Ultrastructure of Helicobacter trogontum in culture and in the gastrointestinal tract of gnotobiotic mice.

Abstract:

:Helicobacter trogontum is a micro-aerophilic urease-positive bacterium that has recently been isolated from the intestinal mucosa of rats. The purpose of this investigation was to study the ultrastructural details of this micro-organism in both pure culture and in the gastrointestinal tract of germ-free mice infected with H. trogontum. The micro-organism was a fusiform to slightly spiral gram-negative cell, 4-6 microm long and 0.6-0.7 microm wide, with four to seven bipolar sheathed flagella. The cytoplasm presented several irregular and also globular granules. On each side of the polar regions of the cells, there was a highly electron-dense band, the 'polar membrane'. Coccoidal forms were seen in old cultures. H. trogontum showed several ultrastructural characteristics of the Helicobacter genus and much resemblance to H. rappini and H. bilis. H. trogontum mainly colonised the large bowel of the gnotobiotic mice where it could be seen in the lumen and also inside the enterocytes. Vacuolation of the ileal epithelial cells, loss of microvilli and pronounced desquamation of the enterocytes of the caecum were observed in the bowel colonised by the bacterium. These observations raise the possibility that H. trogontum could cause some harm to the host at least in particular circumstances such as when it colonises the gastrointestinal tract of a germ-free host.

journal_name

J Med Microbiol

authors

Moura SB,Mendes EN,Queiroz DM,Camargos ER,Evangelina M,Fonseca F,Rocha GA,Nicoli JR

doi

10.1099/00222615-47-6-513

subject

Has Abstract

pub_date

1998-06-01 00:00:00

pages

513-20

issue

6

eissn

0022-2615

issn

1473-5644

journal_volume

47

pub_type

杂志文章
  • A particle agglutination assay for rapid identification of heparin binding to coagulase-negative staphylococci.

    abstract::The heparin-binding properties of six different species of coagulase-negative staphylococci were examined by a particle agglutination assay. Heparin (mol. wt 4000-6000), mildly treated with sodium periodate, was covalently coupled to amino-modified latex beads (0.72 micron diameter). The particle agglutination assay w...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-45-4-263

    authors: Pascu C,Hirmo S,Ljungh A,Wadström T

    更新日期:1996-10-01 00:00:00

  • Antifungal susceptibilities, biofilms, phospholipase and proteinase activities in the Candida rugosa complex and Candida pararugosa isolated from tertiary teaching hospitals.

    abstract:PURPOSE:Non-albicansCandida species have emerged as fungal pathogens that cause invasive infections, with many of these species displaying resistance to commonly used antifungal agents. This study was confined to studying the characteristics of clinical isolates of the C. rugosa complex and C. pararugosa species. METH...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000940

    authors: Peremalo T,Madhavan P,Hamzah S,Than L,Wong EH,Nasir MDM,Chong PP,Ng KP

    更新日期:2019-03-01 00:00:00

  • RNA-dependent DNA-polymerase activity in human milk.

    abstract::A simple method is described for testing milk specimens from nursing mothers for the presence of RNA-dependent DNA-polymerase activity. Positive results were obtained in five of 137 women (3.6%) without a family history of breast cancer, and in six of 31 women (19.3%) with a family history of breast cancer. ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-8-3-447

    authors: McFarlane ES,Ryan SM,Mann E

    更新日期:1975-08-01 00:00:00

  • Analysis of methods commonly used for glycopeptide and oxazolidinone susceptibility testing in Enterococcus faecium isolates.

    abstract::The susceptibility to teicoplanin, vancomycin and linezolid of 30 clinical isolates of Enterococcus faecium was tested by Vitek 2, Phoenix, Etest, broth microdilution and disc diffusion tests. The vanA and vanB resistance genes and the 23S rRNA gene G2576T mutation were detected by PCR and PCR-RFLP, respectively. Resi...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.016444-0

    authors: Raponi G,Ghezzi MC,Gherardi G,Lorino G,Dicuonzo G

    更新日期:2010-06-01 00:00:00

  • An outbreak of Shigella boydii serotype 20 in January 2015 amongst United Kingdom healthcare workers involved in the Ebola response in Sierra Leone.

    abstract::In January 2015, Public Health England and the United Kingdom (UK) Ministry of Defence investigated cases of diarrhoea and fever in military personnel recently returned to the UK after supporting the response to the Ebola epidemic in Sierra Leone. Tests for Ebola virus infection were negative. PCR tests detected the i...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000832

    authors: Todkill D,Pudney R,Terrell A,Tuck J,Hutley E,Dallman TJ,Jenkins C,Manuel R,Wade M,Puleston R,Hawker J

    更新日期:2018-11-01 00:00:00

  • Accuracy of in vitro susceptibility tests for carbapenemase-producing Gram-negative bacteria.

    abstract::Accurate susceptibility results on antibiotic-resistant bacteria are essential for proper treatment of infections. In this study, 100 metallo-β-lactamase (MBL)-producing strains and 95 isolates with Klebsiella pneumoniae carbapenemase (KPC) were tested for carbapenem susceptibility using two automated platforms, the P...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000067

    authors: Sun J,Xu Y,Yu Y,Ni Y

    更新日期:2015-06-01 00:00:00

  • Comparison of utility of blood cultures from intravascular catheters and peripheral veins: a systematic review and decision analysis.

    abstract::Blood cultures are sometimes obtained from intravascular catheters for convenience. However, there is controversy regarding this practice. The authors compared the diagnostic test characteristics of blood cultures obtained from intravascular catheters and peripheral veins. Relevant studies for inclusion in this review...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章,评审

    doi:10.1099/jmm.0.47432-0

    authors: Falagas ME,Kazantzi MS,Bliziotis IA

    更新日期:2008-01-01 00:00:00

  • Multi-drug resistant Vibrio cholerae O1 variant El Tor isolated in northern Vietnam between 2007 and 2010.

    abstract::Since 2007, there has been a re-emergence of cholera outbreaks in northern Vietnam. To understand the molecular epidemiological relatedness and determine the antibiotic susceptibility profiles of responsible V. cholerae O1 outbreak strains, a representative collection of 100 V. cholerae O1 strains was characterized. V...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.034744-0

    authors: Tran HD,Alam M,Trung NV,Van Kinh N,Nguyen HH,Pham VC,Ansaruzzaman M,Rashed SM,Bhuiyan NA,Dao TT,Endtz HP,Wertheim HFL

    更新日期:2012-03-01 00:00:00

  • Denaturing gradient gel electrophoresis analysis for the detection of point mutations in the Chlamydia trachomatis major outer-membrane protein gene.

    abstract::Fifty clinical strains of Chlamydia trachomatis were studied by denaturing gradient gel electrophoresis (DGGE) of bacterial DNA amplified by the polymerase chain reaction (PCR). The strains belonged to the three most commonly encountered serovars in developed countries--D, E and F. Six reference strains, including the...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-43-1-14

    authors: Sayada C,Denamur E,Grandchamp B,Orfila J,Elion J

    更新日期:1995-07-01 00:00:00

  • Comparison of PCR, culturing and Pap smear microscopy for accurate diagnosis of genital Actinomyces.

    abstract::Members of the genus Actinomyces, Gram-positive, non-spore-forming anaerobic bacteria, are normal inhabitants of the mucosal surfaces of the oral, gastrointestinal and genital tracts. Identification of these bacteria using conventional methods is generally difficult because of their complex transport and growth requir...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.053165-0

    authors: Kaya D,Demirezen Ş,Hasçelik G,Gülmez Kıvanç D,Beksaç MS

    更新日期:2013-05-01 00:00:00

  • Induction of mutation in Staphylococcus aureus by ethylmethane sulphonate.

    abstract::Staphylococcus aureus strains were treated with ethylmethane sulphonate with the object of obtaining mutants that differed from the wild type in one of the properties that might contribute to virulence. Mutants with isolated loss or deficient production of gamma-haemolysin, coagulase, leucocidin and staphylokinase wer...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-8-2-265

    authors: van der Vijver JC,van Es-Boon MM,Michel MF

    更新日期:1975-05-01 00:00:00

  • Exceptionally high titres in atypical presentation of occult epididymo-orchitis due to brucellosis.

    abstract::A male patient of 32 years was referred for surgical drainage and orchidectomy of the right testis following a cycling injury. A Venereal Disease Research Laboratory (VDRL) test was requested by the surgery department to rule out secondary syphilis. Although serum samples gave a negative result in the VDRL test, quali...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.034892-0

    authors: Purwar S,Metgud SC,Gokale SK

    更新日期:2012-03-01 00:00:00

  • An experimental model of gastrointestinal candidiasis.

    abstract::Intestinal absorption of arbutin (p-hydroxyphenyl-beta-glucoside), a non-metabolised analogue of d-glucose, and net flux of water out of the small intestinal lumen were found to be decreased in vitro in rats fed with Candida albicans. In rats on a protein-deficient diet and fed C. albicans there was net secretion of w...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-13-1-103

    authors: Burke V,Gracey M

    更新日期:1980-02-01 00:00:00

  • Human herpesvirus 6: molecular biology and clinical features.

    abstract::Human herpesvirus 6 (HHV-6) exists as distinct variants HHV-6A and HHV-6B. The complete genomes of HHV-6A and HHV-6B have been sequenced. HHV-6B contains 97 unique genes. CD46 is the cell receptor for HHV-6, explaining its broad tissue tropism but its restricted host-species range. HHV-6 utilizes a number of strategie...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章,评审

    doi:10.1099/jmm.0.05074-0

    authors: Dockrell DH

    更新日期:2003-01-01 00:00:00

  • Effect of carbapenems on the transcriptional expression of the oprD, oprM and oprN genes in Pseudomonas aeruginosa.

    abstract::The effects of imipenem and meropenem on the transcriptional expression of resistance-related genes oprD, oprM and oprN in Pseudomonas aeruginosa were studied by quantitative real-time PCR. Four strains were examined: the type strain PT5 (PAO1), its derivatives M7 and PT149, and a clinical isolate, PaKT3. The derivati...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.45692-0

    authors: Kolayli F,Karadenizli A,Savli H,Ergen K,Hatirnaz O,Balikci E,Budak F,Vahaboglu H

    更新日期:2004-09-01 00:00:00

  • Factors determining the susceptibility of mice to experimental phycomycosis.

    abstract::Various factors influencing the susceptibility of C3H mice to lethal infection by the phycomycete Absidia ramosa were examined. Mice 19-21 days old were exposed to graded doses of A. ramosa spores by various routes. After intravenous inoculation with doses in excess of 10(3) viable units, a variable proportion of mice...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-8-4-551

    authors: Corbel MJ,Eades SM

    更新日期:1975-11-01 00:00:00

  • Identification by PCR of Helicobacter pylori in subgingival plaque of adult periodontitis patients.

    abstract::The PCR was used to detect the presence of Helicobacter pylori in subgingival plaque samples from patients with adult periodontitis. Primers based upon the 16S ribosomal RNA (rRNA) gene sequence of H. pylori were used in a single round of PCR to amplify a 295-bp DNA fragment and the identity of the amplified products ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-48-3-317

    authors: Riggio MP,Lennon A

    更新日期:1999-03-01 00:00:00

  • Moraxella (Branhamella) catarrhalis--clinical and molecular aspects of a rediscovered pathogen.

    abstract::Since its discovery at the end of the nineteenth century, Moraxella (Branhamella) catarrhalis has undergone several changes of nomenclature and periodic changes in its perceived status as either a commensal or a pathogen. Molecular analysis based on DNA hybridisation or 16S rDNA sequence comparisons has established it...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章,评审

    doi:10.1099/00222615-46-5-360

    authors: Enright MC,McKenzie H

    更新日期:1997-05-01 00:00:00

  • Characterisation of Eubacterium-like strains isolated from oral infections.

    abstract::The genus Eubacterium currently includes a heterogeneous group of gram-positive, non-spore-forming anaerobic bacilli, many of which are slow growing, fastidious and generally unreactive in biochemical tests. As a consequence, cultivation and identification of isolates are difficult and the taxonomy of the group remain...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-50-11-947

    authors: Downes J,Munson MA,Spratt DA,Kononen E,Tarkka E,Jousimies-Somer H,Wade WG

    更新日期:2001-11-01 00:00:00

  • Nasal colonization by four potential respiratory bacteria in healthy children attending kindergarten or elementary school in Seoul, Korea.

    abstract::A longitudinal analysis was carried out of the colonization by four potential respiratory pathogens - Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis and Staphylococcus aureus - in 165 healthy children (aged 3-7 years) attending three kindergartens and 417 healthy children (aged 7-10 years) att...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.040584-0

    authors: Bae S,Yu JY,Lee K,Lee S,Park B,Kang Y

    更新日期:2012-05-01 00:00:00

  • Microbial profiling of dental plaque from mechanically ventilated patients.

    abstract::Micro-organisms isolated from the oral cavity may translocate to the lower airways during mechanical ventilation (MV) leading to ventilator-associated pneumonia (VAP). Changes within the dental plaque microbiome during MV have been documented previously, primarily using culture-based techniques. The aim of this study ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000212

    authors: Sands KM,Twigg JA,Lewis MAO,Wise MP,Marchesi JR,Smith A,Wilson MJ,Williams DW

    更新日期:2016-02-01 00:00:00

  • Co-elimination of mec and spa genes in Staphylococcus aureus and the effect of agr and protein A production on bacterial adherence to cell monolayers.

    abstract::Phenotypic loss of protein A production was tested in six methicillin-resistant (McR) Staphylococcus aureus (MRSA) isolates and their isogenic methicillin-sensitive (McS) variants by a radiolabelled IgG-binding assay with washed cells and by Western blotting of supernates prepared from lysed washed cells. Genomic DNA ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-39-6-422

    authors: Poston SM,Glancey GR,Wyatt JE,Hogan T,Foster TJ

    更新日期:1993-12-01 00:00:00

  • In vitro efficacy of sodium selenite in reducing toxin production, spore outgrowth and antibiotic resistance in hypervirulent Clostridium difficile.

    abstract:PURPOSE:This study investigated the efficacy of the essential mineral, selenium (sodium selenite), in reducing the toxin production, spore outgrowth and antibiotic resistance of Clostridium difficile in vitro. METHODOLOGY:Two hypervirulent C. difficile isolates were cultured in brain heart infusion broth with and with...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.001008

    authors: Pellissery AJ,Vinayamohan PG,Yin HB,Mooyottu S,Venkitanarayanan K

    更新日期:2019-07-01 00:00:00

  • Antimicrobial resistance profiling and molecular typing of methicillin-resistant Staphylococcus aureus isolated from a Malaysian teaching hospital.

    abstract::The annual prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in Malaysia has been estimated to be 30 % to 40 % of all S. aureus infections. Nevertheless, data on the antimicrobial resistance and genetic diversity of Malaysian MRSAs remain few. In 2009, we collected 318 MRSA strains from various wards of...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000387

    authors: Noordin A,Sapri HF,Mohamad Sani NA,Leong SK,Tan XE,Tan TL,Mohamad Zin N,Neoh HM,Hussin S

    更新日期:2016-12-01 00:00:00

  • Antibodies to Shiga toxin in the serum of children with Shigella-associated haemolytic uraemic syndrome.

    abstract::Antibodies to Shiga toxin (Stx) were measured in the sera of 49 children with Shigella dysenteriae serotype 1 infection, of whom 17 had haemolytic uraemic syndrome (HUS) and 32 had no complications (uncomplicated shigellosis, UCS). Children with HUS had lower levels of total IgG and IgM and lower IgM titres to Stx tha...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-48-1-11

    authors: Azim T,Rashid A,Qadri F,Sarker MS,Hamadani J,Salam MA,Wahed MA,Albert MJ

    更新日期:1999-01-01 00:00:00

  • Characteristics of a pandemic clone of O3 : K6 and O4 : K68 Vibrio parahaemolyticus isolated in Beira, Mozambique.

    abstract::The genetic characteristics of Vibrio parahaemolyticus strains isolated in 2004 and 2005 in Mozambique were assessed in this study to determine whether the pandemic clone of V. parahaemolyticus O3 : K6 and O4 : K68 serotypes has spread to Mozambique. Fifty-eight V. parahaemolyticus strains isolated from hospitalized d...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.2008/004275-0

    authors: Ansaruzzaman M,Chowdhury A,Bhuiyan NA,Sultana M,Safa A,Lucas M,von Seidlein L,Barreto A,Chaignat CL,Sack DA,Clemens JD,Nair GB,Choi SY,Jeon YS,Lee JH,Lee HR,Chun J,Kim DW

    更新日期:2008-12-01 00:00:00

  • Bacterial growth and toxin production in ileostomy effluents.

    abstract::Escherichia coli (2), Vibrio cholerae (2) and Aeromonas sobria (1) strains were examined for their ability to grow and produce toxins in samples of ileostomy fluid. Three categories of response were observed: no detectable growth, growth without detectable toxin, and growth with detectable toxin. Clear differences wer...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-28-4-287

    authors: Ala Aldeen DA,Barer MR

    更新日期:1989-04-01 00:00:00

  • Involvement of efflux mechanisms in biocide resistance of Campylobacter jejuni and Campylobacter coli.

    abstract::Active efflux has an important role in the antimicrobial resistance of Campylobacter jejuni and Campylobacter coli. The effects of two putative efflux pump inhibitors (EPIs), phenylalanine-arginine β-naphthylamide and 1-(1-naphthylmethyl)-piperazine, and the effects of inactivation of the cmeB,cmeF and cmeR genes on r...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.041467-0

    authors: Mavri A,Smole Možina S

    更新日期:2012-06-01 00:00:00

  • The host immune response to Clostridium difficile.

    abstract::Clostridium difficile is the most common cause of nosocomial bacterial diarrhoea in the Western world. Diarrhoea and colitis are caused by the actions of toxins A and B released by pathogenic strains of C. difficile. Adaptive immune responses to these toxins influence the outcomes of C. difficile infection (CDI). Symp...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章,评审

    doi:10.1099/jmm.0.030015-0

    authors: Kelly CP,Kyne L

    更新日期:2011-08-01 00:00:00

  • New assay for Gardnerella vaginalis loads correlates with Nugent scores and has potential in the diagnosis of bacterial vaginosis.

    abstract::Gardnerella vaginalis is a Gram-variable anaerobic bacterium present in 100% of women with bacterial vaginosis (BV). BV is a complex polymicrobial condition with no single causative agent. The current laboratory detection method for BV relies on a Gram-stain Nugent score to estimate the quantity of different bacterial...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000118

    authors: Cox C,McKenna JP,Watt AP,Coyle PV

    更新日期:2015-09-01 00:00:00