Abstract:
:A total of 69 strains of Escherichia coli from patients in the Taizhou Municipal Hospital, China, were isolated, and 11 strains were identified that were resistant to bacitracin, chloramphenicol, tetracycline and erythromycin. These strains were PCR positive for at least two out of three genes, ybjG, dacC and mdfA, by gene mapping with conventional PCR detection. Conjugation experiments demonstrated that these genes existed in plasmids that conferred resistance. Novel ybjG and dacC variants were isolated from E. coli strains EC2163 and EC2347, which were obtained from the sputum of intensive care unit patients. Genetic mapping showed that the genes were located on 8200 kb plasmid regions flanked by EcoRI restriction sites. Three distinct genetic structures were identified among the 11 PCR-positive strains of E. coli, and two contained the novel ybjG and dacC variants. The putative amino acid differences in the ybjG and dacC gene variants were characterized. These results provide evidence for novel variants of ybjG and dacC, and suggest that multiple drug resistance in hospital strains of E. coli depends on the synergistic function of ybjG, dacC and mdfA within three distinct genetic structures in conjugative plasmids.
journal_name
J Med Microbioljournal_title
Journal of medical microbiologyauthors
Wang D,Hu E,Chen J,Tao X,Gutierrez K,Qi Ydoi
10.1099/jmm.0.062893-0subject
Has Abstractpub_date
2013-11-01 00:00:00pages
1728-1734issue
Pt 11eissn
0022-2615issn
1473-5644journal_volume
62pub_type
杂志文章abstract::The family of leptospiral immunoglobulin-like (lig) genes comprises ligA, ligB and ligC. This study used PCR to demonstrate the presence of lig genes among serovars from a collection of leptospiral strains and clinical isolates. Whilst ligA and ligC appeared to be present in a limited number of pathogenic serovars, th...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.009175-0
更新日期:2009-09-01 00:00:00
abstract:PURPOSE:Group A rotavirus (RVA) is the leading cause of severe gastroenteritis in children younger than 5 years. The most common human G-types are G1-4 and G9. G12 genotype is currently emerging worldwide, becoming the sixth most prevalent RVA G-genotype. In Tunisia, an emergence of G12 RVA strains was observed. To und...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000420
更新日期:2017-02-01 00:00:00
abstract::Synergy between fluvastatin, at clinically unachievable concentrations, and fluconazole against Candida albicans has been reported. The purpose of the present study was to evaluate the in-vitro activity of fluconazole alone and in combination with clinically achievable concentrations of pravastatin and fluvastatin aga...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/0022-1317-51-2-105
更新日期:2002-02-01 00:00:00
abstract::Interaction of human polymorphonuclear leucocytes (PMNL) and influenza virus (IFV) was studied in vitro. At 0 degree C, the viral particles were bound extensively to the surface of the PMNL membrane with a ratio of about 1000 virus particles to a single PMNL. The binding was sensitive to neuraminidase, suggesting atta...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-28-3-191
更新日期:1989-03-01 00:00:00
abstract::Of 166 strains of Staphylococcus aureus isolated from hospital patients and staff in Ile-Ife, Nigeria, 32% were non-typable (NT). The strains were isolated from skin lesions, eye infections, wounds and nasal flora. Isolates from skin lesions or eye and wound infections were more likely to produce protease (95% and 78%...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-34-2-109
更新日期:1991-02-01 00:00:00
abstract::The ability of Mycobacterium leprae to specifically bind alpha2-laminins of Schwann cells has been described recently as being an important property of the leprosy bacillus, which could explain the neural tropism of M. leprae. Therefore, the extent of the expression of alpha2-laminin-binding properties among mycobacte...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/0022-1317-50-1-23
更新日期:2001-01-01 00:00:00
abstract::Members of the Burkholderia cepacia complex are important bacterial pathogens in cystic fibrosis (CF) patients. The B. cepacia complex currently consists of nine genetic subgroups (genomovars) of different epidemiological relevance and possibly of different pathogenic potential in humans. In this study, a new approach...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.46457-0
更新日期:2006-06-01 00:00:00
abstract::Francisella tularensis is a highly virulent intracellular bacterium capable of rapid multiplication in phagocytic cells. Previous studies have revealed that activation of F. tularensis-infected macrophages leads to control of infection and reactive nitrogen and oxygen species make important contributions to the bacter...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.032870-0
更新日期:2011-11-01 00:00:00
abstract::This study was undertaken to investigate the synergistic interaction between amphotericin B (AmB) and acteoside, isolated from the aerial parts of the shrub Colebrookea oppositifolia (Lamiaceae). Acteoside alone exhibited no intrinsic antifungal activity but showed a potent synergism in combination with AmB against se...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.031906-0
更新日期:2011-09-01 00:00:00
abstract::Antibiotic-resistant bacteria have emerged from the widespread use of antibiotics worldwide and have prompted the search for new sources of antimicrobial substances. Pinus spp. contain several bioactive compounds consisting mainly of terpenes, terpenoids and some other aromatic and aliphatic constituents. These compou...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.081711-0
更新日期:2014-12-01 00:00:00
abstract::Shiga toxins (Stx1 and Stx2) are responsible for initiating haemolytic uraemic syndrome, a serious extraintestinal complication caused by enterohaemorrhagic Escherichia coli O157 : H7 infection in humans. Shiga toxins are classical AB(5)-type exotoxins, consisting of a globotriaosylceramide (Gb(3))-binding B subunit p...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.47282-0
更新日期:2007-11-01 00:00:00
abstract::Mice were vaccinated with lipopolysaccharide (LPS) from Leptospira interrogans serovar pomona or hardjo, or with the polysaccharide (PS) fraction of the LPS, or with an immunoconjugate of PS and diphtheria toxoid (DT). Maximum agglutinin titres were found 6-10 weeks after vaccination with LPS or PS-DT conjugate; the l...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-33-3-199
更新日期:1990-11-01 00:00:00
abstract::Introduction. Melioidosis, caused by Burkholderia pseudomallei, in endemic areas, poses a challenge for treating the diseased populations without accurate diagnosis, and the disease-specific biomarkers linked with the infection have yet to be reported. Due to the invasive nature of the causative agent, Burkholderia ps...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.001242
更新日期:2020-10-01 00:00:00
abstract::Fifteen randomly selected nasopharyngeal (NP) swab specimens (culture-negative for influenza A virus) were spiked with influenza A virus and the nucleic acids were extracted and subjected to PCR amplification with Thermus aquaticus (Taq) and T. thermophilus (Tth) DNA polymerases. Products of the expected size, and giv...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-47-12-1131
更新日期:1998-12-01 00:00:00
abstract:PURPOSE:Conventional laboratory detection methods for gastrointestinal parasites are time consuming, require considerable technical expertise and may suffer from poor analytical sensitivity. This study sought to evaluate the automated BD MAX Enteric Parasite Panel (EPP) for the detection of Cryptosporidium parvum/homin...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.000558
更新日期:2017-08-01 00:00:00
abstract::The genetic characteristics of Vibrio parahaemolyticus strains isolated in 2004 and 2005 in Mozambique were assessed in this study to determine whether the pandemic clone of V. parahaemolyticus O3 : K6 and O4 : K68 serotypes has spread to Mozambique. Fifty-eight V. parahaemolyticus strains isolated from hospitalized d...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.2008/004275-0
更新日期:2008-12-01 00:00:00
abstract::Candida albicans is the species most frequently isolated from oral specimens, but the recovery of other Candida species such as Candida dubliniensis is increasing. Differentiation of C. dubliniensis from C. albicans requires special tests and both species are misidentified in some studies. CHROM-Pal (CH-P) is a novel ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.011320-0
更新日期:2009-11-01 00:00:00
abstract::Cholera, caused by Vibrio cholerae, results in significant morbidity and mortality worldwide, including Thailand. Representative V. cholerae strains associated with endemic cholera (n = 32), including strains (n = 3) from surface water sources, in Khon Kaen, Thailand (2003-2011), were subjected to microbiological, mol...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.053801-0
更新日期:2013-04-01 00:00:00
abstract::We have characterised 45 isolates of methicillin-resistant Staphylococcus aureus (MRSA) from Glasgow Royal Infirmary by means of simple biotyping, immunoblotting of exported proteins and restriction enzyme fragmentation patterns (REFP) of plasmid DNA. The strains were subdivided into four groups (A-D) on the basis of ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-31-2-125
更新日期:1990-02-01 00:00:00
abstract::Sorbitol-MacConkey medium has become widely used for the isolation of verotoxigenic (VT+) Escherichia coli O157. However, many organisms other than VT+ E. coli O157, especially other serogroups of E. coli and Proteus spp., may not ferment sorbitol, and thus may be confused initially with VT+ E. coli O157. Rhamnose is ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-35-2-107
更新日期:1991-08-01 00:00:00
abstract::A rapid and simple method for counting viable bacteria is described. The technique involves the use of a semi-automatic micropipette and disposable glass-capillary tubes. Viable counts obtained by this method were not significantly different from those obtained by the Miles, Misra and Irwin method or by a pour-plate m...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-11-4-541
更新日期:1978-11-01 00:00:00
abstract::The plasmid profiles, and their association with antimicrobial resistance, of 60 strains of Aeromonas hydrophila isolated from fish, shellfish and water were investigated. Only two strains were susceptible to all the antimicrobial agents tested; the highest incidences of resistance were to tetracycline (96.7%), prysta...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-35-5-264
更新日期:1991-11-01 00:00:00
abstract::This case report describes a case of nuclear bilateral Bell's palsy and ageusia associated with Mycoplasma pneumoniae infection. Magnetic resonance imaging evidenced T2-weighted hyper-intense protuberantial lesions. Such topography leading to a nuclear palsy contrasts with previously reported infectious diplegia invol...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.45859-0
更新日期:2005-04-01 00:00:00
abstract::Rapid identification of the causative bacteria of sepsis in patients can contribute to the selection of appropriate antibiotics and improvement of patients' prognosis. Genotypic identification is an emerging technology that may provide an alternative method to, or complement, established phenotypic identification proc...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.049163-0
更新日期:2012-11-01 00:00:00
abstract::Francisella tularensis has been recognized as the causative agent of tularaemia for almost a century. Since its discovery in 1911, it has been shown to infect a wide range of hosts, including humans. As early as the 1920s it was suggested to be an intracellular pathogen, but it has proven to be an enigmatic organism, ...
journal_title:Journal of medical microbiology
pub_type: 杂志文章,评审
doi:10.1099/jmm.0.2008/000653-0
更新日期:2008-08-01 00:00:00
abstract::The growth inhibiting activity of transferrins, citrate, 2-2' dipyridyl and desferrioxamine methanesulphonate towards Legionella spp. and their serogroups was investigated. The inhibitory activity of all these compounds depended upon the iron-free state of the molecules and was abolished by saturation with iron. No ba...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-34-2-113
更新日期:1991-02-01 00:00:00
abstract::The move towards pathology networks and hub-and-spoke models of medical laboratory service provision has significantly changed the flow of samples, and the impact of results on patients, over recent years. At the same time advances in technology, including rapid, simple to use molecular platforms, are changing the way...
journal_title:Journal of medical microbiology
pub_type: 信件
doi:10.1099/jmm.0.000919
更新日期:2019-03-01 00:00:00
abstract::To study the influence of normal associated microbiota on systemic immunological responses during experimental Chagas' disease, germ-free and conventional NIH Swiss mice were infected with Y strain of Trypanosoma cruzi. Although no statistical differences in mortality and parasitaemia were found, conventional mice sho...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/jmm.0.45657-0
更新日期:2004-08-01 00:00:00
abstract::Clostridium piliforme is an obligate intracellular bacterium that causes enterohepatic disease in many animal species. C. piliforme infections are commonly subclinical in laboratory rats and mice, and little is known about host regulation of disease or of the effects of C. piliforme infections on investigations that u...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/0022-1317-49-6-557
更新日期:2000-06-01 00:00:00
abstract::The opsonic and protective capacities of rabbit antisera against Escherichia coli O, K and core-glycolipid cell-wall antigens were compared with specific antibody titres as measured by agglutination and enzyme-linked immunosorbent assay. Anti-O antisera were opsonic and protective against two noncapsulate strains. Onl...
journal_title:Journal of medical microbiology
pub_type: 杂志文章
doi:10.1099/00222615-14-4-381
更新日期:1981-11-01 00:00:00