Instant screening and verification of carbapenemase activity in Bacteroides fragilis in positive blood culture, using matrix-assisted laser desorption ionization--time of flight mass spectrometry.

abstract：:Antibodies to Shiga toxin (Stx) were measured in the sera of 49 children with Shigella dysenteriae serotype 1 infection, of whom 17 had haemolytic uraemic syndrome (HUS) and 32 had no complications (uncomplicated shigellosis, UCS). Children with HUS had lower levels of total IgG and IgM and lower IgM titres to Stx tha...

journal_title：Journal of medical microbiology

authors： Azim T,Rashid A,Qadri F,Sarker MS,Hamadani J,Salam MA,Wahed MA,Albert MJ

更新日期：1999-01-01 00:00:00

abstract：:The effects of ethyleneglycoltetra-acetic acid (EGTA) and EGTA + magnesium (MgEGTA) on the viable counts of 10 strains of Escherichia coli O6 have been studied in normal human serum (NHS), heat-inactivated serum (HIS) and in culture media with and without the addition of a beta-lactam antibiotic. The addition of EGTA ...

journal_title：Journal of medical microbiology

authors： Roberts AP,Phillips R

更新日期：1981-05-01 00:00:00

abstract：:A serial study of rotavirus infection in gnotobiotic piglets is described. They were infected when 7 days old and the course of infection was followed for 21 days, by immunofluorescence and histological examinations of the intestinal epithelium and by titration of the virus content of the gut lumen. There appeared to ...

journal_title：Journal of medical microbiology

authors： Crouch CF,Woode GN

更新日期：1978-08-01 00:00:00

abstract：:In recent years, several plasmids harbouring genes encoding phosphoethanolamine transferases conferring colistin resistance have been described in multiple Enterobacteriaceae species. Avian Pathogenic E. coli (APEC) causes colibacillosis and is responsible for a considerable proportion of the disease burden in commerc...

journal_title：Journal of medical microbiology

authors： Hornsey M,Betts JW,Mehat JW,Wareham DW,van Vliet AHM,Woodward MJ,La Ragione RM

更新日期：2019-01-01 00:00:00

abstract：:Herpes simplex virus type-1 (HSV-1) induces Fc- and C3b(i)-receptors on infected cells. The role of these receptors in bacterial superinfection was studied by comparing the adherence of non-opsonised and opsonised bacteria to HSV-infected and non-infected HEp-2 cells. A flow cytometric adherence assay, based on the fl...

journal_title：Journal of medical microbiology

authors： De Graaf-Miltenburg LA,Van Vliet KE,Ten Hagen TL,Verhoef J,Van Strijp JA

更新日期：1994-01-01 00:00:00

abstract：:To analyse the characteristics of infections caused by Streptococcus dysgalactiae subsp. equisimilis, clinical isolates (n=145) were collected at 11 medical institutions between September 2003 and October 2005. These isolates belonged to Lancefield group A (n=5), group C (n=18) or group G (n=122). Among all isolates, ...

journal_title：Journal of medical microbiology

authors： Sunaoshi K,Murayama SY,Adachi K,Yagoshi M,Okuzumi K,Chiba N,Morozumi M,Ubukata K

更新日期：2010-01-01 00:00:00

abstract：:Purpose. In this study, we differentiated between tuberculosis (TB) and infections caused by nontuberculous mycobacteria (NTM) or Nocardia in a tertiary general hospital in China. Differences in clinical manifestations and factors between respiratory infections associated with these organisms were also investigated.Me...

journal_title：Journal of medical microbiology

authors： Dong G,Chu P,Guo J,Xie Y,Lu J

更新日期：2020-09-01 00:00:00

abstract：:Human granulocytic anaplasmosis (HGA) and Lyme borreliosis (LB) are tick-borne infectious diseases caused by Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato species, respectively. In this study, p44/msp2 paralogues specific to A. phagocytophilum and 5S-23S rRNA gene-intergenic spacers specific to B. burg...

journal_title：Journal of medical microbiology

authors： Masuzawa T,Kharitonenkov IG,Okamoto Y,Fukui T,Ohashi N

更新日期：2008-08-01 00:00:00

abstract：:The aim of this study was to establish bacterial profiles in gastric biopsy specimens from patients with Helicobacter pylori-associated gastritis by means of temporal temperature gradient gel electrophoresis (TTGE) of PCR-amplified 16S rDNA fragments. Specimens from eight patients with asymptomatic gastritis and five ...

journal_title：Journal of medical microbiology

authors： Monstein HJ,Tiveljung A,Kraft CH,Borch K,Jonasson J

更新日期：2000-09-01 00:00:00

abstract：:Carbapenems are first-line agents for the treatment of serious nosocomial infections caused by multidrug-resistant Enterobacteriaceae. However, resistance to carbapenems has increased dramatically among Enterobacteriaceae in our hospital. In this study, we report clonal dissemination caused by carbapenem-resistant Ent...

journal_title：Journal of medical microbiology

authors： Qin X,Yang Y,Hu F,Zhu D

更新日期：2014-02-01 00:00:00

abstract：PURPOSE:In an increasing number of cases the last therapeutic option for treatment of carbapenem-resistant Enterobacteriaceae is colistin. As the detection of colistin resistance is problematic and time-consuming, it is desirable to find a rapid and reliable test. The rapid polymyxin NP test developed by Nordmann et al...

journal_title：Journal of medical microbiology

authors： Belda-Orlowski A,Pfennigwerth N,Gatermann SG,Korte-Berwanger M

更新日期：2019-08-01 00:00:00

abstract：:Active efflux has an important role in the antimicrobial resistance of Campylobacter jejuni and Campylobacter coli. The effects of two putative efflux pump inhibitors (EPIs), phenylalanine-arginine β-naphthylamide and 1-(1-naphthylmethyl)-piperazine, and the effects of inactivation of the cmeB,cmeF and cmeR genes on r...

journal_title：Journal of medical microbiology

authors： Mavri A,Smole Možina S

更新日期：2012-06-01 00:00:00

abstract：:A commercial method (RAPIDEC ur; bioMérieux UK Ltd, Basingstoke) and an in-house scheme based on conventional tests were compared for the identification of 179 commonly encountered gram-negative urinary pathogens. The methods identified correctly to genus level 97% and 84% of organisms, respectively. RAPIDEC ur was su...

journal_title：Journal of medical microbiology

authors： Batchelor BI

更新日期：1995-07-01 00:00:00

abstract：:The ability of various haem- and non-haem-iron-containing compounds to support the growth of iron-limited cultures of Haemophilus ducreyi was assessed in a plate bioassay. Only haemin or the haem-containing proteins, bovine haemoglobin, human haemoglobin and bovine catalase, but not equine cytochrome C111, were capabl...

journal_title：Journal of medical microbiology

authors： Lee BC

更新日期：1991-06-01 00:00:00

abstract：:Zygomycosis, a relatively uncommon infection, usually occurs among immunocompromised individuals. It has been reported only rarely in trauma patients. A fatal case is reported of pulmonary and rapidly progressive cutaneous zygomycosis in a young, otherwise healthy farmer, with multiple bone fractures, wounds and soft ...

journal_title：Journal of medical microbiology

authors： Kontogiorgi M,Floros I,Koroneos A,Vamvouka C,Paniara O,Roussos C,Routsi C

更新日期：2007-09-01 00:00:00

abstract：:Iliopsoas abscess is usually secondary to the spread of infection from a contiguous focus. Primary disease is uncommon, except in children where Staphylococcus aureus is the main pathogen. We report a 60-year-old woman who developed a primary iliopsoas abscess as a result of haematogenous spread of Capnocytophaga sput...

journal_title：Journal of medical microbiology

authors： Chan JFW,Wong SSY,Leung SSM,Li IWS,To KKW,Cheng VCC,Yuen KY

更新日期：2010-11-01 00:00:00

abstract：:Chemotaxis of polymorphonuclear leukocytes (PMNL) in response to cell components of Bacteroides fragilis alone or in combination with Escherichia coli was evaluated. E. coli produced much more powerful chemotactic factors than B. fragilis. The culture filtrate (CF), outer membrane (OM) preparation, and lipopolysacchar...

journal_title：Journal of medical microbiology

authors： Namavar F,Verweij-Van Vught AM,Bal M,MacLaren DM

更新日期：1987-09-01 00:00:00

abstract：:The effects of imipenem and meropenem on the transcriptional expression of resistance-related genes oprD, oprM and oprN in Pseudomonas aeruginosa were studied by quantitative real-time PCR. Four strains were examined: the type strain PT5 (PAO1), its derivatives M7 and PT149, and a clinical isolate, PaKT3. The derivati...

journal_title：Journal of medical microbiology

authors： Kolayli F,Karadenizli A,Savli H,Ergen K,Hatirnaz O,Balikci E,Budak F,Vahaboglu H

更新日期：2004-09-01 00:00:00

abstract：:The opsonic requirements of Helicobacter pylori were investigated in a series of experiments with human polymorphonuclear leucocytes (PMNL). Pre-incubation of H. pylori with pooled normal human serum (NHS) in concentrations of 5-20% significantly increased the uptake of radiolabelled bacteria by PMNL. Treatment of the...

journal_title：Journal of medical microbiology

authors： McKinlay AW,Young A,Russell RI,Gemmell CG

更新日期：1993-03-01 00:00:00

abstract：:The characterization and tracking of pathogenic micro-organisms in the clinical laboratory and public health environment demand schemes that are easy to standardize and use, are automated and high-throughput, and provide portable data. A combined genomarkers approach for Salmonella enterica based on comparative sequen...

journal_title：Journal of medical microbiology

authors： Bishop C,Honisch C,Goldman T,Mosko M,Keng S,Arnold C,Gharbia S

更新日期：2012-11-01 00:00:00

abstract：:Introduction. Current testing practices for yersiniosis mean that its true incidence and epidemiology are not well understood. In mid-2016, the introduction of testing via a multiplex gastrointestinal PCR panel at Portsmouth hospital laboratory in Hampshire, UK, resulted in a marked increase in the number of Yersinia ...

journal_title：Journal of medical microbiology

authors： Clarke M,Dabke G,Strakova L,Jenkins C,Saavedra-Campos M,McManus O,Paranthaman K

更新日期：2020-03-01 00:00:00

abstract：:The strains of Proteus species found in significant numbers and as pure cultures in urine from 217 individuals were isolated, identified to species level and typed for proticine production (P type) and proticine sensitivity (S type) to give their P/S type. Urinary-tract infections with Proteus, principally P. mirabili...

journal_title：Journal of medical microbiology

authors： Senior BW

更新日期：1979-02-01 00:00:00

abstract：PURPOSE:The aim of this work was to evaluate and optimize the identification of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica (usually known as the classical Bordetella species) using Bruker Biotyper matrix-assisted laser desorption ionization/time-of-flight mass spectrometry (MALDI-TOF M...

journal_title：Journal of medical microbiology

authors： Zintgraff J,Irazu L,Lara CS,Rodriguez M,Santos M

更新日期：2018-12-01 00:00:00

abstract：:The antifungal susceptibilities of 598 isolates of Candida spp. (bloodstream and other sterile sites) to liposomal amphotericin B (L-AmB) versus amphotericin B (AmB) were determined. MICs were calculated using the Clinical and Laboratory Standards Institute broth microdilution (M27-A3) method for L-AmB and the Etest m...

journal_title：Journal of medical microbiology

authors： Lovero G,Giglio O,Rutigliano S,Diella G,Caggiano G,Montagna MT

更新日期：2017-03-01 00:00:00

abstract：:Purpose. Antimicrobial susceptibility is slow to determine, taking several days to fully impact treatment. This proof-of-concept study assessed the feasibility of using machine-learning techniques for analysis of data produced by the flow cytometer-assisted antimicrobial susceptibility test (FAST) method we developed....

journal_title：Journal of medical microbiology

authors： Inglis TJJ,Paton TF,Kopczyk MK,Mulroney KT,Carson CF

更新日期：2020-05-01 00:00:00

abstract：:Iron is an important nutritional requirement for bacteria due to its conserved role in many essential metabolic processes. As a consequence of the lack of freely available iron in the mammalian host, bacteria upregulate a range of virulence factors during infection. Transcriptional analysis of Francisella tularensis s...

journal_title：Journal of medical microbiology

authors： Milne TS,Michell SL,Diaper H,Wikström P,Svensson K,Oyston PCF,Titball RW

更新日期：2007-10-01 00:00:00

abstract：:Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) is suitable for high-throughput and rapid microbial diagnosis at relatively low cost and can be considered an alternative to conventional biochemical and molecular identification systems in clinical microbiological laboratories...

journal_title：Journal of medical microbiology

authors： Jamal WY,Shahin M,Rotimi VO

更新日期：2013-04-01 00:00:00

abstract：:To gain insight into the value of lipopolysaccharide (LPS) core determinants for cross-protective immunisation the serological relationships between six complete (LPS) core types from Enterobacteriaceae were investigated. Hyperimmune sera were raised in mice by repeated immunisation with heat-killed strains of Salmone...

journal_title：Journal of medical microbiology

authors： Nnalue NA,Khan GN,Mustafa N

更新日期：1999-05-01 00:00:00

abstract：:We have characterised 45 isolates of methicillin-resistant Staphylococcus aureus (MRSA) from Glasgow Royal Infirmary by means of simple biotyping, immunoblotting of exported proteins and restriction enzyme fragmentation patterns (REFP) of plasmid DNA. The strains were subdivided into four groups (A-D) on the basis of ...

journal_title：Journal of medical microbiology

authors： Coia JE,Thomson-Carter F,Baird D,Platt DJ

更新日期：1990-02-01 00:00:00

abstract：:The attachment of Helicobacter pylori to the human gastric mucosa is a complex process involving several specific structures recognised by the cell surface receptors. Sialylated multivalent high mol. wt glycoproteins have been shown to inhibit H. pylori sialic acid-specific haemagglutination. This study explored wheth...

journal_title：Journal of medical microbiology

authors： Wang X,Hirmo S,Willén R,Wadström T

更新日期：2001-05-01 00:00:00