix ...

journal_title：Journal of medical microbiology

authors： Kumari A,Mankotia S,Chaubey B,Luthra M,Singh R

更新日期：2018-06-01 00:00:00

abstract：:Of 28 isolates of Vibrio cholerae non-O1 (10 from diarrhoeal patients and 18 from environmental sources) examined for haemolytic activity and its correlation, if any, with enterotoxic activity, 24 showed haemolysis. The four non-haemolytic isolates showed haemolysis after consecutive passages through rabbit ileal loop...

journal_title：Journal of medical microbiology

authors： Singh DV,Shukla BN,Sanyal SC

更新日期：1996-07-01 00:00:00

abstract：:The rapid identification of pathogenic yeasts is a crucial step in ensuring that effective antifungal treatment is started as early as possible. CandiSelect 4 (CS4; Bio-Rad) is a new chromogenic medium for the isolation of fungi, the direct identification of Candida albicans and the presumptive identification of the m...

journal_title：Journal of medical microbiology

authors： Sendid B,François N,Standaert A,Dehecq E,Zerimech F,Camus D,Poulain D

更新日期：2007-04-01 00:00:00

abstract：:Five strains of mice (C3H, CBA, BALB/c, TO and ICR) were inoculated intra-vaginally with Neisseria gonorrhoeae in an attempt to produce an animal model of gonorrhoea. Of a total of 68 mice inoculated, only three (4.4%) were culture-positive after 3 days. Histological examination of both the genital mucosa of inoculate...

journal_title：Journal of medical microbiology

authors： Johnson AP,Tuffrey M,Taylor-Robinson D

更新日期：1989-09-01 00:00:00

abstract：:The main components of oral malodour have been identified as volatile sulfur compounds (VSCs), including hydrogen sulfide (H(2)S) and methyl mercaptan (CH(3)SH). The lactoperoxidase (LPO) system (consisting of LPO, glucose oxidase, glucose and thiocyanate) was previously shown to exhibit antimicrobial activities again...

journal_title：Journal of medical microbiology

authors： Nakano M,Shin K,Wakabayashi H,Yamauchi K,Abe F,Hironaka S

更新日期：2015-10-01 00:00:00

abstract：:Rapid identification of isolates in positive blood cultures are of great importance to secure correct treatment of septicaemic patients. As antimicrobial resistance is increasing, rapid detection of resistance is crucial. Carbapenem resistance in Bacteroides fragilis associated with cfiA-encoded class B metallo-beta-l...

journal_title：Journal of medical microbiology

authors： Johansson Å,Nagy E,Sóki J

更新日期：2014-08-01 00:00:00

abstract：:The strains of Proteus species found in significant numbers and as pure cultures in urine from 217 individuals were isolated, identified to species level and typed for proticine production (P type) and proticine sensitivity (S type) to give their P/S type. Urinary-tract infections with Proteus, principally P. mirabili...

journal_title：Journal of medical microbiology

authors： Senior BW

更新日期：1979-02-01 00:00:00

abstract：:Trichoderma longibrachiatum is an emerging pathogen in immunocompromised patients. We report a case of Trichoderma post-operative mediastinitis and peritonitis in a child with complex congenital cardiac disease and functional asplenia. The patient was treated unsuccessfully, initially with caspofungin alone followed b...

journal_title：Journal of medical microbiology

authors： Santillan Salas CF,Joshi AY,Dhiman N,Banerjee R,Huskins WC,Wengenack NL,Henry NK

更新日期：2011-12-01 00:00:00

abstract：:Antibodies specific for rubella virion polypeptide, VPI, secreted by clones of hybridoma cells or produced in rabbits in response to specific antigenic stimulation, located determinants for haemagglutination inhibiting (HI) and neutralising (Nt) antibodies on this envelope component. ...

journal_title：Journal of medical microbiology

authors： Ho-Terry L,Cohen A,Tedder RS

更新日期：1984-02-01 00:00:00

abstract：:Eight monoclonal antibodies (MAbs) were produced from mice immunised with whole cells of heat-killed Pasteurella multocida type A which had been cultured under iron-restricted conditions. The MAbs were selected by an enzyme-linked immunosorbent assay (ELISA) in which the antigen consisted of whole bacteria of the immu...

journal_title：Journal of medical microbiology

authors： Wijewardana TG,Wilson CF,Gilmour NJ,Poxton IR

更新日期：1990-12-01 00:00:00

abstract：:A non-tuberculous mycobacterium was isolated, following a vertebral needle aspiration, from the blood of a patient with severe spondylodiscitis. The strain turned out to be different from any known mycobacterial species and was quite drug-susceptible in vitro. The patient improved markedly following treatment with mer...

journal_title：Journal of medical microbiology

authors： Tortoli E,Mantella A,Mariottini A,Mazzarelli G,Pecile P,Rogasi PG,Sterrantino G,Fantoni E,Leoncini F

更新日期：2006-01-01 00:00:00

abstract：:Fifty-seven strains of acinetobacter-like organisms were isolated over a period of 26 months from the ears of 55 patients with acute or chronic otitis media, or otitis externa, and one strain was isolated in a survey of 50 normal ears. After comparison with eight reference strains, 32 of the isolates were identified a...

journal_title：Journal of medical microbiology

authors： Dadswell JV

更新日期：1976-08-01 00:00:00

abstract：:India contributes heavily to the global burden of visceral leishmaniasis (VL, kala-azar) and human immunodeficiency virus (HIV)/AIDS. The prevalence of HIV seropositivity in VL patients at a tertiary care centre in northern India, as observed during a prospective study over a period of 2 years, is presented. Of the 10...

journal_title：Journal of medical microbiology

authors： Mathur P,Samantaray JC,Vajpayee M,Samanta P

更新日期：2006-07-01 00:00:00