Abstract：
:Currently, several PCR assays based on 16S rRNA and virulence-associated genes are available for detection of Legionella pneumophila. So far, no genotyping method has been published that can discriminate between serogroups and monoclonal subgroups of the most common L. pneumophila serogroup 1. Our first approach was to analyse LPS-associated genes of seven L. pneumophila serogroup 1 strains, and we developed two PCR-based methods specific for serogroup 1. Specific DNA fragments could be amplified from all the serogroup 1 strains (n=43) including the strains from the American Type Culture Collection. In contrast, none of the strains from serogroups 2-15 (n=41) contained these specific gene regions. In a second approach, primers specific for the lag-1 gene, encoding an O-acetyltransferase, which is responsible for the presence of the LPS epitope recognized by mAb 3/1, were designed and tested for their ability to differentiate between mAb 3/1-positive and -negative strains. All mAb 3/1-positive strains (n=30) contained the lag-1 gene, but in turn 4 of 13 tested mAb 3/1-negative strains were also positive in the PCR. Thus, the discrimination between mAb 3/1-positive and mAb 3/1-negative subgroups could not be achieved for all strains. In a third approach, two intergenic regions expected to be specific for monoclonal subgroup Knoxville and closely related subgroups Benidorm/Bellingham were identified and used for selective genotyping. These intergenic regions could not only be amplified in every tested strain belonging to the subgroups Knoxville, Benidorm and Bellingham, but also in some strains of other unrelated subgroups. The two PCR approaches with primers specific for serogroup 1 genes definitely represent a valuable tool in outbreak investigations and for risk assessment. They also might be used for culture-independent diagnosis of legionellosis caused by L. pneumophila serogroup 1.
journal_name
J Med Microbioljournal_title
Journal of medical microbiologyauthors
Thürmer A,Helbig JH,Jacobs E,Lück PCdoi
10.1099/jmm.0.008508-0subject
Has Abstractpub_date
2009-05-01 00:00:00pages
588-595issue
Pt 5eissn
0022-2615issn
1473-5644journal_volume
58pub_type
杂志文章abstract：:Recent molecular studies have led to the recognition of three distinct species within the Candida parapsilosis complex, namely Candida parapsilosis, Candida orthopsilosis and Candida metapsilosis. As currently available yeast identification systems fail to differentiate these species, there is a paucity of information...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.008235-0
更新日期：2009-06-01 00:00:00
abstract：:Sorbarod biofilms were investigated for their suitability in establishing continuous culture biofilms for the study of bacterial vaginosis. Two important organisms in the condition, Gardnerella vaginalis and Lactobacillus acidophilus, were studied. In contrast to growth in broth culture, both organisms were maintained...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-47-5-401
更新日期：1998-05-01 00:00:00
abstract：:In order to develop novel host/pathogen real-time PCR assays for routine diagnostic use, early gene expression patterns from both Epstein-Barr virus (EBV) and Raji cells were examined after inducing the lytic life cycle using 12-O-tetradecanoyl-13-phorbol ester and sodium butyrate. Real-time PCR identified several hig...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.000352
更新日期：2016-11-01 00:00:00
abstract：:A total of 2401 isolates of Haemophilus parainfluenzae was isolated from respiratory secretions of 36 healthy adults and 128 patients with chronic bronchitis over a period of 1 year. The isolates were allocated to eight biotypes, by their production of indole, urease and ornithine decarboxylase. Biotypes I and II cons...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-36-4-279
更新日期：1992-04-01 00:00:00
abstract：:Antibody (AMI) and cell-mediated (CMI) immunity to rubella virus (RV) were evaluated in healthy adolescent males (n = 11) and females (n = 28) undergoing routine reimmunisation with RA27/3 strain RV as a component of measles-mumps-rubella (MMR) vaccine. Blood samples were collected just before and at 2, 4 and 10 weeks...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-48-12-1075
更新日期：1999-12-01 00:00:00
abstract：:Fifty clinical strains of Chlamydia trachomatis were studied by denaturing gradient gel electrophoresis (DGGE) of bacterial DNA amplified by the polymerase chain reaction (PCR). The strains belonged to the three most commonly encountered serovars in developed countries--D, E and F. Six reference strains, including the...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-43-1-14
更新日期：1995-07-01 00:00:00
abstract：:The strains of Proteus species found in significant numbers and as pure cultures in urine from 217 individuals were isolated, identified to species level and typed for proticine production (P type) and proticine sensitivity (S type) to give their P/S type. Urinary-tract infections with Proteus, principally P. mirabili...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-12-1-1
更新日期：1979-02-01 00:00:00
abstract：:Burkholderia pseudomallei is the causative agent of human and animal melioidosis. The role of quorum sensing (QS) in the in vivo pathogenicity of B. pseudomallei via inhalational exposure of BALB/c mice and intraperitoneal challenge of Syrian hamsters has not been reported. This investigation demonstrates that B. pseu...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.45661-0
更新日期：2004-11-01 00:00:00
abstract：:The aim of the present study was to investigate the colonization status of Clostridium difficile in healthy individuals. In total, 139 healthy adults from two study groups were examined at intervals of 3 months. Among the 18 positive subjects, the number of subjects from whom C. difficile was isolated once, twice, thr...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.05376-0
更新日期：2004-02-01 00:00:00
abstract：:Introduction. Current testing practices for yersiniosis mean that its true incidence and epidemiology are not well understood. In mid-2016, the introduction of testing via a multiplex gastrointestinal PCR panel at Portsmouth hospital laboratory in Hampshire, UK, resulted in a marked increase in the number of Yersinia ...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.001125
更新日期：2020-03-01 00:00:00
abstract：:We report a case of septicaemia with prolonged, refractory hypotension related to Moraxella osloensis isolated in a non-immunocompromised paediatric patient. ...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.016378-0
更新日期：2011-01-01 00:00:00
abstract：:Three specimens from 111 asymptomatic male partners of infertile couples attending the Department of Urology in Amiens, France, were examined by the PCR COBAS AMPLICOR test (Roche Molecular Diagnostics) for the presence of Chlamydia trachomatis. The specimens analysed were: first void urine (FVU), urine obtained after...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.45641-0
更新日期：2004-10-01 00:00:00
abstract：:Members of the Streptococcus anginosus group (SAG) are frequently involved in pyogenic infections in humans. In the present study, the antimicrobial susceptibility of 141 clinical SAG isolates to six antimicrobial agents was analysed by agar dilution. All isolates were susceptible to penicillin, cefotaxime and vancomy...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.001560-0
更新日期：2009-02-01 00:00:00
abstract：:In this study, we investigated the in vitro antifungal effects of itraconazole/voriconazole (ITR/VRC) alone and in combination with tetrandrine (TET) against 23 clinical isolates of A. fumigatus using a chequerboard microdilution method. The dynamic antifungal effects of TET with ITR/VRC against A. fumigatus were asse...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.000120
更新日期：2015-09-01 00:00:00
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journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-47-4-335
更新日期：1998-04-01 00:00:00
abstract：:Leucocyte-egested material was harvested after the quantitative in-vitro phagocytosis of Neisseria meningitidis by rabbit or mouse polymorphonuclear leucocytes. The egested material was injected subcutaneously into rabbits and followed 24 h later with an intravenous injection of what would by itself have been a non-le...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-9-4-451
更新日期：1976-11-01 00:00:00
abstract：:Effects on growth and toxin A production of sub-MIC concentrations of six different antibiotics were investigated in three strains of Clostridium difficile: reference strain NCTC 11223, a fully sequenced strain (630) and a locally endemic isolate (strain 338a). The antibiotics chosen for investigation were the agents ...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.05387-0
更新日期：2003-12-01 00:00:00
abstract：:Propionibacterium acnes, a commensal of human skin, is also an opportunistic pathogen of common acne and certain infectious diseases. However, it is still not obvious which strain is pathogenic for a certain infectious disease, and investigations to characterize pathogenic strains using molecular typing methods such a...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.040949-0
更新日期：2012-05-01 00:00:00
abstract：:Leifsonia aquatica is an aquatic coryneform rod that is capable of forming biofilms in environmental water sources. It has rarely been associated with human infections and its pathogenicity and clinical significance are uncertain. We describe a case of catheter-related bloodstream infection in a haemodialysis patient....
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.037457-0
更新日期：2012-06-01 00:00:00
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journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.04958-0
更新日期：2003-02-01 00:00:00
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journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-12-2-201
更新日期：1979-05-01 00:00:00
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journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-42-3-191
更新日期：1995-03-01 00:00:00
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journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-47-7-569
更新日期：1998-07-01 00:00:00
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journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-9-3-275
更新日期：1976-08-01 00:00:00
abstract：:Data on the occurrence and epidemiology of Aspergillus spp. in laying hens farms are scant. With the aims of determining levels of airborne contamination in laying hen farms and evaluating the potential risk of infection for workers and animals, 57 air samples from 19 sheds (Group I), 69 from faeces (Group II), 19 fro...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.065946-0
更新日期：2014-03-01 00:00:00
abstract：:Yokenella regensburgei is an opportunistic human pathogen of the Enterobacteriaceae family rarely reported to cause human infections. Here, we present a case report of Y. regensburgei bacteraemia from India clinically resembling enteric fever in an apparently immunocompetent paediatric patient. ...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.058669-0
更新日期：2013-06-01 00:00:00
abstract：:A total of 456 non-repetitive Escherichia coli isolates from human clinical specimens (urinary, n=134; cervix, vagina and prostate, n=52; blood, pus and wounds, n=45), healthy animals (cattle, n=45; poultry, n=20) and diseased animals (cattle, n=53; swine, n=64; poultry, n=43) obtained in Lithuania during the period 2...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/jmm.0.015008-0
更新日期：2010-03-01 00:00:00
abstract：:Since its discovery at the end of the nineteenth century, Moraxella (Branhamella) catarrhalis has undergone several changes of nomenclature and periodic changes in its perceived status as either a commensal or a pathogen. Molecular analysis based on DNA hybridisation or 16S rDNA sequence comparisons has established it...
journal_title：Journal of medical microbiology
pub_type： 杂志文章,评审
doi：10.1099/00222615-46-5-360
更新日期：1997-05-01 00:00:00
abstract：:A new 1,8-naphthyridine quinolone antimicrobial, PD131628, was found to be as, or more active than ciprofloxacin or ofloxacin against Escherichia coli and considerably more active than the two 4-quinolone agents against staphylococci in terms of both MIC and OBC (the optimum bactericidal concentration, at which the ra...
journal_title：Journal of medical microbiology
pub_type： 杂志文章
doi：10.1099/00222615-36-5-353
更新日期：1992-05-01 00:00:00
abstract：:Rickettsia conorii infection is endemic in the Mediterranean basin, where it is known as Mediterranean spotted fever, also known as Boutonneuse fever and Marseilles fever. We report the case of a 66-year-old diabetic man who presented a severe form of the disease, complicated by acute renal failure, thrombocytopenia a...
journal_title：Journal of medical microbiology
pub_type： 杂志文章,评审
doi：10.1099/jmm.0.004465-0
更新日期：2009-04-01 00:00:00
