Induction of granulomas in interferon-gamma gene-disrupted mice by avirulent but not by virulent strains of Mycobacterium tuberculosis.

Abstract:

:To gain a better understanding of the pathological role of interferon-gamma (IFN-gamma) in specific granuloma formation, IFN-gamma gene-deficient mice (BALB/c and C57BL/6) were produced. The IFN-gamma gene in embryonic stem (ES) cells was disrupted by inserting the beta-galactosidase gene (lacZ) and the neomycin resistance gene (neo) at the translation initiation site in exon 1 by homologous recombination. Six-week-old IFN-gamma-deficient and wild-type mice were inoculated with 10(3)-10(7) bacilli of various strains of Mycobacterium tuberculosis (Kurono, H37Rv, H37Ra and BCG Pasteur) through their tail veins. The mice were examined 7 weeks later for granuloma formation. The avirulent BCG Pasteur and H37Ra strains (10(3)-10(4) bacilli/ml) induced granulomas in the spleen, liver and lungs of IFN-gamma-deficient mice. The granulomas consisted of epithelioid macrophages and Langhans multinucleate giant cells, but lacked caseous necrosis. The virulent Kurono and H37Rv strains induced disseminated abscesses but not granulomas in various organs of IFN-gamma-deficient mice and Mac-3-positive macrophages were not detected in the abscess lesions. These results suggest that IFN-gamma may be primarily responsible for macrophage activation and that other factor(s) may be involved in the granuloma formation mechanism.

journal_name

J Med Microbiol

authors

Sugawara I,Yamada H,Kazumi Y,Doi N,Otomo K,Aoki T,Mizuno S,Udagawa T,Tagawa Y,Iwakura Y

doi

10.1099/00222615-47-10-871

subject

Has Abstract

pub_date

1998-10-01 00:00:00

pages

871-7

issue

10

eissn

0022-2615

issn

1473-5644

journal_volume

47

pub_type

杂志文章
  • Identification and characterization of a vancomycin-resistant Staphylococcus aureus isolated from Kolkata (South Asia).

    abstract::A pathogenic vancomycin-resistant Staphylococcus aureus (VRSA) isolate (MIC > or =64 microg ml(-1)) was obtained from a Kolkata hospital in June 2005. Species identification was confirmed by Gram staining, standard biochemical tests and PCR amplification of the nuc gene, which encodes the thermostable nuclease that is...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.47144-0

    authors: Saha B,Singh AK,Ghosh A,Bal M

    更新日期:2008-01-01 00:00:00

  • Use of hamster antisera in the preliminary differentiation of Leptospira interrogans servars hardjo and balcanica.

    abstract::Preliminary differentiation of Leptospira interrogans serovars hardjo and balcanica can be made with antisera that are collected from hamsters after experimental infection with serovar hardjo and selected for high homologous and low or nil heterologous titres, as measured by the microscopic agglutination test. Rabbit ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-11-3-351

    authors: Ris DR,Hamel KL

    更新日期:1978-08-01 00:00:00

  • Cryptic plasmids in hospital isolates of Providencia stuarti.

    abstract::The distribution of cryptic plasmids among 123 isolates of Providencia stuarti from a hospital ward during a prospective epidemiological study is reported. Two closely related stable plasmids (34 Kb and 36 Kb) were identified by restriction endonuclease digest analysis of plasmid DNA. One or other of these cryptic pla...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-18-2-277

    authors: Hawkey PM,Bennett PM,Hawkey CA

    更新日期:1984-10-01 00:00:00

  • Bacterial growth and toxin production in ileostomy effluents.

    abstract::Escherichia coli (2), Vibrio cholerae (2) and Aeromonas sobria (1) strains were examined for their ability to grow and produce toxins in samples of ileostomy fluid. Three categories of response were observed: no detectable growth, growth without detectable toxin, and growth with detectable toxin. Clear differences wer...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-28-4-287

    authors: Ala Aldeen DA,Barer MR

    更新日期:1989-04-01 00:00:00

  • The characterisation of clinical isolates of Staphylococcus aureus in Ile-Ife, Nigeria.

    abstract::Of 166 strains of Staphylococcus aureus isolated from hospital patients and staff in Ile-Ife, Nigeria, 32% were non-typable (NT). The strains were isolated from skin lesions, eye infections, wounds and nasal flora. Isolates from skin lesions or eye and wound infections were more likely to produce protease (95% and 78%...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-34-2-109

    authors: Ako-Nai AK,Ogunniyi AD,Lamikanra A,Torimiro SE

    更新日期:1991-02-01 00:00:00

  • Identification and comparative analysis of the lactoferrin and transferrin receptors among clinical isolates of gonococci.

    abstract::Neisseria gonorrhoeae expresses receptors for both lactoferrin (LF) and transferrin (TF). To determine whether qualitative or quantitative changes in these receptors, or both, correlate with the pattern of disease due to N. gonorrhoeae, a dot binding assay with whole cells was used to measure the absolute receptor lev...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-28-3-199

    authors: Lee BC,Bryan LE

    更新日期:1989-03-01 00:00:00

  • Streptococcus mutans infective endocarditis complicated by vertebral discitis following dental treatment without antibiotic prophylaxis.

    abstract::We report what we believe is the first reported case of Streptococcus mutans endocarditis complicated by vertebral discitis. The case is particularly interesting and topical as it occurred in a patient with pre-existing cardiac valvular disease who had recently had a dental procedure without antibiotic prophylaxis fol...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.020974-0

    authors: Biswas S,Bowler ICJW,Bunch C,Prendergast B,Webster DP

    更新日期:2010-10-01 00:00:00

  • DNA identification of the pathogen of candidal aspiration pneumonia induced in the course of oral cancer therapy.

    abstract::Aspiration of oropharyngeal bacteria and fungi is occasionally suspected in patients with pneumonia. A patient with oral carcinoma underwent chemoradioimmunotherapy and, about 4 weeks from the start of the therapy, the patient suffered from severe oral mucositis induced by chemoradiotherapy, and candidal pneumonia was...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.45769-0

    authors: Yamamoto T,Ueta E,Kamatani T,Osaki T

    更新日期:2005-05-01 00:00:00

  • Age-specific prevalence of diffusely adherent Escherichia coli in Brazilian children with acute diarrhoea.

    abstract::In a prospective study between February 2003 and June 2004, stool specimens of children less than 2 years of age with diarrhoea (n=218) and without diarrhoea (n=86), living in Vitória, Espírito Santo, Brazil, were examined for the presence of diarrhoeagenic Escherichia coli. E. coli isolates were tested by colony blot...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.47660-0

    authors: Spano LC,Sadovsky ADI,Segui PN,Saick KW,Kitagawa SMS,Pereira FEL,Fagundes-Neto U,Scaletsky ICA

    更新日期:2008-03-01 00:00:00

  • Ultrastructure of Helicobacter trogontum in culture and in the gastrointestinal tract of gnotobiotic mice.

    abstract::Helicobacter trogontum is a micro-aerophilic urease-positive bacterium that has recently been isolated from the intestinal mucosa of rats. The purpose of this investigation was to study the ultrastructural details of this micro-organism in both pure culture and in the gastrointestinal tract of germ-free mice infected ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-47-6-513

    authors: Moura SB,Mendes EN,Queiroz DM,Camargos ER,Evangelina M,Fonseca F,Rocha GA,Nicoli JR

    更新日期:1998-06-01 00:00:00

  • Identification of bacteria directly from positive blood culture samples by DNA pyrosequencing of the 16S rRNA gene.

    abstract::Rapid identification of the causative bacteria of sepsis in patients can contribute to the selection of appropriate antibiotics and improvement of patients' prognosis. Genotypic identification is an emerging technology that may provide an alternative method to, or complement, established phenotypic identification proc...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.049163-0

    authors: Motoshima M,Yanagihara K,Morinaga Y,Matsuda J,Hasegawa H,Kohno S,Kamihira S

    更新日期:2012-11-01 00:00:00

  • Identification of an immunogenic 18-kDa protein of Helicobacter pylori by alkaline phosphatase gene fusions.

    abstract::The use of alkaline phosphatase fusion methodology to identify Helicobacter pylori exported proteins enabled the identification of an open reading frame (ORF) encoding a highly immunogenic, previously uncharacterised exported protein. The predicted aminoacid sequence displays a typical N-terminal signal peptide and co...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-49-7-643

    authors: Oliaro J,Johnson RD,Chen W,Chadwick VS,Murray A

    更新日期:2000-07-01 00:00:00

  • Distribution of Serratia marcescens serotypes in cancer patients.

    abstract::A study of 1314 patients with malignancies was made to determine the prevalence of Serratia marcescens in surveillance, diagnostic, and environmental cultures. Sera obtained from a commercial source were used to determine serotypic distributions of the S. marcescens strains isolated during a 51-month period. S. marces...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-13-2-333

    authors: Young VM,Moody MR,Morris MJ

    更新日期:1980-05-01 00:00:00

  • Borderline oxacillin-resistant Staphylococcus aureus (BORSA) - a more common problem than expected?

    abstract::Borderline oxacillin-resistant Staphylococcus aureus (BORSA) represents a quite poorly understood and inadequately defined phenotype of methicillin resistance. BORSA strains show low, borderline resistance to penicillinase-resistant penicillins (PRPs), with oxacillin MICs typically equal to 1-8 µg ml-1, and in contras...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章,评审

    doi:10.1099/jmm.0.000585

    authors: Hryniewicz MM,Garbacz K

    更新日期:2017-10-01 00:00:00

  • Linkage between toxin production and purine biosynthesis in Clostridium difficile.

    abstract::The production of toxins A and B by Clostridium difficile was greatly enhanced under biotin-limited conditions, in which a 140-kDa protein was expressed strongly. Gene cloning revealed that this protein was a homologue of formylglycinamidine ribonucleotide synthetase (FGAM synthetase, EC 6.3.5.3), which is known as Pu...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-51-1-34

    authors: Maegawa T,Karasawa T,Ohta T,Wang X,Kato H,Hayashi H,Nakamura S

    更新日期:2002-01-01 00:00:00

  • Candida krusei: biology, epidemiology, pathogenicity and clinical manifestations of an emerging pathogen.

    abstract::Early reports of Candida krusei in man describe the organism as a transient, infrequent isolate of minor clinical significance inhabiting the mucosal surfaces. More recently it has emerged as a notable pathogen with a spectrum of clinical manifestations such as fungaemia, endophthalmitis, arthritis and endocarditis, m...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章,评审

    doi:10.1099/00222615-41-5-295

    authors: Samaranayake YH,Samaranayake LP

    更新日期:1994-11-01 00:00:00

  • Studies on the Vibrio cholerae mucinase complex. III. Neutralisation of the neuraminidase activity by specific anti-neuraminidase IgG.

    abstract::A partially-purified neuraminidase from the mucinase complex of Vibrio cholerae was used to prepare a specific anti-neuraminidase antiserum in rabbits. When the neutralising potency of this serum against V. cholerae neuraminidase was assessed in conventional tests, the enzymic activity, as measured by thiobarbituric a...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-25-2-123

    authors: Stewart-Tull DE,Ollar RA

    更新日期:1988-02-01 00:00:00

  • Expression of prostaglandin receptors in Chlamydia trachomatis-infected recurrent spontaneous aborters.

    abstract::A study was undertaken to quantify the expression of prostaglandin (PG) receptors and find the effect of gestational age on expression of PG receptor genes in Chlamydia trachomatis-infected recurrent spontaneous aborters (RSA). Endometrial curettage tissue (ECT) was collected from 130 RSA (Group I) and 100 age-matched...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000256

    authors: Singh N,Prasad P,Singh LC,Das B,Rastogi S

    更新日期:2016-06-01 00:00:00

  • Age-related changes in the prevalence of precipitating antibodies to BK virus in infants and children.

    abstract::Nearly 1000 sera from children were tested by immunoelectro-osmophoresis against BK virus, and age-specific prevalence rates were estimated from birth until the age of 12 years. Declining rates during the first 12 months showed the waning of passive immunity, which at birth reflects the mother's immune status. The cha...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-15-3-285

    authors: Dei R,Marmo F,Corte D,Sampietro MG,Franceschini E,Urbano P

    更新日期:1982-08-01 00:00:00

  • Laboratory diagnosis of Clostridium perfringens antibiotic-associated diarrhoea.

    abstract::Clostridium perfringens has been reported as the cause of up to 15% of cases of antibiotic-associated diarrhoea (AAD) and may be diagnosed by detection of enterotoxin (CPEnt) in faeces. The performance of a commercial ELISA method for CPEnt, with culture and PCR methods to confirm the presence of enterotoxigenic C. pe...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-51-10-891

    authors: Asha NJ,Wilcox MH

    更新日期:2002-10-01 00:00:00

  • A collaborative evaluation of a rapid, semi-automated identification system for gram-negative bacilli: the Quantum II BID.

    abstract::The recently-introduced semi-automated bacterial identification system, the Quantum II BID, is designed to identify fermentative and non-fermentative gram-negative bacilli in 4-5 h. The system was evaluated independently by the two participating laboratories. Inter-laboratory reproducibility was determined by testing ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-21-2-145

    authors: Stevens M,Henrichsen C,Smith M

    更新日期:1986-03-01 00:00:00

  • Prospective evaluation of BDProbeTec strand displacement amplification (SDA) system for diagnosis of tuberculosis in non-respiratory and respiratory samples.

    abstract::Nucleic acid amplification techniques (NAATs) have been demonstrated to make significant improvements in the diagnosis of tuberculosis (TB), particularly in the time to diagnosis and the diagnosis of smear-negative TB. The BD ProbeTec strand displacement amplification (SDA) system for the diagnosis of pulmonary and no...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.45780-0

    authors: McHugh TD,Pope CF,Ling CL,Patel S,Billington OJ,Gosling RD,Lipman MC,Gillespie SH

    更新日期:2004-12-01 00:00:00

  • PCR evaluation of false-positive signals from two automated blood-culture systems.

    abstract::Rapid detection of micro-organisms from blood is one of the most critical functions of a diagnostic microbiology laboratory. Automated blood-culture systems reduce the time needed to detect positive cultures, and reduce specimen handling. The false-positive rate of such systems is 1-10%. In this study, the presence of...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.46196-0

    authors: Karahan ZC,Mumcuoglu I,Guriz H,Tamer D,Balaban N,Aysev D,Akar N

    更新日期:2006-01-01 00:00:00

  • Do English NHS Microbiology laboratories offer adequate services for the diagnosis of UTI in children? Healthcare Quality Improvement Partnership (HQIP) Audit of Standard Operational Procedures.

    abstract::The National Institute of Care Excellence (NICE) 2007 guidance CG54, on urinary tract infection (UTI) in children, states that clinicians should use urgent microscopy and culture as the preferred method for diagnosing UTI in the hospital setting for severe illness in children under 3 years old and from the GP setting ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.000114

    authors: McNulty CAM,Verlander NQ,Moore PCL,Larcombe J,Dudley J,Banerjee J,Jadresic L

    更新日期:2015-09-01 00:00:00

  • A simple and safe volumetric alternative to the method of Miles, Misra and Irwin for counting viable bacteria.

    abstract::A rapid and simple method for counting viable bacteria is described. The technique involves the use of a semi-automatic micropipette and disposable glass-capillary tubes. Viable counts obtained by this method were not significantly different from those obtained by the Miles, Misra and Irwin method or by a pour-plate m...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-11-4-541

    authors: Slack MP,Wheldon DB

    更新日期:1978-11-01 00:00:00

  • Susceptibility to beta-lactam antibiotics of mutant strains of Xanthomonas maltophilia with high- and low-level constitutive expression of L1 and L2 beta-lactamases.

    abstract::Xanthomonas maltophilia produces two inducible beta-lactamases, L1 and L2, and resists the antimicrobial activity of beta-lactam antibiotics, including carbapenems. L1 is a zinc-metaloenzyme with carbapenemase activity; L2 is an unusual cephalosporinase. Mutant strains with high- and low-level constitutive expression ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-35-4-208

    authors: Akova M,Bonfiglio G,Livermore DM

    更新日期:1991-10-01 00:00:00

  • Inhibition by amoeba-specific antiserum and by cytochalasin B of the cytopathogenicity of Naegleria fowleri in mouse embryo-cell cultures.

    abstract::Inhibitors of trophozoite motility and phagocytosis were used to investigate the mechanism of Naegleria fowleri cytopathogenicity in mouse-embryo (ME)-cell cultures. Amoebae that were immobilised and agglutinated by specific antiserum exhibited no cytopathic activity, although they remained alive and were in constant ...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-12-3-355

    authors: Brown T

    更新日期:1979-08-01 00:00:00

  • Characteristics of Lactobacillus and Gardnerella vaginalis from women with or without bacterial vaginosis and their relationships in gnotobiotic mice.

    abstract::The objectives of the present study were to evaluate in vitro the production of antagonistic compounds against Gardnerella vaginalis by Lactobacillus strains isolated from women with or without bacterial vaginosis (BV), and to select one of the better Lactobacillus producers of such a substance to be tested in vivo us...

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/jmm.0.041962-0

    authors: Teixeira GS,Carvalho FP,Arantes RME,Nunes AC,Moreira JLS,Mendonça M,Almeida RB,Farias LM,Carvalho MAR,Nicoli JR

    更新日期:2012-08-01 00:00:00

  • The prevalence of Chlamydia trachomatis in fresh tissue specimens from patients with ectopic pregnancy or tubal factor infertility as determined by PCR and in-situ hybridisation.

    abstract::The prevalence of chlamydial DNA determined by PCR and in-situ hybridisation (ISH) in fresh tissue specimens (endometrium, fallopian tube and ovary) was investigated in 33 women presenting with ectopic pregnancy (EP), 14 women with tubal factor infertility (TFI) and 50 control patients from the UK and the West Indies....

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/0022-1317-50-10-902

    authors: Barlow REL,Cooke ID,Odukoya O,Heatley MK,Jenkins J,Narayansingh G,Ramsewak SS,Eley A

    更新日期:2001-10-01 00:00:00

  • Characterisation of target antigen of cell-mediated immunity in Mycobacterium tuberculosis H37RV.

    abstract::Antigen participating in cell-mediated immunity was isolated from a sonic extract of Mycobacterium tuberculosis H37Rv. The purified antigen had a mol. wt 150 000-200 000, based upon its Rf in 7.5% polyacrylamide gel in the presence of sodium dodecyl sulphate. In simple agarose gel electrophoresis it had an Rf of 0.85....

    journal_title:Journal of medical microbiology

    pub_type: 杂志文章

    doi:10.1099/00222615-18-1-17

    authors: Hattikudur S,Kamat RS

    更新日期:1984-08-01 00:00:00