Abstract:
:The characterization and tracking of pathogenic micro-organisms in the clinical laboratory and public health environment demand schemes that are easy to standardize and use, are automated and high-throughput, and provide portable data. A combined genomarkers approach for Salmonella enterica based on comparative sequence analysis by mass spectrometry has been developed. The scheme targets genes encoding synthesis and assembly of antigens, metabolic pathway enzymes, virulence factors and fluoroquinolone resistance, covering the essential sequences that distinguish between and identify variation within serotypes. This study demonstrated how this single method could replace the combination of methods currently required to determine serotypes, subtypes, antibiotic resistance profiles and the genomic relatedness of Salmonella isolates. The results revealed genomic variation within seven serotypes previously unreported. This variation can be detected by using nucleotide sequence differences in the Salmonella flagellin gene fliC as markers that are not detected by traditional serotyping methods.
journal_name
J Med Microbioljournal_title
Journal of medical microbiologyauthors
Bishop C,Honisch C,Goldman T,Mosko M,Keng S,Arnold C,Gharbia Sdoi
10.1099/jmm.0.047431-0subject
Has Abstractpub_date
2012-11-01 00:00:00pages
1517-1524issue
Pt 11eissn
0022-2615issn
1473-5644journal_volume
61pub_type
杂志文章abstract::Antigen participating in cell-mediated immunity was isolated from a sonic extract of Mycobacterium tuberculosis H37Rv. The purified antigen had a mol. wt 150 000-200 000, based upon its Rf in 7.5% polyacrylamide gel in the presence of sodium dodecyl sulphate. In simple agarose gel electrophoresis it had an Rf of 0.85....
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-18-1-17
更新日期:1984-08-01 00:00:00
abstract:PURPOSE:We previously identified an association between CC22 meticillin-resistant Staphylococcus aureus (MRSA) bloodstream infection isolates with an elevated vancomycin MIC (V-MIC) in the susceptible range (1.5-2 mg l-1) and endocarditis. This study explores whether these isolates have a specific phenotype consistent ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000470
更新日期:2017-05-01 00:00:00
abstract::Early diagnosis of leptospirosis is important because severe leptospiral infection can run a fulminant course. The polymerase chain reaction (PCR) was evaluated for the detection of leptospires in clinical samples from patients with acute leptospiral infection. Blood and urine samples from 71 patients with leptospiros...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-43-2-110
更新日期:1995-08-01 00:00:00
abstract::Resistance to desiccation and to skin fatty acids was measured in three groups of methicillin-resistant Staphylococcus aureus (MRSA) strains and a group of control strains. Organisms from a large outbreak on a special care baby unit (SCBU), where MRSA had been isolated from staff hands but not from the environment, we...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-36-1-56
更新日期:1992-01-01 00:00:00
abstract::Introduction. Pseudomonas aeruginosa is an important Gram-negative pathogen that is intrinsically multidrug-resistant (MDR) and frequently associated with healthcare-associated outbreaks. With increasing resistance to antibiotics and with very few novel drugs under development, clinicians often use combinations to tre...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.001060
更新日期:2019-10-01 00:00:00
abstract:PURPOSE:The analytical performance of the cobas 6800 HIV-1, HBV and HCV assays was verified and evaluated to the COBAS Ampliprep/COBAS TaqMan assays. METHODOLOGY:The precision, limit of detection (LoD), limit of quantification (LoQ) and linearity were verified using pooled residual clinical samples. The analytical spe...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000838
更新日期:2018-12-01 00:00:00
abstract::A total of 36 Escherichia coli and 31 enterococci isolates were recovered from 42 common buzzard faecal samples. The E. coli isolates showed high levels of resistance to streptomycin and tetracycline. The following resistance genes were detected: bla(TEM) (20 of 22 ampicillin-resistant isolates), tet(A) and/or tet(B) ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.038364-0
更新日期:2012-06-01 00:00:00
abstract::The eubacterial population was studied in faecal samples of related and unrelated children. Temporal temperature gradient gel electrophoresis (TTGE) provided a snapshot of the bacterial population and allowed calculation of the degree of similarity in the predominant faecal microflora of identical twin pairs, fraterna...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.46189-0
更新日期:2005-12-01 00:00:00
abstract:PURPOSE:Urinary tract infection (UTI) can be community-acquired (Com-UTI) or catheter-associated (CAUTI) and may be associated with biofilm-producing organisms. A comparative analysis of biofilm-producing property (BPP), antibiotic-sensitivity and multi-drug resistance (MDR) and their relation with the BPP of isolates ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000525
更新日期:2017-07-01 00:00:00
abstract::The ability of rabbits to clear an intraperitoneal injection of Pseudomonas aeruginosa in the presence or absence of a surgically implanted peritoneal device was investigated. Sham-operated rabbits without an implant eliminated a P. aeruginosa challenge of 5 x 10(6) cfu/ml; lavage fluid and peritoneal tissues became c...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-36-6-406
更新日期:1992-06-01 00:00:00
abstract::Zygomycosis, a relatively uncommon infection, usually occurs among immunocompromised individuals. It has been reported only rarely in trauma patients. A fatal case is reported of pulmonary and rapidly progressive cutaneous zygomycosis in a young, otherwise healthy farmer, with multiple bone fractures, wounds and soft ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.46993-0
更新日期:2007-09-01 00:00:00
abstract::Nucleic acid amplification techniques (NAATs) have been demonstrated to make significant improvements in the diagnosis of tuberculosis (TB), particularly in the time to diagnosis and the diagnosis of smear-negative TB. The BD ProbeTec strand displacement amplification (SDA) system for the diagnosis of pulmonary and no...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.45780-0
更新日期:2004-12-01 00:00:00
abstract::Clostridium piliforme is an obligate intracellular bacterium that causes enterohepatic disease in many animal species. C. piliforme infections are commonly subclinical in laboratory rats and mice, and little is known about host regulation of disease or of the effects of C. piliforme infections on investigations that u...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/0022-1317-49-6-557
更新日期:2000-06-01 00:00:00
abstract::Resistance patterns and carbapenemase gene presence among Klebsiella pneumoniae isolates from the University General Hospital of Patras, Greece during a ten-year period were analysed under a surveillance programme for multi-drug-resistant bacteria. From 2005 to 2014, K. pneumoniae isolates from clinically significant ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000217
更新日期:2016-03-01 00:00:00
abstract::The purpose of this study was to evaluate the anti-chlamydial activities in vitro of liposome-encapsulated doxycycline (Dox) and tetracycline (Tet) in comparison with free Dox and Tet. Dox and Tet encapsulated in cationic (CAL), anionic (ANL) and neutral (NTL) liposomes by sonication, were quantified by high-performan...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-48-7-689
更新日期:1999-07-01 00:00:00
abstract::Although Neisseria gonorrhoeae can use haem as the sole exogenous iron source for growth in vitro, the mechanism of haem-iron uptake in the gonococcus is unknown. Two haemin-binding proteins (HmBPs) of 97 and 44 Kda were isolated by batch ligand affinity-chromatography from whole cells or total membranes of gonococci ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-36-2-121
更新日期:1992-02-01 00:00:00
abstract::The susceptibility of a strain of Giardia lamblia to benzimidazole carbamates, 5-nitroimidazoles, nitrofurans and other drugs was studied in vitro. Albendazole was the most active compound, with a 50% inhibitory concentration (IC50) of 0.01 mg/L and a minimal lethal concentration (MLC) of less than 0.04 mg/L; the IC50...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-37-3-221
更新日期:1992-09-01 00:00:00
abstract::Helicobacter trogontum is a micro-aerophilic urease-positive bacterium that has recently been isolated from the intestinal mucosa of rats. The purpose of this investigation was to study the ultrastructural details of this micro-organism in both pure culture and in the gastrointestinal tract of germ-free mice infected ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-47-6-513
更新日期:1998-06-01 00:00:00
abstract::Streptolysin O (SLO) is a potent cytolytic toxin produced by almost all strains of group A streptococci and is considered an important virulence factor for this organism. In this study we investigated the effect of allicin and aqueous garlic extracts on the haemolytic activity of SLO. All tested materials potentially ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.019539-0
更新日期:2010-09-01 00:00:00
abstract::Heated spores (80 degrees C, 10 min) of Clostridium butyricum strain CB 1002 isolated from a fatal case of necrotising enterocolitis in a human neonate were inoculated into ligated intestinal loops prepared in young conventional guinea pigs. Necropsy findings 18 h later included congestion, patchy haemorrhage of the i...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-19-3-351
更新日期:1985-06-01 00:00:00
abstract::Aggregatibacter actinomycetemcomitans is commonly part of the normal microflora of the human upper respiratory tract. It has been implicated in periodontal disease and various infections, particularly endocarditis. We report here what we believe to be the first case of recurrent infective endocarditis due to A. actino...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.024380-0
更新日期:2010-12-01 00:00:00
abstract:PURPOSE:Nodal may play an important role in the development of cancers. The present study was designed to determine the effects of Nodal induced by respiratory syncytial virus (RSV) infection on the occurrence and development of lung cancer and the underlying mechanisms. METHODOLOGY:After verification of RSV infection...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000581
更新日期:2017-10-01 00:00:00
abstract::Monoclonal antibodies (MAbs) N-59 and RU-1 were produced by immunisation of mice with streptokinase secreted by Streptococcus group A, type 12, strain A374 isolated from a patient with post-streptococcal glomerulonephritis (PSGN) and were characterised by Western blot analysis. MAb N-59 recognised antigenic determinan...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-35-1-60
更新日期:1991-07-01 00:00:00
abstract::The inhibitory effect of human immunodeficiency virus (HIV) proteinase inhibitors amprenavir and saquinavir and antifungal agents terbinafine, ketoconazole, amphotericin B and ciclopiroxolamine on aspartyl proteinases (Saps) secreted by Candida albicans was tested in an in vitro spectophotometric assay. As expected, b...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.05048-0
更新日期:2003-03-01 00:00:00
abstract::A total of 69 strains of Escherichia coli from patients in the Taizhou Municipal Hospital, China, were isolated, and 11 strains were identified that were resistant to bacitracin, chloramphenicol, tetracycline and erythromycin. These strains were PCR positive for at least two out of three genes, ybjG, dacC and mdfA, by...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.062893-0
更新日期:2013-11-01 00:00:00
abstract::Bovine lactoferricin, a pepsin-generated antimicrobial peptide from bovine lactoferrin active against a wide range of bacteria, was tested for its ability to influence the adhesion and invasion of Yersinia enterocolitica and Yersinia pseudotuberculosis in HEp-2 cells. The addition of non-cytotoxic and non-bactericidal...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.05410-0
更新日期:2004-05-01 00:00:00
abstract::Clostridium difficile-associated diarrhoea (CDAD) is the most common hospital-acquired diarrhoea, and is a major type of gastroenteritis infection in nursing homes and facilities for the elderly. In this study the antimicrobial activity of the two-component lantibiotic, lacticin 3,147, against a range of genetically d...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.47085-0
更新日期:2007-07-01 00:00:00
abstract::There is a paucity of information about the factors associated with oral colonization with Candida species and the changes associated with antiretroviral (ARV) therapy. This study investigated the role of ARV therapy and other factors in a study population. Relevant clinical and laboratory information was obtained and...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.047522-0
更新日期:2013-01-01 00:00:00
abstract::Iron is an important nutritional requirement for bacteria due to its conserved role in many essential metabolic processes. As a consequence of the lack of freely available iron in the mammalian host, bacteria upregulate a range of virulence factors during infection. Transcriptional analysis of Francisella tularensis s...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.47190-0
更新日期:2007-10-01 00:00:00
abstract::Francisella tularensis has been recognized as the causative agent of tularaemia for almost a century. Since its discovery in 1911, it has been shown to infect a wide range of hosts, including humans. As early as the 1920s it was suggested to be an intracellular pathogen, but it has proven to be an enigmatic organism, ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章,评审
doi:10.1099/jmm.0.2008/000653-0
更新日期:2008-08-01 00:00:00